32 results about "Endometrial Stromal Cell" patented technology

The invention discloses a method for studying an action mechanism of Hsa_circ_0111659 in repairing of ESC by WJ-MSCs. According to the method, it is found that the hsa_circ_0111659 plays a positive regulatory role in repairing of injured stromal cells by the WJ-MSCs, and the proliferation of ESC can be reduced and the number of apoptotic cells in ESC can be increased by knocking down the hsa_circ_0111659. A targeting axis, namely the hsa_circ_0111659 / miR-20b-5p / VEGF plays an important role in repair of endometria, and the hsa_circ_0111659 provides a new idea for the repair of endometrial stromal cells.

The invention provides the use of decidual NK cells and cell subgroups in the preparation of drugs for treating infertility-related diseases. It has been confirmed by experiments that the decidual NK cells and their cell subsets can promote endometrial thickness increase, enhance endometrial cell viability, reduce endometrial cell damage, promote VEGF expression, maintain endometrial stromal cell stemness and stimulate proliferation. The method of treating endometrial growth disorder disease can increase the pregnancy success rate of endometrial injury model mice from 30% to 60-70%; The treatment of diseases related to maternal-fetal immune tolerance disorder at the level of lymphocytes provides a new way for the treatment of infertility.

The invention belongs to the field of protein detection, and in particular relates to a culture medium and a method for evaluating embryo quality by using the culture medium. The culture medium includes fetal bovine serum albumin, insulin-transferrin-selenium supplement and puromycin. The method for evaluating the quality of embryos by using culture medium in the present invention, through embryo culture and collection of embryo conditioned culture fluid, thawing embryo conditioned culture fluid, cultivating endometrial stromal cells, adding embryo conditioned culture fluid and protein detection, to obtain specific cells Embryonic quality can be assessed by differentially secreting proteins. The evaluation method of the invention is very effective and accurate, and fills up the gap in the market.

The present invention relates to the use of pyrroloquinoline quinine (PQQ), its derivatives and / or salts for the preparation of drugs for the treatment and / or prevention of endometriosis including adenomyosis, chocolate cyst of ovary, deeply infiltrating endometriosis and other type of endometriosis. In addition, the present invention relates to the use of PQQ in combination with one or more the following antioxidants: N-Acetyl-L-cysteine (NAC), resveratrol, epigallocatechin gallate, vitamin E or vitamin C for the preparation of drugs for inhibiting the proliferation, oxidative stress status, invasion and migration of endometrial stromal cell. The use of PQQ in combination with NAC shows a synergistic effect on inhibiting cell proliferation: significantly inhibiting the proliferation of endometrial stromal cell in vitro; significantly reducing the damaged size of tissue caused by endometriosis in vivo; significantly reducing the infiltration of endometrial stroma and glands; significantly reducing the dose of NAC as well. PQQ is beneficial for fertility, and increases the chances of getting pregnant. PQQ alone or PQQ in combination with NAC generally shows no side effects and will not interfere with pregnancy.