Compositions, methods, strategies, kits, and systems for the supercharged
protein-mediated delivery of functional
effector proteins into cells
in vivo,
ex vivo, or
in vitro are provided. Compositions, methods, strategies, kits, and systems for delivery of functional
effector proteins using cationic lipids and cationic polymers are also provided. Functional
effector proteins include, without limitation, transcriptional modulators (e.g., repressors or activators), recombinases, nucleases (e.g.,
RNA-programmable nucleases, such as Cas9 proteins; TALE
nuclease, and
zinc finger nucleases), deaminases, and other
gene modifying / editing enzymes. Functional effector proteins include TALE effector proteins, e.g., TALE transcriptional activators and repressors, as well as TALE nucleases. Compositions, methods, strategies, and systems for the delivery of functional effector proteins into cells is useful for therapeutic and research purposes, including, but not limited to, the targeted manipulation of a
gene associated with
disease, the modulation of the expression level of a
gene associated with
disease, and the
programming of
cell fate.