62 results about "Subclinical mastitis" patented technology

The present invention includes compositions, kits and methods useful for the detection of mastitis in an animal. These agents and methods are primarily directed to a method of detecting the presence of mastitis, including sub-clinical mastitis, in cows, involving incubating a sample of milk from the cow with an agent that binds to lactoferrin such as, e.g., a monoclonal antibody specific for lactoferrin, and then detecting bound lactoferrin. The invention includes lateral-flow immunoassay methods and devices for assessing the presence of lactoferrin in milk samples.

The invention discloses a compound microorganism additive for milk cow feed. The microorganism additive consists of fermentation product mixture of natto bacillus subtilis, enterococcus faecium and saccharomyces cerevisiae, wherein the total number of bacteria is more than 10<9>cfu / g. Long-term repeating animal experiments show that three microorganisms in the compound microorganism additive can develop synergy, thereby significantly improving the milk yield of the milk cow, fat percentage in the milk and milk protein yield, significantly reducing the somatic number of the milk cow in lactation period and the disease probability of mastitis and subclinical mastitis, and significantly enhancing the autoimmunity of the milk cow organism. The compound microorganism additive has the advantages of low cost, significant effect and good application and market prospects.

The invention discloses a new traditional Chinese medicine for treating dairy cow mastitis and a preparation method thereof; the traditional Chinese medicine is a Chinese materia medica preparation prepared by using Chinese violet, dandelion, forsythia fruit and Chinese goldthread as raw materials. The traditional Chinese medicine of the invention has obvious preventive and therapeutic actions on subclinical mastitis and clinical mastitis of dairy cows, which has the advantages of simple process, low production cost, no residue, convenient use, etc.

The invention relates to the field of feed additives, in particular to a functional dairy cattle feed additive capable of preventing subclinical mastitis of dairy cattle. According to the functional dairy cattle feed additive, based on the total weight of the additive, the functional dairy cattle feed additive contains 2%-6% of organic selenium, 2%-6% of organic zinc, 4-8% of biological antioxidant, 8%-12% of mold adsorbent, 0.2%-0.6% of vitamins, 8%-12% of conventional additive for reducing the number of somatic cells, and 50%-80% of carrier. The invention provides the functional dairy cattle feed additive capable of preventing the subclinical mastitis of the dairy cattle and reducing the number of the somatic cells, the formula is reasonable, the nutrition is balanced, the number of thesomatic cells in the dairy cattle can be effectively reduced, the incidence of the subclinical mastitis of the dairy cattle can be reduced, the good development mechanism of a mammary system can be maintained, and the milk yield and the milk quality of the dairy cattle can be improved.

A system that attached to mechanized milking equipment allows its automation that solves the subclinical mastitis by “differentiating” among each individual mammary quarter the time when teat cups must be removed after the milk is over, in an asynchronous and independent manner from other teat cups. Minimize the negative consequences of over-milking as a results of not removing teat cups on time after the milk flow has stopped; reduces economic, financial and productivity problems arisen as a result of subclinical mastitis mainly caused by over-milking; thus improving the product quality and amount. Mastitis produces somatic cells in milk, the greater the amount of these cells the lower the price received by the producer. Damage to the mammary gland may be permanent resulting in the loss of the gland and may be contagious.The system includes teat cups, tubes, collector, discrete flow sensor, vacuum sensor, command control, software comprising as a part thereof the mammary quarters with physiological and anatomical characteristics thereof.

A device, system, and method employ spectrometry to interrogate udder tissue or milk to diagnose clinical and sub-clinical mastitis.

The invention provides a novel slow-release breast perfusion agent for preventing cow subclinical mastitis and a preparation method thereof. 100g of the novel slow-release breast perfusion agent comprises 1 to 5g of cephapirin benzathine, 0.5 to 8g of polyethylene glycol-400, Tween materials or propylene glycol, 0.5 to 8g of fatty alcohol-polyoxyethylene ether, cyclomethylene glyceryl ether, glyceryl triacetate or poloxamer, 0.02 to 0.05g of vitamin E, 0.05 to 0.1g of benzyl alcohol, and the balance soybean oil for injection, liquid paraffin or median-chain triglyceride. The invention also provides a preparation method of the novel slow-release breast perfusion agent. The novel slow-release breast perfusion agent is suspended colloid, can be released slowly, has low administration frequency, can prevent cow subclinical mastitis in a mild shortage stage, and does not produce residues in milk in a lactation stage. Compared with foreign like products, the suspended colloid has better stability.

The invention provides a novel slow-release breast perfusion agent for preventing cow subclinical mastitis in a milk shortage stage. 100g of the novel slow-release breast perfusion agent comprises 7-11g of cefalonium, 0.5 to 8g of polyethylene glycol-400, Tween materials or propylene glycol, 0.5 to 8g of fatty alcohol polyoxyethylene ether, cyclomethylene glyceryl ether, triacetin or poloxamer, 0.5 to 8g of aluminum stearate or silica, 0.02 to 0.05g of vitamin E, 0.05 to 0.1g of benzyl alcohol, and the balance liquid paraffin, soybean oil for injection or median chain triglyceride. The invention also provides a preparation method of the novel slow-release breast perfusion agent. The novel slow-release breast perfusion agent is suspension colloid, can be slowly released, can effectively reduce administration frequency, can effectively prevent and treat cow subclinical mastitis by being used in a milk shortage stage, and does not produce residues. Compared with the like foreign products, the suspension colloid has better stability.

Disclosed is a preparation method of a traditional Chinese medicine micro-ecological additive for prevention and treatment of subclinical mastitis of cows. The preparation method comprises performing superfine grinding on eucommia ulmoides leaves, ginkgo leaves, purple perilla, Chinese pulsatilla roots, coneflowers, humulus scandens, shrimp shells, milkvetch roots and licorice roots; adding water and boiling through the high heat; performing soft file insulation; performing cooling and filtration; collecting filtrate; adding white sugar and boiling; cooling down to the room temperature and performing lactobacillus, tea fungi and yeast inoculation to obtain the micro-ecological additive through fermentation; enabling the cows to drink the obtained micro-ecological additive in proportion. According to the traditional Chinese medicine micro-ecological additive, the functions of heat clearing and detoxification, sterilization and anti-inflammation, lactation promotion and the like are achieved, Chinese herbal medicine active materials, active small peptides, lactic acid and probiotics are rich, the amino acid is rich and balanced, the traditional antibiotic treatment method is replaced, and the harm of antibiotics to the health of the cows and antibiotic residues in the milk are reduced. According to the preparation method of the traditional Chinese medicine micro-ecological additive for the prevention and treatment of the subclinical mastitis of the cows, the use is simple and convenient, drug residues are not produced, any toxic and side effect is not produced, the viable bacteria content is high, the preparation method is suitable for industrialized production, and the market prospects are good.

The invention discloses a sow feed capable of increasing weaned piglet survival rate. The sow feed is prepared from, by weight, 50 to 60 parts of corn, 20 to 30 parts of soybean meal, 10 to 20 parts of rice bran, 10 to 20 parts of fish meal, and 5 to 10 parts of a plant additive; the plant additive is composed of, by weight, 10 to 20 parts of tea tree oil, 3 to 6 parts of clove, 5 to 10 parts of garlic, 10 to 15 parts of radix astragali, 5 to 10 parts of mint leaf, 10 to 20 parts of moringa seed, and 5 to 10 parts of radix isatidis. Adding of the plant additive containing spices, essential oil, and Chinese herbal medicines, into the sow feed is capable of increasing feed intake and daily lactation yield of sow, and promoting growth of piglets, and possesses positive influences on post partum colostrums quality and sow subclinical mastitis infection; uniformity is relatively high; and weaned piglet number is relatively large.

The invention relates to the field of medicine, and especially relates to an application of thymopentin in preparing medicine used for treating mastitis. As a result of subclinical mastitis efficacy test, infection mammary area numbers of thymopentin treatment groups, especially a high dose (4mg) group, have a reduction tendency after treatment. As a result of clinical mastitis efficacy test, through injection of thymopentin (TP-5) in breast lymph node for continuously 3 days, milk SCC of two TP-5 dose groups (10mg and 20mg) both have reduction tendencies, and cow breast swellings are substantially ameliorated in appearance. Therefore, medicines prepared with high-dose thymopentin (higher than 10mg) have significant effects against cow clinical mastitis. Therefore, the medicines prepared by using thymopentin have significant effects against subclinical mastitis and / or clinical mastitis.

Relating to relevant technologies of granules with Chinese herbal medicines as raw materials and for preventing and treating cow subclinical mastitis, the invention provides a formula and a preparation method of a traditional Chinese medicine granule for prevention and treatment of cow subclinical mastitis. According to the formula involved in the invention, the granule comprises, by weight: 10-60 parts of astragalus, 10-60 parts of angelica, 10-40 parts of motherwort, 10-40 parts of cowherb seeds, 10-60 parts of dandelion, 10-50 parts of beautiful sweetgum fruits, and 10-40 parts of licorice. The preparation method consists of: decocting the astragalus, the angelica, the motherwort, the cowherb seeds, the dandelion, and the beautiful sweetgum fruits with water twice, carrying out vacuum concentration and drying to obtain dry extractum, crushing it and passing it through a 100-mesh sieve to obtain extractum powder, adding an appropriate amount of an excipient, and performing granulation, drying as well as subpackaging, thus obtaining the finished product.

The invention discloses a compound microorganism additive for milk cow feed. The microorganism additive consists of fermentation product mixture of natto bacillus subtilis, enterococcus faecium and saccharomyces cerevisiae, wherein the total number of bacteria is more than 10<9>cfu / g. Long-term repeating animal experiments show that three microorganisms in the compound microorganism additive can develop synergy, thereby significantly improving the milk yield of the milk cow, fat percentage in the milk and milk protein yield, significantly reducing the somatic number of the milk cow in lactation period and the disease probability of mastitis and subclinical mastitis, and significantly enhancing the autoimmunity of the milk cow organism. The compound microorganism additive has the advantages of low cost, significant effect and good application and market prospects.

The invention relates to the field of the designing of animal disease detection apparatuses having a wireless communication function in the animal husbandry, and concretely relates to a cow subclinical mastitis detector and a use method thereof. A detection personnel holds the detector by hands, and places a probe in sampled milk, and a hardware circuit converts conductivity to a simulation voltage and inputs the simulation voltage to an MSP430 chip. The AD conversion of an external voltage is carried out through using internal ADC by the MSP430 chip, the obtained value is compared with a preset threshold, and the obtained comparison result is displayed on an LCD1602 liquid crystal screen. If the value exceeds a safe range, alarm information is sent to a PTR2000 wireless transceiver module A in an asynchronous serial communication mode. The module A sends a detection result to a PTR2000 wireless transceiver module B at a PC terminal in a wireless emission manner, and then information is sent to a PC machine through an RS232 structure. A PC terminal person receives the alarm information, sends an alarm message to a person in charge through a GSM network by utilizing a wireless communication alarm system in order to complete the whole process from field detection to real time notification.

The invention provides a preparation method and an application of a compound extremely fine powder Qicao breast recovering powder for preventing subclinical mastitis of dairy cattle, and solves the problems that products for curing the subclinical mastitis of dairy cattle are few, most of the products are powder produced through coarse grinding, the bioavailability is low, the effect is slow, and the like at present. The breast recovering powder comprises astragalus, motherwort and cowherb seeds. The preparation method can enable the formula to fully play efficacy, digestion and absorption are facilitated, the bioavailability of the drug is greatly improved, traditional Chinese medicine resources are saved, the efficacy is improved appropriately, the breast recovering powder is safe and reliable, has no toxic and side effects, and the application and popularization prospect is good.

The invention discloses an enzyme-linked immunoabsorbent kit for detecting serum amyloid protein A as well as a preparation method and an application of the kit and belongs to the field of analytical test. The kit comprises an ELISA plate coated by a monoclonal antibody of a cow serum amyloid protein A, a cow serum amyloid protein A antigen standard substance, a cow serum amyloid protein A monoclonal antibody, an enzyme labeled goat anti-rat antibody, a diluent, a scrubbing solution, a developing solution and a stop buffer. The content of the serum amyloid protein A in milk is measured by a double-antibody sandwiched indirect enzyme-linked immunosorbent assay method so as to diagnose bovine subclinical mastitis. The kit disclosed by the invention is easy and convenient to operate, has the characteristics of high specificity, high sensitivity and high reproducibility and provides a novel testing tool for detection of the bovine subclinical mastitis.

The invention discloses a Chinese herbal medicine formula capable of preventing and treating subclinical mastitis of a milk cow. The Chinese herbal medicine formula is prepared from raw materials in parts by weight as follows: 10-20 parts of Chinese angelica, 10-20 parts of phellodendron bark, 30-40 parts of indigowoad leaves, 25-35 parts of hairyvein agrimonia herb, 20-30 parts of divaricate saposhnikovia root, 40-50 parts of prunella vulgaris, 40-50 parts of uniflower swisscentaury root, 25-35 parts of root of nakedcaule groundsel, 25-35 parts of loofah sponge, 30-40 parts of rehmannia glutinosa, 30-40 parts of euphorbia humifusa willd, 30-40 parts of bunge corydalis herb, 50-60 parts of chrysanthemum indicum, 50-60 parts of rhizoma phragmitis, 20-30 parts of folium hibisci mutabilis and 20-30 parts of cape jasmine fruit. According to the Chinese herbal medicine formula capable of preventing and treating the subclinical mastitis of the milk cow, pure natural Chinese herbal medicine materials are adopted, the preparation is simple, medicine material resources are wide, the cost is low, and the effect is remarkable and reliable.

The invention relates to a gynostemma pellet preparation for preventing subclinical mastitis and a preparation method thereof, which belong to the field of veterinary medicine; and the preparation is prepared from gynostemma, starch, dextrin, cellulose acetate and polyethylene glycol 1500 with different weights, improves immunity by increasing the number of white blood cells, enhancing T cells and promoting the production of macrophages, prevents and cures mastitis, and has the advantages of high curing effect, no toxin, no side effect and no residue.

The invention provides a traditional Chinese medicine preparation with the functions of improving the immunity of dairy cows and preventing mastitis of dairy cows, belonging to the technical field of traditional Chinese medicine preparations. The traditional Chinese medicine preparation comprises the following raw materials in parts by weight: 150-200 parts of radix astragali, 40-80 parts of radix codonopsis, 40-80 parts of Chinese angelica, 40-80 parts of prepared rhizome of adhesive rehmannia, 30-60 parts of largehead atractylodes rhizome, 40-80 parts of dried tangerine peel, 15-45 parts of liquorice root, 15-45 parts of Szechuan lovage rhizome, 30-50 parts of all-grass of desertliving cistanche, 30-50 parts of dwarf lilyturf tuber, 30-50 parts of white paeony root, 60-150 parts of hawthorn fruit, 40-80 parts of radish seed, 60-100 parts of Chinese arborvitae twig, 5-15 parts of rhubarb, 10-30 parts of cape jasmine fruit, 40-80 parts of virgate wormwood herb and 40-80 parts of pig's toenail. The traditional Chinese medicine preparation has the beneficial effects that the traditional Chinese medicine preparation has the functions of improving the immunity of the organism and effectively reducing the morbidity of subclinical mastitis and clinical mastitis of dairy cows; the traditional Chinese medicine preparation has the functions of improving the rumen functions, protecting the digestive tract mucosa, reducing diseases of digestive tract and increasing the feed utilization rate; the traditional Chinese medicine preparation has the function of reducing the morbidity of the disease of foot and limb and alcohol positive milk of dairy cows; the traditional Chinese medicine preparation is resistant to various heat and cold stresses and has the effect of reducing production loss; and the traditional Chinese medicine preparation achieves health and high yield of dairy cows and has the effect of improving the health levels of dairy cows.

The invention discloses a reagent and a test strip for diagnosing bovine subclinical mastitis and a preparation method thereof. The reagent comprises the following components: catechol violet, neutral red, litmus, Nile blue A and alpha-naphtholbenzein. The invention further discloses a diagnostic test strip prepared from the reagent for diagnosing bovine subclinical mastitis and a usage method thereof. The diagnostic test strip provided by the invention has advantages of low cost, convenient usage and high diagnostic accuracy. Clinical diagnostic test results show that the test paper provided by the invention has an accuracy rate higher than 95% on detecting bovine subclinical mastitis, and can be applied to clinical detection of bovine subclinical mastitis.

The invention provides a method for preparing a traditional Chinese medicine preparation for improving dairy cow immunity and preventing dairy cow mastitis, and belongs to the technical field of traditional Chinese medicine preparations. The method comprises the following steps: respectively processing radix rehmanniae preparata, haws, semen raphani, rheum officinale and pig nails, then mixing the processed materials with astragalus membranaceus, codonopsis pilosula, angelica sinensis, rhizoma atractylodis macrocephalae, pericarpium citri reticulatae, licorice root, ligusticum wallichii, cistanche salsa, radix ophiopogonis, radix paeoniae alba, cacumen biotae, cape jasmine and capillary artemisia, and grinding to obtain ground material; then, adding the ground material into corn flour, glucose and water, and uniformly mixing; adding zymophyte, performing aerobic fermentation for 48-72 hours, and performing anaerobic fermentation for more than 1 week; drying in a shadow place, and adding a carrier to obtain the traditional Chinese medicine preparation for improving dairy cow immunity and preventing dairy cow mastitis. The traditional Chinese medicine preparation can be used for improving body immunity and effectively reducing the incidence rate of subclinical mastitis and clinical mastitis of dairy cows, so that health and high output of dairy cow can be realized and the healthy level of dairy cow can be improved.

The invention provides a preparation method and an application of compound traditional Chinese medicine Qicao breast recovering granules for preventing subclinical mastitis of dairy cattle, and effectively solves the problems that products for curing the subclinical mastitis of dairy cattle are few, most of the products are antibiotics, problems of drug resistance and residue of antibiotics are serious and the like at present. The breast recovering granules are prepared by astragalus, motherwort and cowherb seeds through water extraction, concentration, drying and addition of accessories. The breast recovering granules are safe and reliable, has no toxic and side effects, and the application and popularization prospect is good.

The invention relates to compound traditional Chinese medicine powder for treating dairy cow subclinical mastitis. The compound traditional Chinese medicine powder is prepared by, by weight, 8-10 parts of polygonum cuspidatum, 8-10 parts of sanguisorba officinalis, 6-8 parts of Cyrtomium fortunei, 8-10 parts of Scutellariae barbatae, 8-10 parts of taraxacum mongolicum, 8-10 parts of Chinese pulsatilla root, 8-10 parts of fructus crataegi, 8-10 parts of radix bupleuri, 8-10 parts of Agrimonia pilosa, 8-10 parts of Loropetalum chinense leaf, 8-10 parts of Polygonum perfoliatum, 8-10 parts of herba houttuyniae, 8-10 parts of Acalypha australis, 8-10 parts of phyllanthus ruinaria, 8-10 parts of Euphorbia humifusa, 2-4 parts of herba leonuri and 4-6 parts of semen vaccariae. The compound traditional Chinese medicine powder for treating the dairy cow subclinical mastitis is high in treatment rate, good in inflammation resistance, safe, free of toxic and side effects, and the like.

The invention discloses a Chinese herbal preparation for preventing and treating dairy cow subclinical mastitis and reducing milk SCC. The Chinese herbal preparation is composed of, by weight part, 6parts of herba taraxaci, 6 parts of herba violae, 4 parts of fructus forsythia, 3 parts of flos lonicerae, 2 parts of rhizoma coptidis, 2 parts of radix paeoniae rubra, 3 parts of radix codonopsis, 3parts of radix angelicae sinensis, 3 parts of fructus trichosanthis and 2 parts of radix glycyrrhizae. The Chinese herbal preparation for preventing and treating dairy cow subclinical mastitis and reducing milk SCC is reasonable in design, and through research on compound traditional Chinese medicines for preventing and treating dairy cow subclinical mastitis and reducing milk SCC, improves the preventing and treating effects of Chinese herbs on dairy cow subclinical mastitis and provides a theoretical basis for comprehensive prevention and treatment on dairy cow subclinical mastitis and reduction of milk SCC with Chinese herbs.

The invention relates to a long-acting breast injection agent for treating cow mastitis in dry period and a preparation method of the long-acting breast injection agent. The long-acting breast injection agent is prepared from the following raw materials: cefalonium, a tanshinone extract, a slow-release agent, an emulsifier, an antioxidant and an oily solvent. The breast injection agent provided by the invention is mainly used for preventing and treating clinical mastitis and subclinical mastitis caused by staphylococcus aureus, streptococcus, Escherichia coli and the like, has the characteristics of long-lasting property, high stability and high efficiency, and has good clinical application value and market prospects.

The invention relates to a diagnostic reagent for detecting subclinical mastitis of mammals such as cows, sheep, pigs and the like and a preparation method for the reagent. The detection reagent is formed by the following components by mass percent: 8.8-9.2 percent of lauryl sodium sulfate, 4.8-5.2 percent of linear alkyl benzene sulfonic acid, 0.5-0.6 percent of boric acid, 0.04-0.08 percent of bromcresol purple, 0.6-0.8 percent of sodium hydroxide and remaining percent of water. The preparation method is that lauryl sodium sulfate, linear alkyl benzene sulfonic acid and boric acid are mixed first, a little of water (proper amount) is added for agitation to enable lauryl sodium sulfate, linear alkyl benzene sulfonic acid and boric acid to be fully dissolved, then bromcresol purple aqueous solution is added and is fully and evenly agitated, and finally sodium hydroxide aqueous solution and water are added in the obtained solution.

A pure platelet-rich plasma (P-PRP) composition as an alternative to conventional antibiotic treatment of subclinical mastitis caused by Gram-positive bacteria in bovine including five live platelets and leukocytes, an anticoagulant, and an activating substance.

The invention relates to a long-acting cloxacillin benzathine breast injectant. The long-acting cloxacillin benzathine breast injectant is prepared from the following raw materials in parts by weight: 3-20 parts of cloxacillin benzathine, 30-90 parts of ethyl oleate, 10-30 parts of beewax and 1-3 parts of tween-80. The invention also provides a preparation method of the long-acting cloxacillin benzathine breast injectant. The long-acting cloxacillin benzathine breast injectant has the advantages that the inclusion rate of active ingredients of the long-acting cloxacillin benzathine breast injectant is high, a cellular structure of beewax is small, a good sustained release effect can be realized, effective minimal inhibitory concentration can be prolonged to 58 days, breast tissues are more elastic after the long-acting cloxacillin benzathine breast injectant is applied, scar tissues are stable and non-infiltration, functions of smooth muscles of nipples can be effectively restored, and treatment effect on subclinical mastitis is good compared with that of the prior art. The preparation method of the long-acting cloxacillin benzathine breast injectant has the advantages that active ingredients of the beewax are effectively reserved and are processed into microcapsules, so that the contact area between the beewax and the breast tissues is increased, and the medical effect of the beewax can be fully played.