2678results about How to "Good killing effect" patented technology

The invention relates to a novel spirodiclofen compound and a preparation method and application thereof. The structure of the novel spirodiclofen compound is shown in a general formula I. The synthesis method of the novel spirodiclofen compound comprises the steps of: in the presence of an acid binding agent, in an aprotic solvent, reacting a compound with a structure formula II with chloro-carbonic ester with a structure formula shown in the description at room temperature, and separating and purifying a product to prepare the novel spirodiclofen compound. The novel spirodiclofen compound can be used for killing pests or mites.

A food cleaning device comprises a cleaning water tank, a water pump and an electrolyzed water generator all provided with water inlets and water outlets and connected to form a closed circulation loop by pipelines. An ultrasonic transducer is provided in the water tank. Food can be cleaned safely and effectively by the inventive device with disinfection and pesticide residue removing functions. The invention is provided with advantages of time saving, labor saving, water saving, high cleaning ratio, and body health benefiting, thereby the invention is capable of being widely used by household, school, mess hall, catering industry and vegetable cleaning industry.

A nano-class mesoporous Ag-carried antibacterial agent used for detergent, cosmetics, water treating, textile, etc contains inorganic oxide as carrier with orderly mesopore structure and Ag as active antibacterial component. Its advantages are large specific surface area, broad spectrum, high effect and durability, and no damage to people.

The invention discloses a Webshell detection method based on a deep neural network, and a system thereof. A recursion loop neural network based on an abstract syntax tree automatically obtains the morphology and grammar information of a script by aiming at a script language, the hierarchical structure characteristics of the abstract syntax tree is used for finishing feature abstraction and WebShell detection, including preprocessing, sample generation and WebShell detection; firstly, the morphology and grammar information of the script is automatically obtained, and then, the recursion loop neural network based on the abstract syntax tree is used for finishing the feature abstraction and the WebShell detection. The method disclosed by the invention has the advantages of being low in deployment cost, good in portability and high in detection accuracy.

The anti-fake pesticide-germicide is prepared with boron, saponin, pyrethroid, organophosphorus pesticide and alcohol. After the pesticide-germicide is set into various kinds of dry small mouth bottle with the lid opened for 0.25-2 hr, white snowflake-like crystal will grow around the bottle mouth, and this makes it possible for the user to identify the truth of the pesticide-germicide. Compared with pesticide-germicide of alone pyrethroid and organophosphorus pesticide, the pesticide-germicide of the present invention has greatly raised effect, lowered toxicity, lowered cost and reduced consumption.

The invention discloses a controlled-release multilayer drug-loaded artificial bone and a preparation method thereof. The controlled-release multilayer drug-loaded artificial bone is a multilayer structure which is coated layer by layer and is composed of artificial bone carrier materials loaded with drugs or a multilayer structure which is coated layer by layer and is formed by alternately arranging the artificial bone carrier materials loaded with the drugs and artificial bone carrier materials loaded with no drugs; the outline of the controlled-release multilayer drug-loaded artificial bone can be cylindrical, cuboid, square or irregular; and the artificial bone carrier materials loaded with the drugs at different layers comprise the same or different drugs. The controlled-release multilayer drug-loaded artificial bone is prepared by adopting a three-dimensional stereoscopic printing rapid prototyping technology, and the drugs distributed at the different layers are released from outside to inside layer by layer, thereby being capable of realizing multidrug combined action, regulating the releasing sequence and time of the drugs and selecting appropriate drugs to load so as to achieve the individualized treatment goal. The invention can be applied to carrying out local chemotherapy and filling and repairing bone coloboma after eradication focuses of various infections, concretions, tumours and the like of the orthopedics department.

The invention provides a lipidosome-polymer drug-loaded nanoparticle and a preparation method and application thereof. The lipidosome-polymer drug-loaded nanoparticle comprises a three-layer structure, wherein the innermost layer is the drug-loaded nanoparticles formed by taking an amphiphilic cationic polymer as a carrier-coated chemotherapeutic drug; the intermediate layer is a platelet inhibitor layer adsorbed onto the surface of the drug-loaded nanoparticles; and the outermost layer is a lipid bilayer connected with tumor microenvironment responsiveness polypeptides. The lipidosome-polymer drug-loaded nanoparticle disclosed by the invention is capable of specifically targeting tumor tissues, has tumor microenvironment responsiveness, and can achieve the effects of improving tumor vascular permeability without influencing functions of blood vessels at normal tissue or cell parts, enhancing permeability and retention of the drug-loaded nanoparticles to tumor cells, enhancing the EPR effect, improving enrichment of the drug-loaded nanoparticles at tumor location and realizing high tumor killing efficiency.

The invention belongs to the technical field of epidemic prevention in livestock farms, and in particular relates to a Chinese herbal medicine environmentally-friendly sanitizer for a dairy farm. The sanitizer provided by the invention comprises the following bulk pharmaceutical chemicals in parts by weight: 25-32 parts of rheum officinale, 8-12 parts of fructus forsythia, 24-30 parts of folium artemisiae argyi, 10-15 parts of honeysuckle, 5-8 parts of fructus cnidii, 3-5 parts of rhizoma atractylodis, 3-5 parts of dandelion, 9-12 parts of radix sophorae flavescentis, 12-15 parts of Syzygium aromaticum, 18-25 parts of indigo naturalis and 15-18 parts of mint. The sanitizer provided by the invention is stable and effective in sanitization for the livestock farms, achieves the equal effect of the chemical sanitizer, has no medicament residue, is environmentally-friendly, guarantees the diary health and safety, and has obvious economical benefit and social benefit.

The invention provides sterilizing bath lotion for furry pets, which comprises surfactant, humectant, hair care agent, chelating agent, bactericide, thickening agent and essence, wherein the bactericide is one of stable liquid chlorine dioxide, stable solid chlorine dioxide or sodium chlorite. The sterilizing bath lotion of the invention overcomes the defects of the existing product or the prior art, and really realizes synchronous completion of three effects of removing dirt, caring hair and quickly sterilizing. The sterilizing bath lotion has the advantages of mild property, no any stimulation to the fur of animals and the skin of people, good dirt removing and sterilizing effects, low foam, easy cleaning, no residue, safety and environment friendliness, and meanwhile plays a role in caring the fur of the animals so that the fur of the washed pet does not knot; and the sterilizing bath lotion is glossy, flexible and easy to card, and is quite suitable for the daily care of the furry pets.

The invention provides a medicament for treating gynecological inflammation, which comprises the following components in parts by weight: 5 to 30 parts of common cnidium fruit, 4 to 30 parts of golden cypress, 4 to 30 parts of lightyellow sophora root, 5 to 30 parts of sessile stemona root, 0.1 to 2 parts of borneol, 0.1 to 2 parts of menthol, 0.1 to 2 parts of chlorhexidine acetate, and 0.1 to 2 parts of vitamin E. Compared with the prior art, the invention adopts a compound preparation consisting of classical Chinese medicaments and western medicaments, and has the characteristics of quickly killing pathogenic bacteria of western medicaments as well as diminishing inflammation and resisting bacteria of Chinese medicaments. Microbiological experiments and toxicant experiments prove that: the Chinese medicinal preparation has an excellent killing effect on pathogenic bacteria causing gynecological inflammation, such as staphylococcus aureus, candida albicans, escherichia coli and other pathogens, has the effects of treating female pruritus vulvae, has an accurate curative effect, low toxic or side effect, excellent functions of inflammation diminishing, sterilization and itching relieving.

The invention discloses an inorganic antibacterial fiber of a coating layer and a preparation method thereof. The invention leads four-needle zinc oxide whisker that is blended with silver, ferric oxide, titanium dioxide or the mixture of the silver, the ferric oxide and the titanium dioxide to be modified, and expands the responsiveness of the four-needle zinc oxide whisker in the field of visible lights. The four-needle zinc oxide whisker or the modified four-needle zinc oxide whisker are loaded onto the fiber, and due to the special space structure, the four-needle zinc oxide whisker has strong holding power to the fiber and can be well loaded onto the fiber, thus well combining the high catalytic activity of the four-needle zinc oxide whisker and the high specific surface property of the fiber, greatly improving the catalytic efficiency of optical catalysts, and simultaneously solving the recycling problem of the optical catalysts.

The invention relates to an extracting and processing technique of carrageen by utilizing eucheuma, which comprises the procedures of cleaning, alkali treatment, washing, enzymatic hydrolysis, extracting, filtering, potassic alteration, dehydration, crushing and drying. By adopting enzyme biotechnology technology, the extraction rate and quality of carrageenan are improved, while the production cycle and energy consumption thereof being lowered. Colloid dissolves out of carrageen cells completely after the organization of cellulose in carrageen cell wall being destroyed during the procedure of enzymatic hydrolysis, thus improving the extraction rate of carrageenan. Meanwhile, the time of boiling procedure is shortened and the temperature thereof is lowered. The yield of products is increased and steam consumption during the process is reduced. Small molecule pigments also dissolve out of carrageen cells during the procedure of enzymatic hydrolysis, thus improving the transparency and purity of the obtained carrageenan. Since alcohol is adopted in dehydration procedure, the water content of the products is greatly reduced. Meanwhile, bactericidal effect of alcohol is favorable to inhibit the breeding of microorganisms during the process, thereby fulfilling the objective of disinfection and bacteriostasis. The above technique has the advantages of high extraction efficiency, high utilization rate of raw materials and high transparency and purity of the obtained carrageenan.

The invention relates to a compound plant-derived pesticide, which is prepared by mixing the following components in percentage by weight: 2-5% of nicotine, 2-5% of wild mugwort oil, 3-8% of cinnamon leaf oil, 5-10% of litsea cubeba oil, 30-40% of flavescent sophora root mixed ethanol extract, 10-15% of Rangoon creeper fruit mixed water extract, 15-20% of emulsifying agent, 1-2% of sodium dodecylsulfate and the balance of water, wherein the flavescent sophora root mixed ethanol extract is obtained by extracting 3 parts by weight of flavescent sophora root, 1 part by weight of triperygium wilfordii, 2 parts by weight of toosendan fruit, 2 parts by weight of hellebore, 2 parts by weight of szechuan aconite, 1 part by weight of cnidium fruit and 1 part by weight of camphor tree seed with 70wt% ethanol solution, and has the relative density of 1.02-1.10; and the Rangoon creeper fruit mixed water extract is obtained by extracting 1 parts by weight of groundsel and 2 parts by weight of Rangoon creeper fruit with water solution, and has the relative density of 1.02-1.10. The compound plant-derived pesticide overcomes the limitations that the most plant derived pesticides are single in pest-killing components and simple in action mechanism, improves the comprehensive prevention and control effect, is quick in acting and long in duration, and avoids the generation of pest pesticide resistance.

The present invention relates to sphaelactone derivatives, their pharmaceutical compositions, a preparation method thereof and application thereof, and especially relates to a sphaelactone derivative pharmaceutical compound which is represented by a formula (I) and is used for treating cancer diseases, pharmaceutical compositions containing an effective amount of the sphaelactone derivative pharmaceutical compound represented by the formula (I) and pharmaceutically acceptable carrier or pharmaceutical compositions with other anti-cancer medicine, a preparation method of a sphaelactone derivative, and application of the sphaelactone derivative in medicine, especially application in preparation of medicines for treating cancer diseases.

The invention relates to application of sphaelactone and a derivative thereof to the treatment of cancers, in particular to application of a medicinal composition of the sphaelactone shown as a formula (I) and the derivative thereof to the treatment of the cancers, a medicinal composition which contains a therapeutically effective amount of a compound (I) and a pharmaceutically acceptable carrier, or a composition of the medicinal composition and other anti-cancer medicaments, a preparation method of the sphaelactone derivative, and application of the sphaelactone and the derivative thereof to medicaments, particularly the preparation of the medicaments for treating the cancers.

The invention discloses a method for manufacturing an inorganic nano-silver mildew-proof antibacterial healthy decorative veneer. The method comprises the following steps: (1) soaking a decorative veneer in aqueous solution of nano-silver, and drying the decorative veneer; (2) coating adhesive containing inorganic nano-silver on the surface of a base material; and (3) applying the decorative veneer on the adhesive on the surface of the base material, and performing compression molding on the veneer. The decorative veneer manufactured according to the method can resist invasion of mildew caused by dampness of the base material to the surface of the decorative veneer, ensures that sheet materials can achieve the effect of mildew proofing and bacteria resistance and has environment-friendly, non-toxic, high-efficiency, stable, long-acting mildew-proof antibacterial effect, has mildew-proof and antibacterial grade reaching the highest 0 level of JIS Z2911 standard, and can effectively improve the domestic living environment for people. The veneer can be used in the fields of building materials, furniture, indoor furnishing and decorating, vehicles and ships and the like. The healthy sheet materials decorated indoors have strong killing effect on epidemical bacillus, trichodermin, stain fungi and the like.

The invention relates to a specificity yelk immune globulin composition for preventing pathogenic bacteria. The composition is prepared by preparing compound antigen, performing supplementary immunization on chicken, preparing crude extract and purifying. The invention further relates to a preparation prepared from the composition. The preparation comprises the following components in percentage by mass: 0.15-0.3% of specificity yelk immune globulin composition for preventing pathogenic bacteria or crude extract of the composition, 0.3-0.6% of povidone K30, 1.0-2.0% of glycerol, 0.3-0.5% of polysorbate, 0.10-0.30% of fresher essence, 0.05-0.1% of edible essence and the balance of distilled water of 100%. The specificity yelk immune globulin composition for preventing pathogenic bacteria and the preparation of the composition are high in antibody activity, high in specificity, good in specificity sterilization effect, free of microecological balance interference, free of adverse irritation on human bodies and high in safety performance.

The invention provides a CD19 targeted CAR (chimeric antigen receptor)-T cell, a preparation method and an application and relates to the field of immune cells. The activation capacity of T cells in CAR-T cell therapy can be improved, the problem of insufficient transfection efficiency can be solved, and the CAR-T cell has a high killing capacity for CD19. The CD19 targeted CAR-T cell expresses a CD19 targeted CAR gene on surface, and the CD19 targeted CAR gene is formed by connecting a single-chain antibody CD19ScFv of CD19, a hinge region and a transmembrane region of CD8, an intracellular signal structure of CD28, an intracellular signal structure of 4-1BB and an intracellular signal structural domain of CD3zeta in series, and the base sequence of the CD19 targeted CAR gene is shown as SEQ ID NO:2.

The invention relates to a chimeric antigen receptor (CAR) and application thereof. The CAR comprises BCMA (B-cell maturation antigen) binding structural domains, transmembrane structural domains, oneor a plurality of co-simulation structural domains and intracellular signal transduction structural domains. The BCMA binding structural domains comprise heavy chain complementary determining regionsHCDR 1-3, and amino acid sequences of the HCDR 1-3 are sequentially shown as SEQ ID NO:1-3.

An agricultural chemical composition for controlling pests including armyworm and bacteria contains berberine (0.5-2.5%) and matrine (0.1-2.0%). Its advantages are high effect, no environmental pollution, and low toxicity to people and livestock.

The invention relates to a composition for killing microbes, which is mainly used for hand antisepsis, surgical hand antisepsis and antisepsis of skins and mucosa and can be also used for killing the microbes at the surfaces of the articles; polyhexamthylene guanidine hydrochloride and ethanol are adopted as main active fungicide ingredients to be combined with a fungicide synergistic agent isopropanol, wherein the concentration of the ethanol is 79-467 g / L, the concentration of the polyhexamthylene guanidine hydrochloride is 0.01-15 g / L and the concentration of the isopropanol is 3-54 g / L; meanwhile, a moisturizer, a stabilizing agent, a thickening agent and other agents can be added as needed to obtain different dosage forms; and the composition has the advantages of good fungicide effect, high speed, no washing, high stability, reduction of skin irritation of the product and functions of protecting and moisturizing skins.

The invention relates to a biogenic composite pork preservative. The biogenic composite pork preservative comprises 4.0-4.8% of a bacterial depressant, 10-14% of a synergist, 27-33% of Chinese herbal medicine extract A, 16-20% of Chinese herbal medicine extract B and the balance water. The bacterial depressant contains nisin, natamycin, lysozyme, chitosan and propolis supercritical CO2 extract. The synergist contains acetic acid, vitamin C, vitamin E and sodium lactate. The Chinese herbal medicine extract A is prepared through extracting grape seeds, garlic, clove, cassia bark, rosemary, pepper and ginger through an ethanol extraction method and then blending the extract products, Origanum vulgare L. essential oil and tea crude extract. The Chinese herbal medicine extract B is prepared through water extraction of konjak, Chinese honeylocust fruits, rheum officinale and pomegranate peel. The biogenic composite pork preservative has a reasonable formula, is safe for eating, can be prepared by simple processes, is convenient for operation in use and has good fresh-keeping effects.

The invention relates to a formula of an aquatic product composite preservative. The formula of the aquatic product composite preservative is characterized in that the aquatic product composite preservative in each liter of distilled water comprises the following components: 0.4 to 0.6 g of muramidase, 2.5 to 3.5 g of tea polyphenol, 16 to 20 g of carboxymethyl chitosan, 10 to 14 g of nisin, 25 to 35 g of potassium sorbate, 17 to 23 g of sodium alginate, 20 to 30 g of lactobacillus, 1.5 to 2.5 g of propolis extracts and 10 to 14 g of spice extracts; and the spice extracting liquid comprises the raw materials: galangal, garlic, onion, cinnamon, clove and rosemary. The aquatic product composite preservative provided by the invention is prepared by compounding biological preservatives, is safe and non-toxic, has simple preparation and application methods, prominent microbial inhibition effect and good preservation effect, can greatly prolong the shelf life of frozen aquatic products, and has important industrial value.

The invention provides a nucleic acid molecule for encoding a chimeric antigen receptor targeting CD22. The chimeric antigen receptor comprises an extracellular region, a transmembrane region and a intracellular signal transduction region, the extracellular region encoded by the nucleic acid molecule comprises a CD22 binding domain, and the CD22 binding domain is amino acid sequence as shown in SEQ ID No. 3. By using flow cytometry, a degranulation analysis experiment and ELISA to detect cell factors secreted by a T cell, a fact that the CAR-T cell has a high lethal effect on B cell lymphoma cells and acute B cell lymphoma cell leukemia cells expressing CD22 and hardly has a lethal effect on cells which do not express CD22, and an off-target effect is prevented effectively is proved. The chimeric antigen receptor CD22scFv-CD8alpha-4-1BB-CD3zeta can be used for treating CD22<+>B cell hematologic neoplasms and is applicable to combined treatment with a CD19 CAR-T cell.

The invention provides a quaternary ammonium salt grafted guanidine salt bactericide and a preparation method thereof. The preparation method comprises the following steps of: firstly, carrying out polycondensation reaction on polyamine and an inorganic acid guanidine salt under the inertia atmosphere to obtain a polyguanidine salt; mixing long chain alkyl tertiary amine with industrial hydrochloric acid and then reacting the mixture with epichlorohydrin in a hydrophilic organic solvent to obtain a homogenous solution, and removing epichlorohydrin and alcohol solvent remaining in the reaction to obtain a quaternary ammonium salt; and grafting the polyguanidine salt with the quaternary ammonium salt in the hydrophilic organic solvent and a pure water solution to obtain the quaternary ammonium salt grafted guanidine salt bactericide. According to the invention, the main chain of the bactericide is provide with a guanidyl positive ion, and a quaternary ammonium positive ion and tertiary amine are introduced in the side chain of the bactericide, so that the density of the positive charge is enlarged, the bactericidal performance is greatly improved, and simultaneously, the bactericide more easily penetrates through microorganism so that cellular contents overflow, bacteria die and are firmly adsorbed, and durable bactericidal property is better. The bactericide disclosed by the invention can be applied to antimicrobial paper, antimicrobial fiber, and antimicrobial paint industries, daily chemicals industry, washing and sterilization treatment of foodstuff, fruits and vegetables, sterilization and disinfection of wastewater in hospitals and the like.

The invention relates to an efficient snail-killing type organic fertilizer produced from tea seed cakes and a preparation method thereof. The efficient snail-killing type organic fertilizer is characterized by being prepared from the following raw materials in percentage by weight: 45 to 60 percent of the tea seed cakes, 25 to 45 percent of chicken manure, 0.5 to 3 percent of phosphate fertilizer, 5 to 20 percent of organic additive, 0.5 to 3 percent of microbial decomposition agent, and 1 to 4 percent of snail-killing synergistic agent. The efficient snail-killing type organic fertilizer has the characteristics of complete nutrients, cultivatable fertility of a common organic fertilizer, can effectively kill paddy field pomacea, leeches, wireworms and the like, is biologically environment-friendly and nuisanceless, has the function of regulating the plant growth, can carry out snail-killing and fertilizing at the same time, simplifies the farming procedures, is convenient to use, saves labor power and materials, and can stimulate the growth of plant; and a contained plant growth regulator can promote the metabolism and the photosynthesis of the plants, promote the root system development, increase new roots, promote the reproductive development, and improve the disease-resistant and degeneration-resistant capacities of paddy.

The invention relates to a CD19-based chimeric antigen receptor and application thereof, in particular to a lentivirus vector material built by a chimeric antigen receptor T (CAR-T) cell technology using a tumor specific target point CD19 as the basis, a method, and application thereof to anti-tumor treatment. The chimeric antigen receptor is formed by serially connecting an antigen combination structure domain, a membrane spaning structure domain, a costimulatory signal conduction region, a CD3 zeta signal conduction structure domain and an inducible suicide fusion structure domain, wherein the antigen combination structure domain is combined with the tumor surface antigen; the tumor surface antigen is CD19. The chimeric antigen receptor is subjected to specific gene transformation on theT cell stimulation signals. Compared with other chimeric antigen receptors, the chimeric antigen receptor provided by the invention has a better reaction effect and higher safety, so that the CAR-T cells have higher immune effects and low side effects; the treatment effect and safety of the CAR-T cells are enhanced.

The invention discloses an organic fertilizer for controlling soil salinization of coastal saline soil and killing underground insects and a preparation method thereof. The organic fertilizer provided by the invention comprises, by weight, 300-500 parts of straw compost, 300-500 parts of humic acid and 100-200 parts of a saline-alkali modifier, wherein the straw compost is composed of tobacco straw, livestock and poultry night soil, high-efficiency composite microbial inoculants and urea; and the saline-alkali modifier is composed of gypsum and an attapulgite powder. The organic fertilizer can be used to raise the fertilizer conservation and water retention capability of soil, prolong fertilizer efficiency and supplement beneficial bacteria in soil. With the application of the organic fertilizer, the harm of underground insects in field can be obviously reduced, underground insects are effectively killed, chemical agent amount is reduced, work and cost are saved, and toxicity and side-effect residual quantity of chemical control is reduced. In addition, after the application of the organic fertilizer, the topsoil of coastal saline soil is loosened, unit weight is decreased and the organic carbon content is increased.

The invention relates to a coupling agent in the field of application and ultrasonic detection and particularly relates to a B ultrasound coupling agent containing traditional Chinese components, which is suitable for detecting wounds or injuries in other forms on the skin surface of a detected part. The B ultrasound coupling agent containing traditional Chinese components is prepared from the following components in percent by mass: 0.25%-0.85% of rheum officinale, 0.30%-0.90% of borneol, 0.45%-0.75% of honeysuckle, 0.20%-0.50% of dandelion, 0.35%-0.65% of wild chrysanthemum flower, 0.10%-0.30% of aloe, 5%-15% of glycerol, 3%-6% of sodium carboxymethyl cellulose, 10%-15% of alcohol and the balance of deionized water. The B ultrasound coupling agent containing traditional Chinese components, which is disclosed by the invention, is novel in scheme, low in cost and simple to prepare, and has a remarkable sterilization effect and cross infection preventing ability by being jointed and combined with a plurality of traditional Chinese medicines with an antibacterial effect.

The invention discloses a method for preparing a low-toxicity marine antifouling paint by applying Cu2O hollow submicrospheres, and belongs to the fields of preparation and application of inorganic functional materials and marine antifouling paints. The method applies the Cu2O hollow submicrospheres singly or the Cu2O hollow submicrospheres embedded with a nontoxic organic antifouling composition as an antifouling composition to prepare the low-toxicity marine antifouling paint; and the antifouling composition, resin base stock, pigment (filler), and solvent are added to a ball mill for grinding to prepare the marine antifouling paint, wherein the antifouling composition consists of the Cu2O hollow submicrospheres with adjustable grain diameter (150-500nm), hollow structures and porous surfaces or the Cu2O hollow submicrospheres embedded with other antifouling compositions. The method has the characteristics that the Cu2O grain diameter reaches nano / micron grade, and grains in the paint are distributed uniformly; and the special appearance of the hollow structures and the porous surfaces can be used for embedding and slowly releasing other nontoxic organic antifouling compositions, reduces Cu2O dosage and has the function of cooperative antifouling so as to prolong the antifouling term, strengthen the antifouling effect and reduce the emission pollution.