47 results about "Endometrial cell" patented technology

The present invention relates to markers of endometriosis which are differentially expressed in the endometrial cells of females with endometriosis compared to endometriosis-free females. The invention also relates to methods for determining likelihood of endometriosis in female subjects, to methods for grading endometriosis in females suffering from endometriosis and to methods for treating this disease. The invention is also concerned with polynucleotides, probes, primers and kits useful for reducing into practice the above-mentioned methods which are more rapid, non invasive, much less complicated and much less costly than laparoscopy.

The present invention provides biomarkers for the diagnosis and prognosis of endometriosis. Generally, the methods of this invention find use in diagnosing or for providing a prognosis for endometriosis by detecting the expression levels of biomarkers, which are differentially expressed (up- or down-regulated) in endometrial cells from a patient with endometriosis. Similarly, these markers can be used to diagnose reduced fertility in a patient with endometriosis or to provide a prognosis for a fertility trial in a patient suffering from endometriosis. The present invention also provides methods of identifying a compound for treating or preventing endometriosis. Finally, the present invention provides kits for the diagnosis or prognosis of endometriosis.

The present invention relates to markers of endometriosis which are differentially expressed in the endometrial cells of females with endometriosis compared to endometriosis-free females. The invention also relates to methods for determining likelihood of endometriosis in female subjects, to methods for grading endometriosis in females suffering from endometriosis and to methods for treating this disease. The invention is also concerned with polynucleotides, probes, primers and kits useful for reducing into practice the above-mentioned methods which are more rapid, non invasive, much less complicated and much less costly than laparoscopy.

Disclosed herein is a method for culturing and proliferating hematopoietic stem cells or progenitor cells. The method comprises co-culturing the hematopoietic stem cells or the progenitor cells with human endometrial stromal cells so as to promote the expansion of hematopoietic stem cell-derived CD34+ cells or the growth of the progenitor cells. According to the disclosed method, unlike the prior methods of culturing the hematopoietic stem cells with the addition of cytokines, growth factors or hormones, the hematopoietic stem cells or the progenitor cells are co-cultured with the endometrial stromal cells or epithelial cells so that the number of CD34+-enriched hematopoietic stem cells or progenitor cells is increased by factors secreted from the endometrial stromal cells or epithelial cells. Accordingly, it is possible to eliminate the addition of growth factors, such as cytokines, and hormones, which cause problems in transplantation of hematopoietic stem cells or progenitor cells and thus safe hematopoietic stem cells or progenitor cells can be obtained in large amounts.

The invention provides application of decidua NK cells and decidua NK cell exosomes from cell subsets in preparing drugs and auxiliary therapeutic agents for treating diseases related to infertility.Experiments prove that the exosomes treat endometrium growth disorders by promoting endometrial thickness increasing, improving endometrial cell activity, reducing endometrial cell damage, promoting VEGF expression, maintaining stem features of endometrium matrix cells and stimulating proliferation, so that the pregnancy success rate of endometrium damage model mice is increased to 50-70% from 20%; and the exosomes treat diseases related to maternal-fetal immunologic tolerance disorders by playing a role of immunologic tolerance, reducing a spontaneous abortion rate and improving a help T lymphocyte level. Besides, the exosomes can effectively increase the developmental rate of zygotes fertilized no matter in vitro or in vivo in a multiple mode, meanwhile, an in-vitro fertilization rate, an implantation rate of implantation and a birth rate are also increased, and positive assisting effects on infertility treatment are achieved.

The present invention relates to newly identified nucleic acid molecules and polypeptides present in normal and neoplastic endometrial cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of the polypeptides of the invention. The invention also relates to compositions containing the nucleic acid molecules, polypeptides, antibodies, agonists and antagonists of the invention and methods for the use of these compositions. These uses include identifying, diagnosing, monitoring, staging, imaging and treating endometrial cancer and non-cancerous disease states in endometrial, identifying endometrial tissue, monitoring and identifying and / or designing agonists and antagonists of polypeptides of the invention. The uses also include gene therapy, production of transgenic animals and cells, and production of engineered endometrial tissue for treatment and research.

Method for gentle separation of viable sporadic cells from body fluids such as blood, from malignant effusions, bronchoalveolar lavage fluid, peritoneal lavage fluid and amniotic fluid, based on a filter membrane which is in an intimate contact with an absorbent material. Using the present method it is possible to isolate for example circulating and disseminated tumor cells, endometrial cells and circulating trophoblast cells, allowing subsequent detection, quantification, characterization and especially culturing of said cells. An apparatus for carrying out the method is further disclosed.

The invention provides a method for separating and purifying endometrial stromal cell and proceeding with large scale external culture, which improves the external purifying ratio and the breeding ability of endometrial stromal cell, adjusts the proliferative stage of endometrium and uterus physiology by treating adenotropic enthusiasm hormone and adenotropic ovulation hormone for the experimental animal, purifies the endometrial stromal cell with centrifuge method, establishes an external culture system with estrogen and gestagen in the cell purification and breeding, improves the breeding ability of the endometrial stromal cell, achieves a large scale breeding purpose and provides the abundant seed cell for the construction of the organizing engineer uterine tissue.

The invention relates to the technical field of medicines, and provides applications of uterine cavity fluid-derived exosome in preparation of drugs and adjuvant therapeutic agents for treating infertility related diseases. Through the verification of experiments, exosome realizes the treatment or relieving of endometrial growth disorders by promoting the increasing of the thickness of endometrium, enhancing the activity of endometrial cells, reducing the damages to intimal cells, promoting VEGF expression, maintaining the dryness of endometrial stromal cells and stimulating proliferation; andthrough the realizing of the treating or relieving of maternal-fetal immune tolerance disorder-related diseases by achieving immune tolerance effects, treating spontaneous abortion and enhancing T helper lymphocyte levels, a novel theoretical basis can be provided for infertility treatment. In addition, for both fertilized eggs being fertilized in vivo or fertilized in vitro, the exosome can effectively enhance developmental rates in multiples; and implantation rates and farrowing rates of in vitro fertilization transplantation can be increased as well, so that positive auxiliary effects on infertility treatment can be achieved.

The invention discloses a preparation method and application of a composite decellularized amniotic membrane of endometrial stem cells in the technical field related to physiological health care, including preparation of complete amniotic membrane tissue, preparation of a translucent amniotic membrane, immersion disinfection, decellularization treatment, preparation of a stem cell amniotic membrane culture system, preparation and cultivation of endometrial stem cell composite decellularized amniotic membrane and other processes as well as cryopreservation, thawing and application. The endometrial stem cell composite decellularized amniotic membrane prepared by the method is applied to the preparation of endometrial repair materials, is transplanted into the endometrial injury site for repair of endometrial damage and increase the thickness of the endometrium; the decellularized amniotic membrane completely removes cells and nucleic acids, and does not produce rejection reactions in thebody; through combination of menstrual blood stem cells or endometrium stem cells with the decellularized amniotic membrane, the cells are homologous to endometrial cells, and the repair effect is good; the use of hormones in repair is completely excluded to avoid interference with the internal secretion of a human body.

The invention provides an application of decidua NK cells and cell subsets thereof in preparation of medicines for treating infertility related diseases. Through experimental verification, according to the decidua NK cell and the cell subset thereof, endometrial growth disorder diseases are treated by promoting endometrial thickness increase, enhancing endometrial cell viability, reducing endometrial cell damage, promoting VEGF expression, maintaining endometrial stromal cell dryness and stimulating proliferation; the pregnancy success rate of endometrial injury model mice is increased from 30% to 60-70%; by exerting the immune tolerance effect, reducing the spontaneous abortion rate and improving the T helper lymphocyte level to treat maternal fetal immune tolerance disorder related diseases, a new way is provided for treatment of infertility.

The invention discloses a traditional Chinese medicine combination for the treatment of endometriosis uterine, pertaining to the field of traditional Chinese medicines. The traditional Chinese medicine combination comprises yam, dwarf lilyturf turber, burred tuber, radix salivae miltiorrhizae, root of baikal skullcap, peach kernel, radix paeoniae rubra, atractylodes macrocephala, pangolin and safflower. The traditional Chinese medicine combination utilizes methods of dispelling cold by warming the meridians, vigorating qi and replenishing the blood, dissipating phlegm and eliminating blood stasis as well as softening and resolving hard masses for regulating the functions of entrails, promoting the death of endometrial cells, controlling the growth and proliferation of endometrium in other parts of human body so as to ensure obvious relief and disappearance of menalgia, callosum falling back pain and pelvic adhesions and disappearance of cystic mass, thereby having good efficacies of dispersing blood stasis and dredging entrails and strengthening the body resistance, dredging the channels and collaterals and driving out evil spirits, softening and resolving hard masses and detumescence as well as nourishing yin and tonifying the kidney and relieving pain.

The invention discloses an in-situ primary endometrial cancer tumor model preparation method, and belongs to the field of tumor animal models. The method comprises the following steps: culturing mouse endometrial cells into organoids by using a specific culture medium, performing gene editing on the organoids, and injecting the organoids back to the mouse endometrium so as to enable the organoids to develop into tumors. The tumor model constructed by the invention has the characteristics of primary, in-situ and definite genotype, and compared with a genetic engineering tumor animal model, the time consumption is short, and the tumor formation rate is high; compared with a transplanted tumor animal model, the model has an in-vivo microenvironment for tumor generation and development, is closer to the most real state of endometrial cancer, and has a good application prospect.

The invention discloses application of cyanidin-3-O-glucoside in preparation of a medicine for treating and / or preventing an endometrial hyperplasia disease. The cyanidin-3-O-glucoside is found to becapable of improving endometrial hyperplasia and endometrial hyperproliferation caused by Cd exposure, and the cyanidin-3-O-glucoside intervenes in the process of protecting growth and development ofendometrial cells by maintaining the estrogen effect level, so that the uterus can normally grow, embryo implantation is ensured, and meanwhile, the cyanidin-3-O-glucoside has a protection effect on the endometrium. Therefore, the cyanidin-3-O-glucoside has a good application prospect in preparation of the medicine for treating and / or preventing the endometrial hyperplasia disease, preparation ofthe medicine for treating and / or preventing Cd-induced abnormal proliferation of the endometrial cells and preparation of the medicine for protecting the endometrium.

The invention relates to a method for separating, culturing and purifying endometrial cells, and belongs to the technical field of human cell separation and culture. The method comprises the followingsteps: taking an exfoliated endometrial tissue, adding a cleaning solution for cleaning, and cutting the endometrial tissue into pieces for later use after cleaning is completed; adding digestive juice I containing type I collagenase into the cut endometrial tissue, stopping digestion by using an endometrial cell culture solution after digestion is finished, carrying out filtering by using a 400-mesh filter screen, separating filtrate from filter residues, centrifuging the filtrate, and re-suspending the precipitate by using an endometrial cell culture solution to obtain a suspension; and adding trypsin-containing digestive juice II into the filter residues for digestion, stopping digestion by using an endometrial cell culture solution, carrying out centrifuging, taking precipitate, re-suspending the precipitate by using an endometrial cell culture solution to obtain a suspension, and finally, carrying out mixed culture on the suspension and the suspension obtained in the previous step. According to the method for separating, culturing and purifying endometrial cells, more endometrial cells can be obtained more efficiently aiming at the defects of extraction by a conventional method.

The invention relates to a fat stem cell membrane sheet, particularly relates to a method for constructing a fat stem cell membrane sheet and application, and belongs to the fields of tissue engineering and regenerative medicine. The method comprises the following steps: step 1) selecting and using fat stem cells as seed cells and constructing a cell membrane sheet; step 2) enabling endometrial cells to be applied to induced cocultured cells; and step 3) constructing the fat stem cell membrane sheet by adopting chemical induction and coculture modes. Exogenous factor (TGF-beta 1, EGF, PDGF-BBand 17-beta estradiol) are added and are compounded with the endometrial cells to perform coculture by utilizing a membrane sheet culture medium, human fat stem cells are induced to form membranes; the expression quantity of each epithelial marked mRNA of the membrane sheet which is induced by the membrane sheet culture system is up-regulated; and immunofluorescence shows that protein CK18 expresses positive and prompts that part of stem cells are transformed into epithelial cells. The membrane sheet induced by the system is more effective in the aspect of repairing endometrial epithelium.

The invention discloses an endometrial pathological image classification method. The method comprises the following steps: 1, collecting an endometrial image and a clinical feature corresponding to the endometrial image; 2, constructing an endometrial group image database; 3, constructing and training an image classification network; 4, testing an image classification network; and 5, classifying endometrial pathological images. According to the method, whether endometrial cells, endometrial cell communities and endometrial micro-tissues are diseased or not needs to be rapidly identified, rapid segmentation of historical endometrial images is achieved by improving a dilution scanner network skipping path, the film reading speed is increased, two LSTM models and an Incept ion-v3CNN image model are used for synchronously processing image features, clinical features are obtained. The endometrial cell and micro-tissue pathological classification is vividly, intuitively, quickly and accurately carried out, so that a foundation is laid for the development of endometrial cancer screening work, and the problems of large workload, low working efficiency and the like of crowd screening are solved.

The invention provides a fluorescent quantization PCR kit for detecting cow endometrial cell inflammatory reaction. The fluorescent quantization PCR kit comprises a PCR buffer solution, a TaqDNA polymerase mixture, an SYBRGreen dye, and two pairs of primers, wherein the two pairs of primers include a primer 1 and a primer 2, the primer 1 comprises a forward primer 5'-TATGACCAGGGACCAGGTAC-3' and a reverse primer 5'-CTCTGCCCTTAGGGACTCACAG-3', and the primer 2 comprises a forward primer 5'-ATCGGCAATGAGCGGTTCC-3' and a reverse primer 5'-GTGTTGGCGTAGAGGTCCTTG-3'. The kit disclosed by the invention can be simple and fast, and can relatively and quantitatively detect the transcriptional level of an amyloid protein (SAA) gene in a cow endometrial cell, so as to provide reference for quickly judging whether the dairy cow has recessive endometritis.

The invention discloses a method for culturing endometrial cells, which comprises the following steps: adding a stem cell exosome freeze-dried powder aqueous solution and human serum albumin into a DMEM / F12 culture medium to obtain a cell culture system; resuspending endometrial cells obtained by separating endometrial tissues by using a DMEM / F12 culture medium, adding the resuspended endometrial cells into the cell culture system, then carrying out centrifugal separation, taking precipitates, resuspending the precipitates by using the cell culture system, and inoculating a culture bottle for culturing to complete the culture of the endometrial cells. According to the method, when the cells grow to the seventh day, the cell density reaches 90%, cell passage can be carried out, and the cell activity is good.

The present invention provides biomarkers for the diagnosis and prognosis of endometriosis. Generally, the methods of this invention find use in diagnosing or for providing a prognosis for endometriosis by detecting the expression levels of biomarkers, which are differentially expressed (up- or down-regulated) in endometrial cells from a patient with endometriosis. Similarly, these markers can be used to diagnose reduced fertility in a patient with endometriosis or to provide a prognosis for a fertility trial in a patient suffering from endometriosis. The present invention also provides methods of identifying a compound for treating or preventing endometriosis. Finally, the present invention provides kits for the diagnosis or prognosis of endometriosis.

The invention discloses an endometrial cancer cell detection method, system and equipment and a storage medium. The method comprises the following steps: preparing an endometrial cell pathological section; preliminarily screening images containing positive cells by using CNN; performing intelligent detection on cancer cells by using the improved Faster R-CNN target detection model; and performing statistics on each index, sending the WSI generated blocks into network prediction, and then merging identification results. In the target acquisition part, a data set is normalized and expanded by using a self-adaptive random clipping method on the aspect of data; in the model, an improved CNN model and a two-stage Faster RCNN model are taken as basic models, diagnosis experience of doctors is introduced, and diagnosis indexes are extracted to jointly act to train a more accurate model. The accuracy rate of the endometrial cancer cell detection method is higher than that of a classification method, meanwhile, the confidence coefficient and the position information of the WSI can be given, and in addition, the invention further provides a preparation method of a more effective endometrial cell pathological section image data set. The method is applied to the field of medical image target detection.

The present invention belongs to the tissue engineering field and is especially a preparation process of artificial endometrium. New type biodegradable material is used in making stereo rack, endometrium cell of experiment animal is used as seed cell, and artificial endometrium is in vitro constituted via special culture process. The artificial endometrium is a 3D structure, has the similar shapeand function to the in vivo endometrium, can secrete extracellular matrix component and can emulate the configuration and function of in vivo endometrium. The present invention provides the research of embryo nidatin mechanism with ideal platform and provides treating way for endometrium damage caused by induced abortion, tuberculosis, etc.

Provided are a cervical brush and a manufacturing process thereof. The cervical brush comprises a brush handle, a fixing piece and a brush head, the brush head is provided with a connecting rod and bristles, the connecting rod is provided with a blocking position and an end, and the end is provided with a round end portion and a collecting portion. The manufacturing process of the cervical brush comprises the following steps: screening the brush head, manufacturing the end, drying and shaping the end, processing the connecting rod, heating the connecting rod and mounting the brush handle, so that the brush head produced by a method for winding an iron wire is effectively and stably fixed on the brush handle; after successful installation, through quality detection, overall sterilization and cervical brush packaging, the quality and sanitation degree of the cervical brush are guaranteed. According to the manufacturing process of the cervix brush, the brush head and the brush handle can be firmly and fixedly connected, the stability degree of insertion fit connection is improved, the round end portion on the brush head effectively provides a judgment basis of the insertion depth for a user, and the collecting portion can effectively collect cells on the inner wall of the bottom of the uterus, and help is provided for a patient to deeply obtain the endometrial cells.

The present invention relates to newly identified nucleic acid molecules and polypeptides present in normal and neoplastic endometrial cells, including fragments, variants and derivatives of the nucleic acids and polypeptides. The present invention also relates to antibodies to the polypeptides of the invention, as well as agonists and antagonists of the polypeptides of the invention. The invention also relates to compositions containing the nucleic acid molecules, polypeptides, antibodies, agonists and antagonists of the invention and methods for the use of these compositions. These uses include identifying, diagnosing, monitoring, staging, imaging and treating endometrial cancer and non-cancerous disease states in endometria, identifying endometrial tissue, monitoring and identifying and / or designing agonists and antagonists of polypeptides of the invention. The uses also include gene therapy, production of transgenic animals and cells, and production of engineered endometrial tissue for treatment and research.

The invention discloses a method for measuring intracellular chlorine concentration or chloride channel gene CFTR expression of human endometrial stromal cells in a menstrual cycle so as to predict prognosis of endometrial deciduation related diseases of the human endometrial stromal cells. Intracellular chlorine concentration up-regulation and chlorine channel gene CFTR expression down-regulation in endometrial stromal cells in a menstrual cycle can hinder deciduation of the endometrial cells, and finally, repeated abortion / repeated implantation failure is caused. Restoration of CFTR expression and / or reduction of chloride ion concentration in endometrial stromal cells in a menstrual cycle can improve uterine decidualization and improve the outcome of recurrent abortion / recurrent implantation failure. The invention develops a new field for clinical prediction and treatment of recurrent abortion / recurrent implantation failure, and has a good application prospect.

The invention discloses an endometrial cell sampler which comprises an outer sleeve and an inner rod arranged in the outer sleeve and capable of extending, contracting and moving in the outer sleeve.Each of the head end of the inner rod and that of the outer sleeve is provided with a sampling portion. The endometrial cell sampler has little irritation to an examined muscle body portion and has good sampling effect, and samples on the sampling portions are protected effectively.

A method for growing polarized endometrial cells, said method comprising: (a) disposing endometrial cells on a scaffold, said scaffold comprising a silica-based glass composition, characterized by multi-modal porosity, said scaffold being to define a top side and a bottom side; (b) providing nutrients to said top and bottom sides of said scaffold and an environment to grow polarized endometrial cells on said scaffold.

The invention relates to an adipose stem cell membrane, in particular to a method and application for constructing an adipose stem cell membrane. It belongs to the field of tissue engineering and regenerative medicine. The method comprises the following steps: step 1) selecting adipose stem cells as seed cells to construct cell membranes; step 2) using endometrial cells for induced co-culture cells; step 3) constructing adipose-derived stem cell membranes by means of chemical induction and co-cultivation piece. The present invention utilizes cell patch medium to add exogenous factors (TGF-β1, EGF, PDGF-BB, 17-β estradiol) to co-culture endometrial cells to induce human adipose stem cells to form a film. This patch culture system , the mRNA expression of each epithelial marker in the induced membrane was up-regulated, and immunofluorescence showed that the protein CK18 was positive, suggesting that some stem cells transformed into epithelial cells. The membrane sheets induced by this system are more effective in repairing the endometrial epithelium.

Methods for the detection and diagnosis of endometriosis are disclosed herein. More specifically, sensitive single-cell expression analysis methods to detect specific gene expression patterns in cellpopulations obtained from endometrial cells are provided.

The invention discloses a method for identifying peripheral blood circulation endometrial cells and application. The identification method according to the present invention comprises a step of selecting, as candidate cells, PECAM1-, DAPI +, and PTPRC-from an enriched liquid phase obtained by removing erythrocytes and leukocytes from peripheral blood from a subject, cells in which endometrial markers are positive. According to the method, the identification accuracy of the circulating endometrial cells can be greatly improved.