The invention belongs to the technical field of
plant breeding, and discloses a method for increasing the seedless grape crossed
embryo rescue
seedling rate. According to the method, before
abortion of seedless grape crossed embryos, physical measures of pinching and
girdling are taken to promote in-vivo development of ovules and embryos, the appropriate time for
embryo abortion rescue sampling of two Southern seedless grape hybridized combinations is confirmed, and content such as culture medium additives for culturing ovules and germinating embryos,
test tube seedling dual-
bud stem
inoculation subculturing and subculturing culture medium additives are confirmed. Due to the adoption of the method, the seedless grape crossed
embryo developing rate is high, the
embryo rescue seedlings are tidy and strong, the embryo developing rate is up to 41.7%, the
seedling rate is up to 18.3%, the subculturing culture seedling rate is up to 92%, the rate of strong and healthy seedlings is 81.8%, the
test tube seedling
transplanting survival rate is up to 97%, and the cardinal number of the crossed filial generation
embryo rescue seedlings is greatly increased, so that a relatively great number of seedless crossed generation
plant groups can be obtained, the seedless grape crossed embryo seedling rate in southern areas can be increased, and the method is high in
operability and easy to master.