Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of sodium hydrosulfide serving as hydrogen sulfide donor in drug preparation

A technology of hydrogen sulfide donor and sodium hydrosulfide, which is applied in drug combination, sulfur/selenium/tellurium active ingredients, diseases, etc., and can solve the problems of anti-inflammation and anti-fibrosis in endometriosis models that have not yet been seen

Inactive Publication Date: 2017-10-24
THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV +1
View PDF2 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report that H2S can also play the role of anti-inflammation, anti-fibrosis, and inhibition of lesion growth and adhesion formation in endometriosis models

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of sodium hydrosulfide serving as hydrogen sulfide donor in drug preparation
  • Application of sodium hydrosulfide serving as hydrogen sulfide donor in drug preparation
  • Application of sodium hydrosulfide serving as hydrogen sulfide donor in drug preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Anti-IL-1β-induced inflammation of endometrial stromal cells by sodium hydrosulfide

[0036] 1. Cell viability determination is carried out in accordance with Dojindo's CCK8 manual. Take cells in logarithmic growth phase, trypsinize and pipette into a single cell suspension; inoculate a 96-well plate at a concentration of 5000 cells / well; wait for the cells After adherence (overnight) is stable, add serum-free NaHS (10-500μM) or SPRC (0.1-100μM) of different concentrations to continue the culture; select 6h, 12h, 24h, 36h, 48h time points, according to the medium of each well Add 10% of the total volume to the CCK-8 solution (be careful not to generate bubbles in the hole, so as not to affect the OD reading). At the same time, adding CCK8 to the cell-free medium is a blank control. 37℃5%CO 2 After 2h incubation in the incubator, the absorbance at 450nm wavelength was detected by the microplate reader.

[0037] 2. Flow cytometric detection of apoptosis is carried ...

Embodiment 2

[0045] Example 2 Sodium hydrosulfide reduces endometriosis and adhesions in a mouse model of endometriosis

[0046] 1. Autologous endometrial suture method mouse model

[0047] 1.1 Model establishment

[0048] Anesthetized with 1% sodium pentobarbital (0.1ml / 20g) by intraperitoneal injection, after the mice were anesthetized, the limbs were fixed with tape and fixed on the mouse board in the supine position. Shave the rat hair on the abdomen, disinfect with iodine, and deiodine with 75% ethanol. A 1cm midline incision was made in the lower abdomen to open the abdominal cavity and expose both uteruses. Separate one side of the uterus, ligate the upper and lower ends, free the middle section about 1-1.2cm, cut along the upper and lower ends and place in sterile normal saline, rinse gently to remove the fatty tissue and serosal surface, and move the uterine cavity along the longitudinal axis Cut open and divide them into 3-4 pieces. Each piece is about 3*3mm in size. A small segment ...

Embodiment 3

[0070] Example 3 The effect of sodium hydrosulfide in uterine horn adhesion model rats

[0071] 1. Establishment of rat uterine horn adhesion model: intraperitoneal injection of 7% chloral hydrate (0.4ml / 100g) anesthetize the rat, fix it on the rat board in the supine position, shave the lower abdomen rat hair, and disinfect with iodine. A midline incision in the rat's lower abdomen is about 3 cm. First, both sides of the abdomen wall are injured with a blade. The cardiomyocytes of SD rats are acutely separated: Maintain a uniform force and scrape up and down 10 times. Slight bleeding is appropriate. A 380 degree electric iron burns the bilateral uterine horns. At 9-10 locations, the uterine horns at both ends of the injury were sutured to the abdominal wall, one stitch on the upper and lower sides, with an interval of about 1 cm, the abdomen was closed in two layers, and the abdomen was opened for evaluation 14 days later. In the sham operation group, the operations were the sam...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the field of drug preparation, and relates to application of sodium hydrosulfide (NaHS) serving as a hydrogen sulfide donor in the drug preparation. The sodium hydrosulfide (NaHS) serving as the hydrogen sulfide donor is used for performing an experiment that H2S works on endometriosis and adhesion thereof through an endometrial stromal cell line, a C57 mouse endometriosis model and a Wistar rat uterine adhesion model. A result shows that the sodium hydrosulfide (NaHS) serving as the hydrogen sulfide donor can effectively inhibit IL-1beta induced endometrial mesenchymal cell inflammation; thermal hyperalgesia of an endometriosis model mouse can be obviously improved, so that lesion is reduced, fibrosis is relieved, and formation of postoperative adhesion of a uterine adhesion model rat can be inhibited; and the sodium hydrosulfide (NaHS) serving as the hydrogen sulfide donor can be further used for preparing a drug for treating the endometriosis and complications thereof.

Description

Technical field [0001] The invention belongs to the field of pharmaceutical pharmacy, and relates to the use of hydrogen sulfide donor sodium hydrosulfide (NaHS) in pharmacy, in particular to the preparation of hydrogen sulfide donor sodium hydrosulfide (NaHS) in the preparation of drugs for the treatment of endometriosis and adhesions thereof the use of. Background technique [0002] The prior art discloses that endometriosis (EMs) is a common disease in women of childbearing age, and its incidence rate is as high as 10-15% among women of childbearing age. The main clinical manifestations of this disease are pelvic pain and infertility. Affected women's quality of life. The pathogenesis of the disease has not yet been clarified, and clinical treatment practices are facing huge difficulties and challenges. The current interventions to remove lesions and anti-estrogen drug treatment can improve the condition and relieve symptoms to a certain extent, but it is still difficult to a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K33/04A61P15/00
CPCA61K33/04
Inventor 徐丛剑夏叶朱依谆焦杨吴志勇丛青王莹
Owner THE OBSTETRICS & GYNECOLOGY HOSPITAL OF FUDAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com