38 results about "Spontaneous mutation" patented technology

The present invention includes compositions and methods for making and using halophilic cyanobacterium comprising a spontaneous mutation causing constitutive cellulose biosynthesis, whereby the cyanobacterium is capable of producing cellulose in brine. The compositions and methods of the present invention may be used as a new global crop for the manufacture of cellulose, CO2 fixation, for the production of alternative sources of conventional cellulose as well as a biofuel and precursors thereof.

Belonging to the technical field of bioengineering, the invention relates to a genetic engineering strain producing succinic acid and a method for production of succinic acid by fermenting the strain, particularly a recombinant strain efficiently utilizing cane sugar and molasses to grow and produce succinic acid. The genetic engineering strain producing succinic acid is classified and named as Escherichia coli BA501, and has a preservation registration number of CCTCC NO:M2014014. The construction process of the strain mainly includes: taking Escherichia coli AFP111 that lacks lactate dehydrogenase gene and pyruvate formate lyase activity and has the chromosome ptsG gene undergoing spontaneous mutation as the starting strain, expressing exogenous sucrose permease, sucrose hydrolase and fructokinase genes, and then carrying out continuous domestication cultivation to obtain the strain efficiently utilizing cane sugar and molasses to grow and produce succinic acid. Thus, the synthesis efficiency of succinic acid is greatly improved. The fermentation method adopts a two-stage fermentation way, in the aerobic stage the biomass is improved oxygen, and in the anaerobic stage, fermentation and acid production are achieved.

A new and distinct variety of landscape shrub rose plant is provided which forms in abundance on a substantially continuous basis attractive single blossoms that are pink in coloration. The new variety is a spontaneous mutation of unknown causation of the `Radrazz` variety (U.S. Plant Pat. No. 11,836) that forms vivid red single blossoms. The vegetation is vigorous and the growth habit is compact and mounding. Attractive ornamental satiny green foliage is formed. Excellent disease resistance to blackspot is exhibited. The new variety is particularly well suited for growing as distinctive ornamentation in the landscape.

The invention discloses a genetic engineering strain capable of producing succinic acid at a low pH value. The classification name of the genetic engineering strain is Escherichia coli BA601, and the collection number is CCTCCNO: M2014210. The invention further discloses a method for producing succinic acid by using the strain. According to the strain disclosed by the invention, an Escherichia coli AFP111 which is lack of lactate dehydrogenase (LDH) genes and has activity of pyruvate formate lyase (PFL) genes and ptsG spontaneous mutation of a phosphotransferase system is taken as a starting strain for over-expression of acid-resistant genes gadBC. The method of enabling the Escherichia coli AFP111 which can not grow under low-pH (pH 5.6) conditions originally and can not produce acid by fermentation to efficiently utilize glucose to produce succinic acid through a biological and microbial acclimation means is not disclosed before, and the application can greatly promote the progress and development of the succinic acid industry.

A new and distinct variety of climbing rose plant is provided which forms in abundance on a substantially continuous basis attractive clusters of semi-double informal clear yellow blossoms having approximately ten petals on average. The new variety is a spontaneous mutation of unknown causation of the ‘Radsun’ variety (U.S. Plant Pat. No. 13,063). The climbing growth habit can be readily distinguished from the free-standing growth habit of the ‘Radsun’ variety. Vigorous vegetation is formed. The foliage is dense medium green with a satin finish. Excellent resistance to Black Spot is displayed. Attractive dense ornamentation in the form of foliage and blossoms is made possible when the new variety is grown on a support.

The invention discloses a cordyceps militaris rejuvenation breeding process. The concrete steps are as follows: carrying out activation culture of a degraded cordyceps militaris strain; inoculating the activated cordyceps militaris strain in a shake flask culture medium for culturing, and the inoculating the strain on a wheat or rice culture medium for culturing; selecting fruiting bodies, cuttingoff tips, hanging the tips on the cup of the cultivation culture medium for culturing until spores on the surface of the culture medium are germinated thoroughly, removing the fruiting body tips, andcontinuing to culture the tips for 30-40 d; and picking and drying the mature cordyceps militaris, detecting cordycepin, the main active ingredient of the cordyceps militaris, and using the cordycepsmilitaris as an original cultivation species until the traits are stable. The cordyceps militaris rejuvenation breeding process does not need to use a spore collector, is simplified in operation andsaves breeding cost. Furthermore, since the process is monospore purification rejuvenation, the purity of the strain can be guaranteed. The excellent strains with stable traits are orientedly screenedas the original cultivation species, and are suitable for the rejuvenation of strains degraded by spontaneous mutation and inappropriate environmental factors.

The invention concerns methods for the development of mutant animals, including genetically engineered animals and those carrying spontaneous mutations, as human disease models. In particular, the invention provides an integrated technology, including rigorous specifications and quality control, for the development of animal models that can serve as a living assay system, useful in biomedical research and in the development of human therapeutics.

Belonging to the technical field of bioengineering, the invention relates to a genetic engineering strain producing succinic acid and a method for production of succinic acid by fermenting the strain, particularly a recombinant strain efficiently utilizing cane sugar and molasses to grow and produce succinic acid. The genetic engineering strain producing succinic acid is classified and named as Escherichia coli BA501, and has a preservation registration number of CCTCC NO:M2014014. The construction process of the strain mainly includes: taking Escherichia coli AFP111 that lacks lactate dehydrogenase gene and pyruvate formate lyase activity and has the chromosome ptsG gene undergoing spontaneous mutation as the starting strain, expressing exogenous sucrose permease, sucrose hydrolase and fructokinase genes, and then carrying out continuous domestication cultivation to obtain the strain efficiently utilizing cane sugar and molasses to grow and produce succinic acid. Thus, the synthesis efficiency of succinic acid is greatly improved. The fermentation method adopts a two-stage fermentation way, in the aerobic stage the biomass is improved oxygen, and in the anaerobic stage, fermentation and acid production are achieved.

A new and distinct Hybrid Tea rose plant is provided which forms attractive blossoms that are hot pink in coloration. The new variety is a spontaneous mutation of unknown causation of the ‘Meilavio’ variety (U.S. Plant Pat. No. 10,845) that forms vivid red blossoms. The blossoms are fully double, long lasting, and very quartered in the sense the petals of the fully opened blossoms tend to be arranged in a plurality of zones when viewed from above. The vegetation is strong and vigorous and the growth habit is erect. Many large thorns are exhibited. The foliage is very decorative, dark green, and semi-glossy and contrasts nicely with the hot pink blossom coloration. Good resistance to Black Spot and Powdery Mildew is displayed. The new variety is well suited for providing attractive ornamentation in the garden.

A new and distinct variety of climbing rose plant is provided which forms in abundance on a substantially continuous basis attractive clusters of semi-double informal clear yellow blossoms having approximately ten petals on average. The new variety is a spontaneous mutation of unknown causation of the ‘Radsun’ variety (U.S. Plant Pat. No. 13,063). The climbing growth habit can be readily distinguished from the free-standing growth habit of the ‘Radsun’ variety. Vigorous vegetation is formed. The foliage is dense medium green with a satin finish. Excellent resistance to Black Spot is displayed. Attractive dense ornamentation in the form of foliage and blossoms is made possible when the new variety is grown on a support.

The application belongs to the technical field of microbial fermentation engineering, and specifically relates to genes related to the production of polyketide actin (ACT) in streptomyces. This application specifically relates to the application of stress, signal and transcription regulation genes in the regulation of Streptomyces ACT production. There are 14 stress, signal and transcription regulation genes in total; mutations of such genes have regulatory effects on the ACT production of Streptomyces. The inventor carried out mutagenesis on Streptomyces coelicolor M145, and screened the strains with changed actin rhodopsin production. The position of the transposon on the genome was located by enzyme digestion, self-ligation and sequencing of the screened positive clones, and the principle of multiple screening to locate the same gene was used to exclude the spontaneously mutated gene, and finally determine the factor affecting the production of actinhodine. Gene.

The present invention discloses new live attenuated strains for oral immunization against cholera that are provided in freeze dried formulations for long term storage and administration to humans. These strains combine the two most important properties of live attenuated cholera vaccine candidates. One such property is being well tolerated by people ingesting them. This was achieved by virtue of mutations already described in the art. The second property is having enhanced environmental safety due to the absence of VGJΦ DNA in their genomes and also due to null mutations in the mshA gene or other spontaneous mutations conducive to the lack of MSHA type IV fimbria on the bacterial surface. This was done envisioning that VGJΦ is a filamentous phage able to recombine with CTXΦ and disseminate the cholera toxin genes. This VGJΦ phage as well as the VGJΦ-CTXΦ recombinants uses the MSHA fibers as receptor. Being devoid of MSHA fimbria the vaccine candidates are protected from acquiring CTXΦ from the recombinant hybrid VGJΦ-CTXΦ. Being devoid of VGJΦ, the vaccine candidates are impaired in the dissemination of CTXΦ, via VGJΦ.

The application belongs to the technical field of microbial fermentation engineering, and specifically relates to genes related to the production of polyketide actin (ACT) in streptomyces. This application specifically relates to the application of the transporter gene in the regulation of Streptomyces ACT production, and there are 6 genes encoding the transporter; including: SCO2254 , SCO2534 , SCO3765 , SCO6160 , SCO2519 , SCO3185 ; After the mutation of this type of gene, it has a regulation effect on the production of Streptomyces ACT. The inventor carried out mutagenesis on Streptomyces coelicolor M145, and screened the strains with changed actin rhodopsin production. The position of the transposon on the genome was located by enzyme digestion, self-ligation and sequencing of the screened positive clones, and the principle of multiple screening to locate the same gene was used to exclude the spontaneously mutated gene, and finally determine the factor affecting the production of actinhodine. Gene.

The invention belongs to the technical field of plant genetic engineering. In particular, it relates to a rapid positioning and cloning method of a plant spontaneously mutated gene bridged by a neutral mutant. The purpose of the present invention is to overcome the defects of the prior art, and quickly locate the spontaneous mutant gene by utilizing the neutral mutant produced by mutagenesis with the EMS mutagen composition. F 1 , continue selfing to get F 2 generation. by screening F 2 The extreme recessive phenotypes in the population were mixed and sequenced, combined with the sequencing data of the neutral mutants induced by EMS, and the bioinformatics method was used to quickly locate the mutant genes. Compared with the known method, the present invention has the outstanding advantages of high screening efficiency, short time and less cost. The invention can be used for rapid cloning of spontaneously mutated genes.

The invention discloses an anti-bacteriophage aspartase variant production bacterium, a breeding method and an application thereof. The present invention carries out continuous spontaneous mutation breeding on the aspartase variant producing bacteria pL181 which is easily polluted by phages, and then utilizes the low-energy ion implantation mutagenesis method to efficiently breed a strain of aspartase with phage resistance Acidase variant producing bacteria Escherichia coli CGMCC NO.18005. The strain not only has the resistance to bacteriophage (CGMCC NO.17999), but also has 31% higher enzyme activity than the original strain pL181. While improving the enzyme-producing ability of the producing bacteria, the present invention fundamentally solves the difficult problem that β-alanine is easily infected by phages in the production, and improves the economic benefits of β-alanine production enterprises.

A new and distinct variety of landscape shrub rose plant is provided which forms in abundance on a substantially continuous basis attractive red-purple blossoms commonly bearing approximately 13 to 15 petals. The new variety is a spontaneous mutation of unknown causation of the ‘Radrazz’ variety (U.S. Plant Pat. No. 11,836) that forms vivid red blossoms which commonly display approximately 10 petals. The vegetation is vigorous and the growth habit is bushy and rounded. The foliage is very ornamental, dark green, and semi-glossy and contrasts nicely with the red-purple blossom coloration. Excellent resistance to blackspot is displayed. The new plant is particularly well suited for pot forcing under greenhouse growing conditions to form an attractive gift plant.

The invention discloses a method for reducing a spontaneous mutation background of wild type human-rat hybridoma AL cells. The method comprises the following steps: collecting wild type cells; combining with a fluorescent antibody; preparing a sampling suspension solution; sorting the cells; and preserving. The method is simple to operate; the whole process from the collection of the cells and the combination with specific fluorescent proteins to sorting only needs 4-5 hours; continuously-cultured un-mutant (CD59<+>) cells and mutant (CD59<->) cells can be obtained at the same time so that the efficiency of detecting CD59 gene mutation can be improved greatly. With the adoption of the method, the spontaneous mutation rate of the AL cells can be reduced and about 30 mutant cells exist in 105 survival cells and are reduced by two times as much as the spontaneous mutation after Panning, so that the experiment sensitivity of a mutation detection system is improved effectively.

A new and distinct variety of climbing shrub rose plant is provided. The new plant is a spontaneous mutation of unknown causation of the ‘Meiviolin’ variety (U.S. Plant Pat. No. 6,892). Attractive near white long-lasting very double blossoms having an ancient rose configuration are formed on a substantially continuous basis. Vigorous medium green semi-glossy foliage is formed that contrasts well with the near white blossom coloration. Good resistance to Black Spot and good tolerance to frost are displayed. The new variety is particularly well suited for providing attractive ornamentation in the landscape. It can be grown to advantage on a trellis, lamppost or gazebo.