502results about How to "Promote Microecological Balance" patented technology

The invention discloses an enterococcus faecium and application thereof. An enterococcus faecium strain provided by the invention is enterococcus faecium suppressant 80, and the collection number is CGMCC No.5058. As proved by experiments, the enterococcus faecium suppressant 80 is obtained by separating, identifying and screening; and the enterococcus faecium suppressant 80 has high resistance, high bacterial resistance and probiotic characteristics, and can be taken as an additive for preparing animal feeds; and the animals include but is not limited to various animals such as pigs, cows, sheep, chicken and the like. The feeds have similar functions to antibiotic feeds, but do not have the side effects of the antibiotic feeds.

The invention discloses a composite capable of promoting skin micro-ecological balance and a preparation method of the composite. The composite comprises the following main ingredients of skin prebiotics alpha-glucan oligosaccharide, fructooligosaccharide, beta-glucan, plant extract, yeast extract and natural antibacterial agent leuconostoc/ radish root fermentation product filter liquor. According to the composite, micro-ecological balance of the skin can be fundamentally adjusted, skin health is kept, the defects that traditional cosmetics or skin care products cure symptoms, not diseases and are prone to generating side effects are overcome, and the composite has the moisture preservation, softening and tightening multi repair effects, and provides a new thought for solving the skin problems. The preparation method of the composite is simple and practicable, and active ingredients of the functional components are kept to the maximum degree by reasonably controlling reaction steps and mixing temperature.

The invention relates to an enzyme preparation method. The enzyme is prepared by mixing, fermenting and extracting the following raw materials in parts by weight: 100 to 200 parts of natural organic fruits and vegetables, 100 to 200 parts of traditional Chinese herbals, 5 to 10 parts of clean water, 1 to 30 parts of probiotics, 500 to 2500 parts of edible sugar, and 100 to 400 parts of salt. Specifically, the preparation method comprises the following steps: picking, weighing, and washing natural organic fruits and vegetables and traditional Chinese herbals, drying natural organic fruits and vegetables and traditional Chinese herbals in the air, putting natural organic fruits and vegetables and traditional Chinese herbals into an extraction tank; after all raw materials have been added into the extraction tank, adding clean water, white sugar, salt, and probiotics, evenly mixing; carrying out primary fermentation for 7 to 15 days, naturally extracting a liquid containing effective components, controlling the indoor temperature in a range of 10 to 25 DEG C; filtering to obtain an enzyme primary liquid; delivering the obtained enzyme primary liquid into a tank body in a fermentation aging chamber by a liquid pump through a pipeline, keeping a constant room temperature (25-37 DEG C) of the fermentation aging chamber; and carrying out fermentation aging for 240 to 480 days to obtain the liquid enzyme stock solution.

The invention provides bacillus subtilis K018 which is strong in stress resistance, excellent in probiotics function and safe and reliable, a biological feed additive containing the bacillus subtilis K018 and use thereof. The bacillus subtilis K018 provided by the invention can resist artificial gastric acid, cholate, artificial intestinal juice and high temperature, has stronger inhibiting effects to pathogenic staphylococcus aureus, escherichia coli, salmonella enteritidis and the like in the intestinal tract, is stronger in amylase and cellulase generating capacity, and can degrade starch and cellulose.

The invention relates to a fruit plant enzyme drink and a preparation method thereof. The fruit plant enzyme drink comprises the following formula components: 4% of white granulated sugar, 4% of honey, 4% of maltose and the balance being plant fruit raw materials, wherein the plant fruit raw materials comprise one or a plurality of green plum, smoked plum, mulberries, blueberries, raspberries, apples, peaches, red dates, grapes and wild jujubes. The preparation method of the fruit plant enzyme drink comprises the following steps: examining and receiving the raw materials, washing, fermenting, sterilizing and filling. The invention provides the drink with the health function. The drink has the functions of reducing the intestinal tract load, activating the healthy circulation system, adjusting the microecological balance, enhancing the human body cell vitality, purifying the blood, promoting the metabolism, inspiring the human body cell vitality, enhancing the metabolism function and enhancing the body rejuvenation.

The invention provides a Bacillus coagulans HEW-B379 with a probiotic effect. The above strain is named as HEW-B379, and the preservation number of the strain is CGMCC No.12553. The Bacillus coagulans HEW-B379 has a substantial probiotic property, and can effectively inhibit growth breeding of enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, salmonella, Shigella, Proteus species, Shewanella putrefaciens and Pseudomonas aeruginosa. The Bacillus coagulans HEW-B379 has strong stress resistance, can resist high temperature and simulated gastric juice and simulate bile salt environment, can keep the survival rate of 99-100%, and can effectively adjust microbial balance of animal intestinal tracts, inhibit growth of harmful microbes, promote nutrition absorption of animals, improve the conversion rate of a feed and improve the productivity of the animals.

The invention discloses a clostridium butyricum having a diarrhea treating effect. The strain is named as clostridium butyricum Cb-2 which is conserved in China Center for Type Culture Collection with the conservation number of CCTCC M2011384, in November 9th, 2011. A test proves that the clostridium butyricum disclosed by the invention has strong acid producing capability especially butyric acidproducing capability; and the clostridium butyricum has obvious effects on diseases, such as intestinal tract flora infection, acute and chronic diarrhea, irritable bowel syndrome and non-ulcer dyspepsia, caused by various reasons, and has no toxicity and harm. Thus, the clostridium butyricum or clostridium butyricum powder disclosed by the invention is used as a raw material for preparing a micro-ecological preparation and used for replacing sodium butyrate to be applied; and when a specific application is carried out, a clostridium butyricum preparation can be singly used as an active component to be prepared into the micro-ecological preparation together with corn starch, or, the clostridium butyricum preparation is mixed with at least one of other bacterial preparations and the corn starch to prepare a composite micro-ecological preparation.

The invention discloses a probiotics fermentation Chinese medicinal herb composite feed additive used for preventing and treating diarrhea of delivery room suckling piglets and weaning conservation piglets. According to laboratory repeated fermentation experiments and actual using effect of products at a plurality of scaled pig farms, a screened and determined formula is as follows: bacillus cereus inoculum size 2-4%, bacillus subtilis inoculum size 2-4%, Chinese medicinal herb inventory 8-20% (main ingredients of which are the root of Chinese pulsatilla, myrobalan, liquorice and schisandra chinensis), and performing liquid state fermentation and then drying. The probiotics fermentation Chinese medicinal herb composite feed additive used for preventing and treating diarrhea of delivery room suckling piglets and weaning conservation piglets can effectively resolve the problem of piglet diarrhea during scaled pig farming process, effectively improve organism immunity and disease resistance capability of piglets, adjust animal enteric micro-ecology balance and repair intestine mucosa damage, and has the effects of improving weaning piglet daily gain by 6%-12% and feed use ratio by 10%-15% and lowering diarrhea rate by 25%-35%. The probiotics fermentation Chinese medicinal herb composite feed additive used for preventing and treating diarrhea of delivery room suckling piglets and weaning conservation piglets is an ideal green environment-friendly safe and novel feed addictive for replacing antibiotic medicines in diarrhea controlling.

The invention relates to a compound feed addictive with bacteria and oxidation resisting functions. The compound feed addictive is prepared from the following components in percentage by weight: 10-15% of antibacterial peptide, 10-20% of propolis, 10-25% of fructus ligustri lucidi extractant, 15-30% of lyophilized bacillus subtilis bacteria freeze-dried powder, and 40-70% of solid fermentation culture of ganoderma lucidum bacteria, wherein the bacillus subtilis is CGMCC (China General Microbiological Culture Collection Center) 6012. The compound feed addictive disclosed by the invention can efficiently reduce the usage amount of antibiotic medicine in feeding animals, the safety of meat products of animals is improved, the utilization efficiency of the feed of the animals is improved, the immunity of the animals is improved, and the feeding efficiency is improved.

The present invention belongs to the field of microbial technology, relates to a liquid-solid combined fermentation process of probiotics using bifidobacterium as main raw material, mainly includes the following steps: making bifidobacterium, acidophilic lactobacillus and thermophilic streptococcus respectively undergo the two stages of stock 1-3 order liquid culture and solid culture, the weightratio of three bacteria powders in the preparation is 50-80:10-15:5-16. Said solid culture includes the following procedures: firstly, sterilizing, inoculating 20-30% of tertiary culture, placing them into plastic bag, vacuum-pumping, injection anaerobic sterile N2 into said non-toxic sterile plastic bag, and adding bifiobacterium into solid culture medium, and promoting live bacterium multiplication to 10 to the power 8-10 to the power 9 order, and can prolong its storage time.

The invention provides a composite bacillus preparation containing three strains, a preparation method of the composite bacillus preparation and application of the composite bacillus preparation to ecological breeding, and belongs to the technical field of microorganism and feed additives. Bacillus coagulans, butyric acid clostridia and bacillus megaterium are used as fermentative strains respectively, and large-scale, low-cost and high-density fermentation production of spores is conducted through step-by-step magnification; three live bacterium preparations are prepared by mixing bacterial sludge with the spores and dry starch; the three live bacterium preparations are matched according to the equal weight ratio, the composite bacillus preparation is obtained, and the concentration of the spores reaches more than 1*108-109 cfu/g; the composite bacillus preparation serves as a microorganism and feed additive to be added to farmed animal feed according to the mass ratio ranging from 0.02% to 0.5%. The composite bacillus preparation is simple and stable in process, low in cost, high in spore content and environment tolerance, easy to store and high in survival rate; the purposes of composition and synergism are achieved through the collaborative supplementary effects of different bacilli in the aspect of micro-ecology adjustment, growth of farmed animals is promoted, the feed utilization rate and feed rewards are increased, diarrhea is prevented and reduced, and the breeding ecological environment is improved.

The invention belongs to the technical field of feed, and particularly relates to compound feed for improving the production performance of laying hen and the egg quality. The compound feed consists of corn, lard oil, soybean meal, rape seed cake, peanut meal, gunite bran, mountain flour, calcium hydrophosphate, lysine, methionine, liquid phytase, liquid xylanase and premix. The compound feed provided by the invention can obviously improve the production performance of laying hen and improve the laying rate and the egg quality, and can increase the weight of a single egg and reduce the damage rate of eggs; and moreover, after being eaten by the laying hen, the compound feed can promote digestive absorption, improve the feed conversion rate and the feed-gain ratio, and increase the economic benefits of laying hen farming.

The invention provides bacillus subtilis (Bacillussubtilis) shou003, an anti-vibrio protein and a preparation method and applications of the bacillus subtilis shou003 and the anti-vibrio protein. The preparation method of the anti-vibrio protein comprises the following steps: screening out bacillus subtilis shou003 (preserved in China Center for Type Culture Collection with a number of CCTCC No.: M2013571 on November 13, 2013) from intestinal tract of a healthy large yellow croaker; and then extracting the anti-vibrio protein from the fermentation broth of bacillus subtilis shou003, wherein the amino acid sequence of the anti-vibrio protein is shown as SEQ ID No.: 1. The bacillus subtilis shou003 and the anti-vibrio protein show good effects on inhibiting aquatic pathogenic bacteria, in particular pathogenic vibrio; in addition, the bacillus subtilis shou003 shows outstanding tolerance to temperature, NaCl, gastric juice, intestinal juice and cholate and can be widely applied to the prevention of germs during aquaculture; on that basis, the optimal fermentation culture method of the bacillus subtilis shou003, and a preparation method of the anti-vibrio protein are provided.

The invention discloses a saline-alkali soil modified microbial inoculum and a preparation method thereof. The microbial inoculum comprises, by weight, 10-30 parts of bacillus megatherium HB bacteriapowder, 35-65 parts of phosphorus tailings powder, 12-18 parts of phosphogypsum, 8-25 parts of sugar refinery wastes, 6-26 parts of seaweed residue and 1-8 parts of mannitol. Components of the saline-alkali soil modified microbial inoculum have synergistic interactions with each other to enhance effects, if any one of the components lacks, expected effects cannot be reached, and only when the saline-alkali soil modified microbial inoculum with whole formulas is adopted and 30-80 kg of the microbial inoculum is used per mu, can the effects of increasing fertilizer utilization rate and crop yields, and improving quality and saline-alkali lands be achieved.