Product
Patsnap Eureka
For R&D, Patsnap Eureka makes reading and utilizing patents & technical documents easy.
Patsnap Eureka AIR
Designed for self-driven R&D workflows. Generate viable solutions, solve complex R&D challenges, empower your innovation with AI.
Patsnap Eureka Materials
Designed for material experts only. Revolutionize your material R&D, from search, analyze, to developing new materials.
Feature
TechResearch
Generate reliable direction feasibility study reports for your R&D in just a few steps.
TechSeek
Discover and master advanced knowledge NOW. Basics, ideas, possibilities, all at once.
TechMind
As an expert in R&D Theories, TechMind can generates customized viable solutions instantly.
TechRisk
Analyze your overall solution with one click, know your potential R&D risks in advance.
TechMonitor
Get weekly tech updates, stay abreast of the latest tech innovations and key insights.
Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
32 results about "Decidua" patented technology
Efficacy Topic
Property
Owner
Technical Advancement
Application Domain
Technology Topic
Technology Field Word
Patent Country/Region
Patent Type
Patent Status
Application Year
Inventor
The decidua is the modified mucosal lining of the uterus known as the endometrium that forms in preparation for pregnancy. It is formed in a process called decidualization under the influence of progesterone. Endometrial cells become highly characteristic. The decidua forms the maternal part of the placenta and remains for the duration of the pregnancy. It is shed off during childbirth — hence why the term is used, "decidua" having the meaning of falling away, as in the word deciduous.
Multipotent stem cells derived from placenta tissue and cellular therapeutic agents comprising the same
InactiveUS20070243172A1Negative immunological responseBiocideArtificial cell constructsGerm layerDisease
The present invention relates to placenta tissue-derived multipotent stem cells and cell therapeutic agents containing the same. More specifically, to a method for producing placenta stem cells having the following characteristics, the method comprising culturing amnion, chorion, decidua or placenta tissue in a medium containing collagenase and bFGF and collecting the cultured cells: (a) showing a positive immunological response to CD29, CD44, CD73, CD90 and CD105, and showing a negative immunological response to CD31, CD34, CD45 and HLA-DR; (b) showing a positive immunological response to Oct4 and SSEA4; (c) growing attached to plastic, showing a round-shaped or spindle-shaped morphology, and forming spheres in an SFM medium so as to be able to be maintained in an undifferentiated state for a long period of time; and (d) having the ability to differentiate into mesoderm-, endoderm- and ectoderm-derived cells. Also the present invention relates to placenta stem cells obtained using the production method. The inventive multipotent stem cells have the ability to differentiate into muscle cells, vascular endothelial cells, osteogenic cells, nerve cells, satellite cells, fat cells, cartilage-forming cells, osteogenic cells, or insuline-secreting pancreatic β-cells, and thus are effective for the treatment of muscular diseases, osteoporosis, osteoarthritis, nervous diseases, diabetes and the like, and are useful for the formation of breast tissue.
Owner:RNL BIO
Placenta stem cell bank construction method and placenta tissue resuscitation method
InactiveCN104480533ALong storage timeKeep aliveEmbryonic cellsGerm cellsDrug biological activityResuscitation
The invention discloses a placenta stem cell bank construction method and a placenta tissue resuscitation method, and relates to placenta stem cell bank construction methods. The placenta stem cell bank construction method comprises the following steps: after non-bacterial cleaning treatment to placenta tissues of at least three parts, the steps of tissue peeling, further sterilizing, shearing to be thin, protecting using a refrigerant, programmed freezing, cryogenic temperature temporary storage, long term storage with nitrogen canisters, and the like are carried out, and the placenta tissue with biological activities can be preserved for a long term. The placenta tissue preserved through the method can be used for the construction of a stem cell bank, resuscitation, enzymic digestion, cultivation, expansion, quality inspection and the like are performed after selective tissue part resuscitation according to required cell types, so as to obtain placenta amniotic membrance epithelial cells, placenta amniotic membrance mesenchyme, placenta chorion mesenchymal stem cell, placenta decidua serotina mesenchymal stem cells and the like. The method provided by the invention has the advantages that operation steps are simplified, manual intervention errors are reduced, the efficiency is improved, more stem cell resources are preserved, and a basis is provided for further personalized stem cell customization.
Owner:天晴干细胞股份有限公司
Method for preparing decidua mesenchymal stem cell
ActiveCN104560870APollution prevention methodsReduced Chances of ContaminationSkeletal/connective tissue cellsSerum free mediaDigestion
The invention provides a method for preparing a decidua mesenchymal stem cell. The method comprises the following steps: performing placenta asepsis by taking the placenta of a male full-term fetus as a raw material; separating a decidual tissue; identifying the decidual tissue and identifying whether the decidua mesenchymal stem cell comes from a maternal tissue; culturing the decidua mesenchymal stem cell; freeze-storing the decidua mesenchymal stem cell; and reviving the decidua mesenchymal stem cell. The method provided by the invention has the technical characteristics that the sampled fetus is male fetus placenta; after the decidual tissue is obtained, sex determination is performed to identify whether the decidual tissue comes from a parent body and is free of pollution of a daughter tissue; by adopting a bacterial pollution prevention method, the pollution possibility is reduced from a sampling source; the surface is washed for a plurality of times, so that the pollution possibility is effectively reduced; a single enzyme is used for digestion to simplify the procedures; a serum-free medium is used for culture to reduce the use of an animal source component; the cell is stable in performance; a long-term in-vitro culture process of the decidua mesenchymal stem cell can be maintained; and the cellular morphology, multiplication capacity, MSC surface marking expression capacity, differentiative capacity and the like of the cell can be maintained.
Owner:江苏省北科生物科技有限公司 +1
Method of culturing pluripotent stem cells using extracellular matrix from fetal membrane-derived cells
InactiveUS20100167398A1Sufficient quantitative supplyPerformed safely and efficientlyBioreactor/fermenter combinationsBiological substance pretreatmentsCell-Extracellular MatrixInduced pluripotent stem cell
Provided are a method of culturing pluripotent stem cells in the presence of a decidua-derived cell or an extracellular matrix derived from the cell, that enables safe and efficient maintenance culture and derivation of pluripotent stem cells; a culture agent for pluripotent stem cells, that comprises a decidua-derived cell or an extracellular matrix derived from the cell; and other means for developing or performing the method.
Owner:RIKEN
Preparation and cryopreservation method and application of human placental chorionic tissues
ActiveCN108812643AGuaranteed survivalHigh activityCulture processDead animal preservationVascular tissueFresh Tissue
The invention discloses a preparation and cryopreservation method of human placental chorionic tissues. The method comprises the following steps: (1) separation and cryopreservation of a placental amnion and a decidua; (2) separation and cryopreservation of placenta subchorionic large vascular tissues; 3) separation and cryopreservation of the placental chorionic tissues; and (4) treatment and cryopreservation of the placental chorionic tissues. The invention also provides a method for resuscitation after cryopreservation. The cryopreservation method facilitates the improvement of the activityof cryopreserved tissues and cells, the morphology, the function and the structure of resuscitated cryopreserved tissues are same to those of fresh tissues, the overall survival rate of cells in thetissues reaches 90% or above, and the preserved tissues can be used in the field of separation of stem cells and epithelial cells, and also can be used in the field of tissue engineering transplantation.
Owner:YINFENG BIOLOGICAL GRP +1
Cellular therapeutic agent for incontinence or urine comprising stem cells originated from decidua or adipose
InactiveUS20110059052A1Preventing and treating urinary incontinencePreventing and urinary incontinenceBiocideMuscular disorderLeak point pressureUrethral sphincter
The present invention relates to a cellular therapeutic agent for treating urinary incontinence, and more particularly to a cellular therapeutic agent for treating urinary incontinence, which contains stem cells derived from the decidua of the placenta or menstrual fluid or stem cells derived from adipose. The decidua-derived stem cells or adipose-derived stem cells show the effects of increasing leak point pressure and urethral sphincter contractility, and thus are useful as an agent for treating urinary incontinence.
Owner:RNL BIO
Application of fructose 1,6-diphosphate (FDP) in preparation of fetus protection medicine for spontaneous abortion
The invention belongs to the technical field of medicines, and in particular discloses application of fructose 1,6-diphosphate (FDP) in preparation of a fetus protection medicine for spontaneous abortion. In vitro and in vivo animal experiments show that the concentration of the FDP in peripheral blood and decidual tissues of patients with recurrent spontaneous abortion is decreased compared withnormal pregnancy. Compared with control pregnant mice, the model plasma and uterus FDP levels of pregnant mice with spontaneous abortion are reduced, the uterus macrophage M2 phenotype molecule expression of the pregnant mice with spontaneous abortion is decreased, the proportions of helper cells T(Th)2 and regulatory T cells are reduced, and the phenomena of poor endometrium decidualization and decreased embryonic trophoblast infiltration degree occur in an accompanied way. The results indicate that the supplement of the FDP can significantly improve the uterine immune tolerance pattern, decidualization and embryonic trophoblast infiltration in the pregnant mice with spontaneous abortion, and obviously reduces embryonic loss in the pregnant mice. Therefore, the FDP is expected to be an early-warning and diagnostic indicator for the patients with spontaneous abortion, and can be used for preparing the fetus protection medicine for the spontaneous abortion.
Owner:THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
Systematic method for preparing and cryopreserving placental tissues according to structure layer, and application thereof
ActiveCN108812642APromote healingRetain organizational structureCulture processDead animal preservationVascular tissueFresh Tissue
The invention discloses a systematic method for preparing and cryopreserving placental tissues according to a structure layer. The method comprises the following steps: (1) separation and cryopreservation of a placental amnion and a decidua; (2) separation and cryopreservation of placenta subchorionic large vascular tissues; 3) separation and cryopreservation of the placental chorionic tissues; and (4) treatment and cryopreservation of the placental chorionic tissues. The invention also provides a method for resuscitation after cryopreservation. The placental amnion, the decidua, the subchorionic large vascellum, the chorion and other tissues are respectively separated and preserved, so the improvement of the activity of cryopreserved tissues and cells is facilitated, the morphology, the function and the structure of resuscitated cryopreserved tissues are same to those of fresh tissues, the overall survival rate of cells in the tissues reaches 90% or above, and the preserved tissues can be used in the field of separation of stem cells, and also can be used in the field of tissue engineering transplantation. The systematic method for preserving various tissues of the complete placenta is provided to provide a great biological source for the studying of placenta-derived stem cells.
Owner:YINFENG BIOLOGICAL GRP
Screening method for differentially expressed proteins of heterotopic-pregnancy and non-pregnancy decidua tissue
InactiveCN106645736AImprove technical levelImprove market competitivenessIndividual particle analysisBiological testingScreening methodEctopic pregnancy
The invention discloses a screening method for differentially expressed proteins of heterotopic-pregnancy and non-pregnancy decidua tissue. The method is characterized by comprising the following steps: carrying out proteomics analysis to discover specific proteins and differential proteins of heterotopic-pregnancy and non-pregnancy decidua tissue; subjecting the specific proteins and differential proteins to separation and purification; and using the purified specific proteins and differential proteins as special biological markers for discrimination of intrauterine and extrauterine pregnancy. After pregnancy, specific proteins can be synthesized in the bodies of both a mother and a fetus. Detection of the specific proteins with different methods is beneficial for diagnosis of pregnancy. Searching for a special marker for pregnancy, especially for distinguishing intrauterine and extrauterine pregnancy, is of critical significance to determination of clinical medical liabilities and medical jurisprudence and is a great breakthrough to the basic theory of medical science.
Owner:WUHAN LABWAY MEDICAL LAB CO LTD
Method for obtaining maternal mesenchymal stem cells from placenta
PendingCN111793598AIncrease the number ofHigh purityCell dissociation methodsSkeletal/connective tissue cellsEnzyme digestionMesenchymal stem cell
The invention discloses a method for obtaining maternal mesenchymal stem cells from placenta. The method for obtaining the maternal mesenchymal stem cells from the placenta comprises the following steps: obtaining placenta tissues from a position, which are within 5cm away from the radius of an umbilical cord and are 0.5 cm away from a fetal surface, of the placenta; and carrying out enzyme digestion treatment on placenta tissues by adopting an enzyme digestion solution, and then carrying out cell culture on a product of the enzyme digestion treatment to obtain the maternal mesenchymal stem cells. According to the method disclosed by the invention, the technical problem of separating the maternal mesenchymal stem cells from the placenta is solved, the prepared maternal mesenchymal stem cells are large in quantity and high in purity, neonatal cells are not easy to mix, and the clinical application requirements can be met; the method provided by the invention solves the problems that thequantity of the maternal mesenchymal stem cells obtained from the decidua basalis is small and the decidua basalis is easy to lose, and provides a new scheme and way for obtaining the maternal mesenchymal stem cells from the placenta.
Owner:深圳华大基因细胞科技有限责任公司
Preparation and cryopreservation method and application of human placental chorionic tissues
ActiveCN108812641AGuaranteed survivalHigh activityCulture processDead animal preservationFresh TissuePlacenta^fetus
The invention discloses a preparation and cryopreservation method of human placental chorionic tissues. The method comprises the following steps: (1) cleaning a placenta, and cutting off a shed decidua along the edge of the placenta to remove the amnion; and (2) separating the amnion-removed placenta fetus chorion and large vessels to obtain remaining placenta tissues which are placental chorionictissues, cutting the placental chorionic tissues to form small blocks, cutting the small blocks to form slices, placing the slices in a cryopreservation bag or a cryopreservation tube, guiding a vitrification cryopreservation solution through a three-step process, transferring the obtained mixture into a programmable cooler, reducing the pressure to -80 to -90 DEG C, transferring the cooled mixture to liquid nitrogen, and performing cryopreservation. The cryopreservation method facilitates the improvement of the activity of cryopreserved tissues and cells, the morphology, the function and thestructure of resuscitated cryopreserved tissues are same to those of fresh tissues, the overall survival rate of cells in the tissues reaches 90% or above, and the preserved tissues can be used in the field of separation of stem cells and epithelial cells, and also can be used in the field of tissue engineering transplantation.
Owner:YINFENG BIOLOGICAL GRP
Decidua and villus pairing cDNA chip
ActiveCN107190328ARealize functional presentationImprove throughputMicrobiological testing/measurementLibrary creationClinical informationQuantitative accuracy
The invention relates to a decidua and villus pairing cDNA chip. The cDNA chip is prepared through the following method that the decidua and villus tissue of a woman with early pregnancy abortion are taken, the RNA is extracted, cDNA is formed by reverse transcription and preset in a PCR reaction plate, and cDNA chip is made. A preparation method and application of cDNA chip are further provided. The method and application have the advantages that the decidua and villus tissue of every abortion woman are respectively taken, pairing is formed by cDNA, the cDNA pairs are concentrated on the same chip, and the function presentation of parent-fetal interface of the physiological structure is achieved on the chip outside the body. The decidua and villus pairing cDNA chip has the advantages of being high in throughput, strong in specificity, high in sensitivity, simple and fast in operation, high in quantitative accuracy, strong in result reliability, simple in result analysis, complete in clinical information for samples and the like, and the cDNA chip can be used for quantitative detection of the expression statuses of a target gene in different patients and for conducting in-depth correlation analyses of clinical information.
Owner:夷希微医学科技(上海)有限公司
Application of leonurine in preparation of medicine for improving infertility pregnancy outcome
PendingCN111374967AImprove pregnancy rateWeaken invasive metastasisOrganic active ingredientsSexual disorderPhysiologyApoptosis
The invention belongs to the field of modern pharmacy of traditional Chinese medicines, and relates to application of a specific monomer leonurine in preparation of a medicine for improving infertility pregnancy outcome. The leonurine is injected into the abdominal cavity during an EMS (acute renal syndrome) modeling period, so that the volume and weight of an ectopic focus can be remarkably reduced, local fibrosis, invasion and transfer are inhibited, and the pregnancy rate of an EMS model mouse can be remarkably increased. The leonurine inhibits proliferation and invasion of endometrium stromal cells by inducing apoptosis of the endometrium stromal cells, adjusts expression balance of a local estrogen-progestin receptor of the ectopic focus, activates EMS model pelvic and abdominal immune cells, enhances phagocytic and killing functions of the cells, reverses poor ecdysis of the EMS model mouse, has similar effects on growth and invasion of ectopic focus ESC of a human EMS patient and expression of the estrogen-progestin receptor, does not affect growth and activity of the endometrium ESC of a normal child-bearing female, hormone receptor expression, ecdysis process and proliferation, apoptosis and cell activity of human early-pregnancy embryo trophoblast, and can be used for preparing an effective prevention and treatment medicine for EMS combined infertility patients.
Owner:THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
A method for preparing decidual mesenchymal stem cells
ActiveCN104560870BPollution prevention methodsReduced Chances of ContaminationSkeletal/connective tissue cellsSurface markerEnzyme digestion
Owner:江苏省北科生物科技有限公司 +1
Application of fructose 1,6-diphosphate in preparation of medicine for spontaneous abortion
ActiveCN108387722BOrganic active ingredientsIndividual particle analysisCell phenotypeRegulatory T cell
Owner:THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
MiRNA marker for diagnosing early abortion and application thereof
ActiveCN114438187ASuppressed expression levelEffective predictionMicrobiological testing/measurementDNA/RNA fragmentationFirst trimesterPregnancy early
The invention discloses a novel biomarker for early abortion diagnosis, and particularly relates to a method for diagnosing, predicting and / or monitoring early abortion by hsa-miR-29a-3p and application of hsa-miR-29a-3p. According to the invention, hsa-miR-29a-3p is proposed as a marker for early pregnancy embryo implantation and bad pregnancy outcome for the first time, and then the regulation effect of hsa-miR-29a-3p on decidualization is proved by using an in-vitro endometrial stromal cell decidualization model. The temporal-spatial expression specificity based on the marker has wide clinical diagnosis and prediction application prospects. According to the specific expression of the hsa-miR-29a-3p in different pregnancy periods and in decidua tissues of early abortion, the early abortion of pregnancy can be effectively predicted and diagnosed.
Owner:ATTACHED OBSTETRICS & GYNECOLOGY OSPITAL MEDICALCOLLEGE ZHEJIANG UNIV
A paired tissue chip of decidua and villi, its preparation method and its application
The invention relates to the technical field of biology, in particular to a tissue microarray of decidua and villi pairing, a preparation method and application thereof. The tissue microarray is prepared with the decidua and villus specimens of a normal pregnancy woman or a pathologic pregnancy patient arranged in sample application holes, so that pairing is formed between the decidua tissue and the villus tissue from the same specimen, and the functional presentation of the maternal-fetal interface of a physiological structure onto the tissue microarray is achieved. The tissue microarray has the advantages that high throughput is achieved, plenty of information is obtained in one time, and the efficiency is improved by hundreds of times; parallelization is achieved, the comparability is high, and the precision is high; the experimental error is small, and experimental conditions remain the same; convenience is brought to arrangement of various kinds of experimental control, and the research cost is greatly reduced. The tissue microarray can be used for etiology screening and molecular typing of pathologic pregnancy, screening and verifying of a novel marker, screening of individual treatment patients, judgment of a therapeutic effect and prognosis and the like, and the tissue microarray has important significance for the research of a normal pregnancy maintaining mechanism and pathologic pregnancy pathogenesis.
Owner:夷希微医学科技(上海)有限公司
A Simple Isolation and Identification Method of Human Placental Myofibroblasts
ActiveCN103173405BReduce usageAvoid introducingMicrobiological testing/measurementArtificially induced pluripotent cellsSurface markerDesmin
The present invention relates to a method for separating and identifying human placental myofibroblasts. After the placental fetal decidual tissue was mechanically disrupted, it was digested with DMEM digested solution containing collagenase I, and isolated into mononuclear cells. Cultivate under humidity to induce adherence. After 48 hours, change the medium in full to remove non-adherent cells and add fresh medium. After adherent cells form monoclonal cells, each monoclonal cell is cultured separately. When the cells are about 90% confluent, Trypsin passage to obtain myofibroblasts. The invention adopts the combined method of cell morphology, cell cycle, cell surface marker detection, cell adipogenic and osteogenic differentiation ability detection, cell pluripotent stem cell factor detection and cell desmin expression detection to identify the obtained myofibroblasts.
Owner:BEIJING JING MENG STEM CELL TECH
An obstetric cesarean section nursing tray structure with counting function
InactiveCN111544126BEffective physical isolationAvoid cross-contact infectionSurgical furnitureIntraoperative careNursing care
The invention discloses an obstetrical cesarean section nursing tray structure with an inventory function, which includes a chassis, a main tray and an inventory box are slidingly connected to both sides of the upper surface of the chassis, and a plurality of placement slots are opened inside the main tray , the bottom of the placement tank is made of a support plate, and there is a leakage hole in the middle of one end of the support plate close to the counting box, and the upper surface of the chassis is provided with waste liquid tanks corresponding to a plurality of placement tanks one by one. The instruments used in the operation are put into the placement groove in turn, and the instruments are supported by the support plate, which can effectively separate the instruments used respectively, avoiding cross-contact infection between the instruments, and avoiding the planting of active decidua tissue to the incision to prevent iatrogenic planting caused by the endometrium remaining in the incision.
Owner:郭晶
Tissue microarray of decidua and villi pairing, preparation method and application thereof
ActiveCN107192594AEasy to set upImprove accuracyPreparing sample for investigationEtiologyBiotechnology
The invention relates to the technical field of biology, in particular to a tissue microarray of decidua and villi pairing, a preparation method and application thereof. The tissue microarray is prepared with the decidua and villus specimens of a normal pregnancy woman or a pathologic pregnancy patient arranged in sample application holes, so that pairing is formed between the decidua tissue and the villus tissue from the same specimen, and the functional presentation of the maternal-fetal interface of a physiological structure onto the tissue microarray is achieved. The tissue microarray has the advantages that high throughput is achieved, plenty of information is obtained in one time, and the efficiency is improved by hundreds of times; parallelization is achieved, the comparability is high, and the precision is high; the experimental error is small, and experimental conditions remain the same; convenience is brought to arrangement of various kinds of experimental control, and the research cost is greatly reduced. The tissue microarray can be used for etiology screening and molecular typing of pathologic pregnancy, screening and verifying of a novel marker, screening of individual treatment patients, judgment of a therapeutic effect and prognosis and the like, and the tissue microarray has important significance for the research of a normal pregnancy maintaining mechanism and pathologic pregnancy pathogenesis.
Owner:夷希微医学科技(上海)有限公司
Preparation and cryopreservation method and application of human placental subchorionic large blood vessel tissue
ActiveCN109042624BGuaranteed survivalHigh activityDead animal preservationVascular tissueFresh Tissue
The invention discloses a method for preparing and freezing human placental subchorionic macrovascular tissue, comprising: (1) washing the placenta; cutting off the decidua along the edge of the placenta to remove the amniotic membrane; (2) removing the amniotic membrane from the placenta The fetal facial chorion and large blood vessels are separated together, washed with normal saline or PBS buffer, and the blood vessels are flushed to remove blood clots in the blood vessels; then, the large blood vessels connected to the chorionic plate are cut off one by one; (3) the blood vessels are transferred Put it into a cryopreservation tube or a cryopreservation bag, introduce the vitrification solution in a three-step method, transfer it to a programmed cooling device, cool it down to -80°C to -90°C, and transfer it to liquid nitrogen for cryopreservation. The cryopreservation method of the present invention is beneficial to improve the activity of cryopreserved tissues and cells, and the morphology, function, and structure after recovery are consistent with those of fresh tissues. The preserved tissues can not only be used in the fields of separating stem cells and epithelial cells, but also in tissue Engineering transplantation and other fields.
Owner:JIANGXI YINFENG DINGCHENG BIO ENG
Application of aspirin in preparation of medicine for preventing and treating unexplained recurrent abortion
PendingCN114053287ALow absorption rateReduces HMGB1 levelsOrganic active ingredientsDispersion deliveryRecurrent AbortionsAspirin
The invention relates to application of aspirin in preparation of a medicine for preventing and treating unexplained recurrent abortion. According to the invention, a small dose of aspirin is added into the drinking water of the unexplained recurrent abortion mouse, so that the embryo absorption rate is reduced, the inflammatory response of the decidua tissue of the mouse is improved, the HMGB1 level of the serum and the decidua tissue of the mouse is reduced, and the proinflammatory response further caused by the high-level HMGB1 of the unexplained recurrent abortion mouse is prevented; further, the pregnancy rate of the mice with unexplained recurrent abortion is effectively protected and treated.
Owner:THE FIRST AFFILIATED HOSPITAL OF ANHUI MEDICAL UNIV
Preparation and cryopreservation method and application of human placental amniotic membrane and decidua tissue
ActiveCN108812640BGuaranteed survivalHigh activityCulture processDead animal preservationFresh TissueAmniotic Sheet
The invention discloses a method for preparing and freezing human placental amniotic membrane and decidua tissue, comprising: (1) washing the placenta; cutting off the decidual membrane along the edge of the placenta, and dividing the amniotic membrane into tissue pieces; The amniotic membrane on the fetal surface of the placenta is removed from the attached placental tissue; (2) Wash the amniotic membrane and decidua with normal saline or PBS buffer solution, fold or lay the amniotic membrane and decidua into a cryopreservation bag or cryopreservation tube , add 4°C cryopreservation solution, seal, transfer to a programmed cooling device, cool down to -80°C according to the set cooling program, and transfer to liquid nitrogen for cryopreservation. The cryopreservation method of the present invention is beneficial to improve the activity of cryopreserved tissues and cells, and the shape, function and structure after recovery are consistent with fresh tissues, and the overall survival rate of cells in the tissues is over 90%, and the preserved tissues can not only be used for separation Stem cells, epithelial cells and other fields can also be used in fields such as tissue engineering and transplantation.
Owner:YINFENG BIOLOGICAL GRP
Preparation and cryopreservation method and application of human placental villous tissue
ActiveCN108812641BGuaranteed survivalHigh activityCulture processDead animal preservationFresh TissueGreat Blood Vessel
The invention discloses a method for preparing and freezing human placental villi, comprising: (1) washing the placenta; cutting off the decidua along the edge of the placenta to remove the amnion; (2) removing the placenta from which the amnion has been removed The fetal facial chorion and large blood vessels are separated together, and the remaining placental tissue is the placental villi tissue. Cut the placental villi tissue into small pieces, and then cut them into thin slices; put the thin slices into cryopreservation bags or tubes, and proceed in three steps. Introduce the vitrification solution by using the method, transfer to the program cooling device, cool down to -80°C to -90°C, and transfer to liquid nitrogen for cryopreservation. The cryopreservation method of the present invention is beneficial to improve the activity of cryopreserved tissues and cells, and the shape, function, and structure after recovery are consistent with those of fresh tissues, and the overall survival rate of cells in the tissues is over 90%, and the preserved tissues can not only be used to separate stem cells , epithelial cells and other fields, and can also be used in fields such as tissue engineering and transplantation.
Owner:YINFENG BIOLOGICAL GRP
Paired cDNA arrays for decidua and villi
ActiveCN107190328BRealize functional presentationImprove throughputMicrobiological testing/measurementLibrary creationClinical informationMedicine
The invention relates to a decidua and villus pairing cDNA chip. The cDNA chip is prepared through the following method that the decidua and villus tissue of a woman with early pregnancy abortion are taken, the RNA is extracted, cDNA is formed by reverse transcription and preset in a PCR reaction plate, and cDNA chip is made. A preparation method and application of cDNA chip are further provided. The method and application have the advantages that the decidua and villus tissue of every abortion woman are respectively taken, pairing is formed by cDNA, the cDNA pairs are concentrated on the same chip, and the function presentation of parent-fetal interface of the physiological structure is achieved on the chip outside the body. The decidua and villus pairing cDNA chip has the advantages of being high in throughput, strong in specificity, high in sensitivity, simple and fast in operation, high in quantitative accuracy, strong in result reliability, simple in result analysis, complete in clinical information for samples and the like, and the cDNA chip can be used for quantitative detection of the expression statuses of a target gene in different patients and for conducting in-depth correlation analyses of clinical information.
Owner:夷希微医学科技(上海)有限公司
Application of parasitic loranthus total polysaccharides in preparation of antiabortifacient
ActiveCN104288171AReduce vaginal bleedingHigh expressionOrganic active ingredientsSexual disorderPhysiologyReceptor
The invention discloses an application of parasitic loranthus total polysaccharides in preparation of an antiabortifacient. The parasitic loranthus total polysaccharides can promote pregnancy deciduas, and expression of estrogen and progestrone receptor of an ovary of an animal model, and can improve serum E2 and P level; secretion within the gestation period is adjusted; colporrhagia of the animal model is reduced; the abortion frequency is lowered; the abortion rate is lowered; gestation of rats is protected from multiple levels and factors; the miscarriage prevention effect is significant; and the curative effect is more excellent than those of parasitic loranthus total extracts.
Owner:罗颂平
Circular RNA hsa_circ_0001550 and application thereof
The invention discloses a circular RNA hsa_circ_0001550 and an application thereof. The nucleotide sequence of the annular RNA hsa_circ_0001550 is shown as SEQ ID No: 1, the hsa_circ_0001550 can participate in generation and development of RIF by inhibiting the periostracum periostracum process, meanwhile, the hsa_circ_0001550 plays a molecular function through miRNA and mRNA and participates in related signal channels in the endometrial interstitial cell deciduation process to adjust the receiving state of endometrium on an embryo in the implantation window period, thus, the circular RNA hsa_circ_0001550 can be used as a marker for detecting the abnormal state of deciduation of the female endometrial interstitial cells, or the circular RNA hsa_circ_0001550 has a good application prospect as a medicine for regulating and controlling the deciduation of the human endometrial interstitial cells.
Owner:THE FIRST HOSPITAL OF LANZHOU UNIV
Cellular therapeutic agent for incontinence or urine comprising stem cells originated from decidua or adipose
InactiveUS20130189232A1Preventing and urinary incontinenceBiocideMuscular disorderBacteriuriaLeak point pressure
Owner:RNL BIO
Embryo implantation detection kit, and application and use method thereof
The invention discloses an embryo implantation detection kit, and application and a use method thereof. The invention discloses application of a uterine decidua NK cell Siglec-7 as a detection index in an embryo detection kit. The uterine decidua NK cell Siglec-7 in a uterine decidua sample is detected as the detection index. The kit comprises a buffer solution, a fixing agent, a dehydrating agent, an embedding agent, a dewaxing agent, an antigen repairing agent, a fluorescent reagent, a primary antibody, a secondary antibody, a redyeing solution and a mounting medium. The detection tool provided by the invention is used for detecting the embryo implantation capability, and the endometrial embryo implantation capability can be further evaluated. A possible detection and clinical diagnosismode is provided for clinical unexplained spontaneous abortion female patients, and a powerful reference is provided for discussing unrevealed influence factors of the unexplained spontaneous abortionfemale patients.
Owner:THE WEST CHINA SECOND UNIV HOSPITAL OF SICHUAN
Method for obtaining decidua basalis mesenchymal stem cells and kit
InactiveCN106520688ACell dissociation methodsSkeletal/connective tissue cellsDigestion TreatmentMesenchymal stem cell
The invention discloses a method for obtaining decidua basalis mesenchymal stem cells and a kit. The method for obtaining the decidua basalis mesenchymal stem cells comprises the following steps: carrying out digestion treatment on decidua basalis by utilizing a mixed enzyme preparation, so as to obtain digested products; and separating the decidua basalis mesenchymal stem cells from the digested products, wherein the mixed enzyme preparation comprises pancreatic enzymes, collagenase IV and collagenase II. By adopting the method for obtaining the decidua basalis mesenchymal stem cells, large quantity of decidua basalis mesenchymal stem cells with higher purity and higher activity can be obtained.
Owner:SOUTH CHINA INSTITUDE OF BIOMEDICINE
Who we serve
- R&D Engineer
- R&D Manager
- IP Professional
Why Patsnap Eureka
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com