721results about How to "Reduce fermentation costs" patented technology

The invention discloses a Schizochytrium sp., which has a category name of Schizochytrium sp., is preserved in Common Microorganisms Center of China Committee for Culture Collection of Microorganisms and has a preservation number of CCTCC No.209059. The invention also discloses a method for producing DHA lipa by using the Schizochytrium sp. The method optimizes a strain fermentation medium from the perspectives of osmotic pressure and element supply and combines a fed-batch strategy to achieve high-density fermentation of microalgae, thus the final dry cell weight reaches 70 grams per liter, the lipa content reaches 31.5 grams per liter, and the DHA accounts for more than 35 percent of the total fatty acid content. All indexes of the DHA essential oil obtained by the method accord with the standard of food additives, and the DHA essential oil contains a bioactive substance of squalene. The method has the advantages of simple entire process, convenient operation, and high biomass and DHA content, reduces the fermentation cost, and is suitable for industrial production.

The invention discloses a bacillus licheniformis strain which is preserved as bacillus licheniformis WX-02 with the preserving number of NO: M208065 and the preserving date of April 24th, 2008 and the preserving unit of CCTCC. The invention also discloses an application of the bacillus licheniformis strain and a method for producing poly-gamma-glutamic acid by using the bacillus licheniformis strain. Poly-gamma-glutamic acid products are produced by processes of seed solution preparation and liquid submerged fermentation, and the strain has stable genetic performance of the strain, high production efficiency and low production cost. When the bacillus licheniformis strain of the invention and the fermentation method thereof are used for preparing 3000L of poly-gamma-glutamic acid, the yield of a fermentation tank can reach 35.05g / l.

The invention relates to a preparation method of an electric spinning-based composite nano fiber material for filters, comprising the following steps of: preparing an electric spinning nano fiber film with reliable quality by adopting a polymer solution or a fusant through an electrostatic spinning device, and combining the electric spinning nano fiber film with a conventional filtering material to form a sandwich composite nano filtering material. Compared with the prior art, the nano fiber film with reliable quality in the invention can filter particles of 1 micrometer, and the filtering efficiency is 100%; the conventional filtering material and coarse fibers not only are used as supporting materials, but also can filter larger particles in advance, and the preparation method reduces the abrasion of the nano fiber film, prolongs the service life of the composite nano filtering material, reduces the updating time of the composite nano filtering material, and lowers filtering expenses.

A method for producing L-lactic acid by Bacillus coagulans CGMCC No.2602 belongs to the technical field of microorganisms. In the invention, Bacillus coagulans CGMCC No.2602 is adopted, and under the condition of no oxygen supply, starchiness hydrolyzed sugar or dextrose is fermented by a semicontinuous intermittent fermentation way or an inter-sugar-compensating fermentation way to generate L-lactic acid with high optical purity. The invention has the advantages that the gemma property of the Bacillus coagulans is stable, and the L-lactic acid obtained by inoculating and fermenting the starchiness hydrolyzed sugar has high optical purity and rate of conversion of sugar and acid and short fermentation period; in addition, the semicontinuous intermittent fermentation way saves the time for preparing seeds by a continuous reladling and subculturing method, shortens the fermentation period, enhances the fermentation strength and obtains the L-lactic acid with both relatively high rate of conversion of sugar and acid and purity.

The invention relates to lactobacillus reuteri and application thereof. Lactobacillus reuteri is lactobacillus reuteri LZ262 which is preserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms on 13 August, 2015 with the preservation number of CGMCCNo.11240. Lactobacillus reuteri LZ262 is from the intestinal tract of a healthy pig, can be colonized in a human body and an animal body, and has higher tolerance to gastric acid and bile salt; in addition, lactobacillus reuteri LZ262 has a better suppression effect on Escherichia coli, and has a higher survival rate after spray-drying, and higher fermentation density is achieved by improving a fermentation formula; the strain has a better effect of synergistic growth with other types of lactic acid bacteria, can be used for food and animal feed additives, and can also be widely applied to other food and biological products.

The invention discloses microbial fermentation feed as well as a production method and an application thereof. The production method of the microbial fermentation feed comprises the following steps: (1) lactic acid bacteria, Clostridium butyricum Cb-2, yeast, Bacillus subtilis, a fermentation base-material and water are uniformly mixed, and a fermentation raw material is obtained; 2) aerobic fermentation and anaerobic fermentation are carried out in order for the fermentation raw material obtained in the step (1), and after fermentation, the feed is obtained. The microbial fermentation feed contains microecological viable organisms, and the total amount of beneficial bacteria is more than 5 x 10<9> cfu / g; the amount of mould and the content of mould toxin are lower than national limit standards; the feed is rich in organic acid, the content of lactic acid reaches 2.5%, and pH value is lower than 4.5; the titer of antibacterial peptide is higher than 1000 u / g, the titer of neutral protease is 150 u / g, the titer of alpha-amylase 300 u / g, dry weight for above, and the titer of total protease is higher than 600 u / g; the whole fermentation process is controlled in the range of 8-24 hours, the fermentation time is saved, and the dissipating energy is controlled below 3%.

A process for preparing 1,3-propanediol biologically features that in the culture medium for fermenting or in the anaerobic fermenting procedure, appropriate reducer is added to increase the accumulation of reduction equivalent weight in thallus for promoting metabolism of glycerine along the reductino approach, and increasing the concentration and transform rate of 1,3-propanediol. Its advantageis high biologic utilization rate.

The invention relates to a method for treating threonine mother liquor, and concretely relates to the method for treating other waste liquid by using compound enzyme to perform enzymolysis and fermentation of waste thallus and by using a bipolar membrane electrodialysis treatment. According to the invention, the problem of effective components separation on threonine mother liquor, threonine fermentation residual sugar and other components are fully utilized, the method of the invention has the advantages of high energy efficiency, less waste water emission and low pollution; in addition, an electrodialysis technology has the advantages of simple operation, no pollution, long service life and the like, thereby the investment cost of enterprises can be reduced, the energy saving and emission reduction can be carried out, so that the method of the invention serves multiple purposes.

The invention relates to saccharification processing technology of jerusalem artichoke by utilizing high-quality fructose biomass as a raw material and strain selection and technology optimization for producing mannitol by taking jerusalem artichoke as carbon source through fermentation. The method comprises the following steps: 1) crushing jerusalem artichoke tuber into coarse particles, filtering after water leaching and enzymolysis for 6 hours, supernating at 42 DEG C, rotating, evaporating and concentrating to obtain saccharification jerusalem artichoke juice with high concentration of fructose; 2) establishing high performance liquid chromatography analysis and detection conditions which can synchronously analyze the content of fermentation liquor substrate (glucose and fructose) and products (mannitol); and 3) inspecting the capacity for producing lactic acid and mannitol through fermentation by seven lactic acid bacteria by utilizing saccharification jerusalem artichoke juice with different concentration of total sugar, thus determining lactic acid bacteria with high transformation rate and production intensity of fructose, and optimizing production fermentation conditions and the highest concentration of tolerant substrate. Through feed-batch fermentation, production efficiency can be improved and mannitol can be continuously produced in large scale. The method not only generates no byproduct of sorbitol, but also has low production cost, wide raw material sources, simple technology, and mature technical route and can be implemented in industrialization.

A process for preparing arachidonic acid oil by microbial fermenting includes culturing and activating Mortierella alpina (M0223), culturing seed, inoculating the seed in shake flask or fermentor, fermenting, collecting wet thallus, baking and extracting oil. Its advantages are high purity and safety.

The invention relates to a method for preparing ocean rhodotorula fermentation feed. The method comprises the preparation of solid fermentation base stock of soybean meal, the mixing of a nutrition replenishing agent and the preparation of a solid fermentation medium. The feed is characterized in that ocean rhodotorula and a lactobacillus strain are inoculated in the solid fermentation medium and are subjected to solid fermentation to obtain the ocean rhodotorula fermentation feed. The method has the advantages: 1. macromolecular protein in the soybean meal is decomposed into small molecular peptide which improves the digestive absorption rate of the protein and further balances the composition of amino acid through microbial transformation; 2. the fermentation feed is replenished with astaxanthin and other biological active substances introduced by the metabolism of rhodotorula glutinis, thereby improving the nutritive value of the feed; 3. lactobacillus in the fermentation feed has positive function on improving a microecological flora of intestinal tracts of bred animal; and 4. the method has low fermentation cost, low fermentation condition and equipment requirement, simple operation of process flow and small investment and is suitable for small-scale production of breeding households and feed processing factories.

The invention relates to culture mediums for producing doramectin through fermentation of mutant streptomyces avermitilis and a material supplement method. The culture mediums comprise a seed culture medium and a fermentation culture medium, and are characterized in that the seed culture medium comprises corn lees and low-temperature pressing soya bean cake powder; the fermentation culture medium comprises corn lees, low-temperature pressing soya bean cake powder, polystyrene non-polar adsorbent resins and nonionic surfactants. The cost problem of raw materials and auxiliary materials are solved, the environment influence on sources of the raw materials and the auxiliary materials is reduced to the hilt, the supplying of the raw materials and the auxiliary materials is sufficient, the stable and efficient production of the doramectin is realized, and at the same time, the fermentation unit can be improved by using the culture mediums and the material supplement method.

The invention discloses a fermentation production method based on epothilone B metabolic pathways and belongs to the technical field of fermentation engineering. The method comprises the following steps of: 1) inoculating Sorangium cellulosum into an M26 culture medium, carrying out shake cultivation to obtain a seed solution; 2) inoculating the seed solution into fermentation liquor containing resin, carrying out shake culturing for 72-120 hours at 25-35 DEG C, adding a precursor and small molecular substances into the fermentation liquor containing resin; and 3) filtering the fermentation liquor after the culture is finished, collecting the resin, washing the resin and oscillating and extracting with methyl alcohol to obtain extract liquor containing the epothilone B. According to the invention, the precursor and small molecular substances related to the biosynthetic pathways obtained by screening are added into the fermentation liquor, and the constructive metabolism pathways of the epothilone B are disturbed, so that the excellent bacterial strain gives full play to the ability of anabolism and the fermentation level of the epothilone B is improved greatly, thus the fermentation cost is reduced and the commercial process of the anti-cancer drug is promoted.

The invention discloses a yoghourt fermenting agent and a preparation method thereof. The yoghourt fermenting agent consists of two kinds of freeze-dried bacterium powder namely lactobacillus delbrueckii subspecies bulgaricus (Lactobacillus delbrueckii subspecies bulgaricus) NJ 441 and streptococcus thermophilus (Streptococcus thermophilus ) NJ21, wherein the NJ 441 has the main effect of acid production, so that the pH value of the yoghourt obtained through fermentation of the NJ 441, is slightly higher than that of the yoghourt which is obtained through fermentation of lactobacillus delbrueckii subspecies bulgaricus sold in the market, and the differences are reflected in the mouth feel that the yoghourt which is obtained through the fermentation of bacteria disclosed by the invention is sour and soft rather than irritant or strong; fermentation experiments are performed on milk through the NJ21 in a composition, people find that the yoghourt which is obtained through the fermentation of the NJ21 is glutinous and soft in texture and just melts in the mouths, the texture and the mouth feel of the yoghourt are improved, besides, the curding time is short, the production efficiency is improved, and the fermentation cost is reduced.

The invention provides a microbial deodorant and strain for eliminating waste odor of municipal landfill and waste transfer station, and the preparation and application method thereof. The preparation method comprises the following steps: culturing Saccharomyces cerevisiae with the conservation number of CGMCC No.5183 in a PDA culture medium, and culturing Lactobacillus casei (CGMCC No.5182) in an MRS culture medium to obtain the seed liquid; and fermenting in a fermentation tank to obtain the microbial deodorant. The microbial deodorant is prepared by a mixed culture manner so as to lower the fermentation cost, reduce the generation of mixed bacteria and stabilize the product quality. The microbial deodorant prepared by the method can effectively eliminate the waste odor of the municipallandfill so as to achieve good deodorant effect.

The invention discloses a method for cultivating high-yield DHA (docosahexaenoic acid) through schizochytrium, and belongs to the field of microbial fermentation. The method comprises steps as follows: a schizochytrium seed solution with the dry cell weight being 18-22 g / L after culturing in a shake flask is inoculated into a seed tank and then inoculated into a fermentation tank after being cultured to reach an inoculation transfer level, high-density culture is performed at the high temperature being 22 DEG C-33 DEG C in an early stage, induction culture is performed in a threatening manner for 110-130 hours at the low temperature being 18 DEG C-22 DEG C in a late stage, and glucose and total nitrogen are kept in a certain concentration and pH (potential of hydrogen) is kept ranging from 5.5 to 6.6 through feeding of a glucose solution, a nitrogen source solution and an alkaline solution in the fermentation process. The dry cell weight of a fermentation solution obtained with the method reaches 120g / L-150 g / L, the density of the DHA reaches 40g / L-50 g / L and is 1.2-1.5 times higher than reported DHA productivity, and the method is very suitable for industrial production of DHA.

The invention provides a method for preparing lycopene by using bacteria fermentation. The employed bacteria is Streptomyces rimosus sub.rimosus. Compared with current normal method, the invention is characterized by short fermentation period, simple system, low cost, high product purity, and suitability for industrial production.

The invention relates to a separation process for magnetic red mixed ore based on iron material disseminated particle size treatment. The process comprises the operation of feeding the mixed ore withthe grade of 27%-29% and with magnetite as a main raw material into first-section closed circuit ore grinding operation to obtain a primary grading overflow product, and is characterized by further comprising first-section weak magnetic and strong magnetic operation, fine screening operation, first-section magnetic separation and first-section washing magnetic separation operation, second-sectionclosed circuits ore grinding operation, second-section weak magnetic strong magnetic operation, second-section magnetic separation and washing magnetic separation operation, third-section closed circuit ore grinding, third-section weak magnetic strong magnetic operation and reverse flotation operation. The final concentrate with the grade of 66%-66.5% is obtained through the operation. Compared with the prior art, the separation process has the advantages that 1) an efficient separation process which is characterized in that the concentrate can be obtained once dissociation is achieved and segmented ore grinding, segmented concentrate taking and segmented tailings discharging are achieved is provided for the magnetic red mixed ore with the non-uniform iron material disseminated particle sizes, so that overgrinding is reduced, and energy conservation and consumption reduction are achieved; 2) magnetic separation equipment is adopted for upgrading, and three sections of ore grinding is carried out by adopting vertical mills; and 3) the process is simple in structure, free of middling circulation, easy to implement on site and easy to produce and manage.

The invention relates to a method for propagation culture and solid fermentation of white-rot fungi, which belongs to a method for fungal solid fermentation and comprises the following steps: activation of strains; liquid propagation culture; fermentation tank liquid propagation culture; and solid culture and domestication in a canned bottle, and fermentation in a fermentation tank, wherein the inoculum sizes of the stage of the solid culture and domestication in the canned bottle and the stage of the fermentation in the fermentation tank are 10 percent and 5 percent respectively. The method has the following advantages: the method has simple technical process so as to be directly applied to large-scale fermentation tanks in factories, has strong the pertinence so as to be applied to sewage treatment and mixed white-rot fungi fermentation in the paper industry, and has high use value, high fermentation efficiency, low fermentation cost and the like.

The invention relates to a method for preparing a kbac beverage. The method comprises the following steps: adding water into a saccharifying tank, heating to be 35 DEG C, adding breadcrumbs, uniformly stirring, adding malt flour, heating to be 65 DEG C, adding alpha-amylase, heating to be 90 DEG C, stopping stirring, heating to be 100 DEG C, keeping the temperature for 20 minutes, cooling to be 52 DEG C, adding beta-glucanase, neutral protease and malt flour, keeping the temperature for 70 minutes, heating to be 60 DEG C, adding saccharifying enzyme, heating to be 63 DEG C, keeping the temperature for 40 minutes, heating to be 68 DEG C, keeping the temperature for 20 minutes, heating to be 78 DEG C, standing for 60 minutes to obtain a saccharification liquid, and filtering to obtain supernate. On the basis that the kbac fermented beverage prepared by using the method provided by the invention does not affect the original rich nutrition and health value, the biologic stability of a product is greatly improved, and the product is better in stability and taste within the expiration date. The product stability is that the nutrient substance can be separated for 180-200 days increased from 3-40 days.

The invention discloses a fermentation method for improving the yield of isoleucine. The fermentation method comprises the following steps: culturing microorganisms, fermenting, separating fermentation liquor, carrying out ion exchange on fermentation supernatant, deaminizing with a deamination tower, decolorizing with activated carbon and crystallizing by evaporating. Because the corn steep liquor which serves as a hydrolysis liquor in the culture medium and accounts for 40% of the total weight of the culture medium replaces the hydrochloric acid adopted by the traditional method to regulate the pH value, the corrosion of the hydrochloric acid to the stainless steel tank is avoided. In addition, because biotin and VB1 are used as growth factors in the process, the viscosity of the fermentation liquor can be reduced, the respiratory metabolism of the microorganisms can be promoted, and the down-stream finished product can be extracted easily. By adopting the fermentation method disclosed by the invention, a good fermentation index can be achieved, the yield of the isoleucine which is a metabolite can be improved significantly, the average yield of the isoleucine can be up to 36.9g / L and can be increased by 30% compared with that of the metabolite isoleucine obtained by adopting the original method, the conversion rate of the metabolite isoleucine can be increased by 2-4%, and the fermentation cycle can be shortened by 10 hours.

The invention relates to a production method and application of a high-efficient cellulase mixture. Through performing fermentation by using a non-soluble carbon source, not only can fermentation cost of cellulase be saved, but also the production of the cellulase is effectively induced; a reducing sugar control measure is adopted, so that the repression effect of a carbohydrate is reduced, and the production capacity of the cellulase is improved; in addition, a novel material-making up control manner is built through combining the non-soluble carbon source with a material-making up component and a material-making up rate process, so that matching of all components of the produced cellulase mixture is optimized while the yield (production capacity) of the cellulase is promoted, and the activity of the cellulase is improved by not less than 50 percent.

The invention belongs to the field of microbial fermentation and in particular relates to a method for preparing taste-active peptides and delicious amino acids by using aspergillus oryzae fermented peanut meal and application thereof. The method comprises the following steps: performing ultraviolet mutagenesis by utilizing aspergillus oryzae AS 3.95, and screening to obtain a mutant strain of which the protease activity is improved; and performing peanut meal fermentation by utilizing the strain so as to produce the peanut peptides and delicious amino acids with good flavors. The hydrolysis rate and delicate flavor value of the peanut proteins can be improved, the content of taste-active peptides and free amino acids is increased, and the method has high application value in flavoring base materials. The main component of the selected fermentation medium is peanut meal, the raw materials are easily available and low in price, and the cost is low. The hydrolysis rate and delicate flavor value of the peanut meal proteins can be obviously improved after fermentation, and the method has high application value. According to the preparation method disclosed by the invention, the fermentation cost can be reduced, the production link is simplified, and the efficiency is improved.

The invention relates to the field of microorganisms, in particular to a biocontrol bacterium for efficiently producing lipopeptide substances. The biocontrol bacterium is Bacillus velezensis HN-2 andpreserved in the China Center for Type Culture Collection on June 20th, 2018, and the preservation number is CCTCC NO:M 2018382. The invention also discloses a fermentation technology of the Bacillusvelezensis HN-2. It is found through activity testing that an extract of fermentation broth of the strain has a protective function on tobacco when the tobacco mosaic virus (TMV) infects the tobacco,the tobacco defense reaction can be induced, the resistance of the tobacco to the TMV is improved, and the economic benefits are relatively high.

The invention discloses a method for improving the poly-malic acid yield in aureobasidium pullulans through surface active agent tween or span. The method particularly comprises the steps that after the aureobasidium pullulans is activated, the aureobasidium pullulans is fermented under the condition containing 0.1 g / L to 0.5 g / L tween or span. The method is simple, the tween or the span can be directly added into a fermentation medium, the carbon metabolism flow direction of synthesis of the poly-malic acid and Pulullan can be adjusted, the poly-malic acid yield can be improved to a large extent, and the method has the advantages of being low in fermentation cost, high in yield, strong in technical economical efficiency, capable of being applied to poly-malic acid industrial production and the like.

The invention provides a method for preparing alcohol and biochar from traditional Chinese medicine residues in a gradient mode and belongs to the technical field of biomass waste resource utilization. According to the method, traditional Chinese medicine residue dilute alkali is preprocessed, matter rich in nitrogen is added for alcohol fermentation, alcohol fermentation solid residues are subjected to pyrolysis in a high-temperature tube furnace at the nitrogen atmosphere, and biochar is prepared. The method for using the traditional Chinese medicine residues in the gradient mode is implemented, the alcohol yield of lignocellulose traditional Chinese medicine residues can be increased by one to three times through alcohol fermentation, the methylene blue adsorption rate is increased by 20-30%, and cellulose and lignin can be used more effectively. Resource utilization of a lignocellulose raw material is achieved, the method has important significance in widening the solid waste application range and developing new sources of alcohol and biochar, alcohol fermentation cost is reduced, the problem about biomass energy high-additional-value utilization is solved, and the method has good economic benefits and huge social benefits.

The invention provides a preparation method for lactobacillus plantarum used for a feed. To overcome the problems of abuse of antibiotics in farming, generation of drug resistance and the like at present, application of a novel antibiotic substitute product, i.e., probiotics, is a trend of modern farming. The probiotics is prepared through deep liquid state fermentation, has the advantages of low production cost and a high viable count and is a stable, highly-efficient and green feed additive.