The invention belongs to the technical field of pesticides, and particularly relates to a quick-acting environment-friendly agricultural insecticidal acaricide for killing high-resistance pests and application thereof. The acaricide comprises straight chain sodium dodecyl benzene sulfonate, alkyl polyglucoside, sodium di-sec-octyl maleate sulfonate, polyethylene glycol, betaine, a molasses solution, a urea solution, a vinegar solution, dimethyl sulfoxide, a defoaming agent, a fennel extract, proteolytic enzyme, lauryl sodium sulfate, a mint extract, an andrographis paniculata extract and piperonyl butoxide. The insecticidal acaricide can be independently used as an insecticidal acaricide, and can also be used as a pesticide synergist in bactericides, foliar fertilizers, plant growth regulators and herbicides. The disclosed acaricide is low in price, easily available in raw materials, capable of efficiently killing more than 30 kinds of high-resistance pests, safe, environment-friendly,non-toxic or slightly toxic, non-toxic and residue-free to edible vegetables and fruits, and remarkable in economic benefit and social benefit.