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536 results about "Enterococcus faecium" patented technology
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Enterococcus faecium is a Gram-positive, alpha-hemolytic or non-hemolytic bacterium in the genus Enterococcus. It can be commensal (innocuous, coexisting organism) in the gastrointestinal tract of humans and animals, but it may also be pathogenic, causing diseases such as neonatal meningitis or endocarditis.
Treatment of irritable bowel syndrome using probiotic composition
Owner:COBB & ASSOCS
Broad-Spectrum Antibacterial and Antifungal Activity of Lactobacillus Johnsonii D115
The present invention demonstrated the potential use of Lactobacillus johnsonii D115 as a probiotic, as a prophylactic agent or as a surface treatment of materials against human and animal pathogens such as Brachyspira pilosicoli, Brachyspira hyodysenteriae, Shigella sonnei, Vibrio cholera, Vibrio parahaemolyticus, Campylobacter jejuni, Streptococcus pneumoniae, Enterococcus faecalis, Enterococcus faecium, Clostridium perfringens, Yersinia enterocolitica, Escherichia coli, Klebbsiella pneumoniae, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Aspergillus niger and Fusarium chlamydosporum. The proteineous antimicrobial compound was partially characterized and found to be heat tolerant up to 121° C. for 15 min, and acid tolerant up to pH1 for 30 min at 40° C. The compound is also stable to enzymatic digestion, being able to retain more than 60% antimicrobial activity when treated with pepsin and trypsin.
Owner:KEMIN IND INC
Micro-ecological preparation and application thereof
The invention relates to a micro-ecological preparation and the application thereof, in particular to a micro-ecological preparation containing a variety of probiotics and the application thereof. The micro-ecological preparation contains any three or four of the components including CGMCC No. 2383 bacillus licheniformis powder, bacillus subtilis powder, CGMCC No. 2386 enterococcus faecalis powder, lactobacillus acidophilus powder, and CGMCC No. 2388 saccharomyces cerevisiae. The micro-ecological preparation has content of live bacteria, has the adversity resistance such as gastric acid resistance, bile salt resistance, high-temperature resistance, common antibiotic resistance, and the like and the probiotic functions of producing acid and enzyme and resisting pathogenic bacteria. The variety and the proportion of the probiotics and carrier can be determined according to different kinds of animals and different animal growth phase. The micro-ecological preparation can improve the feed utilization efficiency, increase the yield of meat, eggs and milk, promote the growth of the animal, improve the immunity and the disease resistance of the animal, replace the antibiotic and improve the quality of the animal product.
Owner:BEIJING DABEINONG TECH GRP CO LTD +1
Enterococcus faecium ANSE228 and application thereof
ActiveCN102031235AIncrease production capacityReduce the death rateAntibacterial agentsBacteriaEscherichia coliStaphylococcus cohnii
The invention provides an Enterococcus faecium ANSE228 of which the collection number is CGMCC No.4082. The invention also provides application of the Enterococcus faecium ANSE228 to inhibition of salmonella pullorum and / or Escherichia coli and / or Staphylococcus aureus. The Enterococcus faecium ANSE228 is obtained by processes of repeated separation, purification, rejuvenation and the like, and has high biological activity, obvious probiotic property, high adversity resistance and the like. The invention also provides a microecological agent which contains the Enterococcus faecium ANSE228. When the microecological agent is added into drinking water and / or feeds for breeding animals, the Enterococcus faecium ANSE228 can be quickly activated and reproduced and a dominant beneficial flora can be formed after the Enterococcus faecium ANSE228 is fed into intestinal canals of the animals, and the Enterococcus faecium ANSE228 has the effects of reducing a harmful flora in the intestinal canals, adjusting microecological balance of the intestinal canals, substituting for medicaments such as antibiotic and the like, and improving weight increment of the animals and the utilization rate of the feeds.
Owner:科润生科技发展有限公司
Treatment of autism using probiotic composition
A method of treating autism using a probiotic composition including the bacilli (1) Bacillus subtilis, (2) Bacillus coagulans, and (3) Enterococcus faecium. The composition may further include a carrier medium, such as fructo-oligo-saccharides (FOS), as incorporated in a dose form such as a pill, capsule, powder or sachet. The compositions of the invention may be usefully employed as health or nutritional supplements, food additives, or therapeutic agents for combating a wide variety of physiological disorders.
Owner:COBB & ASSOCS
Micro-ecological fruit-vegetable enzyme preparation method
InactiveCN103859404ARich sourcesLess investmentFood ingredient functionsFood preparationCulture fluidAdditive ingredient
The invention discloses a micro-ecological fruit-vegetable enzyme preparation method. The method comprises the steps of culturing 4 to 6 of six probiotics including lactobacillus acidophilus, bacillus coagulans, bacillus natto, saccharomyces cerevisiae, enterococcus faecium and bifidobacterium in a culture medium comprising ingredients such as brown sugar, liquorice and fresh sweet potato, and then culturing the probiotics through a diluted culture liquid containing brown sugar and liquorice in an activating manner to prepare a micro-ecological preparation, wherein the prepared micro-ecological preparation is a fermenting agent which is used for fermenting fruit pulp of fresh fruit and vegetable and traditional Chinese herbs. The microorganism enzyme in the ecological preparation and the natural vegetable enzyme in fruits, vegetables and Chinese herbs are compounded into a composite enzyme supplement by utilizing a micro-ecological fermentation technology, i.e. the micro-ecological fruit-vegetable enzyme. The micro-ecological fruit-vegetable enzyme can be made into a liquid type or a solid type (powder). An ideal effect is achieved in the fields such as medicine, healthcare and beauty.
Owner:JUNZHU DALIAN BIOLOGICAL IND CO LTD
Enterococcus faecium for feeding and applications thereof
InactiveCN102304483AConducive to preservationLong storage timePowder deliveryBacteriaFreeze-dryingAntibiotic Y
The invention relates to enterococcus faecium for feeding as well as a freeze-drying fungicide and applications thereof, and discloses enterococcus faecium LAB12 CGMCC (China General Microbiological Culture Collection Center) No.4847 which is grampositive cocci, has no spores, grows well on an MRS agar plate, forms a round bacterial colony with the diameter of 0.5-1mm within 48 hours and is used for feeding, and the bacterial colony is round, smooth and upheaved, and is a shape of grey white dewdrop; the enterococcus faecium grows in a facultative anaerobic condition; the growth temperature range is 10 DEG C-45 DEG C; the optimum growth temperature is 30 DEG C-40 DEG C; and the growth pH value is 4-10, and the optimum pH value is 6.0. The freeze-drying fungicide formed by the bacterial strain is nontoxic and harmless, is gastric juice resistant, is cholate resistant, has a high inhibitory effect for multiple harmful bacteria, has a long quality guarantee period, can be widely applied to birds and livestock breeding to strengthen the animal disease-resistant capability, and is expected to be the substitution of antibiotics for feeding.
Owner:北京金泰得生物科技股份有限公司
Affinity purified human polyclonal antibodies and methods of making and using them
The present invention describes a method for treating, removing or preventing a bacterial infection, which method comprises administering to a human suffering, suspected of suffering or at risk of suffering from Staphylococcus aureus (S. aureus) infection, a Streptococcus infection, Escherichia coli (E. coli) infection, Pseudomonas aeruginosa (P. aeruginosa) infection, Acinetobacter baumannii (A. baumannii) infection, Enterococcus faecium (E. faecium) infection and / or Clostridium difficile (C. difficile) infection, an effective amount of human polyclonal antibodies affinity purified from a human blood sample with an antigenic preparation comprising cellular and / or secreted antigen(s) from bacterial cells selected from S. aureus, a Streptococcus, E. coli, P. aeruginosa, A. baumannii, E. faecium, C. difficile or a combination thereof, and optionally, wherein said affinity purified human polyclonal antibodies are purified (e.g., as made more concentrated as compared to the starting or unpurified material) relative to the same human polyclonal antibodies in the unpurified or non-affinity-purified human blood sample, e.g., intravenous immunoglobulin (IVIG) sample, and / or also optionally, wherein said affinity purified human polyclonal antibodies are specific for the bacterial antigens used in the affinity purification, and / or further optionally wherein the affinity purified human polyclonal antibodies are substantially free of human antibodies that specifically bind to non-bacterial antigens in the human blood sample. Pharmaceutical compositions for treating bacterial infections, comprising an effective amount of human polyclonal antibodies affinity purified from a human blood sample with an antigenic preparation comprising cellular and / or secreted antigen(s) from S. aureus, Streptococcus, E. coli, P. aeruginosa, A. baumannii, E. faecium, C. difficile or a combination thereof, are also provided.
Owner:SCANTIBODIES LAB
Enterococcus faecium EF08 as well as feed additive and feed containing enterococcus faecium EF08
ActiveCN104195075AReduce excessive consumptionHigh feed conversionBacteriaMicroorganism based processesBiotechnologyAnimal product
The invention discloses an enterococcus faecium EF08 as well as a feed additive and a feed containing the enterococcus faecium EF08. The enterococcus faecium EF08 is collected with the number of CGMCC (China General Microbiological Culture Collection Center) No.5549 in CGMCC. The feed additive disclosed by the invention can be used for reducing the feed excessively consumed by harmful bacteria in intestines by virtue of an equalizing effect of bacteria for equalizing the intestinal environment, furthermore, the conversion rate of the feed for economic animals is increased, the feed for the economic animals can be effectively converted into a primary animal product with an economic value, and then, the effect of increasing the economic values of the economic animals can be improved.
Owner:SYNBIO TECH
Composite microorganism additive agent for milk cattle feed stuff and method of preparing the same
InactiveCN101253934AReduce somatic cell countIncrease milk productionFungiBacteriaDiseaseBiotechnology
The invention discloses a compound microorganism additive for milk cow feed. The microorganism additive consists of fermentation product mixture of natto bacillus subtilis, enterococcus faecium and saccharomyces cerevisiae, wherein the total number of bacteria is more than 10<9>cfu / g. Long-term repeating animal experiments show that three microorganisms in the compound microorganism additive can develop synergy, thereby significantly improving the milk yield of the milk cow, fat percentage in the milk and milk protein yield, significantly reducing the somatic number of the milk cow in lactation period and the disease probability of mastitis and subclinical mastitis, and significantly enhancing the autoimmunity of the milk cow organism. The compound microorganism additive has the advantages of low cost, significant effect and good application and market prospects.
Owner:INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
InactiveUS20040185478A1Reduce usageDetermine rapidly the bacterial resistance to antibioticsMicrobiological testing/measurementFermentationBacteroidesNeisseria meningitidis
DNA-based methods employing amplification primers or probes for detecting, identifying, and quantifying in a test sample DNA from (i) any bacterium, (ii) the species Streptococcus agalactiae, Staphylococcus saprophyticus, Enterococcus faecium, Neisseria meningitidis, Listeria monocytogenes and Candida albicans, and (iii) any species of the genera Streptococcus, Staphylococcus, Enterococcus, Neisseria and Candida are disclosed. DNA-based methods employing amplification primers or probes for detecting, identifying, and quantifying in a test sample antibiotic resistance genes selected from the group consisting of blatem, blarob, blashv, blaoxa, blaZ, aadB, aacC1, aacC2, aacC3, aacA4, aac6'-lla, ermA, ermB, ermC, mecA, vanA, vanB, vanC, satA, aac(6')-aph(2''), aad(6'), vat, vga, msrA, sul and int are also disclosed. The above microbial species, genera and resistance genes are all clinically relevant and commonly encountered in a variety of clinical specimens. These DNA-based assays are rapid, accurate and can be used in clinical microbiology laboratories for routine diagnosis. These novel diagnostic tools should be useful to improve the speed and accuracy of diagnosis of microbial infections, thereby allowing more effective treatments. Diagnostic kits for (i) the universal detection and quantification of bacteria, and / or (ii) the detection, identification and quantification of the above-mentioned bacterial and fungal species and / or genera, and / or (iii) the detection, identification and quantification of the above-mentioned antibiotic resistance genes are also claimed.
Owner:GENEOHM SCI CANADA
Microbial feed additive liquid agent for pig and preparation technique
InactiveCN101341927APromote digestion and absorptionImprove conversion rateAnimal feeding stuffBacillus licheniformisNutrient broth
The invention discloses microbial feed additive used for pigs, and a preparation process thereof; the prescription is as follows: 1 portion of bacillus licheniformis, 1 portion of bacillus subtilis, 2 portions of bifidobacterium, 2 portions of enterococcus faecalis, 2 portions of enterococcus faecium, 1 portion of boas-oppler bacillus, 1 portion of lactobacillus casei, 1 portion of lactobacillus lactis, 1 portion of plant lactobacillus, 1 portion of candida utilis, 1 portion of saccharomyces cerevisiae, 80kg to 100kg of panela, 3kg to 5kg of salt, 5kg to 8kg of vinegar, 3kg to 5kg of lysine, 1kg to 2kg of methionine, and 1000L of pure water; the weight of each portion of strain is 2g to 5g. The preparation process is as follows: strain preparation, nutrient broth preparation, inoculated fermentation and check. The invention reduces the quantity of the used individual strain, and the strain quality is safe and reliable. The production process is simple and feasible, the effect is obvious; therefore, the invention has promotion value.
Owner:罗志诣
Probiotic mixture intended for monogastric animals to control intestinal flora populations
InactiveUS6841149B1Good curative effectAssist in growth and activityBiocideBacteriaBiotechnologyBacteroides
A mixture of probiotics effective to reduce the contamination of enteric bacteria in humans and other monogastric animals. The mixture of probiotics includes one or more acid-producing bacteria strains and one or more yeast strains, and may advantageously be supplemented with a source of nutrients, such as prebiotics including fructo-oligosaccharides. In a preferred embodiment, said one or more bacteria strains contain Enterococcus faecium strain NCIMB #10415, and said one or more yeast strains contain NCYC #47 and CNCM I-1079.
Owner:MAYO FOUND FOR MEDICAL EDUCATION & RES +1
Screening and application of probiotic Enterococcus faecium
ActiveCN102747003AGood antibacterial effectImprove securityAntibacterial agentsBacteriaDiseaseSynechococcus
The invention belongs to the technical field of veterinary microbial additive preparation, and specifically relates to a strain of separated and screened Enterococcus faecium providing significant bacterial inhibition effects for common enteropathogenic bacteria such as staphylococcus aureus, escherichia coli and salmonella in breeding animals, and an application thereof. The probiotic Enterococcus faecium of the present invention is characterized in that: the strain is Enterococcus faecium HDRsEf1, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CCTCC NO:M2011031. The probiotic Enterococcus faecium of the present invention has characteristics of fast growth, high acid production capacity, strong stress resistance, safety, disease resistance and growth promotion, and can be used as the microbial feed additive for livestock and poultry feeds.
Owner:武汉华大瑞尔科技有限公司 +1
Kit for quickly detecting 15 pneumonia pathogenic bacteria
ActiveCN107338315AMicrobiological testing/measurementMicroorganism based processesBacteroidesStaphylococcus aureus
The invention discloses a kit for quickly detecting 15 pneumonia pathogenic bacteria. The kit can detect streptococcus pneumoniae, staphylococcus aureus, haemophilus influenzae, mycoplasma pneumoniae, pseudomonas aeruginosa, baumanii, enterococcus faecalis, enterococcus faecium, klebsiella pneumoniae, escherichia coli, enterobacter cloacae, stenotrophomonas maltophilia, burkholderia cepacia, legionella pneumophila and chlamydia pneumoniae which cover clinically common pneumonia pathogenic bacteria difficult to culture. 16S rDNA and specific gene sequences corresponding to the pneumonia pathogenic bacteria are detected by combining gene chips with multiple asymmetric PCR reactions, and the categories of the bacteria in a to-be-detected sample are identified in genus and species. The kit makes up for the defect that current clinical detection of pneumonia pathogenic bacteria is not in time or comprehensive and a novel detection means for early diagnosis and early treatment of patients suffering from pneumonia is provided.
Owner:GENERAL HOSPITAL OF PLA +1
Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
InactiveUS20060263810A1Reduce usageDetermine rapidly the bacterial resistance to antibioticsSugar derivativesMicrobiological testing/measurementNeisseria meningitidisListeria monocytogenes
Owner:GENEOHM SCI CANADA
Method for preparing antibiotic substituent for feed by probiotic asynchronous fermentation of Chinese herbal medicine
The invention discloses a method for preparing antibiotic substituent for feed by probiotic asynchronous fermentation of Chinese herbal medicine, belonging to the field of food nutrition and the technical fields of microorganisms and feed. The method comprises the following steps: extracting Chinese herbal medicine by adopting asynchronous fermentation and enzymolysis technologies, namely adding water and nutritive salt in proportion to prepare a Chinese herbal medicine fermentation liquid after a plurality of Chinese herbal medicines are subjected to impurity removal and super-micro smashing; firstly inoculating Aspergillus niger for aerobic fermentation, thus cellulose and pectinase are prepared; and then inoculating enterococcus faecium and lactobacillus plantarum for anaerobic fermentation; and then carrying out enzymolysis on damage cell walls, and dissolving out active substances of saponin, alkaloid, phytosterol, enzymic preparations, organic acid and the like. A plate-and-frame filter press is adopted to filter the fermentation liquid, the filtration liquid is directly subjected to low-temperature spray drying to prepare antibiotic substituent powder A; a filtration cake is dried by adopting a roller drier to prepare antibiotic substituent powder B, and the power A and the powder B are mixed and formulated in proportion to replace fermented enzymolysis Chinese herbal medicine extracts of antibiotic for feed.
Owner:河南安信生物科技股份有限公司 +1
Enterococcus faecium and application thereof
Owner:北京龙科方舟生物工程技术有限公司
Compound biological strain for feed and preparation method of fermented feed
InactiveCN105483040AInhibition of reproductionEliminate odorFungiBacteriaBiotechnologyBacterial counts
The invention provides a compound biological strain for feed. The compound biological strain comprises enterococcus faecalis, lactobacilli, saccharomycetes, bacillus subtilis and bacillus coagulans, wherein the bacterial count ratio of the enterococcus faecalis to the lactobacilli to the saccharomycetes to the bacillus subtilis to the bacillus coagulans is (0.5 to 1.5):(0.5 to 1.5):(0.1 to 0.5):(4 to 6):(0.5 to 1.5). The compound biological strain for feed and a preparation method of fermented feed provided by the invention have the advantages that the release of crude protein and amino acid in coarse feed can be promoted, and the animal absorption is facilitated.
Owner:河南世纪天缘生态科技有限公司
Treatment of autism using probiotic composition
A method of treating autism using a probiotic composition including the bacilli (1) Bacillus subtilis, (2) Bacillus coagulans, and (3) Enterococcus faecium. The composition may further include a carrier medium, such as fructo-oligo-saccharides (FOS), as incorporated in a dose form such as a pill, capsule, powder or sachet. The compositions of the invention may be usefully employed as health or nutritional supplements, food additives, or therapeutic agents for combating a wide variety of physiological disorders.
Owner:COBB & ASSOCS
Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
InactiveUS20030049636A1Low costRapid positioningMicrobiological testing/measurementFermentationBacteroidesNeisseria meningitidis
DNA-based methods employing amplification primers or probes for detecting, identifying, and quantifying in a test sample DNA from (i) any bacterium, (ii) the species Streptococcus agalactiae, Staphylococcus saprophyticus, Enterococcus faecium, Neisseria meningitidis, Listeria monocytogenes and Candida albicans, and (iii) any species of the genera Streptococcus, Staphylococcus, Enterococcus, Neisseria and Candida are disclosed. DNA-based methods employing amplification primers or probes for detecting, identifying, and quantifying in a test sample antibiotic resistance genes selected from the group consisting of blatem, blarob, blashv, blaoxa, blaZ, aadB, aacC1, aacC2, aacC3, aacA4, aac6'-lla, ermA, ermB, ermC, mecA, vanA, vanB, vanC, satA, aac(6')-aph(2''), aad(6'), vat, vga, msrA, sul and int are also disclosed. The above microbial species, genera and resistance genes are all clinically relevant and commonly encountered in a variety of clinical specimens. These DNA-based assays are rapid, accurate and can be used in clinical microbiology laboratories for routine diagnosis. These novel diagnostic tools should be useful to improve the speed and accuracy of diagnosis of microbial infections, thereby allowing more effective treatments. Diagnostic kits for (i) the universal detection and quantification of bacteria, and / or (ii) the detection, identification and quantification of the above-mentioned bacterial and fungal species and / or genera, and / or (iii) the detection, identification and quantification of the above-mentioned antibiotic resistance genes are also claimed.
Owner:BERGERON MICHEL G +3
A kind of enterococcus faecium and preparation method thereof
ActiveCN103865846BTo promote metabolismPromote growthBacteriaMicroorganism based processesEcological environmentAnimal feces
The invention discloses enterococcus faecium and a preparation method thereof, relates to the field of microorganisms, and particularly relates to the enterococcus faecium and application thereof. A bacterium sample is acquired from the rumen of a slaughtered goat, subjected to directive breeding at the temperature of 32-42 DEG C under an anaerobic and photophobic condition, and separated and purified by use of a repeated divisional agar streak plate method so as to obtain enterococcus faecium L-01. The enterococcus faecium L-01 can be used as an animal microorganism fodder additive, has the effects of effectively inhibiting pathogenic bacterium in animal digestive tract, improving the micro-ecological environment of the digestive tract and enhancing the immunologic function of an animal body, can be used as a substitute product of antibiotics and can be used for effectively solving the existing serious situation.
Owner:江苏绿牧生态环保有限公司
Probiotic composition useful for dietary augmentation and/or combating disease states and adverse physiological conditions
A probiotic composition including the bacilli (1) Bacillus subtilis, (2) Bacillus coagulans, and (3) Enterococcus faecium. The composition may further include a carrier medium, such as fructo-oligo-saccharides (FOS), as incorporated in a dose form such as a pill, capsule, powder or sachet. The compositions of the disclosure may be usefully employed as health or nutritional supplements, food additives, or therapeutic agents for combating a wide variety of physiological disorders, such as irritable bowel syndrome, autism, and fibromyalgia.
Owner:COBB & ASSOCS
Composite microecological preparation and preparation method thereof
ActiveCN105462872AGood cooperation and symbiosisImprove immune activityFungiBacteriaBiotechnologyStaphylococcus lactis
The invention provides a composite active bacterial preparation which is used for aquatic products and has the water improving and fertilizing functions and a preparation method thereof by applying a modern bioengineering technology mainly for the problem that in existing aquaculture, the water quality of a pond is poor. The composite microecological preparation is prepared by mixing bacillus subtilis, bacillus megatherium, bacillus amyloliquefaciens, bacillus licheniformis, enterococcus faecium, lactobacillus brevis, lactobacillus plantarum, lactococcus lactis and saccharomyces cerevisiae through liquid-submerged fermentation. In the liquid preparation, microorganisms are in a dormant state and are stable and easy to preserve, after the preparation is put into water, the dormant strains can quickly resuscitate, harmful substances such as nitrite nitrogen and ammonia nitrogen in the water are quickly decomposed, good algae growth is facilitated, harmful algae reproduction is inhibited, and therefore the purpose of improving the water quality of the culture water is achieved.
Owner:BEIJING DABEINONG TECH GRP CO LTD +2
Compositions and methods useful for modulating immunity, enhancing vaccine efficacy, decreasing morbidity associated with chronic FHV-1 infections, and preventing or treating conjunctivitis
InactiveUS20070280964A1Enhance vaccine efficacyReduce morbidityBiocideSenses disorderTreated animalMicrobiology
Compositions and methods useful for modulating immunity, enhancing vaccine efficacy, decreasing morbidity associated with chronic FHV-1 infections, and / or preventing or treating conjunctivitis in animals. The compositions contain effective amounts of probiotic Enterococcus bacteria and the methods involve administering such compositions to animals alone, in supplements, or in food compositions in amounts suitable for the intended purpose. In certain embodiments, the probiotic is Enterococcus faecium strain NCIMB 10415 (SF68) and the animal is a feline.
Owner:NESTEC SA
Method for preparing complete feed through probiotics asynchronous high-low-moisture fermentation
InactiveCN102599329AHigh nutritional valueHigh oligopeptide contentAnimal feeding stuffAccessory food factorsBiotechnologyBacillus licheniformis
The invention discloses a method for preparing complete feed through probiotics asynchronous high-low-moisture fermentation and belongs to the technical field of microbial feed. The method comprises the following concrete steps that: firstly, protein feed is subjected to impurity removal and crushing, nutrition substances are added, mixed and stirred with clean water, and the moisture content is regulated to 60 to 70 percent; then, yeast, bacillus licheniformis and bacillus subtilis strain liquid is inoculated, high-moisture-content aerobic fermentation is carried out for 12 to 96 hours, and the fermentation temperature is 35 to 45 DEG C; then, energy feed is added, mixing and stirring are carried out, and the moisture content is regulated to 25 to 35 percent; next, lactobacillus lactis, enterococcus faecium, lactobacillus acidophilus, enterococcus faecalis and lactobacillus plantarum strain liquid is added, complex enzyme is added, the low-moisture-content anaerobic fermentation is carried out for 24 to 120 hours, and the fermentation temperature is 25 to 35 DEG C; and finally, the complete feed finished product is prepared through rolling tube drying, mixing and stirring, granulation and package. The method has the beneficial effect that the production efficiency and the feed nutrition value are improved.
Owner:江苏中煤长江生物科技有限公司
Plant microecological preparation for enhancing immunity of animal and preparation method of plant microecological preparation
InactiveCN104585493APromote growth and developmentImprove immune functionFood processingAnimal feeding stuffPediococcus speciesFeed additive
The invention discloses a plant microecological preparation for enhancing the immunity of an animal and a preparation method of the plant microecological preparation, and belongs to the field of feed additives. The plant microecological preparation is prepared by the following steps: mixing crushed materials or extracted materials of radix pseudostellariae, liquorice, rhizoma atractylodis macrocephalae, honeysuckle, astragalus membranaceus, eucommia ulmoides, great burdock achene, dandelion and herba epimedii, adding a culture solution to prepare a culture medium, adding a total bacterium liquid consisting of two or more than two of lactobacillus plantarum, enterococcus faecalis, enterococcus faecium, enterococcus faecium, pediococcus acidilactici, pediococcus pentosaceus, rhodopseudomonas palustris, bacillus subtilis and lactobacillus fermenti, fermenting and drying. The plant microecological preparation disclosed by the invention has the advantages that the immunity of the animal can be obviously improved, the production performance can be improved and the diarrhea rate can be reduced.
Owner:BEIJING KEEPYOUNG TECH
Fermented straw compound microbial agent and preparation method and application thereof
InactiveCN106497836AImprove resource utilization efficiencyImprove fertilizer efficiencyFungiBacteriaBacillus licheniformisFeces
The invention provides a fermented straw compound microbial agent and a preparation method and application thereof. The microbial agent is prepared from enterococcus faecium WEI-6, bacillus subtilis WEI-29, bacillus licheniformis WEI-30, lactobacillus plantarum WEI-64 and brewer's yeast WEI-92; the total amount of bacillus in compound bacterial liquid is 1.5*109 CFU / mL, the total amount of lactic acid bacteria is 2.5*109 CFU / mL, and the total amount of yeast is 2.07*108 CFU / mL; the compound bacterial liquid is inoculated into large-scale compost with straw and livestock excrement as the main raw materials, a certain amount of agricultural waste is added at the same time, and the excellent organic fertilizer can be obtained through fermentation maturity. The bacterial strain of the compound microbial agent is obtained from medium-temperature dominant bacterial community in a natural fermentation process of the straw compost, the bacterial strain can effectively improve an organic fertilizer fermentation production process with the straw and the livestock excrement as the main raw materials after being screened and acclimatized for a long time, and the production efficiency of the organic fertilizer and the innocent treatment effect of the straw and the excrement are improved; thus, the fermented straw compound microbial agent is suitable for livestock and poultry farms with large-scale straw resources nearby to perform innocent treatment and resource utilization of the straw and the cultivation excrement.
Owner:BEIJING DAWEIJIA BIOTECH SHARE CO LTD +1
Enterococcus faecalis capable of absorbing mycotoxin and application thereof in absorbing mycotoxin
ActiveCN101792726AImprove adsorption capacityBasic Bio-Health FunctionsBacteriaAnimal feeding stuffMycotoxinMildew
The invention discloses enterococcus faecalis capable of absorbing mycotoxin. The strain is named enterococcus faecalis SRG and is collected in China Center for Type Culture Collection on 2nd, March, 2010, and the collection number of the strain is CCTCC M 2010046. The enterococcus faecalis can absorb mycotoxin, has stronger adsorption capability and can be independently used as the mould-removing agent of a feed or can be compounded with hydrated aluminosilicate to be used as the mould-removing agent of the feed. The enterococcus faecalis has the basic biological health-care function while removing moulds. The enterococcus faecalis is compounded with the hydrated aluminosilicate with stronger mold adsorption capacity, and thereby, the mould-removing effect is achieved by physical adsorption, and the effects of mould proofing, mould removing and health care can be achieved by the bioactivity of the enterococcus faecalis.
Owner:山东宝来利来生物工程股份有限公司 +1
Gene chip of main pathogenic microorganism in drinking water and testing kit
InactiveCN101748192ALow costImprove throughputMicrobiological testing/measurementAgainst vector-borne diseasesEscherichia coliBacteroides
The invention provides a gene chip of main pathogenic microorganism in drinking water and a testing kit, which mainly aims at 11 kinds of bacteria of colibacillus / Shigella, salmonella, vibrio cholera, vibrio parahaemolyticus, staphylococcus aureus, enterococcus faecails, pseudomonas aeruginosa, legionella pneumophilia, pneumobacillus, yersinia enterocolitica and the like, and L.interrogans. The gene chip comprises a solid phase carrier and a oligonucleotide probe fixed on the solid phase carrier, wherein the oligonucleotide probe contains gyrB gene with tremendous evolutionary advantage, ITS gene and DNA segment selected from 16srRNA gene or complementary DNA segment. The gene chip and the testing kit of the invention can test the main pathogenic microorganism in drinking water, and has the characteristics of simple operation, high throughput, high accuracy, strong repeatability and the like, and can be used for clinical test for the water quality monitoring department.
Owner:NANKAI UNIV
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