176 results about "Mutation frequency" patented technology

The present invention provides compositions and improved methods for multi-site directed mutagenesis and DNA shuffling. The present compositions and methods provide increased mutation frequency and increased number of transformants which allow one to sequence only a few clones in order to identify the correct mutants and to obtain the desired mutant by screening large number of transformants in a short time. Moreover, the inclusion of FEN-1, PEF and optimized buffer and cycling conditions provided in the present invention should also facilitate random mutagenized library construction and the mutagenesis of large or difficult templates.

The invention discloses a method and device for detecting somatic cell SNP. The method comprises the steps of S1, comparing DNA sequencing data of somatic cells with reference genome data to obtain data of all SNP loci; S2, screening out SNP loci which occur on splicing loci and SNP loci which occur on exons and cause nonsynonymous mutation of amino acid to obtain a first SNP locus set; S3, removing SNP loci with the thousand-people mutation frequency higher than 5% in the first SNP locus set to obtain a second SNP locus set; S4, screening out SNP loci which are resources of somatic cell mutation from the second SNP locus set to obtain a third SNP locus set; S5, screening out SNP loci with the frequency of supporting sequences lower than 75% from the third SNP locus set to obtain SNP loci containing somatic cell SNP. By means of the method, somatic cell mutation can be detected through simple samples, and therefore the cost is lowered.

The invention relates to an intelligent tumor medication instruction system. The intelligent tumor medication instruction system is characterized by comprising a patient information module, a gene mutation detection module, a medication instruction database and a detection result reporting module, wherein the patient information module is used for recording the basic information of a patient; the gene mutation detection module is used for detecting the gene mutation information of the patient, such as a gene name, a mutation site, mutation conditions, mutation frequency and germline mutation and / or somatic cell mutation; the medication instruction database is used for performing medication instruction and query according to the clinical information of the patient, automatically matching and sequencing the clinical information and the medication instruction and query results, and sending an optimum medication instruction scheme suitable for the tumor patient to the detection result reporting module; a doctor can instruct the medication on the tumor patient according to the medication scheme displayed by the detection result reporting module. The intelligent tumor medication instruction system is widely applied to the diagnosis and medication instruction process of the tumor patient.

The invention relates to 86 types of particular mutation type atlases of SLC26A4 gene related to hearing loss in Chinese crowd, 27 types of relative hot-spot mutation type atlases and frequency information thereof, 13 types of hotspot mutation type atlases and frequency information thereof, and 2 types of hottest-spot mutation type atlases and frequency information thereof. 59 SLC26A4 gene mutation types are newly discovered in the Chinese crowd, wherein, 47 mutation types lead to the change of encoded protein amino acid of the SLC26A4 gene or influence genetic transcription and translation, 6 mutation types lead to base change rather than the change of amino acid, and 6 types are intron mutation types of the SLC26A4 gene. The discovery has a vital practical significance in developing an SLC26A4 gene diagnosing chip and a kit, which conform to hereditary features of the Chinese crowd suffering hearing loss.

The invention belongs to the field of medicines and biology, and relates to a blood plasma cfDNA detection technology-based noninvasive pancreas cancer polygene detection method and kit. The blood plasma cfDNA detection technology-based noninvasive pancreas cancer polygene detection method comprises the following steps: collecting blood, separating blood plasma, and purifying cfDNA; designing the panel of a pancreas cancer characteristic hotspot mutation region; and carrying out PCR primer amplification, constructing a library, and carrying out high-flux next generation sequencing and data analysis. The dynamic change of the mutation type and the mutation frequency of pancreas cancer characteristics in the blood plasma cfDNA is detected according to the treatment process and the pathogenesis development of every individual, so information is provided for pathogenesis monitoring and resistance drugs of the pancreas cancer.

The invention discloses a rice gene BADH2 site-directed knockout system and application thereof. The site-directed knockout system is a), b) or c) as follows: a) T-BADH2b and T-BADH2a are included, b) T-BADH2b is included, and c) T-BADH2a is included, wherein T-BADH2b and T-BADH2a are both specifically spliced transcriptional activation subsample effective factor nucleases of rice gene BAHD2; and the target sequences of the both are respectively 1589-site to 1640-site nucleotide sequence and 273-site to 324-site nucleotide sequence in the sequence 1 of the sequence table. Experiments prove that the mutation frequency of the rice gene BADH2 by singly using T-BADH2b is 26.9%, the mutation frequency of the rice gene BADH2 by singly using T-BADH2a is 9.2%, and at the same time the mutation frequency of the rice gene BADH2 in the resistance callus obtained by expression of T-BADH2b and T-BADH2a in the rice Nipponbare callus is 18.1%, wherein the occurrence frequency of fragment deletion is 5.1%. The invention provides an efficient breeding method for creating the fragrant rice genetic resources and culturing the excellent fragrant rice variety.

The present invention relates to a trigonometric function speed planning method applied to spline interpolation and divided into the pre-interpolation and the real-time spline interpolation. The pre-interpolation is characterized by carrying out the rapid pre-interpolation on a to-be-processed curve, collecting the acceleration / deceleration starting points and the information of the speed maximum / minimum value points on the processed curve, then calculating a sped equation of each acceleration / deceleration segment, and saving in an acceleration / deceleration array. A real-time spline interpolation stage is characterized by firstly calculating the real-time feed speed of a cutter according to the acceleration / deceleration beginning and end parameters and the speed equations in the acceleration / deceleration array, then adopting a two-order Taylor expansion to calculate the next real-time interpolation parameter real-timelyto carry out the real-time spline interpolation. The method of the present invention is simple to control, can guarantee the processing accuracy, also can realize the continuous change of the speed and the acceleration during a high speed processing process of a numerical control machine tool, and enables the mutation frequency of the jerk to be reduced, thereby relieving the overshoot generated by the high speed processing, and realizing the flexible acceleration / deceleration control of a servo shaft.

The invention discloses a chemical mutation breeding method for bougainvillea, and relates to the field of the vegetative propagation and chemical mutation breeding of xylophyta and vines. The method treats cottage pieces of bougainvillea with sodium azide (NaN3), adopts continuous cottage propagation to create a time and a space which are good for growth of leaf color mutants, and obtains new lines with stable heredity and ornamental value through generation selection, thereby achieving the purpose of chemical mutation breeding. By adopting the technical scheme in the invention, large quantities of female parent materials can be treated in one step; the base number of variants is relatively increases greatly; the mutation frequency is increased; the treatment cost is relatively low; and the breeding period can be shorten effectively. By utilizing the continuous cottage propagation manner, the growth of leaf color mutants is ensured to be competitive, which is helpful for overcoming the chimera formation, thereby obtaining stable and uniform variants. The chemical mutation breeding method can obtain high-frequency mutation and a wide spectrum of variation, and is helpful for the variety improvement of bougainvillea.

The invention discloses a mutation and screening method for salt-tolerant buckwheat mutants. The method comprises the following steps: treating and mutating buckwheat seeds by adopting EMS (Ethyl Methyl Sulfone), screening buckwheat mutants insensitive to ethylene by virtue of a direct precursor ACC (1-aminocyclopropane-1-carboxylic acid) obtained through ethylene synthesis, cultivating the buckwheat mutants into saline-alkali soil with the salinity of 0.4 to 0.6 percent, screening salt-tolerant plants, planting harvested buckwheat seeds into the saline-alkali soil with the salinity of 0.4 to 0.6 percent, and further screening the salt-tolerant buckwheat mutants with stable characters. According to the mutation and screening method for the salt-tolerant buckwheat mutants, the mutants are easily obtained by mutation with the EMS, DNAs are less damaged by point mutation, and meanwhile, screening with the direct precursor ACC in combination with ETH is combined to screen the salt-tolerant buckwheat mutants, so that the mutation frequency is high, the target is clear, the screening efficiency is high, and can reach 10 to 20 percent, the operation cost is low, and the obtained mutants have stable characters.

A method of improving DNA repair and reducing DNA damage and for reducing mutation frequency in skin for the purpose of reducing consequences of exposure to solar or ultraviolet radiation is disclosed. The methods comprise administering to the skin a composition containing deoxyribonucleosides in concentrations sufficient to enhance DNA repair or reduce mutation frequency in a vehicle capable of delivering effective amounts of deoxyribonucleosides to the necessary skin cells.