The invention relates to an antibacterial down feather and a manufacturing method thereof. The manufacturing method comprises the steps of raw material stem removal, dust and sand removal, precise separation, washing, dehydration, drying, cooling and package, wherein an antibacterial agent comprises the following components in portion by weight: 6 to 10 portions of quaternary ammonium salt A, 1 to 2 portions of modified chitosan, 0.2 to 0.5 portion of silane coupling agent, 0.1 to 0.5 portion of solubilizing agent, and 0.3 to 0.6 portion of dispersing agent. The antibacterial down feather can effectively prevent mildew and attack of insects of the down feather in storage, transportation and use, and particularly when the antibacterial down feather serves as a filling material and is manufactured into articles for daily use, because of the antibacterial and bacteriostatic functions, the corresponding products have obvious functions of resisting bacteria, diminishing inflammation, deodorizing, resisting mildew, relieving itching and converging, and have no stimulation to skins, and the antibacterial rate of the obtained down feather to staphylococcus aureus bacteria reaches up to 99 percent, and after 10 times of washing, the antibacterial rate still reaches 92 percent.