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Application of protosappanin B in preparation of bladder cancer resistant perfusion fluid

A technology of hematoxylin and bladder cancer, applied in the application field of preparing anti-bladder cancer perfusion solution

Inactive Publication Date: 2012-09-19
山西华尚汇商贸有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Domestic research reports on protohematoxylin B were only found in the use of standard products when the quality standards of traditional Chinese medicine hematoxylin were established (Chinese Journal of Traditional Chinese Medicine, 2010.35(16): 2068-2070); Japanese scholar Sasaki Y et al found that protohematoxylin B can inhibit sub The role of oleic acid oxidation (Biol Pharm Bull, 2007.30(1):193-196); Washiyama M et al. reported that prohematoxylin B and other hematoxylin extracts have good anti-inflammatory effects (Biol Pharm Bull 2009.32(5):941- 944); At present, there are no more research reports on prohematoxylin B at home and abroad

Method used

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  • Application of protosappanin B in preparation of bladder cancer resistant perfusion fluid
  • Application of protosappanin B in preparation of bladder cancer resistant perfusion fluid
  • Application of protosappanin B in preparation of bladder cancer resistant perfusion fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Embodiment 1: The killing effect experiment of protohematoxylin B on bladder cancer cells is illustrated below in the pharmaceutical field:

[0011] 1. Experimental cells and drugs:

[0012] Cell lines: human bladder cancer cell line T24, purchased from Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences; mouse bladder cancer cell line BTT, gifted by Yang Xiaofeng, director of the Urology Department of the First Affiliated Hospital of Shanxi Medical University;

[0013] Protohematoxylin B: Prepare a liquid medicine with a concentration of 0.4% with water for injection, and sterilize it under high pressure;

[0014] Trypan blue staining solution.

[0015] 2. Experimental method:

[0016] (1) Digest, collect cells, and centrifuge at 1000rp / 5min. Human bladder cancer cell line T24 and mouse bladder cancer cell line BTT are respectively adjusted to 2× with water for injection. / ml.

[0017] (2) In the prohematoxylin B group, 500 μL of T24 cell susp...

Embodiment 2

[0022] Example 2: The tumor inhibitory effects of mitomycin and different doses of prohematoxylin B on T739 mice intraperitoneally inoculated with BTT cells were observed through the survival time of mice.

[0023] 1. Experimental materials

[0024] Experimental animals: 50 T739 mice, female, weighing 24-36 g; provided by the Animal Laboratory of Shanxi Cancer Institute, the production license number is SCXK (Jin) 2007-0001.

[0025] Experimental cells: mouse bladder cancer cell line BTT, donated by Prof. Yang Xiaofeng from Department of Urology, Shanxi Medical University.

[0026] Experimental drugs: mitomycin (commonly used anti-cancer western medicine), Zhejiang Hisun Pharmaceutical Co., Ltd., batch number 100401; prohematoxylin B: batch number: 20101010-2.

[0027] 2. Experimental method:

[0028] (1) Inoculation: Grind the BTT solid tumor under sterile conditions, make a cell suspension with normal saline, and adjust the concentration to 5×10 6 / ml, intraperitoneal ino...

Embodiment 3

[0040] 1. Experimental materials:

[0041] Experimental cells: Human bladder cancer T24 cell line was quoted from Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. The T24 cell line was cultured in RPMI-1640 medium containing 10% fetal bovine serum. The cells in the logarithmic growth phase were selected for the experiment. They were digested with trypsin containing EDTA, and the cell viability was measured by the trypan blue exclusion method, and fused with PBS buffer. (phosphate buffered saline) to make a concentration of 1x / ml of cell suspension.

[0042] Experimental drug: prohematoxylin B, batch number: 20101010-2; mitomycin, a commonly used anticancer drug, produced by Zhejiang Hisun Pharmaceutical Co., Ltd., specification 2mg / bottle, production batch number: 110716.

[0043] Experimental animals: 60 female BALB / C nude mice, purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., SPF grade, 7-9 weeks old, weighing 20g-22...

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Abstract

The invention relates to application of protosappanin B, in particular to application of a 0.2 to 2.4 percent protosappanin B solution in preparation of bladder cancer resistant perfusion fluid. For filling up the research blank of the protosappanin B at home and abroad, the invention provides new application of the protosappanin B. Human bladder cancer cell strains T24 or mouse bladder cancer cell strains BTT are inoculated to mouse bodies to form bladder cancer, and the mice are grouped and respectively subjected to experiments with injection water, mitomycin and the protosappanin B; experiment results show that the lives of the mice injected with the mitomycin and the protosappanin B are prolonged, the tumor weight is reduced, and a tumor inhibiting effect is achieved; and the protosappanin B has a remarkable bladder cancer inhibiting effect, and can be widely applied to preparation of the bladder cancer resistant perfusion fluid.

Description

technical field [0001] The invention relates to the use of protohematoxylin B, in particular to the application of a protohematoxylin B solution with a concentration of 0.2-2.4% as an anti-bladder cancer perfusion solution. Background technique [0002] Sumac (Caesalpinia sappan Linn.), also known as Sufang, Fangfangmu and Sufang, is a small tree with a flowering period from May to October and a fruiting period from July to March of the following year. my country's Yunnan, Guizhou, Sichuan, Guangxi, Guangdong, Fujian and Taiwan provinces have cultivated sumac, which is native to India, Myanmar, Vietnam, the Malay Peninsula and Sri Lanka. At present, a variety of chemical components have been isolated from hematoxylin, which can be divided into five parts according to their skeleton types: hematoxylins, prohematoxylins, homoisoflavones, hematoxychalcones, and phenylpropanoids. Protohematoxylins include prohematoxylin A, protohematoxylin B, 10-O-methylprotohematoxylin B, hete...

Claims

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Application Information

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IPC IPC(8): A61K31/335A61P35/00A61P13/10
Inventor 任连生王国平王晋芬杨喜花
Owner 山西华尚汇商贸有限公司
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