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A method for improving the quality of animal semen by using chestnut flower water extract

A technology of water extract and chestnut flower is applied in the field of improving animal semen quality to achieve the effects of improving semen quality, promoting the development of reproductive system and expanding the scope of utilization

Active Publication Date: 2021-12-28
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, studies have found that there are many active ingredients in chestnut flowers, which have anti-oxidation, anti-aging, anti-inflammatory and other effects. However, it is unknown whether the active ingredients in chestnut flowers also have the effect of improving semen quality, and there is no literature on the use of chestnut flowers. Method of water extract to improve semen quality

Method used

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  • A method for improving the quality of animal semen by using chestnut flower water extract
  • A method for improving the quality of animal semen by using chestnut flower water extract
  • A method for improving the quality of animal semen by using chestnut flower water extract

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Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1: preparation Chinese chestnut flower water extract

[0029] 1) Pick fresh chestnut male flowers in full flowering stage and immediately place them in ultrapure water with a solid-to-liquid ratio of 35g / L. Use a homogenizer to fully homogenize chestnut flowers, and then ultrasonically extract them in an ultrasonic instrument with a power of 780W at 45°C for 1 hour. Subsequently, it was centrifuged at 12000r / min for 50min, the aqueous layer solution was collected, and the obtained aqueous layer solution was freeze-dried to obtain a light yellow powder;

[0030] 2) The light yellow powder obtained in step 1) is dissolved in ultrapure water again, and the ultra-microfiltration membrane with a pore size of 0.25 μm is used for suction filtration, and the aqueous solution obtained by suction filtration is freeze-dried again to obtain a solid powder of chestnut flower water extract ;

[0031] 3) The solid powder of chestnut flower water extract obtained in step 2)...

Embodiment 2

[0032] Embodiment 2: Mouse feeding test

[0033] 240 3-week-old mice (with uniform body weight) of the healthy ICR strain were randomly assigned to 8 groups (30 / group), and the mice were eating and drinking normally, and were raised in a unified SPF rat room . Take a certain amount of chestnut flower water extract powder obtained in step 3) of Example 1 every day according to the design in Table 1, dissolve it in purified water, and prepare the required chestnut flower water extract solution. Each mouse was fed with the water extract solution of chestnut flower matching its body weight by intragastric administration on time every day. Each mouse was gavaged with 0.1 mL water extract solution of chestnut flower. After 5 weeks of gavage, the semen of the mice was collected, and the sperm motility and density were analyzed with a semen quality analyzer. The test results are shown in Table 1:

[0034] Table 1 Effect of water extract of Chinese chestnut flower supplementation o...

Embodiment 3

[0037] Embodiment 3: Pig feeding test

[0038] In the cooperative experimental base of our school, 70 healthy weaned (35-day-old) boars were selected and randomly divided into 7 groups (10 heads / group). All pigs are in a large breeding room, eating and drinking normally, and are under unified management. Take a certain amount of chestnut flower water extract powder obtained in step 3) of embodiment 1 according to the design in Table 2 every day, and fully mix the weighed animal concentrate and chestnut flower water extract powder. Feed the prepared feed concentrate containing chestnut flower water extract to pigs on time every day. Feed until 6 months old, collect the semen of the pigs, and use the semen quality analyzer to analyze the sperm motility and density.

[0039] Table 2 Effects of pig supplementation with chestnut flower water extract on semen quality

[0040] Daily Supplement Dose of Chestnut Flower Water Extract Sperm concentration (×10^9) Sperm mo...

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Abstract

The invention relates to a method for improving the quality of animal semen by using chestnut flower water extract. After weaning male mammals, the chestnut flower water extract is supplemented by gavage or added to feed every day, and the intake dose of small male mammals is 0.01-20mg / kg body weight, the ingestion dose of medium-sized male mammals is 0.1-20mg / kg feed, the chestnut flower water extract is a freeze-dried powder obtained by ultra-microfiltration after ultrasonic extraction. The present invention proposes for the first time that the water extract of chestnut flower is extracted by ultrasonic waves, and after further ultrafine filtration, supplementary feeding to male mammals can significantly improve the semen quality of animals, and the timing of supplementary feeding is immediately after weaning of male mammals. The scheme enables male mammals to better establish a reproductive system, fully taps the genetic potential of male animals, and expands the range of utilization of chestnut flower water extract.

Description

technical field [0001] The invention relates to the field of animal reproductive physiology and reproduction technology, in particular to a method for improving the quality of animal semen by using chestnut flower water extract. Background technique [0002] In recent years, due to the excessive or incorrect application of pesticides and fertilizers, environmental pollution, climate change and other reasons, the sperm motility of male animals has decreased, the concentration of sperm has decreased, and the rate of sperm deformity has increased, resulting in a substantial increase in the rate of male infertility. This is a worldwide problem that has attracted widespread attention from all over the world. However, there is no good method to improve semen quality to treat male infertility. Therefore, scientists are looking for effective drugs or methods to improve the semen quality of male animals. [0003] Chestnut (Castanea mollissima BL.), also known as: chestnut, chestnut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/30
CPCA23K10/30
Inventor 赵勇沈伟孙中义李兰于帅
Owner QINGDAO AGRI UNIV
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