49 results about "Sodium pentobarbital" patented technology

The invention discloses a traditional Chinese medicine composition for assisting to improve sleep, a preparation method and application of the traditional Chinese medicine composition. The traditional Chinese medicine composition for assisting to improve sleep is prepared from, by weight, 15-65 parts of semen juglandis, 15-65 parts of spina date seed, 5-55 parts of salvia miltiorrhiza, 5-35 parts of acanthopanax, 5-35 parts of tuber fleeceflower stem, 5-15 parts of Astragalus membranaceus, 5-15 parts of lotus seed, 5-35 parts of jujube, 5-35 parts of wolfberry, 5-15 parts of sesame, 5-15 parts of grape skin, 5-15 parts of dark plum, and 5-15 parts of hawthorn. The animal experiment proves that the high and medium dosages of test materials can assist to prolong the sleep time of mice pentobarbital sodium and significantly shorten the sleep latent time of the mice pentobarbital sodium; however, every dosage is not directly acted on promoting animal sleep; therefore, the traditional Chinese medicine composition is promoted to be a health care product for improving human sleep; thus the life quality of the human body is obviously improved. The traditional Chinese medicine composition is applied to both of cold and warm figures, capable of eliminating and supplementing at the same time, compensating without obstructing the evil, giving consideration to Yin and Yang of liver, spleen and kidney, so that the dampness of spleen can be removed; moreover, the composition has the effects of sedative and hypnotic, promoting blood circulation to remove meridian obstruction, nourishing liver and kidney, and replenishing qi to invigorate the spleen.

The certified doctor in United States, use pentobabital sodium as the hypnotic to refrain central nervous system. Pentobabital sodium can be patience to the body after two weeks, and the effect decrease half, and have lingering effects and remain effects. The making method and pharmacological activity about the six kinds of plants and sodium pentobarbital's synergistic action wares are gaven patent. Under the cautious and strict conditions, extract the plant ingredients, absorb them by WLD resin, separate and purify them, and use the finger mark atlas quantity to make sure that the end product poise and stabilize. Six plant compound wares have sedate, hypnotize and synergy pentobabital sodium, anti-penfourzopol. The clinical use proved the reliability. It is safe and compensatory to the pentobabital sodium. It solves the hypnotic's tolerance and addiction. It solves the problem, which is expected to conquer for a long time.

The invention provides a milk beverage for regulating sleep disorder. The milk beverage comprises 1%-6.1% of blueberry juice powder, 0.5%-4.1% of whole milk powder, 1%-6.1% of gamma aminobutyric acid,0.1%-1.21% of spina date seed, 0.1%-1.21% of walnut peptide, 0.04%-0.41% of lily, 0.04%-0.41% of poria cocos, 0.04%-0.41% of double-petal red rose, 0.1%-1.21% of tea leaf theanine, 0.35%-2.62% of a stabilizing agent, 0.15%-0.502% of acidulant, 0.015%-0.151% of sweetening agent, 0.075%-0.251% of essence and the balance of water. According to the milk beverage, gamma-aminobutyric acid and medicinaland edible traditional Chinese medicines are taken as main components, so that in a hypnosis experiment of a pentobarbital sodium valve dosage, the sleep rate of a mouse is as high as 70%, and in anexperiment of prolonging the sleep time of pentobarbital sodium, the sleep time is as long as 42.13 min, so that the milk beverage has very good effects of regulating sleep disorder and helping sleep.

The invention relates to the technical field of health products and in particular relates to a composition for improving sleep and a preparation method thereof. The composition is characterized in that apple stem cell lyophilized powder, spina date seed extract and gamma-aminobutyric acid are compounded, the effect of obviously improving sleep is realized, and no toxic or side effect is produced. Experiments show that the effect for improving sleep of the provided composition is obviously better than the effects of respective components, and mouse acute toxicity test results show that abnormality is not found on a mouse and no poisoning or death phenomenon is caused; and the provided composition can effectively improve incidence that the mouse at subthreshold dose of pentobarbital sodium falls asleep at a dosage of 0.5g / kg.

The invention discloses applications of tupaia belangeri in preparation of an Alzheimer's disease model. The technical scheme is as follows: S1, an application of the tupaia belangeri, D-galactose, beta-amyloid protein1-42, ibotenic acid and pentobarbital sodium in preparation of the Alzheimer's disease animal model; S2, an application of the tupaia belangeri, D-galactose, beta-amyloid protein1-42, ibotenic acid and pentobarbital sodium in preparation of a tupaia belangeri disease model with at least one of following characteristics: 1), gliocyte proliferation; 2), neurofibril entanglement; 3), learning and memory ability deterioration.

The invention discloses a traditional Chinese medicine composition for treating insomnia, which is mainly prepared from the following raw material drugs in portion by weight: 5 to 15 portions of dactylicapnos root, 3 to 10 portions of grassleaf sweelflag rhizome, and 1 to 5 portions of Szechuan lovage rhizome. The invention also discloses a method for preparing crude drug powder, active component liquid medicine and dry extract powder of the traditional Chinese medicine composition. The traditional Chinese medicine composition has a simple formulation, and has the efficacies of tranquilizing and allaying excitement, activating blood and promoting Qi. Pharmacodynamics research results show that the traditional Chinese medicine composition applied by lavage can remarkably shorten the sleep latency of mice induced by sodium pentobarbital of threshold dosage, prolong sleep time, strengthen the hypnotic effects of the sodium pentobarbital of the threshold dosage, induce the mice given with the sodium pentobarbital of the sub-threshold dosage, and strengthen the hypnotic effects of the sodium pentobarbital of the sub-threshold dosage.

The invention relates to novel application of methylene blue to post-anesthesia awakening. The novel application is characterized in that after an animal is completely anesthetized, a certain dose of the methylene blue can be injected to the animal, so that awakening of the animal can be accelerated, the anesthesia time is shortened, and overdose anesthesia can be reduced to a certain extent; the novel application of the methylene blue is beneficial to relieving and resisting the overdose anesthesia by the aid of the methylene blue in animal experiments and clinical application; the experimental animal is anesthetized by the aid of pentobarbital sodium with certain concentration, then methylene blue solution with certain concentration is injected to the completely anesthetized animal, and the concentration of the methylene blue solution is lower than a toxic dose of the methylene blue; then the awakening time of the animal is observed, and an awakening effect of the methylene blue is judged after the awakening effect of the methylene blue is compared with an awakening effect of normal saline; as shown by experiment results, the required time for awakening the animal after the animal is anesthetized by the aid of the pentobarbital sodium can be obviously shortened by the aid of the methylene blue solution, an awakening latency stage can be shortened, and the overdose anesthesia can be reduced. The novel application of the methylene blue has the advantages that a safe, convenient and speedy awakening measure is hopefully provided for clinical application, and possible injury to patients due to the overdose anesthesia can be reduced.

The invention relates to an experimental method of [beta]-sitosterol for relieving breast fibrosis diseases. The experimental process is as follows: S1, selecting ICR mice 3-7 days after delivery as experimental subjects in the experiment, dividing the mice into three groups, namely a blank control group (NT), a mammary gland fibrosis model group (TGF-[beta]1) and an administration remission group (TGF-[beta]1+[beta]-stosterone), wherein each group comprises five mice; and S2, anesthetizing and fixing the mouse by using pentobarbital sodium, injecting TGF-[beta]1 into the fourth pair of milk regions of the mouse every three days to construct a breast fibrosis model, totally five cycles, and collecting a sample within 24 hours after the model is constructed. According to the experimental method of [beta]-sitosterol for relieving breast fibrosis diseases, the damage condition of breast tissue is evaluated through an H&E dyeing technology, the fibrosis condition of the breast tissue is evaluated through an Msaaon dyeing technology, and the expression quantity of Collagen II in the breast tissue is detected through adoption of a Westernblot method. Experimental results can prove that the [beta]-sitosterol has an effect of relieving breast fibrosis.

The present invention relates to the technical field of health products, particularly to a sleep improving composition and a preparation method thereof. According to the present invention, apple stem cell freeze-drying powder, melatonin and vitamin B6 are compounded so as to provide characteristics of significant sleep improvement function and no toxic-side effect; experiment results show that the sleep improving effect of the composition of the present invention is significantly superior to the sleep improving effect of the single use of each component, and mouse acute toxicity test results show that the mouse does not present abnormality and no poisoning or death occur; and the falling-asleep occurrence rate of the mice with the sub-threshold dose of sodium pentobarbital can be effectively improved under the composition dose of 0.5 g / kg.

A novel euthanasia composition for animals, especially companion animals, utilizes a combination of sodium pentobarbital with dibucaine. The composition is easy to administer, fast-acting and relatively painless. A humane method for euthanasia involves administering the composition to animals for which euthanasia is desired.

The invention discloses an experimental method for treating mastitis by using pedunculoside. The experimental method specifically comprises the following steps: S1, selecting ICR mice 3-7 days after childbirth as experimental objects in the experiment; and dividing the ICR mice into five groups, namely a blank control group (NT), an administration group (PE), a mastitis model group (LPS), a drug therapy group (LPS+PE) and a dexamethasone positive control group (LPS+DEX), wherein each group comprises 9 mice; and S2, anesthetizing and fixing the mice by using pentobarbital sodium (45mg / kg), injecting LPS (0.2 mg / kg) into a fourth pair of nipples of the mice to construct a mastitis model, and collecting a sample after 24 hours. The invention discloses an experimental method for treating mastitis by using pedunculoside. Pathological damage conditions of mammary gland tissues are detected by a paraffin section method, expression conditions of inflammatory genes IL-1[beta] in the mammary gland tissues are evaluated by a fluorescent quantitative PCR technology, and experimental results show that pedunculoside can effectively treat LPS-induced mastitis.

The invention discloses a composition capable of calming mind and assisting sleep, a preparation method therefor and application of the composition. The composition capable of calming the mind and assisting the sleep comprises raw oyster shells, wild jujube seeds, lily bulbs, poria cocos, dried red dates, roses, fried malt, fructus crataegi, radix glycyrrhizae, dried tangerine peels, fresh oyster meat, mussels and gamma-aminobutyric acid. The composition capable of calming the mind and assisting the sleep is natural in ingredient, scientific in proportioning ratio and free of toxic or side effects; and confirmed by animal in-vivo experiments, the composition can be used for obviously increasing sleep incidence rate of sodium pentobarbital in a sub-threshold dose and shortening the sleep latency period of sodium barbital, plays a role in improving the sleep, and then, can be applied to preparation of foods or healthcare products for improving the sleep.

The present invention relates to a pentobarbital formulation with greater stability and fewer impurities. In particular, the formulation may be an aqueous formulation containing 50 mg / mL pentobarbital sodium, 50% glycol, and 10% alcohol, at a pH of 9.4.

The invention discloses a health-care food for improving sleep. The health-care food for improving sleep is characterized by being prepared from the following raw materials in parts by weight: 20-40 parts of fructus ziziphi jujubae, 20-40 parts of wheat, 20-40 parts of lilies, 10-30 parts of radix rehmanniae recens and 5-15 parts of plumula nelumbinis. The health-care food for improving sleep disclosed by the invention has the functions of nourishing heart and soothing the nerves and improving sleep and is suitable for crowds with sleep disorders induced by persistent mental stress and crowds in abnormal emotions such as being in low spirits and being restless and the like induced by the sleep disorders to eat. Results of mouse direct sleep experiment, pentobarbital sodium sleep time-prolonging experiment, pentobarbital sodium subthreshold dose sedative experiment, pentobarbital sodium sleep incubation period experiment, rate anxiety-depression experiment and the like verify that the health-care food can be used for remarkably alleviating and adjusting the sleep disorders and the abnormal emotions such as being in low spirits and being restless and the like induced by the sleep disorders.

The invention relates to a composition with a sleep improving function and application. The composition with the sleep improving function is prepared from the following components in parts by weight: 10 to 40 parts of cannabidiol, 20 to 80 parts of rhizoma polygonati extract and 60 to 150 parts of human milk oligosaccharide. The cannabidiol, the rhizoma polygonati extract and the human milk oligosaccharide are taken as main raw materials, the three components are reasonably combined, the cannabidiol can relieve pressure and resist anxiety, the rhizoma polygonati can tonify deficiency and nourish yin, and the human milk oligosaccharide can nourish cranial nerves; the three components are matched and used, supplement each other and synergistically play a role in improving sleep. Animal experiment results prove that after the composition is orally taken to a mouse for 30 days, experiment results of prolonging the pentobarbital sodium sleep time and experiment of pentobarbital sodium subthreshold hypnotic dose are positive, and no obvious direct sleep effect exists, so that the composition has the effect of improving the sleep function, and the composition can be used for preventing and treating the sleep. Meanwhile, side effects on human bodies are avoided, and the product safety is high.

The invention relates to an experimental method for treating mastitis diseases by using a fagopyridine extract. The fagopyridine extract contains 53.6% of rutin and 37.2% of quercetin. The experimental process is as follows: S1, selecting ICR mice 3-7 days after delivery as experimental subjects in the experiment, dividing the mice into three groups, namely a blank control group (NT), a mastitis model group (LPS) and an administration treatment group (LPS + TBF), and each group comprising three mice; and S2, anesthetizing and fixing the mice by using pentobarbital sodium, injecting LPS into the fourth pair of milk areas of the mice in the mastitis model group and the administration treatment group to construct a mastitis model, and taking a sample after 24 hours. According to the experimental method for treating mastitis by using a fagopyrine extract, the damage condition of the gland tissue is evaluated by using an H&E dyeing technology, and the activity of myeloperoxidase (MPO) in the mammary gland tissue is detected. Experimental results prove that the fagopyrine extract has a treatment effect on mastitis.

The invention relates to the technical field of chemical engineering and biological medicine, in particular to an adsorbent for removing protein binding uremic toxin through blood perfusion and a preparation method thereof. The adsorbent for removing the protein-bound uremic toxin through blood perfusion is porous resin which has an amide group and takes polystyrene-acrylonitrile-divinylbenzene as a skeleton, and the porous resin has imprinted holes of imprinted molecules; and the imprinting molecules include a protein-binding toxoid and / or an analog of a protein-binding toxoid. The adsorbent disclosed by the invention has a remarkable removal effect on protein binding toxoid such as indoxyl sulfate and p-cresol sulfate, also has a certain removal capability on middle-macromolecule and small-molecule substances such as beta 2-microglobulin, vitamin B12, creatinine and pentobarbital sodium, and is excellent in safety performance and mechanical strength.

The invention belongs to veterinary medicine preparation technology and provides a compound anesthetic for cats and a preparation method thereof. The compound anesthetic is formed by ketamine hydrochloride, dexmedetomidine, tramadol hydrochloride, pentobarbital sodium, sodium chloride, gelatin and water for injection. The ketamine hydrochloride, the dexmedetomidine, the tramadol hydrochloride, the pentobarbital sodium, the sodium chloride and the gelatin are dissolved in the water for injection in sequence, by means of 0.2 mole per litre of hydrochloric acid solution, potential of hydrogen (PH) is adjusted to 7.8, the water for injection is added again, after dilution and filtering, the obtained solution is poured into an ampoule bottle, and after sterilization, the compound anesthetic for the cats can be obtained. The compound anesthetic is rapid in anesthesia induction, good in anesthetic effects, safe and effective in the process of anesthesia, slight in influence on normal physiological and biochemical indexes, small in toxic and side effects, and the cats can revive stably, and the preparation method is simple and feasible.

The invention discloses a VX2 rabbit liver transplantation tumor model. A construction method of the VX2 rabbit liver transplantation tumor model comprises the steps as follows: firstly, an anesthetic for intramuscular injection is prepared, the concentration of the anesthetic is 3% pentobarbital sodium, and the dosage is 1 mL / kg; then the VX2 tumor block for intramuscular injection is processed into a tumor block with the size of 1*1*1 mm<3>, the tumor block is inoculated into the body of a young rabbit in the anesthetic state for tumor formation, and the tumor formation parts are rabbit thigh muscle parts on the two sides of the young rabbit; and finally, separated VX2 tumor tissue is rapidly stored in PBS (Phosphate Buffer Solution) at 4 DEG C, muscular tissues around the tumor are removed by using a cell operation table, the tumor block is cut into 1*1*1 mm<3>, and the tumor block is transplanted into the liver of a healthy adult rabbit for planting. The VX2 rabbit liver transplantation tumor model is high in tumor formation rate, a formed tumor is a single tumor generally, the probability of abdominal cavity implantation metastasis and abdominal cavity infection is low, and follow-up experimental research is facilitated.

The invention discloses compound injection for white raccoon dogs and a method for preparing the compound injection. The compound injection and the method have the advantages that three medicines including tasimelteon, pentobarbital sodium and androctonus bicolor polypeptides are scientifically and reasonably compatible with one another, and accordingly the shortcomings of existing anesthetics canbe overcome under synergistic effects of the various medicines.

The invention discloses a preparation method of a rat corneal limbus stem cell deficiency model in the field of corneal epithelium. The method comprises the following steps of S1, anesthetizing an experimental rat by adopting pentobarbital sodium intraperitoneal injection, and dripping 4 mg / mL of procaine into the experimental eye of the rat for local anesthesia; S2, by utilizing conical filter paper, infiltrating all parts of the corneal limbus of the rat at the same time, and controlling the placement time of the filter paper more accurately; and S3, soaking the conical filter paper prepared in the step S2 in a sodium hydroxide solution, clamping the top of the conical filter paper through tweezers, and slightly sucking redundant liquid on the conical filter paper through gauze. By utilizing the conical filter paper, all the parts of the corneal limbus of the rat can be infiltrated at the same time, and the placement time of the filter paper can be more accurately controlled; compared with a traditional plane filter paper method, the preparation method has the advantages that the rat corneal limbus stem cell deficiency can be more stably and reliably induced, a good animal model for simulating a clinical patient is provided for experimental research, and a foundation is laid for further therapeutic research.

The invention relates to the technical field of biological and medical technologies, in particular to a rat infrarenal abdominal aortic aneurysm model constructed through a retroperitoneal approach and a construction method. The operation is relatively simple, risks caused by the abdominal approach can be avoided, pressurized perfusion is not adopted, the perfusion time is shortened to 20 min, lower limb ischemia does not occur, the tumor formation rate is not affected, damage to blood vessels is extremely small, bleeding can be stopped through local compression after perfusion is finished, and the operation success rate is remarkably increased; The construction method comprises the following steps: S1, performing fasting for 12 hours before an operation of a rat, anesthetizing the rat with pentobarbital sodium according to 40-50mg / kg, taking a right side lying position after satisfaction of anesthesia, conventionally preserving skin, performing disinfecting, taking a connecting line from the lower part of a left side posterior axillary line costal margin to the root of a thigh as an incision with the length of 3-3.5 cm, cutting the skin, subcutaneous tissue and muscles layer by layer to see the retroperitoneal fat tissue, finding a potential gap between the peritoneum and lumbar dorsal muscles along the fat tissue, separating the gap by a cotton swab to reach the aorta abdominalis, and separating the aorta abdominalis from the inferior vena cava.

The invention relates to a composition and application with the function of improving sleep. The composition with the function of improving sleep comprises the following components in parts by weight: 10-40 parts of cannabidiol, 20-80 parts of Rhizoma Polygonatum extract, and 60-150 parts of human milk oligosaccharide. The present invention uses cannabidiol, Polygonatum extract and human milk oligosaccharides as main raw materials to make a reasonable prescription of the three ingredients, cannabidiol relieves stress and relieves anxiety, Polygonatum nourishes deficiency and nourishes yin, and human milk oligosaccharides nourishes the cranial nerves; three Those who cooperate and share, complement each other, and play synergistically to improve sleep. Confirmed by animal experiment results: after oral administration to mice of the composition of the present invention for 30 days, the experiment of prolonging the sleep time of pentobarbital sodium and the subthreshold hypnotic dosage experiment of pentobarbital sodium were carried out, and the experimental results were all positive, and there was no obvious The direct sleep effect shows that the composition of the present invention has the effect of improving sleep function, has no side effects on the human body, and has high product safety.

The invention belongs to the technical field of pancreatic cancer research, and in particular relates to a method of inhibiting pancreatic cancer development in mice by apolipoprotein AI mimetic peptide L-4F. Aiming at the problem that the research method for inhibiting the development of pancreatic cancer has high cost and no obvious effect, the following scheme is proposed, including the following steps: establishing a mouse model: C57BL / 6 mice are subjected to fasting for 24 hours before operation and can drink water; 2% sodium pentobarbital (1.5 mL / kg body weight) is injected intraperitoneally to anesthetize animals in each group; for all animals in each group, sodium pentobarbital is injected into the abdomen through a 1.5 cm median incision of the upper abdomen; after pancreas of thepancreatic cancer model group is exposed, apolipoprotein AI mimetic peptide L-4F suspension is injected into the tail of pancreas through a 1mL syringe and the mice are raised in common environment;in 3-5 months, the mental state, body weight and ascites of mice are observed at any time. According to the invention, the mouse model is more stable in establishment, the research cost is saved, theobservation is more intuitive, the multi-parameter experiment is carried out, and the accuracy of the experimental research is improved.

The invention discloses application of flos daturae in cooperation with pentobarbital sodium in preparation of a medicine for improving sleep, and belongs to the technical field of medicine products, and the mass ratio of flos daturae to pentobarbital sodium is 1: 1 or 1: 16.5-32.5. Mouse experiment research finds that the flos daturae has an effect of inhibiting autonomous activity of mice but does not have a direct sleep effect when being independently used, but synergistic effects of the flos daturae and the pentobarbital sodium of the subthreshold dosage and the pentobarbital sodium of the suprathreshold dosage find that the flos daturae and the pentobarbital sodium of the subthreshold dosage can significantly improve the sleep rate of the mice; meanwhile, the flos daturae and the pentobarbital sodium in a suprathreshold dose can remarkably shorten the sleep latency period of the mouse and prolong the sleep time, namely, the flos daturae and the pentobarbital sodium generate a synergistic effect, can inhibit a central nervous system and have sedative and hypnotic effects, so that the flos daturae and the pentobarbital sodium can be used for preparing the medicine for improving the sleep, have no obvious side effects and are suitable for clinical application. Wide application prospects are realized.

The invention provides an application of an ilextrotunda thunb extract in preparation of a drug for promoting sleep. The Ilextrotunda thumb is used as a pharmaceutical plant with a wide range of medicinal uses, its extracts, especially a total saponin extract, more preferably, the pedunculoside in the total saponin extract can significantly improve insomnia caused by pentobarbital sodium in aspectof sleep promotion, can you shorten the sleep time, but can also extend the sleep time. The present application provides a novel purpose of the ilextrotunda thunb extract in promoting sleep, and provides a new selection for the current preparation of a sleep-promoting drug.

The invention relates to the technical field of plant extracts, in particular to a composition containing a picea melanocarpa extract and a sleep-aiding product. The invention discloses a composition. According to the sleep-aiding composition, a spruce schefflera extract, vetiver essential oil and real lavender essential oil are matched with one another, so that the sleep latency duration of a mouse in a pentobarbital sodium induced sleep experiment can be shortened, and the sleep time can be prolonged; the number of mouse hippocampal neurons is increased; the expression quantity of 5HT-1A protein and GABAAR[alpha]1 protein in each region of the brain is improved. The sleep-aiding composition has a remarkable sleep-aiding effect.

The invention provides a corneal neovascularization animal model and a construction method thereof, and the animal model is constructed by making a sneak tunnel right in front of the corneal limbus at an incision in the center of the cornea of a mouse and implanting filter paper containing vascular endothelial growth factors into the top end of the tunnel, thereby obtaining the corneal neovascularization animal model. Before the central incision of the cornea of the mouse, the pentobarbital sodium solution is injected into the abdominal cavity of the mouse, and the Elkheline eye drops anesthetize the surfaces of the two eyes of the mouse; the filter paper is thin, short in manufacturing time, easy to implant in the operation process and high in molding success rate, so that the repeatability of molding is improved, and the cost investment is greatly reduced; the filter paper is combined with the vascular growth factor to be implanted into the mouse cornea interlayer to induce the mouse cornea neovascularization model, and the mouse cornea neovascularization model can be used for the mouse cornea, has the characteristics of short modeling time, low cost, small trauma and high repeatability, and provides a stable and reliable model system for researching the cornea neovascularization inhibitor.