The invention belongs to the technical field of plant breeding, and discloses a method for increasing the seedless grape crossed embryo rescue seedling rate. According to the method, before abortion of seedless grape crossed embryos, physical measures of pinching and girdling are taken to promote in-vivo development of ovules and embryos, the appropriate time for embryo abortion rescue sampling of two Southern seedless grape hybridized combinations is confirmed, and content such as culture medium additives for culturing ovules and germinating embryos, test tube seedling dual-bud stem inoculation subculturing and subculturing culture medium additives are confirmed. Due to the adoption of the method, the seedless grape crossed embryo developing rate is high, the embryo rescue seedlings are tidy and strong, the embryo developing rate is up to 41.7%, the seedling rate is up to 18.3%, the subculturing culture seedling rate is up to 92%, the rate of strong and healthy seedlings is 81.8%, the test tube seedling transplanting survival rate is up to 97%, and the cardinal number of the crossed filial generation embryo rescue seedlings is greatly increased, so that a relatively great number of seedless crossed generation plant groups can be obtained, the seedless grape crossed embryo seedling rate in southern areas can be increased, and the method is high in operability and easy to master.