The invention relates to an influenza virus and 2019 novel coronavirus antibody combined detection device and a preparation method thereof and belongs to the field of medical detection equipment. Thekit is prepared by adhering a nitrocellulose film, a glass fiber film, a sample pad, absorbent paper and other auxiliary materials, wherein the nitrocellulose film contains an influenza virus A antigen, an influenza virus B antigen, a novel coronavirus S antigen and a goat-anti-mouse IgG polyclonal antibody in a solid phase, and the glass fiber film absorbs a high-specificity anti-human IgM antibody. On the basis of ensuring complete release of the immune colloidal gold, the reaction sensitivity is effectively improved, under the same threshold value, the dosage of the immune colloidal gold can be reduced, cost is saved, the influenza virus A, the influenza virus B and novel coronavirus IgM antibodies in a specimen can be detected at the same time, and complexity of production operation isnot increased. The test paper is high in sensitivity, strong in specificity, simple and convenient to operate, time-saving and strong in practicability.