135results about "Carbon-sulfur lyases" patented technology

The present invention relates generally to production methods, enzymes and recombinant yeast strains for the biosynthesis of clinically important prenylated polyketides of the cannabinoid family. Using readily available starting materials, heterologous enzymes are used to direct cannabinoid biosynthesis in yeast.

The invention discloses application of genetically engineered bacterium VNP-20009-M of attenuated salmonella typhimurium in preparation of medicines for preventing and treating metastasized cancers. The genetically engineered bacterium VNP20009-M has a tumor targeting property on cancer cells, has a remarkable effect for inhibiting metastasis and growth of cancer cells, and can be used for preparing medicines for preventing and treating metastasized tumors.

Methods and composition related to the engineering of a novel protein with methionine-γ-lyase enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-γ-lyase (CGL) comprising one or more amino acid substitutions and capable of degrading methionine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with methionine depletion using the disclosed proteins or nucleic acids.

A substantially purified substance having the properties of a bacterial microcin methionine analog, methionine synthesis inhibitor, tRNA-methionine synthase inhibitors or methionine competitive inhibitor capable of inhibiting tumor cell growth without inhibiting the growth of normal cells or treating neoplastic diseases, and may be used alone or in combination with other anti-cancer agents. The purified substance may also have anti-hyperhomocysteineuria and / or anti-infective properties, such as antifungal activity. The purified substance can be safely administered to animals including humans for the treatment of neoplastic, hyperhomocysteinemia and / or infectious diseases for the treatment of those diseases.

Methods and compositions relating to the engineering of an improved protein with methionine-γ-lyase enzyme activity are described. For example, in certain aspects there may be disclosed a modified cystathionine-γ-lyase (CGL) comprising one or more amino acid substitutions and capable of degrading methionine. Furthermore, certain aspects of the invention provide compositions and methods for the treatment of cancer with methionine depletion using the disclosed proteins or nucleic acids.

The present disclosure relates to the production of cannabinoids in yeast. In one aspect there is provided a genetically modified yeast comprising: one or more GPP producing genes and optionally, oneor more GPP pathway genes; two or more olivetolic acid producing genes; one or more cannabinoid precursor or cannabinoid producing genes; one or more Hexanoyl-CoA producing genes, and at least 5% dryweight of fatty acids or fats.

The invention discloses a preparation method and low-temperature continuous stirring ultrafiltration device for an anti-tumor and anti-infection allicin injection. The preparation method for the anti-tumor and anti-infection allicin comprises the following two steps: 1. extracting allicin; 2. in a clean environment, diluting the allicin extracted in the step 1 by utilizing a dissolvent precooled to 0-4 DEG C to obtain an allicin solution; and packaging the allicin into allicin injections of different specifications according to ampoule required to fill nitrogen or argon. The anti-tumor and anti-infection allicin injection can be used for intravenous drip to treat diseases such as sepsis or tumor and the like. The invention simultaneously discloses a low-temperature continuous stirring ultrafiltration device for preparing the allicin injection. The invention has the advantages that the formula is reasonable, and the preparation technology is simple; the defects of the prior art are overcome; and the product purity is high, and the performance is stable.

The invention belongs to the field of biotechnology, and provides an avian pathogenic escherichia coli strain, which is an avian pathogenic escherichia coli luxS and aroA double-gene-deleted strain with the preservation numbr of CGMCC10601. The avian pathogenic escherichia coli strain provided by the invention can be used for preparing avian pathogenic escherichia coli inactivation and attenuated vaccine and bacterial ghost vaccine, and preventing avian pathogenic escherichia coli infection.

The invention discloses a methionine lyase, as well as an encoding gene and a biosynthesis method thereof. A gene for encoding the methionine lyase of which the sequence is as shown in SEQ ID No.1 is separated from tuber melanosporum. The invention further provides an efficient biosynthesis method for the methionine lyase. The efficient biosynthesis method comprises the following steps: (1) cloning the gene, of which the sequence is as shown in SEQ ID.1, for encoding the methionine lyase in a yeast expression vector to construct a recombinant yeast expression vector; (2) converting the recombinant yeast expression vector in brewer's yeast to obtain an expression strain; (3) inducing the expression strain to express the methionine lyase, collecting a thallus after induced expression, and purifying the expressed recombinant methionine lyase. The purity of the recombinant methionine lyase prepared according to the biosynthesis method is 90% or higher, and the methionine degrading efficiency can reach 0.53 + / - 0.0030 [mu]M MTL.h<-1>.mg protein<-1>.

The present invention provides an olivetol synthase variant and an engineered microorganism expressing it for the production of olivetol and olivetolic acid, more specifically an engineered Escherichia coli, wherein the engineered Escherichia coli is modified to express olive The alcohol synthase variant is characterized in that the olive alcohol synthase variant comprises one or more mutations selected from the following mutations compared with its wild type: I303T, I52L, S56A, H262M and K263R. The engineered E. coli achieves improved yields of olivetol and olivetolic acid.

The invention discloses a method for preparing allinase, allicin and garlic polysaccharide from garlic slice processing wastewater. The method includes steps: (1) after pre-cooling of the garlic slice processing wastewater, adopting an ultra-filtration membrane being 10,000Da in molecular weight cut-off to realize ultra-filtration condensation, and combining cut-off liquid to obtain allinase crude fluid; (2) subjecting ultra-filtration filtrate to nano-filtration condensation through a 300Da nano-filtration membrane, extracting nano-filtration concentrate with CH2Cl2 for multiple times, keeping raffinate for preparation of garlic polysaccharide at the next step, combining CH2Cl2 extraction liquid, and removing CH2Cl2 by reduced pressure distillation to obtain oily liquid mainly composed of allicin; (3) collecting CH2Cl2 raffinate obtained at the step (2), and performing spray drying after reduced pressure distillation to obtain garlic polysaccharide extract. According to the method, respective extraction of various biological active components in the garlic slice processing wastewater is realized, three valuable products are acquired, recycling of the garlic slice processing wastewater is realized, and problems of resource waste and environment pollution in a garlic slice processing process are solved.

The invention belongs to the technical field of formulas of medicament for preventing and treating physical diseases and particularly relates to composition capable of preventing and treating respiratory system diseases like novel coronavirus infection and other diseases. Alliin, alliinase and type II transmembrane serine protease inhibitor are taken as basic effective ingredients, the compositionis administered in a way of inhalation by the lung, can kill physical diseases caused by bacteria such as methicillin-resistant staphylococcus aureus, multidrug resistance type escherichia coli, multidrug resistance type pseudomonas aeruginosa, multidrug resistance type mycobacterium tuberculosis and the like, viruses such as SARS-CoV-2 and the like, fungi such as multidrug resistance type candida albicans and the like, parasites and the like in a respiratory system and also can prevent fat accumulation in blood vessels of a human body, reduce the blood pressure, the cholesterol level in blood and destroy cancer cells of multiple epithelial tumors of breast cancer, ovarian cancer, colon cancer, renal carcinoma, liver cancer, lung cancer, mesothelioma and prostatic cancer, and the composition has no any toxic or harmful functions and risks and has broad application prospects.

The present invention relates generally to production methods, enzymes and recombinant yeast strains for the biosynthesis of clinically important prenylated polyketides of the cannabinoid family. Using readily available starting materials, heterologous enzymes are used to direct cannabinoid biosynthesis in yeast.