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Universal basal culture medium for zooblasts

A basal medium and animal cell technology, applied in the field of general-purpose basal medium for animal cells, can solve the problems of time-consuming and labor-consuming, difficult to choose, poor versatility, etc., achieve good versatility, and save time for screening medium , high practical effect

Inactive Publication Date: 2017-07-28
成都源泉生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is the problem of the versatility of the culture medium. The purpose is to provide a general-purpose basal medium for animal cells, so that it is difficult for experimenters to choose one suitable for their cell culture due to the wide variety of basal medium for cells but poor versatility. The time-consuming and labor-intensive problem of using culture medium

Method used

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  • Universal basal culture medium for zooblasts
  • Universal basal culture medium for zooblasts
  • Universal basal culture medium for zooblasts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027]The general-purpose basal medium for animal cells of the present invention includes components at the following concentrations: 30-50 mg / l alanine, 30-50 mg / l glutamic acid, 100-500 mg / l arginine, 39-50 mg / l Aspartic acid, 50-100mg / l asparagine, 30-50mg / l cystine, 100-500mg / l glutamine, 20-50mg / l glycine, 20-50mg / l histidine, 20 -50mg / l Hydroxyproline, 20-50mg / l Isoleucine, 50-100mg / l Leucine, 50-100mg / l Lysine, 30-50mg / l Methionine, 30-50mg / l Benzene Alanine, 30-50mg / l proline, 30-50mg / l serine, 30-50mg / l threonine, 20-50mg / l tryptophan, 50-100mg / l disodium tyrosine, 30 -50mg / l valine, 100-300mg / l calcium chloride, 50-100mg / l magnesium sulfate, 100-500mg / l potassium chloride, 30-50mg / l potassium dihydrogen phosphate, 500-1500mg / l phosphoric acid Disodium Hydrogen, 0.3-0.5g / l Sodium Chloride, 0.1-0.5mg / l Biotin, 5-10mg / l Choline Chloride, 30-50mg / l Inositol, 1-5mg / l Nicotinamide, 0.1 -0.5mg / l D-pantothenic acid hemicalcium, 3-10mg / l pyridoxal, 3-10mg / l thiamine, 0.1-1m...

Embodiment 2

[0030] Based on Example 1, the general-purpose basal medium for animal cells includes components at the following concentrations: 40mg / l alanine, 40mg / l glutamic acid, 300mg / l arginine, 45mg / l aspartic acid , 75mg / l Asparagine, 40mg / l Cystine, 300mg / l Glutamine, 35mg / l Glycine, 35mg / l Histidine, 35mg / l Hydroxyproline, 35mg / l Isoleucine , 75mg / l leucine, 75mg / l lysine, 40mg / l methionine, 40mg / l phenylalanine, 40mg / l proline, 40mg / l serine, 40mg / l threonine, 35mg / l Tryptophan, 75mg / l disodium tyrosine, 40mg / l valine, 200mg / l calcium chloride, 75mg / l magnesium sulfate, 300mg / l potassium chloride, 40mg / l potassium dihydrogen phosphate, 1000mg / l Disodium hydrogen phosphate, 0.4g / l sodium chloride, 0.3mg / l biotin, 7.5mg / l choline chloride, 40mg / l inositol, 3mg / l nicotinamide, 0.3mg / l D-pantothenic acid hemicalcium , 6.5mg / l pyridoxal, 6.5mg / l thiamine, 0.6mg / l riboflavin, 40mg / l ascorbic acid, 6.5mg / l folic acid, 4000mg / l D-glucose, 40mg / l thymidine, 5.5mg / l reduced glutathione....

Embodiment 3

[0032] Based on the above examples, such as figure 1 , figure 2 , shown in table 1, the present invention has carried out A549 cell in Gibco 1640, Gibco DMEM, the general-purpose animal cell of the present invention in the basal medium of the present invention 1x and the culture effect contrast under 2x cell inoculation secret degree, figure 1 , figure 2 The data shown are integrated in Table 1, and the source general-purpose basal medium in Table 1 is the general-purpose animal cell basal medium of the present invention; figure 1 , figure 2 , as shown in Table 1, the cell growth rate in the general-purpose animal cell basal medium of the present invention can reach and exceed the cell growth rate in Gibco 1640 and Gibco DMEM.

[0033] Table 1 Comparison of A549 cells cultured in three media

[0034] (Yuanquan universal basal medium Gibco 1640, Gibco DMEM)

[0035]

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Abstract

The invention discloses a universal basal culture medium for zooblasts. The universal basal culture medium for zooblasts comprises the following components: alanine, glutamic acid, arginine, aspartic acid, cystine, glutamine, glycine, histidine, hydroxyproline, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, disodium tyrosine, valine, calcium chloride, magnesium sulfate, potassium chloride, potassium dihydrogen phosphate, disodium hydrogen phosphate, sodium chloride, biotin, choline chloride, inositol, niacinamide, D-semi-calcium pantothenate, pyridoxal, thiamine, riboflavin, ascorbic acid, folic acid, D-glucose, thymidine and reduced glutathione. The universal basal culture medium for zooblasts is good in universality.

Description

technical field [0001] The invention relates to a culture medium, in particular to a general-purpose basal culture medium for animal cells. Background technique [0002] Cell culture medium is not only the basic material for supplying cell nutrition and promoting cell reproduction and proliferation in cultured cells, but also the living environment for the growth and reproduction of cultured cells. [0003] After decades of development, cell culture has become more and more mature, and is now widely used in biology, medical technology, pharmaceuticals and other fields. Gradually form basic media such as RPMI1640, DMEM, and F12 to meet the nutritional needs of different cells. However, there are many types of basic media. In cell culture, what kind of media should be selected for specific cell and stem cell culture often requires scientific research. Personnel test one by one, in order to finally determine which medium and which brand to choose. The result is time-consuming...

Claims

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Application Information

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IPC IPC(8): C12N5/07
CPCC12N5/0018C12N2500/05C12N2500/32C12N2500/35C12N2500/38C12N2500/40C12N2501/998
Inventor 刘才明
Owner 成都源泉生物科技有限公司
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