36 results about "Homocystine" patented technology

A composition comprising an amphoteric or pseudo-amphoteric agent and a polyhydroxy alpha hydroxyacid existing as a free acid, lactone, or salt, and isomeric or non-isomeric forms thereof is provided. The amphoteric or pseudo-amphoteric agent can be selected from amino acids, dipeptides, aminoaldonic acid, aminouronic acid, lauryl aminoproplyglycine, aminoaldaric acid, neuraminic acid desulfated heparin, deacetylated hyaluronic acid, hyalobiuronic acid, chondrosine, deacetylated chondroitin, creatine, creatinine, hydroxyproline, homocysteine, homocystine, homoserine, ornithine, citrulline, phosphatidylserine, and sphingomyelin. The composition may contain other additives, including cosmetic or pharmaceutical agents for topical treatment of dermatological disorders.

The present invention discloses a novel fluorescent probe capable of specifically recognizing cysteine, wherein the molecular structure formula is defined in the specification. According to the present invention, the fluorescent probe can distinguish the mercapto-containing cysteine from the mercapto-free amino acid region, can distinguish the cysteine from the homocystine and the glutathione having the similar structure, emits red light during cysteine detection, can reduce the background interference and the damage of light on tissue cells in biological applications, exhibits great Stokes shift, can reduce self-absorption so as to improve the sensitivity, can be used for the fluorescence sensing analysis of the cysteine in environments or biological samples, and has advantages of good selectivity to cysteine, high sensitivity, strong interference resistance, and good application prospect.

The invention relates to a high sensitive blood plasma homocysteine testing kit that includes sample tube with stabilizer, reference substance and quality control serum of different Hcy thickness, DL- homocystine-D8 internal standard solution, reducer and trichloroacetic acid albumen precipitation. The invention has strong specificity for sample testing, and high sensitivity. It could be accurately and rapidly used in Hcy research.

The invention relates to an environmentally-friendly method for synthesizing selenomethionine. The method comprises the following steps of: reducing alpha-amido-protected aspartate beta-alkyl grease into alpha-amido-protected homoserine, then carrying out acid catalysis for ring closing to obtain inner grease of the homoserine, then reacting with metal selenide to generate homocystine selenide, obtaining the alpha-amido-protected homoserine, and finally eliminating protection and obtaining a selenomethionine product. The environmentally-friendly method has the advantages that the highly-toxic and rotten dimethyl diselenide is prevented from being used, so that the damage to the environment and operators are greatly reduced; the yield is high, the production cost is lower, the product purity is high, and optical and pure selenomethionine can be produced by large batch; and the obtained product can be used for preparing the intermediate of a selenium-containing medicine, and solves the difficulty that the selenomethionine and the related medicines can not be popularized.

The present invention relates to a production method for DL-homocysteine lactone hydrochloride. The present invention adopts DL-methionine as the raw material, obtains DL-homocystine through the catalyzing and the hysrolyzing of concentrated sulfuric acid at a certain temperature and time, and electrolyzes the crystal of the obtained substance in chlorhydric acid medium to get the product. The present invention can realize that the content of the product is up to 99.8 percent-101.5 percent, the purity is over 99.5 percent, the yield is about 30 percent, and no water is discharged during the production process.

A method for preparing homocystine is disclosed. The method capable of eliminating a great amount of pollutants, recycling useful materials, and reducing preparation cost comprises following steps that: methionine together with a 5 to 18mol / L sulfuric acid and a 0 to 50 percent (by weight) halogen acid is heated to and kept at a temperature of 100 to 150 DEG C for 0.5 to 20 hours, with the molar ratio of methionine to sulphuric acid to halogen acid being 1 to 2-6.5 to 0-4; after the reaction liquid is cooled down, the sulfuric acid in the reaction liquid is reclaimed through dialysis until the isoelectric point of homocystine is reached, and then the precipitate is filtrated, washed, and dried to obtain homocystine. The invention is suitable for the preparation of homocystine.

The invention discloses a special high-yield and high-quality cultivation material formula and industrial cultivation method for pleurotus geesteranus. The cultivation material formula is characterized in the a cultivation material is prepared from raw materials in parts by weight as follows: 29.5%-39.5% of mushroom dreg, 29.5%-39.5% of cottonseed hulls, 10 parts of wheat husk, 1 part of calcium carbonate and 10-30 parts of culturing packing. The industrial cultivation method comprises technological steps as follows: selection of a cultivation field; preparation of the raw materials; treatment of the raw materials; culture in a mushroom house as well as fruiting and harvesting. The development demands of efficient cultivation can be met, the yield of pleurotus geesteranus is increased by 19.54%-21.39%, the content of glutamic acid is increased by 12.04%-14.29%, the content of cysteine is increased by 20%-60%, the content of threonine is increased by 7.2%-9.6%, resource utilization of the culturing packing is realized, the raw materials are easy to obtain, the cost is low, no cow dung is added, the use amount of rice straw is small, harmless, high-quality and high-yield cultivation can be realized, and the problem of shortage of the raw materials is conveniently solved.

The invention discloses a continuous production method of DL-homocysteine thiolactone hydrochloride. The method comprises the following steps: (1) continuously introducing DL-methionine as a raw material and 15-18 mol / L sulfuric acid into a liquid-liquid phase micro-channel reactor, carrying out a reaction to generate DL-homocystine, continuously introducing the DL-homocystine-containing reactionliquid flowing out of the liquid-liquid phase micro-channel reactor and hydrochloric acid into the cathode chamber of a plate-and-frame electrolytic cell, carrying out a reduction reaction on the DL-homocysteine in a hydrochloric acid system at a cathode by using graphite electrodes as the cathode and an anode to generate DL-homocysteine hydrochloride, and continuously flowing the DL-homocysteinehydrochloride-containing catholyte back to a the liquid-liquid phase micro-channel reactor, carrying out a reaction, continuously and circularly reacting until the reaction is complete, and collectingthe catholyte after the reaction is completed; and (2) carrying out impurity removal treatment on the catholyte collected in the step (1), and carrying out dehydration condensation to obtain the DL-homocysteine thiolactone hydrochloride. According to the method, the product yield is increased, the product purity is high, and the domestic medicine standard is met.

Colorimetric and fluorometric methods are disclosed for the rapid, accurate, selective, and inexpensive detection of homocysteine, or of homocysteine and cysteine, or of cysteine. The methods may be employed with materials that are readily available commercially. The novel methods are selective for homocysteine, for cysteine, or for total homocysteine and cysteine, and do not cross-react substantially with chemically-related species such as glutathione. The homocysteine-selective method does not have substantial cross-reactivity to the very closely related species cysteine. The cysteine-selective method does not have substantial cross-reactivity to the very closely related species homocysteine. The methods may be used, for example, in a direct assay of human blood plasma for homocysteine levels.

The present invention provides a method of PEGylating a human truncated cystathionine β-synthase protein containing a mutation of a cysteine to a serine at amino acid position 15 (htCBS C15S). The htCBS C15S was PEGylated with one of 5 kDa, 10 kDa, or 20 kDa NHS ester PEG molecules. In-process monitoring of the PEGylation process was used in the method to reduce levels of unPEGylated htCBS C15S and htCBS C15S with insufficient PEGylation. Administration of the PEGylated htCBS C15S had efficacy throughout the course of treatment for homocystinuria.

The present disclosure provides a formulation of a drug comprising a PEGylated CBS protein having the amino acid sequence of SEQ ID NO: 1. Doses and dosing regimens for treating homocystinuria in a subject in need thereof are provided. In addition, doses and dosing regimens for reducing homocysteine (Hcy) levels or increasing cysteine (Cys) and / or cystathionine (Cth) levels in a subject in need thereof are also provided.

Disclosed is the synthesis of N-methyl-homocystines and the use thereof.

Provided are compositions and methods for enzyme replacement therapy using modified human cystathionine beta synthase (CBS) in the treatment of homocystinuria and related diseases and disorders.

The invention discloses a synthesis method of DL-homocysteine thiolactone hydrochloride. A DL-homocystine is prepared into a catholyte with a hydrochloric acid solution as a medium, and electrolysis is conducted in an electrolysis tank to obtain the DL-homocysteine thiolactone hydrochloride, wherein according to a cathode electrode, a roughened glass carbon electrode serves as a base material, andthe surface of the base material is covered with lead-bismuth alloy. The method can be used for improving the yield of the DL-homocysteine thiolactone hydrochloride.

The invention discloses a method for synthesizing selenomethionine by adopting a one-pot method. The method comprises the following steps: mixing selenium and a reducing agent and performing a reducing reaction to obtain metal dioxide M-2Se2; adding alpha-amino protected homoserine lactones in a solution containing metal dioxide M-2Se2 and generated when the reducing reaction is finished to perform a reaction to obtain alpha-amino protected homocysteine diselenide; and reducing the alpha-amino protected homocysteine diselenide, and then performing methylation to obtain alpha-amino protected selenomethionine; and performing deprotection on the alpha-amino protected selenomethionine to obtain selenomethionine. Through the adoption of the way, the method is low in cost, high in yield and highin product purity, can be used for large batch production, avoids toxicity pollution possibly generated in the process link, and greatly reduces environmental pollution and injury to operation personnel.

The invention discloses a reactive-type fluorescence probe for distinguishing sulfydryl compounds, wherein the structure of the fluorescence probe is represented as the formula (I). R1, R2, R3 and R4 are independently selected from one of hydrogen, a C1-C8 alkyl group and a C3-C8 cycloalkyl group, and X is independently selected from halogens; or the R1 and the R4 and / or the R2 and the R3 can form a ring. The invention also discloses a synthesis method and an application of the fluorescence probe. The fluorescence probe is low in molecular weight, is high in fluorescent quantum yield, has good cytocompatibility, can generate different fluorescence enhancement signals when being reacted respectively with cysteine and homocystine, can distinguish and recognize the cysteine and homocystine well, has good stability and is easy to prepare.

The present invention discloses a DL-homocysteic acid preparation method comprising the following steps: (1) dissolving DL-homocystine in an appropriate amount of hydrochloric acid, firstly heating to the temperature of 50 DEG C-70 DEG C, then adding dropwise sodium hypochlorite, after the addition is complete, then secondly heating to the temperature of 75 DEG C-85 DEG C for reaction for 1 to 3 hours to obtain a reaction solution; and (2) concentrating the reaction solution obtained in step (1) to a small volume, then adding an appropriate amount ethanol, crystallizing, then filtering to obtain a solid crystal, and drying thee solid crystal to obtain a DL-homocysteic acid desired product. The new method for preparation of the DL-homocysteic acid by reaction of the DL-homocystine and the sodium hypochlorite in the hydrochloric acid avoids use of bromine as a reactant, and avoids pollution generated by the use of bromine, and the DL-homocysteic acid product prepared by the method is high in quality and has the purity more than 98%.

The invention discloses a tail gas treatment method for preparing DL-homocystine, and belongs to the field of medicines. The tail gas comprises sulfur dioxide gas and dimethyl disulfide gas, and oxidation absorption liquid is adopted to treat the tail gas. The method comprises the steps: treating tail gas through modified magnesium lithium silicate and then introducing the tail gas into oxidation absorption liquid to be treated, layering a solution obtained after treatment stands still, and taking an oil phase after water-oil separation; and finally, extracting, condensing and removing the oil phase by adopting an extracting agent. According to the tail gas treatment method for preparing the DL-homocystine, dimethyl disulfide can generate colorless and odorless dimethyl sulfoxide and sulfur dioxide can generate sulfuric acid respectively by adopting the oxidation absorption liquid, so emission of toxic and odorous gases is eliminated fundamentally; meanwhile, even if the oxidation absorption liquid enters a Fenton oxidation unit of a sewage treatment system for operation, toxic gas cannot overflow again due to re-acidification.

The present invention discloses a novel fluorescent probe capable of specifically recognizing cysteine, wherein the molecular structure formula is defined in the specification. According to the present invention, the fluorescent probe can distinguish the mercapto-containing cysteine from the mercapto-free amino acid region, can distinguish the cysteine from the homocystine and the glutathione having the similar structure, emits red light during cysteine detection, can reduce the background interference and the damage of light on tissue cells in biological applications, exhibits great Stokes shift, can reduce self-absorption so as to improve the sensitivity, can be used for the fluorescence sensing analysis of the cysteine in environments or biological samples, and has advantages of good selectivity to cysteine, high sensitivity, strong interference resistance, and good application prospect.