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Protein chip for detecting respiratory virus antigens as well as kit and preparation method thereof

A technology of protein chips and virus antigens, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of PCR method pollution, high hardware requirements, and inability to detect 8 kinds of viruses at the same time, and achieve the effect of saving time

Inactive Publication Date: 2016-04-20
深圳市赛尔生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many such products on the market at present, and the PCR method is easy to cause pollution. The requirements for the user unit and laboratory hardware are relatively high, and it is only used under scientific research conditions.
[0008] To sum up, the above methods have their own advantages and disadvantages, but they all need to detect only one virus or one antibody in one experiment, and cannot detect the above 8 viruses at the same time
It is time-consuming and labor-intensive in terms of detection efficiency, and it takes 3-5 days for isolation and culture
Although the PCR method is sensitive and specific, it takes several hours and is not commonly used. It also requires expensive instruments. One experiment can only diagnose one virus, which cannot be implemented in primary medical institutions because it requires a strictly controlled operating space and clean environment

Method used

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  • Protein chip for detecting respiratory virus antigens as well as kit and preparation method thereof
  • Protein chip for detecting respiratory virus antigens as well as kit and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A protein chip for detecting respiratory virus antigens, comprising an aldehyde-treated glass slide and a quality control protein array fixed in quality control control holes on the slide; the quality control proteins include adenovirus antigens, respiratory tract Syncytial virus antigen, influenza A, B antigen, parainfluenza virus antigen, SARS virus antigen, H5N1 antigen and H1N1-A antigen.

[0048] Prepared by the following method:

[0049] (1) Take a glass slide of standard size, soak it overnight in a mixture of concentrated sulfuric acid and potassium permanganate, wash it with water and then soak it in ammonia water (ammonia: water = 1:3 volume ratio) overnight; wash it with water and then soak it in concentrated hydrochloric acid (concentrated HCI: H 2 O=1:3 volume ratio); after taking it out, wash it with water and dry it.

[0050] (2) Soak the dried glass slide in ammoniated solution for ammoniation for 1 hour, take it out, wash it and dry it; then soak it i...

Embodiment 2

[0060] A protein chip and kit for detecting respiratory virus antigens, including the protein chip described in Example 1, and other supporting reagents, the other supporting reagents are:

[0061] (1) Biotin-labeled specific antibody, diluted with a specific diluent, packed in a reagent bottle and sealed. The biotin-labeled specific antibodies respectively contain anti-adenovirus (ADV), anti-respiratory syncytial virus (RSV), anti-influenza type A (IFV-A), anti-type B virus (IFV-B), anti-parainfluenza virus (PIV), anti-SARS virus, anti-avian influenza virus (H5N1) and anti-influenza A virus (H1N1);

[0062] (2) Streptavidin-labeled alkaline phosphatase (AP) or horseradish peroxidase (HRP).

[0063] (3) Specific substrate for streptavidin-labeled alkaline phosphatase (AP) or horseradish peroxidase (HRP).

[0064] Assemble the above reagents and the protein chip of Example 1 to form a protein chip kit for detecting 8 kinds of respiratory virus antigens.

[0065] Using this p...

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Abstract

The invention provides a protein chip for detecting respiratory virus antigens as well as a kit and a preparation method thereof. The protein chip for detecting the respiratory virus antigens comprises a glass matrix vector and a quality control protein probe fixed in a protein quality comparison hole in the glass matrix vector; quality control protein comprises an adenovirus antigen, a respiratory syncytial virus antigen, influenza A and B antigens, a parainfluenza virus antigen, a SARS virus antigen, an H5N1 antigen and an H1N1-a antigen. Through adoption of the technical scheme, the diagnosis of simultaneous detection of eight types or more types of viral infection can be realized through one experiment, so that the efficiency in diagnosis of respiratory viral infectious diseases is improved, anti-bioterrorism screening is assisted, and a purpose of efficient, time-saving and high-flux diagnosis is realized.

Description

technical field [0001] The invention belongs to the technical field of biochips, and in particular relates to a protein chip, a kit and a preparation method for detecting respiratory virus antigens. Background technique [0002] Respiratory viruses are the pathogens of human respiratory tract infection and are one of the most common infectious diseases. According to the World Health Organization, infectious diseases account for the highest number of deaths in the world today, especially in most developing countries. The 21st century has returned to the era of fighting infectious diseases. The most common respiratory viruses infecting humans are adenovirus (ADV), respiratory syncytial virus (RSV), influenza virus (IFV-A, IFV-B) and parainfluenza virus (PIV), which are common pathogens of upper respiratory tract infections. The infected parts are all in the same part, and the infection is repeated, the symptoms are very similar, and it is difficult to distinguish. The rest,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/552
CPCG01N33/56983G01N33/552G01N33/6803G01N2333/11G01N2333/115G01N2333/135
Inventor 林连成彭杰雄李琼英刘富菊上官美荣
Owner 深圳市赛尔生物技术有限公司
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