91 results about "Creatinine" patented technology

The present disclosure relates to an improved enzyme sensor comprising a cover membrane layer of a porous polymeric material, the outer surface and pore mouths of at least one face of the porous polymeric material being covered by a hydrophilic polymer. The sensor is useful determining the presence or amount of biological analytes, e.g., glucose, lactate, creatine, creatinine, etc.

The invention is a cosmetic or dermatological preparation with a content of an active agent combination comprising (a) at least one creatinine compound selected from the group consisting of creatinine and derivatives of creatinine; (b) at least one creatine compound selected from the group consisting of creatine and derivatives of creatine; and (c) at least one soybean compound selected from the group consisting of soybean germ extracts and ingredients which can be isolated from soybean germ. The present invention also includes methods for stimulating collagen synthesis of the skin, for restructuring or rejuvenation of the skin, and for inspiring the body or senses comprising applying the preparation to the skin.

A combination comprising creatine and / or creatinine and / or a derivative thereof and one or more hydrocolloids. This Abstract is not intended to define the invention disclosed in the specification, nor intended to limit the scope of the invention in any way.

A stable pharmaceutical formulation is provided that comprises a biologically active protein and an excipient selected from carnitine, creatine or creatinine.

Personal care composition including a first skin and / or hair care active cetyl pyridinium chloride; and at least one additional skin and / or hair care active selected from the group consisting of tetrahydrocurcumin, sugar amine, vitamin B3, retinoids, hydroquinone, peptides, phytosterol, dialkanoyl hydroxyproline, hexamidine, salicylic acid, n-acyl amino acid compounds, sunscreen actives, water soluble vitamins, oil soluble vitamins, hesperedin, mustard seed extract, glycyrrhizic acid, glycyrrhetinic acid, carnosine, Butylated Hydroxytoluene (BHT) and Butylated Hydroxyanisole (BHA), ergothioneine, vanillin or its derivatives, diethylhexyl syrinylidene malonate, melanostatine, sterol esters, idebenone, dehydroacetic acid, Licohalcone A, creatine, creatinine, feverfew extract, yeast extract, beta glucans, alpha glucans, their salts, their derivatives, their precursors, and / or combinations thereof; and a dermatologically acceptable carrier. The invention further relates to methods for regulating the condition of mammalian keratinous tissue wherein the methods each comprise the step of topically applying to the keratinous tissue of a mammal needing such treatment, a safe and effective amount of the personal care composition of the invention.

The invention relates to a composition for eliminating the interference of a calcium dobesilate medicine on creatininase-method detection. The composition comprises an R1 reagent, an R2 reagent and ahigh molecular oxidant, wherein the high molecular oxidant is one or more of high molecular peroxy acid, high molecular selenium oxide, high molecular sulfur chloride ether, N-substituted imide, water-soluble tetrazole and a Dess-Martin oxidant. The high molecular oxidant in the composition disclosed by the invention can react with the calcium dobesilate in a sample to be detected, the calcium dobesilate loses the effect of interfering enzyme-method creatinine detection and the accuracy of sarcosine-oxidase-method detection is improved, so that the problem of serious negative interference, caused by high-concentration calcium dobesilate in blood of a patient, on an enzyme-method creatinine project detection result when the patient orally takes the calcium dobesilate medicine is solved.

The invention discloses a storage tolerance cell cryopreservation liquid which comprises propylene glycol, dextran, potassium chloride, magnesium chloride, disodium hydrogen phosphate, monopotassium phosphate, sodium chloride, vitamin C, cholesterol, sodium bicarbonate, albumin, glucose, creatinine, chloramphenicol, streptomycin, glycerinum, sodium carboxymethylcellulose and sterile injection water. The components of the storage tolerance cell cryopreservation liquid disclosed by the invention are coordinated with one another and act with one another, so that the cryopreservation liquid disclosed by the invention can be easily preserved and has a relatively long shelf life, that is, the shelf life is as long as 3 months or grater, the operation is simple, the cryopreservation liquid does not need to be prepared temporarily on site, the time and the labor are saved, the cryopreservation liquid can be preserved at low temperature directly, no complex cryopreservation procedure is needed, the cell cryopreservation time is greatly shortened, the cryopreservation efficiency is improved, and the cryopreservation liquid is stable and reliable in preservation effect, convenient to transport, convenient to use, simple and easily available in raw material and worthy of market popularization and application.

The invention discloses a method for preparing high-content creatine phosphate disodium salt, comprising the steps as follows: (1) conducting a condensation reaction on creatinine and phosphorus oxytrichloride to obtain crude creatinine phosphorus oxychloride; (2) dissolving the crude creatinine phosphorus oxychloride in a solvent A, then stirring and filtering, adding a solvent B to the filtrate to separate out the crystal and obtain fine creatinine phosphorus oxychloride; (3) hydrolyzing fine creatinine phosphorus oxychloride in sodium hydroxide aqueous solution to obtain the high-content creatine phosphate disodium salt, wherein the solvent A is halohydrocarbon and the solvent B is aliphatic hydrocarbon.

The present invention relates to a catalytic synthesis method of medical biodegradable polyester. Said invention adopts carboxylic creatinie quanidine compound whose structure formula is provided by said invention to calalyze bulk activity ring-opening polymerization reaction of L-lactide (LLA) or D,L-lactide (DLLA) or (3S)-3-benzyloxymethyl-(6S)-6-methyl-morpholine diketone (serine morpholine diketone)(BMD) so as to synthesize poly-L-lactide (PLLA), poly-D,L-lactide (PDLLA) and polyserine morpholine diketone (PBMD). Said polymer has high biological safety, the PBMD can be used for synthesizing L-serine-L-lactic acid alternate copolymer (PCLSE-LLA).

The present invention provides amide-protected creatine molecules and compositions, containing one or more bioactive forms of creatine in aqueous compositions, wherein bioactive forms of creatine do not appreciably degrade into creatinine. Also provided are various beneficial effects of administering aqueous compositions having at least one amide-protected creatine molecule.

The invention relates to a new method for synthesis of a medical biodegradable material poly lactic acid-glycolic acid by copolycondensation of lactic acid and glycolic acid through a catalysis effect of a bionic creatinine hydrochloride creatininium chloride (creatininium hydrochloride). According to the method, the bionic creatininium chloride is adopted as a catalyst, the industrial-grade lactic acid (LA, 85% aqueous solution) and the glycolic acid (GA, 95%) are adopted as monomers, and bulk solvent-free two-stage copolycondensation is performed to obtain the medical degradable poly lactic acid-glycolic acid with characteristics of high biological safety, no toxicity and no metal. According to the present invention, the method of the present invention has characteristics of green catalyst and solvent-free bulk polymerization; the catalyst creatininium chloride has high biocompatibility and high biological safety, and does not have cell toxicity; the synthesized poly lactic acid-glycolic acid does not contain any metals and other toxic residues, and is applicable for medical and medicinal materials; the process is simple and easy to operate, the raw material cost is low, the operation is simple, and the method is applicable for the industrial implementation.

The invention discloses a nested PCR (polymerase chain reaction) kit for detecting chicken-manure pollution in a water body and a detection method thereof, and belongs to the field of the detection of pollution in water bodies. The nested PCR kit for detecting the chicken-manure pollution in the water body comprises two pairs of nested PCR primers, dNTP (deoxyribonucleoside triphosphate), a PCR buffer, a Taq polymerase, a Mg<2+> solution and ddH2O (double distilled water). Two rounds of PCR amplification are used by a nested PCR method. The detection method comprises the following steps of extracting a DNA (deoxyribonucleic acid) in a water sample, and carrying out a first round of PCR amplification by using the DNA in the water sample as a template, wherein the primers are an SCF (stem cell factor) and SCR (serum creatinine); after a reaction is terminated, carrying out a second round of PCR amplification by using a product of the first round of PCR amplification as a template, wherein the primers are an NCF (neutrophil chemotactic factor) and NCR (nitrile chloroprene rubber), and after the second round of PCR amplification is terminated, detecting the existence of a 403bp strip, which shows that the water body is subjected to the chicken-manure pollution, through agarose gel electrophoresis. According to the method, the detection sensitivity is greatly increased through the nested PCR amplification; a little chicken-manure pollution which exists in water can be detected; moreover, the method has a favorable specificity, and can be directly applied to the detection and the preventive treatment work of fecal pollution in the water body.

Compositions containing one or more bioactive forms of creatine which are aqueous compositions in which the one or more bioactive forms of creatine do not appreciably degrade into creatinine. Also are methods for providing various beneficial effects which comprise administering aqueous compositions comprising at least one creating O-phosphate species to a mammalian subject, either chronically or acutely.

A combination comprising creatine and / or creatinine and / or a derivative thereof and one or more retinoids. This Abstract is not intended to define the invention disclosed in the specification, nor intended to limit the scope of the invention in any way.

The disclosure provides for a lab-on-a-chip (LOC) device and a method of fabrication thereof. Additionally, a system and a method for point of care testing of multiple biomarkers such as glucose, cholesterol, creatinine, uric acid, and bilirubin is provided. The microfluidic assembly consists of three layers in which the top and the middle layers are made up of polydimethylsiloxane (PDMS) and the bottom layer with polyethylene terephthalate (PET). The device integrates screen printed non-enzymatic electrochemical sensors in the bottom layer for simultaneous detection of glucose, cholesterol, creatinine, uric acid, and bilirubin. A hand held potentiostat with readout enables readout for the point of care application of integrated sensing device. The device developed has potential to revamp healthcare by providing access to affordable technology for better management a diabetes and related complications at every door step.

A creatine amidinohydrolase having the following physicochemical properties: Action: catalyzing the following reaction; creatine + H2O -> sarcosine + urea Optimum temperature: about 40 - 50 DEG C Optimum pH: pH about 8.0 - 9.0 Heat stability: not more than about 50 DEG C (pH 7.5, 30 min) Km value for creatine in a coupling assay using a sarcosine oxidase and a peroxidase: about 3.5 - 10.0 mM Molecular weight: about 43,000 (SDS-PAGE) Isoelectric point: about 3.5, a method for producing said enzyme, comprising culture of microorganism producing said enzyme, a method for the determination of creatine or creatinine in a sample using said enzyme, and a reagent therefor. According to the present invention, a creatine amidinohydrolase having a smaller Km value than that of the conventionally known creatine amidinohydrolase can be produced in an industrially large amount, and can be used as a routine reagent for clinical tests for determining creatine and creatinine in biological samples.

The present invention relates to a sensor for detecting one or more target analytes, the sensor comprising: at least one polymeric sensing element capable of selectively and reversibly binding to a target analyte; at least one working electrode having the polymeric sensing element disposed thereon; at least one reference electrode that is electrically communicated with said working electrode; and means for measuring a change in an electrical property across said working electrode and said reference electrode. In particular, the target analyte is Na+, urea or creatinine. Also disclosed is a multi-layered sensor, comprising at least one working electrode layer and at least one reference electrode layer, said working electrode layer and said reference electrode layer being separated by at least one electrically insulating layer.

The invention discloses a kit and method for measuring creatinine. The kit comprises a first reagent, and the first reagent is prepared from monopotassium phosphate, dipotassium phosphate, ethylenediamine tetraacetic acid, Brij 35, Tween 80, ascorbic acid oxidase, creatinase, sarcosine oxidase, TOOS, peroxidase, potassium ferrocyanide, gentamicin sulphate and Tween 30. The method comprises the following steps: (1) the first reagent and a second reagent are dissolved according to the raw material components and content; (2) the dissolved first reagent is added into a to-be-measured sample; (3)the dissolved second reagent is added; and (4) the absorptivity of a solution under the wavelength of 546 nm is measured. According to the kit and method for measuring the creatinine, the precision and accuracy are high, and stability is achieved.

The invention discloses a micromolecular marker for the diagnosis of malignant pleural effusion and an application thereof, wherein the micromolecular marker comprises the following 11 compounds of: valine, lactate, alanine salt, acetacetate, creatinine, trimethyl-amine oxide, betaine, alpha- and beta-glucose, leucine and isoleucine. In allusion to the components of pleural effusion of cancer patients and the content variation of the components, the micromolecular marker, which has strong specificity, high sensitivity and high appraisal result accuracy and is used for the diagnosis of malignant pleural effusion, is selected by the combination of the nuclear magnetic resonance technology and the stepwise discriminant function analysis method; in particular, lactate, acetacetate, trimethyl-amine oxide, betaine and alpha-glucose can sensitively, accurately and efficiently identify benign and malignant pleural effusion, which is of great significance for accurately diagnosing malignant pleural effusion such as lung cancer at an early stage, striving for much more treatment time for patients and raising the clinic treatment effect.

The present invention is based on the finding that enrichment of isotope-labeled creatinine in a urine sample following oral administration of a single defined dose of isotope-labeled can be used to calculate total-body creatine pool size and total body skeletal muscle mass in a subject. The invention further encompasses methods for detecting creatinine and isotope-labeled creatinine in a single sample. The methods of the invention find use, inter alia, in diagnosing disorders related to skeletal muscle mass, and in screening potential therapeutic agents to determine their effects on muscle mass.

The method of hardening, fortifying, restructuring, repairing, increasing volume or stabilizing keratinic fibers, especially human hair, includes providing a keratinic fiber treatment composition containing from 0.05 to 1.0 percent by weight of creatine, a salt of creatine, creatinine and / or a salt of creatinine, bringing the keratinic fiber treatment composition into contact with the keratinic fibers and allowing the keratinic fiber treatment composition to remain in contact with the keratinic fibers for a certain time interval.

A stable pharmaceutical formulation is provided that comprises a biologically active protein and an excipient selected from carnitine, creatine or creatinine.