50results about How to "Has antitumor effect" patented technology

The invention discloses application of genetically engineered bacterium VNP-20009-M of attenuated salmonella typhimurium in preparation of medicines for preventing and treating metastasized cancers. The genetically engineered bacterium VNP20009-M has a tumor targeting property on cancer cells, has a remarkable effect for inhibiting metastasis and growth of cancer cells, and can be used for preparing medicines for preventing and treating metastasized tumors.

The invention relates to a method for improving the quality of animal semen by using chestnut flower water extract. After weaning male mammals, the chestnut flower water extract is supplemented by gavage or added to feed every day, and the intake dose of small male mammals is 0.01-20mg / kg body weight, the ingestion dose of medium-sized male mammals is 0.1-20mg / kg feed, the chestnut flower water extract is a freeze-dried powder obtained by ultra-microfiltration after ultrasonic extraction. The present invention proposes for the first time that the water extract of chestnut flower is extracted by ultrasonic waves, and after further ultrafine filtration, supplementary feeding to male mammals can significantly improve the semen quality of animals, and the timing of supplementary feeding is immediately after weaning of male mammals. The scheme enables male mammals to better establish a reproductive system, fully taps the genetic potential of male animals, and expands the range of utilization of chestnut flower water extract.

The invention belongs to the technical field of medicines, and in particular relates to a camptothecin glycoconjugate and its preparation method and application. The invention provides a camptothecin glycoconjugate, and the camptothecin glycoconjugate is synthesized by linking camptothecin and hexose with adipic acid as a linker. Experimental results show that the camptothecin glycoconjugate has an anti-tumor effect, and has high efficiency and low toxicity compared with camptothecin. GLUT1 is widely distributed on the blood-brain barrier and red blood cells. The experimental results show that the camptothecin glycoconjugates of the present invention can be recognized and transported by GLUT1, and enter into tumor cells through the GLUT1 pathway to achieve anti-tumor effects. Moreover, the camptothecin glycoconjugates of the present invention Glycoconjugates, as a cytotoxic quinoline alkaloid, can inhibit DNA topoisomerase (TOPO I).

The invention discloses a fusion protein of PA4-Fc and a coding gene and application thereof. The protein provided by the invention is any one of 1) a protein formed by amino acid residue sequences of a sequence 2 in a sequence table and 2) a protein which is derived from 1), is obtained by subjecting the amino acid residue sequences of the sequence 2 in the sequence table to substitution and / or deletion and / or addition of one or more amino acid residues and has the same functions. Experiments prove that a DNA sequence expressing the fusion protein PA4-Fc is constructed by optimizing codons and is imported into cells via carriers to express and purify the fusion protein PA4-Fc; the fusion protein can suppress growth of tumor cells and has an antitumor effect; besides, as the fusion protein contains a human IgG1Fc fragment, the half-life period of the fusion protein in bodies can be lengthened.

The invention relates to application of an ophicalcitum extract and a method for preparing the same, and discloses a method for preparing ophicalcitum (with serpentine serving as a main component) extracting solution and anticancer application thereof. The extract can be extracted from ophicalcitum, and serpentine simple ore, serpentine-containing marble and rock having the characteristic of a serpentine ore family. The extract is originated from an ophicalcitum medicinal material, medicinal material submicron powder, mineral suspended particles and ophicalcitum natural nano-fiber thereof (the origins comprise raw / forged products). Researches on in-vivo and in-vitro pharmacological activities indicate that the ophicalcitum extract has very high anticancer activity and is expected to be developed into a new anticancer medicament using the ophicalcitum extract as a raw material and having wide clinical use value and commercial development value.

The invention belongs to the field of the traditional Chinese medicine and discloses a gynostemma pentaphylla flavonoid compound, a preparation and an application thereof in an antitumor drug. The method comprises the following steps: (1) extracting the rough gynostemma pentaphylla powder by adopting an alcohol solution for heating and refluxing, centrifuging and concentrating, thereby acquiring gynostemma pentaphylla ethyl alcohol extract; (2) using water for dissolving the extract, filtering, adopting petroleum ether for extracting the filtrate and adopting ethyl acetate for extracting lower-layer water phase; (3) performing vacuum concentration on the ethyl acetate phase, drying and adopting a chloroform-methyl alcohol mixed solvent for isocratic elution in a silica gel column, therebyacquiring multiple fractions; (4) pouring the fractions onto a gel column, eluting with methyl alcohol and collecting the fractions with equal analogous values; (5) pouring the fractions onto a ODS column, using methyl alcohol-water for isocratic elution, collecting the fractions and drying, thereby acquiring the gynostemma pentaphylla flavonoid compound. The gynostemma pentaphylla flavonoid compound prepared according to the invention has the advantages of high anti-tumor activity, low dosage and no toxic or side effect to normal human hepatic cells in same dosage.