Experimental method for treating mastitis disease by using fagopyrine extract

Abstract

The invention relates to an experimental method for treating mastitis diseases by using a fagopyridine extract. The fagopyridine extract contains 53.6% of rutin and 37.2% of quercetin. The experimental process is as follows: S1, selecting ICR mice 3-7 days after delivery as experimental subjects in the experiment, dividing the mice into three groups, namely a blank control group (NT), a mastitis model group (LPS) and an administration treatment group (LPS + TBF), and each group comprising three mice; and S2, anesthetizing and fixing the mice by using pentobarbital sodium, injecting LPS into the fourth pair of milk areas of the mice in the mastitis model group and the administration treatment group to construct a mastitis model, and taking a sample after 24 hours. According to the experimental method for treating mastitis by using a fagopyrine extract, the damage condition of the gland tissue is evaluated by using an H&E dyeing technology, and the activity of myeloperoxidase (MPO) in the mammary gland tissue is detected. Experimental results prove that the fagopyrine extract has a treatment effect on mastitis.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an experimental method for treating mastitis with a buckwheat alkali extract. Background technique [0002] Cow mastitis is an inflammation of the udder parenchyma and interstitium. The etiology is mostly caused by mechanical stimulation, invasion of pathogenic microorganisms and chemical and physical damage. The main symptoms are redness, swelling, heat, pain and other inflammatory manifestations of the breast. At the same time, it is accompanied by symptoms such as reduced or stopped lactation, changes in milk composition, and reduced milk production. The prevention and treatment of dairy cow mastitis causes huge economic losses to animal husbandry every year. [0003] Among the many pathogenic factors causing mastitis, the invasion of pathogenic microorganisms is the main cause. At present, about 150 kinds of pathogens have been found to cause mastitis in cows, among which ...

AI Technical Summary

Problems solved by technology

[0006] According to the current deficiencies in the treatment of mastitis, the present invention provides an experimental method for the treatment of mastitis with a buckwheat alkali extract. The present invention has medicinal extraction materials that are harmless and have a wide range of sources, and have obvious effects in treating mastitis. It is beneficial to solve the problems of antibiotic residues, the emergence of drug-resistant bacteria and the shortage of alternative therapeutic drugs during the treatment of dairy cow mastitis

Images