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Artificial spawning induction and insemination method for nibea albiflora

A technology for artificial induction of labor and yellow croaker, applied in the biological field, can solve the problems of inability to meet the directional mating of fish genetics and breeding, inability to realize spawning time and spawning quantity, spawning asynchrony, etc., and avoid spawning and ovulation. Accurate synchronization, improve survival rate and work efficiency, and prolong the effect of threshold window time

Active Publication Date: 2019-08-27
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the artificial breeding of yellow croaker relies on the natural maturation of the parents to achieve spawning. Due to the difference in the maturity of the parents of croaker, the gonad development of male and female fish is not synchronized, and the gonads of male fish mature earlier than female fish, and the gonad development of female fish is generally slow. There are obvious individual differences, which can easily cause asynchronous spawning, and artificial control of spawning time and spawning amount cannot be realized, which is not conducive to production, nor can it meet the requirements of fish genetic breeding for directional mating

Method used

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  • Artificial spawning induction and insemination method for nibea albiflora
  • Artificial spawning induction and insemination method for nibea albiflora
  • Artificial spawning induction and insemination method for nibea albiflora

Examples

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Embodiment 1

[0033] The method for preparing a composition with stability, comprising, a, preparing a buffer solution and adjusting its pH; b, adding oxytocin having an oxytocin function to the buffer solution; and thereafter, c, adding to the dispersion obtained in step b Add at least one non-ionic cellulose ether; the buffer solution is alkaline; the oxytocin includes N-acetyl-L-cysteine-coated microspheres. The stability of the biological substance in the composition produced by the method is improved, in particular the biological activity and therapeutic effect of the presented composition is improved by avoiding excessive degradation or polymerization, or other structural changes of the oxytocin substance, The biological activity or therapeutic activity of oxytocin is improved and prolonged, and it is easier for the user to handle. Due to the improved biological activity, the composition does not always need to be stored in a cold environment (-15 ~ -5°C) It can be more conveniently s...

Embodiment 2

[0045] The artificial oxytocin fertilization method of yellow croaker, the specific measures are as follows:

[0046] 1) After mixing N-acetyl-L-cysteine ​​and gelatin, add 6 times the amount of water, let it stand for swelling for 2.5 hours, heat to 70°C and stir until dissolved to make an internal water phase, the above-mentioned internal water phase The weight proportions of N-acetyl-L-cysteine ​​and gelatin are respectively 11% and 19%, and the above-mentioned inner water phase also contains 0.15% uric acid and 0.05% gluconic acid-δ - Lactone;

[0047] 2) Dissolve ethyl cellulose in dichloromethane to make an oil phase with a weight ratio of 13%, then add the inner water phase into the oil phase at a weight ratio of 1:22, and ultrasonically Crush for 5 minutes to form colostrum;

[0048] 3) Take polyvinyl alcohol and add water and heat it to 70°C and stir until it dissolves to make an external water phase with a weight concentration of 1.5%, and then disperse the colostr...

Embodiment 3

[0056] The differences between this embodiment and embodiment 2 are:

[0057] The composition in step 6) is injected into the broodstock in the form of injection administration once a day on the 58th-60th day of cultivation, and the dosage is 50 mg / kg body weight each time;

[0058] Other steps are consistent with embodiment 2, cultivate yellow croaker fry.

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Abstract

The invention belongs to the technical field of biology and provides an artificial spawning induction and insemination method for nibea albiflora. The method includes: in a nibea albiflora parent breeding period, feeding nibea albiflora parents with a prepared stable composition, and performing nutrition enhancement; after sexual maturity of the parents, artificially collecting spawns and semen tocomplete insemination and obtain inseminated spawns; hatching the inseminated spawns, and carrying out fry breeding. Before artificial spawn and semen collection, injecting the nibea albiflora parents with spawning induction hormones is avoided. By adoption of the artificial spawning induction and insemination method, the spawns and semen can be regulated to realize synchronous maturity finally,so that nonsynchronous and unsmooth spawning and semen discharging can be avoided, and after-spawning parent fish death loss is avoided; large spawning quantity of female fish and high semen quality of male fish are realized, and the hatching rate and the effective hatching quantity can be increased so as to increase fry output; the composition is stable and easy to preserve and is capable of increasing the fat, protein and total sugar accumulation in testes and ovaries to enable synchronous development and maturity of testes and ovaries of the parents, and the spawning amount of parental female fish is increased.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an artificial induced fertilization method for yellow croaker. Background technique [0002] Yellow croaker (Nibea albiflora), commonly known as yellow croaker, belongs to Perciformes, Chrysostidae, and genus Yellow croaker. Its body shape is similar to yellow croaker. It is a fish in the middle and lower waters of the sea, mainly inhabiting sandy mud bottoms It is an important multiplication and breeding species in the southeast coast of my country. Its meat is delicious, nutritious, and has high nutritional and medicinal value. The market demand on the rise. It is worth mentioning that the yellow croaker has an insect-resistant peptide gene that the large yellow croaker does not have, and has special immunity to stimulating Cryptocaryonia, and can be used as the preferred alternative breeding species in areas with high incidence of white spot disease. [0003] Due to th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10A01K61/17
CPCA01K61/10A01K61/17Y02A40/81
Inventor 苗增良
Owner ZHEJIANG OCEAN UNIV
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