31 results about "Recurrent Abortions" patented technology

The invention relates to a microRNA marker for diagnosing the natural recurrent abortion, and in particular relates to a microRNA marker hsa-miR-3074 used for distinguishing the tissue suffering from natural recurrent abortion and the normal tissue. The inspection proves that the specific microRNA marker can be used for effectively diagnosing and / or predicating the natural recurrent abortion. The invention further relates to applications of the marker microRNA hsa-miR-3074 and a kit used for detecting the natural recurrent abortion.

The invention discloses a gene mutant and application thereof. Compared with SEQ ID No. 1, the gene mutant has at least one mutation selected from a group consisting of c.1501C>T mutation and c.17C>T mutation; or compared with SEQ ID No. 2, the gene mutant has at least one mutation selected from a group consisting of c.270G>T mutation and c.1191C>G mutation. Whether a biological sample is prone to recurrent abortion can be effectively detected by detecting existence of the gene mutant in the biological sample.

The invention relates to a method for constructing an animal model, in particular to an animal experimental study model for pathogenesis study, diagnosis and treatment of autoimmune recurrent abortion and anti-phospholipid antibody syndrome. The autoimmune recurrent abortion mouse model is established by injecting human beta2GP-1 into a BALB / c mouse uterine cavity. The method for constructing the animal model is safe, reliable, simple and economic, reduces the modeling time of the autoimmune recurrent abortion (RSA) mouse model, improves the modeling success rate and modeling efficiency, and has good application prospect.

The invention provides a primer combination for detecting microdeletion or / and microduplication of recurrent abortion, an MLPA probe, a gene chip and a kit, and relates to the field of biotechnology.The primer combination has a nucleotide sequence as shown in SEQ ID NO.1-21, can be made into MLPA probes, gene chips, kits or constructs, and recombinant cells, and can also be used as a main component of a system for rapidly screening biological samples susceptible to recurrent abortion. The primer combination provided by the invention can help simultaneously detect recurrent abortion microdeletion or / and microduplication at multiple sites, has good specificity, sensitive response, short detection time and simple method, can detect from point mutation to large chromosome deletion / insertion,has low cost and high clinical application value.

The invention relates to the technical field of maternal and infant health monitoring, in particular to a recurrent abortion maternal and infant health follow-up visit tracking analysis system. The system comprises an information acquisition module used for inputting medical record information of a pregnant woman, a data tracking module used for tracking the position of the pregnant woman, a specific activity area and change data of physiological indexes; an analysis and management module which is used for reading medical record information of the information acquisition module and the data tracking module, performing information screening, preliminarily screening out recurrent spontaneous abortion high-risk groups, performing recurrent spontaneous abortion peripregnancy cause deduction bycombining physiological index data of pregnant women tracked in the peripregnancy period, and finally giving out diagnosis suggestions; and a follow-up visit management module which determines a follow-up visit report and follow-up visit time according to the index value and the health assessment result. According to the invention, tracking analysis can be carried out on pathological factors of recurrent spontaneous abortion, diagnosis suggestions are finally given according to pathogenesis deduction, and tracking and research of perinatal pregnancy-related diseases can be carried out by a plurality of cross-institution and cross-region cooperation units.

The invention discloses a natural pharmaceutical composition for treating recurrent abortion, and the natural pharmaceutical composition is characterized by comprising the following effective component raw materials of seventeen drugs of radix rehmanniae, artemisia annua, salvia chinensis, dogwood, parasitic loranthus, acanthopanax fruit, asarum pulchellum, cratoneuron filicinum, bamboo fungus, fruit of argy wormwood, ventilago leiocarpa benth, white atractylodes rhizome, ginseng, pig blood, holothuria leucospilota, semen canavaliae and licorice. The natural pharmaceutical composition for treating recurrent abortion, according to traditional Chinese medicine and pharmacy theory, aims at the etiology and pathogenesis of recurrent abortion, promotes simultaneously the effects of tonifying liver and kidney, invigorating spleen and supplementing qi, clearing heat and nourishing Yin, generating blood and benefitting blood, promoting circulation and removing stasis, and has good clinical curative effect on the recurrent abortion.

The invention discloses a method for predicting immune dysfunction of a recurrent abortion population during pregnancy, which comprises the following steps: separating platelets in plasma of URSA patients by using a differential centrifugation method, then carrying out nuclear genome DNA interference treatment digestion on platelet precipitation by using DNA restriction enzyme, then directly carrying out bisulfite treatment on a digestion product, and finally predicting immune dysfunction of the recurrent abortion population during pregnancy. A pyrosequencing method is combined to detect the methylation level of respiratory chain related gene loci, including MT-ATP8, MT-COX1, MT-COX2, MT-COX3, MT-ND5 and MT-TRNA, in the platelet mtDNA; the method has the advantages that the methylation level is detected by selecting the mitochondrial sites related to URSA, and the diagnosis efficiency of the biomarker is evaluated by using AUC, so that the prediction capability on the onset risk of the blood coagulation dysfunction is improved.

The invention belongs to the technical field of traditional Chinese medicine and relates to a pharmaceutical preparation for treating yin-asthenia-generating-intrinsic-heat type recurrent abortion caused by blocking antibody deficiency and a preparation method thereof.The pharmaceutical preparation is meticulously prepared from 11 medicinal materials including radix rehmanniae praeparata, all-grass of intermediate peashrub, glossy privet fruits, Chinese holly leaves, radix paeoniae alba, herbal cistanche, all-grass of eleusinoides galingale, lalang grass rhizomes, common yam rhizomes, rhizoma cyperi and roots of stellaria yunnanensis.All the medicinal materials are under compatibility to achieve the effects of tonifying the kidney, soothing the liver, nourishing yin to clear heat, cooling blood to remove blood stasis, releasing liver depression, promoting qi circulation, promoting channel tropism of blood circulation, removing heat, calming blood, removing stasis and harmonizing collaterals; immunity is regulated, increase of blocking antibodies is promoted, yin-asthenia-generating-intrinsic-heat symptoms are relieved, and therefore the treatment effect is achieved.

The invention relates to a circular RNA marker and application thereof in diagnosis and prediction of recurrent abortion pathological pregnancy. Specifically, the invention provides application of the circular RNA marker circular RNA hsacirc0007291 and a detection reagent thereof in diagnosis and prediction of the recurrent abortion (RSA), including preparation of a diagnostic reagent or a kit for diagnosing / predicting the RSA. Researches show that the circular RNA marker disclosed by the invention can be used as a marker for diagnosing RSA and predicting RSA generation, and has high sensitivity and specificity.

The invention belongs to the field of reproductive medicine. According to the technical scheme provided by the invention, the follicular fluid HRG and C4B protein expression level is used as an early warning method for unexplained recurrent abortion. The method is not used for disease diagnosis and treatment, and comprises the following steps of 1) collection and preservation of follicular fluid: picking up an ovum crown compound from the collected follicular fluid at a constant temperature, then collecting the remaining follicular fluid, centrifuging at 1500 rpm to take a supernatant, subpackaging, and cryopreserving at -80 DEG C, (2) carrying out ELISA determination on the protein concentration of C4B and HRG follicular fluid: carrying out corresponding protein concentration detection on the follicular fluid by adopting an ELISA detection kit of corresponding protein, and 3) result statistics and analysis. The method has a certain prediction value for the pregnancy outcome of the URSA patient.

The invention discloses a method for predicting abnormal blood coagulation function of recurrent abortion crowds during pregnancy, which comprises the following steps: separating platelets in plasma of URSA patients by using a differential centrifugation method, then carrying out nuclear genome DNA interference treatment digestion on platelet precipitation by using DNA restriction enzyme, then directly carrying out bisulfite treatment on digestion products, and finally predicting the abnormal blood coagulation function of the recurrent abortion crowds during pregnancy. A pyrosequencing method is combined to detect the methylation level of respiratory chain related gene loci, including MT-ATP8, MT-COX1, MT-COX2, MT-COX3, MT-ND5 and MT-TRNA, in the platelet mtDNA; the method has the advantages that the methylation level is detected by selecting the mitochondrial sites related to URSA, and the diagnosis efficiency of the biomarker is evaluated by using AUC, so that the prediction capability on the onset risk of the blood coagulation dysfunction is improved.

The invention discloses a method for measuring intracellular chlorine concentration or chloride channel gene CFTR expression of human endometrial stromal cells in a menstrual cycle so as to predict prognosis of endometrial deciduation related diseases of the human endometrial stromal cells. Intracellular chlorine concentration up-regulation and chlorine channel gene CFTR expression down-regulation in endometrial stromal cells in a menstrual cycle can hinder deciduation of the endometrial cells, and finally, repeated abortion / repeated implantation failure is caused. Restoration of CFTR expression and / or reduction of chloride ion concentration in endometrial stromal cells in a menstrual cycle can improve uterine decidualization and improve the outcome of recurrent abortion / recurrent implantation failure. The invention develops a new field for clinical prediction and treatment of recurrent abortion / recurrent implantation failure, and has a good application prospect.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a medicine composition for treating recurrent abortion and a method for preparing the medicine composition. The medicine composition comprises fructus lycii, semen cuscutae, fructus broussonetiae, dried radix rehmanniae, cortex moutan, viola japonica, fruits of herba melastomatis dodecandri, cortex eucommiae, semen plantaginis, fructus alpiniae oxyphyllae and rhizoma atractylodis macrocephalae according to certain weight proportions. The medicine composition and the method have the advantages that various medicines are compatible with one another, effects of tonifying kidneys, clearing livers, nourishing and activating blood, consolidating thoroughfare and conception channels and preventing miscarriage can be realized by the aid of meaning of ' same source of livers and kidneys and joint treatment on the livers and the kidneys', ACA (anticardiolipin antibodies) can become negative by means of immunological regulation, fetuses can continue developing, the blood viscosity can be obviously reduced, the circulation flow rates can be increased, platelet aggregation and thrombosis can be resisted, placental micro-circulation can be improved, traditional Chinese medicine symptoms of patients can be treated, and the shortcoming of adverse factors of simple western medicine therapy can be overcome.

The invention discloses a biomarker for auxiliary diagnosis of recurrent abortion, and application thereof, and belongs to the technical field of biology. According to the invention, the fact that alpha1, 3 catalyzed and synthesized by fucosyltransferase 4 (FUT4) is connected with fucosylation is found for the first time, the migration, invasion and multiplication capacity of trophoblast cells is promoted, and the alpha1, 3 can be used as a biological marker for auxiliary diagnosis of recurrent abortion. It is proved that the influence of FUT4 and alpha 1, 3 connected fucosylation catalytically synthesized by FUT4 on migration invasion and multiplication capacity of trophoblast cells, which is beneficial to prediction and diagnosis of recurrent abortion and deep understanding of an embryo implantation mechanism, and also can provide a new theoretical basis for clinical treatment of recurrent abortion taking sugar as a target spot.

The invention relates to application of aspirin in preparation of a medicine for preventing and treating unexplained recurrent abortion. According to the invention, a small dose of aspirin is added into the drinking water of the unexplained recurrent abortion mouse, so that the embryo absorption rate is reduced, the inflammatory response of the decidua tissue of the mouse is improved, the HMGB1 level of the serum and the decidua tissue of the mouse is reduced, and the proinflammatory response further caused by the high-level HMGB1 of the unexplained recurrent abortion mouse is prevented; further, the pregnancy rate of the mice with unexplained recurrent abortion is effectively protected and treated.

The invention discloses application of a vaginal microorganism in recurrent abortion. The microorganism is Rhodoluna. The microorganism marker has high sensitivity in predicting recurrent abortion, and can be used for warning a subject by detecting the abundance of the microorganism marker, and further reducing risks by adjusting diet or medical intervention.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a medicine composition for treating recurrent abortion due to positive anticardiolipin antibodies and a method for preparing the medicine composition. The medicine composition comprises semen cuscutae, herba epimedii, radix rehmanniae preparata, caragana rosea, herba cistanches, roots of polyalthia nemoralis, cortex eucommiae, cyperus michelianus, herba leonuri and clematis chrysocoma. The medicine composition and the method have the advantages that all components of the medicine composition are compatible with one another, accordingly, effects of nourishing kidneys, enriching qi and promoting blood circulation to remove blood stasis can be realized, congenital and postnatal attributes can be jointly nourished, yin and yang in the kidneys can be regulated and invigorated again to be in balance, and definite curative effects of reducing or eliminating the serum anticardiolipin antibodies can be realized; symptoms of kidney deficiency and blood stasis of patients who suffer from excessive maternal-fetal immune recognition type recurrent spontaneous abortion can be treated, the obvious curative effects can be realized, and the medicine composition is free of side effects.

The invention relates to an abortion marker and application thereof in diagnosis and prediction of recurrent abortion pathology pregnancy. Specifically, the invention provides applications of the RSA marker circular RNA hsacirc0008362 and a detection reagent thereof in diagnosis and prediction of the recurrent abortion (RSA), and the RSA marker circular RNA hsacirc0008362 is used for preparing a diagnostic reagent or a kit for diagnosing / predicting the RSA. Researches show that the RSA marker disclosed by the invention can be used as a marker for diagnosing RSA and predicting RSA occurrence, and has high sensitivity and specificity.

The invention discloses a biomarker for auxiliary diagnosis of recurrent abortion and application thereof, and belongs to the technical field of biology. It is found that the migration, invasion and proliferation capacities of trophoblast cells can be changed by changing the LeY oligosaccharide on the MEST, and the MEST and the LeY oligosaccharide on the MEST can serve as biological markers for auxiliary diagnosis of the recurrent abortion, so that prediction and diagnosis of the recurrent abortion are facilitated, the embryo implantation mechanism is deeply understood, and the development of the recurrent abortion is promoted. And a new theoretical basis can be provided for clinical treatment of recurrent abortion taking glycoprotein as a target spot.

The invention relates to a traditional Chinese medicine composition for treating recurrent abortion and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 9-20 parts of codonopsis pilosula, 9-20 parts of bighead atractylodes rhizome, 10-20 parts of dried rehmannia root, 10-20 parts of white paeony root, 10-20 parts of scutellaria baicalensis, 9-20 parts of perilla stem, 10-20 parts of eucommia ulmoides, 10-20 parts of teasel root, 10-20 parts of semen cuscutae, 10-20 parts of parasitic loranthus, 10-30 parts of astragalus membranaceus, 7-10 parts of ligusticum wallichii, 7-10 parts of angelica sinensis, 20-30 parts of ramie root and 20-30 parts of pumpkin base. The whole formula has the effects of invigorating spleen and tonifying kidney to strengthen abortion, nourishing blood and harmonizing blood to treat deficiency and excess inclusion, clearing heat and nourishing yin, and achieving a good effect of miscarriage prevention. Animal experiments and clinical experiments show that the traditional Chinese medicine can effectively prevent and treat recurrent abortion.

The invention belongs to the technical field of traditional Chinese medicines and relates to pharmaceutical composition for preventing and treating qi and blood deficiency type recurrent abortion and a preparation method of the pharmaceutical composition. The pharmaceutical composition is prepared from raw medicinal materials as follows: roots or stems of evergreen Mucuna, manyprickle acathopanax roots, small-leaved embelia roots or stems, danshen roots, alpine swertia, conic gymnadenia tubers, Poria cocos, largehead atractylodes rhizomes, dodder seeds and nutgrass galingale rhizomes. All medicines are combined, have effects of reinforcing qi, nourishing blood, tonifying the spleen and reinforcing the kidney and promote the nature by cultivating and supplementing the nurture, so that the nature and the nurture reinforce each other; the composition is exact in clinical effect, lower in side effect, better in patient compliance, convenient to take, capable of effectively reducing the burden and relieving pain of patients and treating both symptoms and root causes and worthy of clinical wide popularization and application.

The invention belongs to the technical field of traditional Chinese medicines, and relates to a medicinal preparation for treating recurrent abortion and a method for preparing the medicinal preparation. The medicinal preparation comprises dried radix rehmanniae, folium ilicis cornutae, rhizoma cibotii, herba taxilli, limonium sinense, fructus amomi, verbena officinalis, euonymus fortunei and radix salviae miltiorrhizae according to certain category proportions. The medicinal preparation and the method have the advantages that various medicines are compatible with one another, accordingly, effects of enriching yin, nourishing kidneys, reinforcing qi, nourishing blood, clearing livers to relieve heat and promoting blood circulation to remove blood stasis can be realized, patients have high kidney energy, consolidated thoroughfare and conception channels, stasis and heat can be eliminated, antibodies of the patients can be suppressed or eliminated, anticardiolipin antibodies can become negative, and the patients can get ready for conception.

The invention relates to a traditional Chinese medicine composition for treating recurrent miscarriage combined with antiphospholipid syndrome. The traditional Chinese medicine composition is made of the following raw materials in parts by weight: Angelica 9-11 parts, Cuscuta 14-16 parts, Dipsacus 14-16 parts 16 parts, 14-16 parts of Sangjisheng, 11-13 parts of Chuanxiong, 5-7 parts of donkey-hide gelatin, 2-4 parts of licorice. The invention also provides the application of the traditional Chinese medicine composition. The invention combined with the standardized western medicine treatment plan can significantly improve the live birth rate of patients with recurrent miscarriage combined with antiphospholipid syndrome, and has stronger power of nourishing and activating blood.

The invention relates to the technical field of traditional Chinese medicine compounds, in particular to a traditional Chinese medicine composition for preventing and treating recurrent abortion. The traditional Chinese medicine composition for preventing and treating recurrent abortion is prepared from 25-35 parts of semen cuscutae, 10-20 parts of herba taxilli, 10-20 parts of radix dipsaci, 8-14 parts of fried pollen typhae, 8-13 parts of ginseng, 7-12 parts of pericarpium citri reticulatae and 20-35 parts of caulis spatholobi, the safety of the traditional Chinese medicine composition is high, the miscarriage prevention success rate can be increased conveniently, and the clinical curative effect can be improved by combining the traditional Chinese medicine composition with other medicines.

The invention provides a traditional Chinese medicine composition for preventing recurrent abortion and application, and relates to the technical field of traditional Chinese medicine. The traditional Chinese medicine composition for preventing recurrent miscarriage comprises dodder, parasitic loranthus, dipsacus asperoid, Codonopsis pilosula, Chinese yam, radix paeoniae alba, donkey-hide glue, roasted rhizoma atractylodis macrocephalae, prepared radix liquiritiae, cornus officinalis and radix ophiopogonis. The traditional Chinese medicine composition can simplify the diagnosis and treatment difficulty of recurrent spontaneous abortion and solve the problem of differentiation of syndrome unavailable, meanwhile, the traditional Chinese medicine composition is safe and reliable and has no toxic side effect, and the curative effect is obvious; prognosis of the second pregnancy can be improved significantly, the miscarriage preventing success rate of recurrent abortion is increased; and the traditional Chinese medicine composition for preventing recurrent abortion and application are easy to learn and worth to be applied and popularize.

The invention belongs to the technical field of traditional Chinese medicine and relates to a medicine composition used for a polycystic ovarian syndrome and recurrent abortion and a preparation method thereof. The medicine composition is prepared from Chinese holly leaves, roses, glossy privet fruits, Stellaria yunnanensis, broadleaf sedge roots, melastoma dodecandrum fruits, Chinese taxillus twigs, shavings of omei mountain bamboo and ramie roots. The selected herbs are appropriate in compatibility, are formulated refinedly and supplement one another in function to achieve a remarkable treatment effect; the herbs restrict one another and assist one another in medicine property, and toxic and side effects are small; the medicine composition meets theories of traditional Chinese medicine and pharmacy and modern medicine and pharmacy to achieve the functions of soothing the liver and resolving depression, nourishing yin and clearing heat, regulating qi and blood and nourishing the blood and preventing abortion harmoniously, and is particularly researched for the syndrome of stagnant fire in the gan channel. Clinical observations show that after a treatment group uses the medicine for treatment, the curing rate and effective rate are obviously higher than these of a comparison group.

The invention relates to an application of PRMT3 in diagnosis or treatment of spontaneous abortion. By analyzing and comparing the expression level of the PRMT3 in the decidua of normal pregnancy and recurrent abortion (RSA) patients, the phenomenon that the expression level of the PRMT3 is increased in decidua tissues and decidua macrophages of the RSA patients is found; the apoptosis of the trophoblast HTR-8 / SVneo can be reduced by inhibiting the PRMT3 in vitro; an in-vivo animal test shows that the PRMT3 inhibitor can reduce the embryo absorption rate of a CBA / J*DBA / 2 spontaneous abortion mouse model, so that a new method is provided for clinical diagnosis and treatment of spontaneous abortion.

The invention discloses a gene mutant and application thereof. Compared with SEQ ID No. 1, the gene mutant has at least one mutation selected from a group consisting of c.1501C>T mutation and c.17C>T mutation; or compared with SEQ ID No. 2, the gene mutant has at least one mutation selected from a group consisting of c.270G>T mutation and c.1191C>G mutation. Whether a biological sample is prone to recurrent abortion can be effectively detected by detecting existence of the gene mutant in the biological sample.