36 results about "Homocystine" patented technology

Colorimetric and fluorometric methods are disclosed for the rapid, accurate, selective, and inexpensive detection of homocysteine, or of homocysteine and cysteine, or of cysteine. The methods may be employed with materials that are readily available commercially. The novel methods are selective for homocysteine, for cysteine, or for total homocysteine and cysteine, and do not cross-react substantially with chemically-related species such as glutathione. The homocysteine-selective method does not have substantial cross-reactivity to the very closely related species cysteine. The cysteine-selective method does not have substantial cross-reactivity to the very closely related species homocysteine. The methods may be used, for example, in a direct assay of human blood plasma for homocysteine levels.

The present invention provides a method of PEGylating a human truncated cystathionine β-synthase protein containing a mutation of a cysteine to a serine at amino acid position 15 (htCBS C15S). The htCBS C15S was PEGylated with one of 5 kDa, 10 kDa, or 20 kDa NHS ester PEG molecules. In-process monitoring of the PEGylation process was used in the method to reduce levels of unPEGylated htCBS C15S and htCBS C15S with insufficient PEGylation. Administration of the PEGylated htCBS C15S had efficacy throughout the course of treatment for homocystinuria.

The present disclosure provides a formulation of a drug comprising a PEGylated CBS protein having the amino acid sequence of SEQ ID NO: 1. Doses and dosing regimens for treating homocystinuria in a subject in need thereof are provided. In addition, doses and dosing regimens for reducing homocysteine (Hcy) levels or increasing cysteine (Cys) and / or cystathionine (Cth) levels in a subject in need thereof are also provided.

Disclosed is the synthesis of N-methyl-homocystines and the use thereof.

The invention discloses a synthesis method of DL-homocysteine thiolactone hydrochloride. A DL-homocystine is prepared into a catholyte with a hydrochloric acid solution as a medium, and electrolysis is conducted in an electrolysis tank to obtain the DL-homocysteine thiolactone hydrochloride, wherein according to a cathode electrode, a roughened glass carbon electrode serves as a base material, andthe surface of the base material is covered with lead-bismuth alloy. The method can be used for improving the yield of the DL-homocysteine thiolactone hydrochloride.

The invention discloses a method for synthesizing selenomethionine by adopting a one-pot method. The method comprises the following steps: mixing selenium and a reducing agent and performing a reducing reaction to obtain metal dioxide M-2Se2; adding alpha-amino protected homoserine lactones in a solution containing metal dioxide M-2Se2 and generated when the reducing reaction is finished to perform a reaction to obtain alpha-amino protected homocysteine diselenide; and reducing the alpha-amino protected homocysteine diselenide, and then performing methylation to obtain alpha-amino protected selenomethionine; and performing deprotection on the alpha-amino protected selenomethionine to obtain selenomethionine. Through the adoption of the way, the method is low in cost, high in yield and highin product purity, can be used for large batch production, avoids toxicity pollution possibly generated in the process link, and greatly reduces environmental pollution and injury to operation personnel.

The present invention discloses a DL-homocysteic acid preparation method comprising the following steps: (1) dissolving DL-homocystine in an appropriate amount of hydrochloric acid, firstly heating to the temperature of 50 DEG C-70 DEG C, then adding dropwise sodium hypochlorite, after the addition is complete, then secondly heating to the temperature of 75 DEG C-85 DEG C for reaction for 1 to 3 hours to obtain a reaction solution; and (2) concentrating the reaction solution obtained in step (1) to a small volume, then adding an appropriate amount ethanol, crystallizing, then filtering to obtain a solid crystal, and drying thee solid crystal to obtain a DL-homocysteic acid desired product. The new method for preparation of the DL-homocysteic acid by reaction of the DL-homocystine and the sodium hypochlorite in the hydrochloric acid avoids use of bromine as a reactant, and avoids pollution generated by the use of bromine, and the DL-homocysteic acid product prepared by the method is high in quality and has the purity more than 98%.