Product
Patsnap Eureka
For R&D, Patsnap Eureka makes reading and utilizing patents & technical documents easy.
Patsnap Eureka AIR
Designed for self-driven R&D workflows. Generate viable solutions, solve complex R&D challenges, empower your innovation with AI.
Patsnap Eureka Materials
Designed for material experts only. Revolutionize your material R&D, from search, analyze, to developing new materials.
Feature
TechResearch
Generate reliable direction feasibility study reports for your R&D in just a few steps.
TechSeek
Discover and master advanced knowledge NOW. Basics, ideas, possibilities, all at once.
TechMind
As an expert in R&D Theories, TechMind can generates customized viable solutions instantly.
TechRisk
Analyze your overall solution with one click, know your potential R&D risks in advance.
TechMonitor
Get weekly tech updates, stay abreast of the latest tech innovations and key insights.
Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
106results about How to "Increase development rate" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
Bovine in vitro fertilization embryo culture medium and culture method
InactiveCN103898046BImprove developmental abilityIncrease development rateEmbryonic cellsCulture fluidEmbryo
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Prophase culture solution and anaphase culture solution for porcine early embryonic development
InactiveCN102093978AIncrease development rateBlastocysts are of good qualityEmbryonic cellsProphasePhases of clinical research
The invention discloses prophase culture solution (foregoing 60h) for porcine early embryonic (applicable to embryos before porcine blastocysts are hatched) development and anaphase culture solution (from 60h after the culture to a blastocyst hatching stage) for porcine early embryonic development. Calcium lactate, sodium pyruvate, beta-mercaptoethanol, dbcAMP.Na, nonessential amino acid and oestrus 7dth porcine serum are added on the basis of NCSU23 culture solution without CaC12. In addition, the difference between the prophase culture solution for porcine early embryonic development and the anaphase culture solution for porcine early embryonic development is that: the prophase culture solution for porcine early embryonic development does not contain glucose, and the anaphase culture solution for porcine early embryonic development contains the glucose; and particularly, when porcine early embryos are cultured, the prophase culture solution for porcine early embryonic development is used for culturing first, and the anaphase culture solution for porcine early embryonic development is used after 60h. Therefore, the developmental rate of the blastocysts and the quality of the blastocysts can be greatly improved.
Owner:SHANGHAI ACAD OF AGRI SCI
Method for improving in-vitro production efficiency of buffalo embryos
InactiveCN102140435AIncrease productivityPromote in vitro maturationEmbryonic cellsEmbryo transplantationBiology
The invention relates to a method for improving in-vitro production efficiency of buffalo embryos, comprising the following steps of: respectively adding 10 muL / mL of insulin-transferrin-selenium (ITS) and 20 muL / mL of insulin-transferrin-selenium (ITS) to an oocyte maturing solution and a culture solution, and then carrying out in-vitro maturation and in-vitro culture. The method indicates that the in-vitro maturation rate of buffalo oocyte is effectively improved by adding 10 muL / mL ITS to the maturing solution, and the exhaustion ratio of a first polar body is up to 65.61%; and adding 20 muL / mL of ITS to the embryo culture solution is beneficial to the growth of early embryos of a fertilized buffalo, and the growing rate of a blastaea is up to 29.93%. the method has the advantages of improving the in-vitro maturation rate of the buffalo oocyte and the growing rate of the blastaea, thereby improving the in-vitro production efficiency of the buffalo embryos and providing a large number of high-quality embryos for embryo transplantation.
Owner:GUANGXI UNIV
Mammal embryo in-vitro culture device and culture method thereof
InactiveCN102876573AEasy to operateEasy to useTissue/virus culture apparatusEmbryonic cellsCulture fluidCarbon dioxide
Owner:HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
Method for improving cultivation efficiency of bovine in vitro fertilization embryos
ActiveCN107142239AImprove developmental abilityIncrease development rateCulture processCell culture active agentsCulture fluidSodium pyruvate
The invention relates to a method for improving the cultivation efficiency of bovine in vitro fertilization embryos. In particular, the invention relates to bovine in vitro fertilization embryo cultivation liquid. The cultivation liquid contains NaCl, KCl, NaHCO3, MgCl2, KH2PO3, sodium pyruvate, glucose, calcium galactonate, fetal calf serum, L-glutamine, necessary amino acid, unnecessary amino acid, glutathione and water. The invention also relates to a method for improving the cultivation efficiency of the bovine in vitro fertilization embryos. The method comprises the following steps: putting the bovine in vitro fertilization embryos into the bovine in the vitro fertilization embryo cultivation liquid, and performing embryo in vitro cultivation under the conditions of 38.5 DEG C, 0.5 percent CO2 and 100 percent humidity. The method provided by the invention and the used cultivation liquid present excellent technical effects as stated in the specification.
Owner:无锡博裕力牧生物科技有限公司
Bovine oocyte in vitro maturation culture solution and culture method
ActiveCN108103011AImprove developmental abilityImprove ripening qualityCulture processCell culture active agentsBovine oocyteCulture fluid
The invention provides a bovine oocyte in vitro maturation culture solution and a culture method. The maturation culture solution consists of 10% of fetal calf serum and 1% of ITS-G, as well as 0.075IU / mL of HMG, 1[mu]g / mL of 17[beta]-estradiol, 10ng / mL of EGF, 2.2mg / mL of bFGF and 50ng / mL of CXCL12. The culture solution, when applied to bovine oocyte in vitro maturation culture, can improve maturation quality of bovine oocyte in vitro culture, a development rate of in vitro fertilized embryos as well as a development rate of somatic cell nuclear transplantation embryos and blastocyst quality.
Owner:NORTHWEST A & F UNIV
Method for bovine in-vitro fertilized embryo culture and used culture solution
ActiveCN107034173AImprove developmental abilityIncrease development rateCulture processCell culture active agentsCulture fluidEmbryo
The invention relates to a method for bovine in-vitro fertilized embryo culture and a used culture solution. Particularly, on one hand, the invention relates to a method for culturing a bovine in-vitro fertilized embryo. The method comprises the following steps of putting the bovine in-vitro fertilized embryo into a bovine in-vitro fertilized embryo culture solution and carrying out embryo in-vitro culture under the conditions that the temperature is 38.5 DEG C, the content of CO2 is 0.5% and the humidity is 100%. The invention further relates to a bovine in-vitro fertilized embryo culture solution. The culture solution comprises NaCl, KCl, NaHCO3, MgCl2, KH2PO3, sodium pyruvate, glucose, calcium galactonate, fetal calf serum, L-glutamine, an essential amino-acid, a nonessential amino acid, glutathione and water. The method and the used culture solution have excellent technical effects in the specification, for example, the method has a higher blastocyst hatching rate when the bovine in-vitro fertilized embryo is cultured.
Owner:天津力牧生物科技有限公司 +1
A method for processing bovine somatic cell cloned embryos based on somatic cell nuclear transfer
InactiveCN102296090AEfficient productionIncrease development rateFermentationGenetic engineeringNuclear transferOocyte
The invention discloses a method for processing bovine somatic cell cloned embryos constructed on the basis of somatic cell nuclear transplantation, which comprises the following steps of: injecting bovine somatic cells to be transplanted into denucleated oocyte to carry out electrofusion; and an hour later after the electrofusion is completed, selecting the successfully fused bovine somatic cellcloned embryos and processing the bovine somatic cell cloned embryos for 12 hours by 1muM of Oxamflatin. By the method, the developmental rate and the quality of the bovine somatic cell cloned embryos can be obviously improved and the bovine somatic cell cloned embryos can be efficiently produced in vitro.
Owner:NORTHWEST A & F UNIV
Vector, cell and method for improving bovine cloning efficiency on the basis of histone methylation modifying level
ActiveCN105524940AImprove bovine cloning efficiencyImprove cloning efficiencyFermentationVector-based foreign material introductionHistone methylationOperon
The present invention discloses a vector, a cell and a method for improving bovine cloning efficiency on the basis of histone methylation modifying level. The vector includes a repressor expression vector and a repressor-operon-containing expression vector, and in the transfection, the repressor expression vector and the repressor-operon-containing expression vector are cotransfected into donor cells; and bovine KDM4B or bovine KDM4C gene is inserted into the repressor-operon-containing expression vector. Positive transfected cells as donor cells are injected into enucleated oocytes for electro-fusion, and successfully-fused bovine somatic cell cloned embryos are selected for culturing for 36h for induced expression of the bovine KDM4B and bovine KDM4C. Histone H3K9me3 epigenetic modifying level of the somatic cell cloned embryos in zygotic activation period can be effectively reduced, the development and quality of post-bovine somatic cell cloned blastocysts can be significantly improved, efficient and in-vitro production of the bovine somatic cell cloned embryos can be achieved, and birth rate of cloned cattle after embryo transplant can be significantly increased.
Owner:NORTHWEST A & F UNIV +1
In vitro fertilization culture dish and in vitro embryo fertilization method
ActiveCN101451104AIncrease development rateGood qualityBioreactor/fermenter combinationsBiological substance pretreatmentsBiologySperm
The invention discloses an in vitro fertilization culture dish, which comprises a dish body (1), wherein a first accommodating area (11) for placing ovum and a second accommodating area (12) for placing sperms are arranged inside the dish body (1) and communicated with each other through at least one channel (13) which is tubular; and the top of the channel (13) is provided with a vent hole (15). Moreover, the invention also discloses an in vitro embryo fertilization method for utilizing the culture dish. The in vitro fertilization culture dish and the method can effectively guarantee that only high-quality sperms with enough vigor can reach the ovum and are possible to be combined with the ovum, so as to improve the developmental rate of fertilized embryos and the success rate of embryo transplantation and finally guarantee that bred offspring has superior quality.
Owner:曾勇 +1
Method for improving moveability of sperm in frozen bull semen and in vitro fertilization rate
The invention provides a method for improving the moveability of sperm in frozen bull semen and the in vitro fertilization rate. The method is characterized in that when SP-TALP washing liquid is used for washing at a prior disposal stage of the frozen bull semen, or when PHE (phenylalanine) capacitation liquid is used for capacitation disposal at a capacitation disposal stage of the semen before fertilization, or when fertilization drips are prepared at an in vitro fertilization stage, glutathione and caffeine of which the purities are both higher than 98% are added; in the fertilization process of each piece of frozen bull semen, in the SP-TALP washing liquid containing bull semen or in the mixed liquid of IVF-TALP seminal fluid containing the bull semen and the PHE capacitation liquid or in the mixed liquid of the fertilization drips containing oocytes and the bull semen, the final concentration of the glutathione and the caffeine is 5mmol / L. According to the method disclosed by the invention, two substances are jointed to be respectively applied to three stages in the fertilization process of the frozen bull semen, the moveability of the sperm is greatly improved, and the fertilization rate of the sperm is improved.
Owner:武汉市畜牧兽医科学研究所
Freezing liquid for preserving embryo, preparation method and application thereof
InactiveCN102771472AHelps to escapePlay a protective effectDead animal preservationSucroseBovine serum albumin
The invention discloses a freezing liquid for preserving embryo, especially a freezing liquid for preserving sampled embryo. The freezing liquid comprises ethylene glycol and sucrose, and is characterized by comprising 0.4-5g of arabinogalactan, 75-85mL of ethylene glycol, 30-40g of sucrose, and 910-930mL of PBS buffer solution containing 0.0021 g / mL bovine serum albumin (BSA). A preparation method of the freezing liquid comprises the steps of dissolving ethylene glycol, sucrose and arabinogalactan in the BSA-containing PBS buffer solution. The freezing liquid fully utilizes synergic effects of the four components that ethylene glycol, sucrose, arabinogalactan and bovine serum albumin are coordinated and supported one another, and has higher development rate of thawed sampled embryo in comparison with conventional ethylene glycol freezing liquid.
Owner:石家庄天泉良种奶牛有限公司
Application of decidua NK cells and decidua NK cell exosomes from cell subsets in preparing drugs and auxiliary therapeutic agents for treating diseases related to infertility
ActiveCN110721196AIncrease development rateImprove implantation rateCell dissociation methodsMammal material medical ingredientsDiseaseEndometrium thickness
The invention provides application of decidua NK cells and decidua NK cell exosomes from cell subsets in preparing drugs and auxiliary therapeutic agents for treating diseases related to infertility.Experiments prove that the exosomes treat endometrium growth disorders by promoting endometrial thickness increasing, improving endometrial cell activity, reducing endometrial cell damage, promoting VEGF expression, maintaining stem features of endometrium matrix cells and stimulating proliferation, so that the pregnancy success rate of endometrium damage model mice is increased to 50-70% from 20%; and the exosomes treat diseases related to maternal-fetal immunologic tolerance disorders by playing a role of immunologic tolerance, reducing a spontaneous abortion rate and improving a help T lymphocyte level. Besides, the exosomes can effectively increase the developmental rate of zygotes fertilized no matter in vitro or in vivo in a multiple mode, meanwhile, an in-vitro fertilization rate, an implantation rate of implantation and a birth rate are also increased, and positive assisting effects on infertility treatment are achieved.
Owner:PHARCHOICE THERAPEUTICS INC
Method for improving in-vitro developmental capacity of ovine oocytes
ActiveCN104342400AIncrease development rateScientific and reasonable technical routeGerm cellsBiologyChromosome segregation
The invention provides a method for improving the in-vitro developmental capacity of ovine oocytes. The method comprises the following steps: carrying out in vitro maturation and in vitro fertilization on inferior BCB-oocytes obtained by chromosome segregation; culturing by 200nMol / L of culture solution containing histone deacetylase inhibitor trichostatin A (TSA) for 10 hours, and continuously culturing by a culture solution without TSA, calculating the cleavage rate after culturing for 48 hours, and calculating the blastocyst rate after culturing for 7 days; and washing the BCB+ oocytes obtained by chromosome segregation by mature culture solution for 2-3 times, and culturing for 24 hours to directly utilize. The method has an important practical value on in vitro utilization of ovine oocytes.
Owner:新疆维吾尔自治区畜牧科学院中国-澳大利亚绵羊育种研究中心
Magnetic cell and magnetic memory
InactiveUS7042758B2Increase development rateReduce volatilityMagnetic-field-controlled resistorsSolid-state devicesDiffusionMagnetic memory
It is possible to provide a magnetic cell having a high developing rate of MR characteristics and a reduced fluctuation without causing element falling-down and a magnetic memory having the same. A magnetic cell includes: a lower electrode; an electrically conductive pillar formed on the lower electrode; a magnetoresistance effect film having at least two ferromagnetic layers formed on the electrically conductive pillar and an intermediate layer provided between the ferromagnetic layers; an upper electrode formed on the magnetoresistance effect film; a support layer formed from at least one metal directly on a side face of the electrically conductive pillar or via an insulating layer; and a current diffusion preventing layer provided between the support layer and the lower electrode, wherein a height of the electrically conductive pillar, a thickness of the current diffusion preventing layer, and a thickness of the support layer satisfy relationships of h>t1+t2>3030+L×hwhere h represents the height of the electrically conductive pillar, t1 represents the thickness of the current diffusion preventing layer, t2 represents the thickness of the support layer, and L (nm) represents a length of a short side of the electrically conductive pillar.
Owner:KK TOSHIBA
Epinephelus septemfasciatus parent fish reproduction and maturing bait and preparation method thereof
InactiveCN103283962AGuarantee artificial breeding productionNutritional balanceAnimal feeding stuffBroodstockAnimal science
The invention provides an epinephelus septemfasciatus parent fish reproduction and maturing bait and a preparation method thereof. The epinephelus septemfasciatus parent fish reproduction and maturing bait is formed by following components in percentage: 30% of artificially-matched powder feed (main components comprise fish meal, starch, soybean meal powder, peanut bran powder and the like), 10% of opossum shrimp powder, 10% of oyster / mya arenaria linnaens meat (fresh and alive products or chilled products), 20% of sardinella zunasi (chilled products), 3.2% of concentrated micro-mud marimo, 10% of loligo japonica (fresh and alive products or chilled products), 10% of clam worms (fresh and alive products or chilled products), 5% of brine shrimp powder, 0.5% of compound vitamin, 0.4% of astragalus membranaceus powder, 0.4% of lecithin powder and 0.5% of immune polysaccharides. The bait disclosed by the invention not only can guarantee the nutritional requirement of parent fish, but also can effectively improve the developmental rate of parent fish sexual glands, accelerate the sexual glands to synchronously develop and improve the egg laying amount and the ovum quality of female parent fish, so as to lay a foundation for large-scale larval rearing of epinephelus septemfasciatus.
Owner:MARICULTURE INST OF SHANDONG PROVINCE
Modified culture method for promoting cloned mouse embryonic development
InactiveCN101798567AIncrease development rateImprove the efficiency of building a departmentFused cellsAnimal husbandryBiotechnologyNuclear transfer
The invention relates to a modified culture method for promoting cloned mouse embryonic development. The method is a continuous culture method-D culture method. The invention also relates to two culture systems capable of increasing the developmental rate of cloned mouse embryo, a method of using the culture systems for obtaining cloning animal and a method of using the culture systems for obtaining embryonic stem cells. By using the culture method of the invention, the developmental rate of cloned mouse embryo and the establishment efficiency of nuclear transfer of embryonic stem cells are increased, thus paving a new way for using clone technology in the production and facilitating the further development of animal cloning technology.
Owner:赵巍
Methods for in vitro oocyte maturation
InactiveUS20130034906A1High rate of developmental competenceImprove metabolic activityHybrid cell preparationCell culture supports/coatingMammalReproductive technology
Owner:RGT UNIV OF MICHIGAN
Special clamping tool for robot for live replacement of strain single insulator of ultrahigh-voltage line
ActiveCN111276899ARealize live automatic replacementImprove reliabilityApparatus for overhead lines/cablesUltra high voltageClassical mechanics
The utility model discloses a special clamping tool for a robot for the live replacement of a tension single insulator of an ultrahigh-voltage line. Each of a front clamp (3) and a rear clamp (1) comprises a main body (101) and an upper cover (102), wherein a collision block locking mechanism is arranged between the upper cover (102) and the main body (101); a motor locking mechanism (9) is arranged at the threaded connection part of the upper cover (102) and the main body (101); a motor (901) of the motor locking mechanism (9) is electrically connected with a robot controller; an adjusting lead screw (5) and a pulling plate frame assembly (2) are respectively and rigidly connected to two ends of the front clamp (3) and the rear clamp (1) to form an insulator tightening device; the end ofone side of the front clamp (3) and the end of one side of the rear clamp (1) are fixedly connected with a robot arm (7). The clamping tool has the advantages that the clamping tool is suitable for arobot to realize automatic replacement of the low-value single insulator at high altitude, the structure is simple, and automatic operation is easy to realize.
Owner:STATE GRID HUBEI ELECTRIC POWER CO LTD MAINTENANCE CO +1
Vitrification refrigerating liquid and refrigerating method thereof
InactiveCN108244100AImprove survival rateIncrease cleavage rateDead animal preservationVitrificationMaturation oocyte
The invention belongs to the field of assisted reproduction and particularly relates to vitrification refrigerating liquid. According to the vitrification refrigerating liquid provided by the invention, taxol is added to serve as a cryoprotectant and the vitrification refrigerating liquid obtained with reference to the taxol adding mode is treated, so that the development rate of in vitro fertilized blastocyst after the oocyte is thawed can be increased. The taxol serving as an additive in the refrigerating liquid can achieve the effects of reducing freezing injury of the mature oocytes and improving the development capability of the embryo after fertilization. The optimal concentration of the adding mode is obtained by researching the adding concentration of the taxol. The vitrification refrigerating liquid provided by the invention can greatly increase recovery rate after the frozen mature oocytes are thawed and the development rate of the blastula and achieves the protective effecton the cell ultrastructure.
Owner:成都艾伟孚生物科技有限公司
Zuogui pill serum containing active ingredients of Zuogui pill serum metabolic substance groups and preparation method and application of Zuogui pill serum
InactiveCN106434533AIncrease contentAchieve high enrichmentCulture processMammal material medical ingredientsSerum igeMedicine
The invention discloses Zuogui pill serum containing active ingredients of Zuogui pill serum metabolic substance groups and a preparation method and application of the Zuogui pill serum. The preparation method includes the steps of (1), mixing medicinal materials for preparing Zuogui pills according to a formulation dosage for preparing the Zuogui pills and performing water extraction so as to obtain water extract; (2), administrating the water extract in animals at a dosage of 10-20 g kg<-1> d<-1> for 7-10 days continuously, once to twice per day, collecting blood after administration, and collecting serum after blood centrifugation so as to obtain the Zuogui pill serum. The Zuogui pill serum is highly rich in glycoside active ingredients and is capable of remarkably increasing an inhibited embryo development rate under intervention of high glucose to promote embryo development.
Owner:SHANXI UNIV OF CHINESE MEDICINE
Method for improving compatibility and development rate of interracial nuclear transfer embryo nucleoplasm
InactiveCN102382857AGood compatibilityIncrease development rateEmbryonic cellsGenetic engineeringEmbryoPerivitelline space
The invention provides a method for improving compatibility and development rate of interracial nuclear transfer embryo nucleoplasm. The method includes the steps: (a) a cell nucleus of a nuclear donor cell is moved into a perivitelline space of an enucleated oocyte of a non-human mammal and integrated by means of electric stimulation, so that a reconstructed ovum is formed, (b) a human oocyte is moved into the reconstructed ovum in the step (a), and (c) the reconstructed ovum in the step (b) is electrically stimulated so as to form a reconstructed ovum composed of the cell nucleus of a human cell, the enucleated oocyte of the non-human mammal and the human oocyte. The embryo development rate can be increased remarkably by the method.
Owner:SHANGHAI TRANSGENIC RES CENT
Low-density high-strength petroleum ceramsite proppant taking tuff as main raw material, and preparation method thereof
ActiveCN104232074AImprove development rateLower firing temperatureFluid removalDrilling compositionPelletizingPowder
The invention discloses a low-density high-strength petroleum ceramsite proppant taking tuff as a main raw material, and a preparation method thereof. The ceramsite preparation materials is prepared from the following components in percentage by weight: 40%-60% of tuff mineral powder, 30%-45% of bauxite and 5%-20% of magma powder, wherein the total weight percent is 100%, and the volume of modified polyphenyl particles accounts for 30% of the total volume of the materials. The method comprises the following steps: weighing a certain amount of tuff, bauxite and a small amount of magma, blending and grinding, adding a certain amount of polyphenyl particles in a ceramsite pelletizer, pelletizing through a ceramsite making machine to make balls, drying the balls, screening the balls according to grain size, and roasting the qualified balls at the temperature of 1100-1190 DEG C for 20-40 minutes, so as to obtain the fired low-density high-strength proppant. The low-density high-strength petroleum ceramsite proppant utilizes the characteristics of high silicon content of the tuff and the magma, so that the ceramsite firing temperature is reduced, the ceramsite strength is improved at the same time, and the production cost is lowered.
Owner:XINYANG CITY SHANGTIANTI YIHE MINERAL PROD RESOURCES DEV
In vitro culture liquid and culture method for pig parthenogenetic activation embryo
ActiveCN106834216AOvercoming Barriers to Developmental BlockingEnhanced inhibitory effectCulture processCell culture active agentsMicrobiologySolvent
The present invention discloses an in vitro culture liquid (mPZM-3) and a culture method for a pig parthenogenetic activation embryo, and belongs to the field of in vitro animal embryo culture. The in vitro culture liquid (mPZM-3) comprises raw materials such as a mPZM-3 base culture liquid, calcium lactate pentahydrate and sodium pyruvate, wherein the concentration of the calcium lactate pentahydrate in the in vitro culture liquid is 0.606 g / L, the concentration of the sodium pyruvate is 0.022 g / L, the solvent used by the in vitro culture liquid is hydrogen-rich water, and the H2 content is 1.2-1.6 ppm. The culture method for the pig parthenogenetic activation embryo by using the in vitro culture liquid comprises: washing a pig parthenogenetic activation embryo by using an in vitro culture liquid containing 5 [mu]mol / L cytochalasin B, treating for 3-5 h in the in vitro culture liquid containing 5 [mu]mol / L cytochalasin B, washing with a cytochalasin B-free in vitro culture liquid, and culturing for 168 h in the cytochalasin B-free in vitro culture liquid until the embryo develops into a blastula. According to the present invention, with the in vitro culture liquid and the culture method, the energy and the development environment can be well provided for the ig parthenogenetic activation embryo so as to improve the culture quality and the culture effect of the embryo.
Owner:SHANGHAI ACAD OF AGRI SCI
Amphoteric polymer resins that increase the rate of sizing development
InactiveCN1610708AIncrease development rateReduce dosageWater-repelling agents additionPaper/cardboardOrganic acidPolymer resin
Owner:SOLENIS TECH CAYMAN
Vector and method for increasing cattle cloning efficiency based on histone methylation level modification
PendingCN108410894AEfficient productionAttenuated levels of epigenetic modificationMicroinjection basedOxidoreductasesHistone methylationEmbryo transfer
The invention discloses a vector and method for increasing cattle cloning efficiency based on histone methylation level modification. The vector comprises the in-vitro transcription template vector containing a cattle KDM4E gene. A large amount of mRNA of the cattle KDM4E gene can be obtained by subjecting the vector to in-vitro transcription reaction, and the mRNA is injected to a successfully fused and activated bovine somatic cell cloning embryo to allow the embryo to overexpress cattle KDM4E protein. The method has the advantages that histone H3K9me3 and H3K9me2 epigenetic modification level of the somatic cell cloning embryo during zygotic activation can be effectively lowered, so that the development rate and quality of later bovine somatic cell cloning embryo can be increased evidently, the method can be used for efficiently producing the bovine somatic cell cloning embryo in vitro, and the birth rate of cloning cattle after embryo transplanting is increased evidently.
Owner:NORTHWEST A & F UNIV
Method for processing bovine somatic cell cloned embryos constructed on basis of somatic cell nuclear transplantation
InactiveCN102296090BEfficient productionIncrease development rateFermentationGenetic engineeringBlastulaSomatic cell
The invention discloses a method for processing bovine somatic cell cloned embryos constructed on the basis of somatic cell nuclear transplantation, which comprises the following steps of: injecting bovine somatic cells to be transplanted into denucleated oocyte to carry out electrofusion; and an hour later after the electrofusion is completed, selecting the successfully fused bovine somatic cellcloned embryos and processing the bovine somatic cell cloned embryos for 12 hours by 1muM of Oxamflatin. By the method, the developmental rate and the quality of the bovine somatic cell cloned embryos can be obviously improved and the bovine somatic cell cloned embryos can be efficiently produced in vitro.
Owner:NORTHWEST A & F UNIV
Nuclear transfer embryo formation method
InactiveUS20040019924A1Confirm comparative in vitro development potentialIncrease chanceTissue cultureFermentationAnimal scienceMeiosis
A nuclear transfer embryo is formed by destabilizing microtubules of an oocyte, whereby essentially all endogenous chromatin collects at a second polar body during meiosis of an oocyte. The oocyte is fused with the nucleus of a donor somatic cell of the same species of said oocyte prior to cessation of extrusion of the second polar body from the oocyte, thereby forming the nuclear transfer embryo. In one embodiment, the nuclear transfer embryo is employed to impregnate an animal, such as a mammal. In another embodiment, the donor nucleus is transgenic.
Owner:TRUSTEES OF TUFTS COLLEGE
Method for improving cattle cloning efficiency by micro ribonucleic acid
ActiveCN106086080AIncrease development rateQuality improvementEmbryonic cellsGenetic engineeringEmbryoBiological activation
The invention discloses a method for improving cattle cloning efficiency by micro ribonucleic acid. bta-miR-125b mimic is added into electrical fusion solution of somatic cell nucleus transfer and can be effectively led into an embryo, the histone H3K9me3 epigenetic modification level of a somatic cell cloning embryo in a zygotic activation period can be effectively weakened while secondary damage of transfection is avoided, and the developmental rate and the quality of a cattle somatic cell cloning blastaea can be obviously improved.
Owner:NORTHWEST A & F UNIV
Monkey-origin embryonic stem cells
InactiveUS20030157710A1Reduce the impactIncrease development rateMicrobiological testing/measurementDrug screeningDiseasePrimate
A method for producing a monkey-derived embryonic stem cell comprising the steps of carrying out fertilization by insemination by in vitro fertilization or intracytoplasmic sperm injection using a monkey ovum and monkey sperms, thereby giving a fertilized ovum, allowing the fertilized ovum to differentiate into a blastocyst by in vitro culture, and establishing an ES cell line using the blastocyst; the monkey ES cell obtained by the method, a method for screening a reagent for specific differentiation into cell or tissue by using the ES cell; and a differentiated cell or differentiated tissue each differentiated from the ES cell. According to the present invention, applications of the embryonic stem cells to embryological studies clinical applications, experimental models, and the like on primates, studies of diseases, are expected.
Owner:MITSUBISHI TANABE PHARMA CORP
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com