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Biocontrol bacterium HN-2 for efficiently producing lipopeptide substances and fermentation technology thereof

A fermentation process and bio-control bacteria technology, applied in the field of microorganisms, can solve the problems of loss of antibacterial ability, inability to synthesize lipopeptide substances, inability to synthesize 4′-phosphopantetheinyl transferase, etc., to improve resistance Effect

Active Publication Date: 2019-08-13
HAINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The strain has complete surfactin coding genes srfA, srfB, srfC and srfD, etc., but due to the spontaneous mutation of the sfp gene of B168, the 4′-phosphopantetheinyltransferase synthesized by the sfp gene cannot be synthesized, further making the surfactin The synthetase cannot be activated, resulting in the inability of the B168 strain to synthesize lipopeptides and the loss of antibacterial ability

Method used

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  • Biocontrol bacterium HN-2 for efficiently producing lipopeptide substances and fermentation technology thereof
  • Biocontrol bacterium HN-2 for efficiently producing lipopeptide substances and fermentation technology thereof
  • Biocontrol bacterium HN-2 for efficiently producing lipopeptide substances and fermentation technology thereof

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Embodiment Construction

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0031] 1. Identification of strains

[0032] (1) Morphological identification

[0033] The single colony of HN-2 strain on LB solid medium was translucent and milky white in the early stage, and the surface was dry in the later stage, with wrinkles in the middle and irregular edges. Purple after Gram staining, for Gram-positive bacteria. Bacteria are rod-shaped with a single flagella.

[0034] (2) 16srDNA analysis

[0035] Using the HN-2 strain DNA as a templat...

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Abstract

The invention relates to the field of microorganisms, in particular to a biocontrol bacterium for efficiently producing lipopeptide substances. The biocontrol bacterium is Bacillus velezensis HN-2 andpreserved in the China Center for Type Culture Collection on June 20th, 2018, and the preservation number is CCTCC NO:M 2018382. The invention also discloses a fermentation technology of the Bacillusvelezensis HN-2. It is found through activity testing that an extract of fermentation broth of the strain has a protective function on tobacco when the tobacco mosaic virus (TMV) infects the tobacco,the tobacco defense reaction can be induced, the resistance of the tobacco to the TMV is improved, and the economic benefits are relatively high.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a biocontrol bacterium HN-2 with high-efficiency lipopeptide production and a fermentation process. Background technique [0002] tobacco mosaic virus ( tobacco mosaic virus , TMV) caused by mosaic virus disease is a tobacco disease that commonly occurs in various tobacco-growing areas around the world, and is seriously prevalent in some areas. When the tobacco disease occurs, the leaves are uneven in thickness, yellow and green in color, and are in the shape of mosaics. The mottled degree of the back mosaic leaves increases, and large areas of dark brown necrotic spots appear, especially in the middle and lower parts of the old leaves. The severely affected leaves are shrunken, deformed, and twisted. The current methods of preventing and controlling tobacco mosaic virus disease are mainly through field management and cultivation of disease-resistant varieties. The treatment effe...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P21/02A01N63/02A01P1/00C12R1/07
CPCC12P21/02C12R2001/07C12N1/205A01N63/10
Inventor 靳鹏飞王雨缪卫国刘文波王皓楠谭峥
Owner HAINAN UNIVERSITY
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