The invention discloses a preparing method of tilapia bait. The bait comprises almond powder, flat peach powder, honeysuckle powder, fennel powder, dried orange peel powder, Jamestown weed, mushroom powder, longan powder, fish powder, carrot meal, melon powder, float grass powder, hawthorn powder, powdered glucose, apple powder, diffusant for fish, cracker meal and corn extruded meal, and the percentage adopts weight percentage. The bait has thick aroma, and has thick fishy smell after entering water, and the fishy smell can be rapidly spread, the bait can strongly stimulate the taste bud of tilapia around, so as to ensure that the tilapia can smell the fishy smell from far away and can see that the bait in the water is slowly expanded, the shape of the expanded bait is good and good for the tilapia to swallow the bait, the bait is similar to live bait, and can attract the tilapia to swallow in the shortest time.