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Influenza virus and 2019 novel coronavirus antibody combined detection device and preparation method thereof

A technology for influenza virus and coronavirus, which is applied in the joint detection device for influenza virus and 2019 novel coronavirus antibody and its preparation field, can solve the problems of insufficient amount of adsorbed protein on NC membrane and weak binding force, etc., and achieve a reasonable and comprehensive judgment, Easy operation and strong practical effect

Active Publication Date: 2020-08-25
杨小军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The invention provides a joint detection device for influenza virus and 2019 novel coronavirus antibody and its preparation method to solve the problems of insufficient NC membrane adsorption protein and weak binding force existing in the prior art

Method used

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  • Influenza virus and 2019 novel coronavirus antibody combined detection device and preparation method thereof
  • Influenza virus and 2019 novel coronavirus antibody combined detection device and preparation method thereof
  • Influenza virus and 2019 novel coronavirus antibody combined detection device and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] The sample pad 1, the immune colloidal gold glass fiber membrane 2, the immune nitrocellulose membrane 3, and the absorption pad 4 are pasted on the plastic plate 5 respectively, and the two ends of the immune nitrocellulose membrane 3 are respectively connected to the absorption pad 4, the immune colloidal gold glass The fiber membrane 2 is overlapped, and the other end of the immune colloidal gold glass fiber membrane 2 is overlapped with the sample pad 1; the immune nitrocellulose membrane 3 is provided with a first detection line T1, a second detection line T2, and a third detection line. Line T3, and quality control line C; said first detection line T1 has influenza virus A antigen on the solid phase; said second detection line T2 has influenza virus B antigen on the solid phase; said third detection line The solid phase on T3 has a highly specific 2019 novel coronavirus S antigen; the detection lines T1, T2, and T3 set on the immune nitrocellulose membrane 3 can be...

Embodiment 2

[0069] Colloidal gold particles are 40nm;

[0070] Gold-spraying buffer solution includes: the concentration is 20mM Tris-HCL solution, the sucrose concentration is 12%, the trehalose concentration is 3%, the BSA concentration is 0.7%,

[0071] Preparation of polyethylene glycol glycerin treatment solution: mixed with polyethylene glycol glycerin and polylysine (SIGMA, 150KD), wherein the concentration of polyethylene glycol glycerin is 0.5%, and polylysine The concentration is 0.5%, filtered through a 0.22μm filter membrane, and set aside;

[0072] The concentration of Tris-HCL solution is 0.1M, the concentration of bovine serum albumin BSA is 0.7%, the concentration of casein is 0.15%, the concentration of surfactant is 0.7%, and it is dried at 37°C for later use. After the treatment, the sample pad can improve the reaction sensitivity;

[0073] All the other are with embodiment 1.

Embodiment 3

[0075] Colloidal gold particles are 60nm;

[0076] Gold-spraying buffer solution includes: the concentration is 20mM Tris-HCL solution, the concentration of sucrose is 20%, the concentration of trehalose is 5%, the concentration of BSA is 1%,

[0077] Preparation of polyethylene glycol glycerin treatment solution: mixed with polyethylene glycol glycerin, polylysine (SIGMA, 150KD) and PEG2000, wherein the concentration of polyethylene glycol glycerin is 0.5%, poly Lysine concentration is 0.5%,

[0078] The concentration of PEG20000 is 0.1%, filtered through a 0.22 μm filter membrane, and set aside;

[0079] The concentration of Tris-HCL solution is 0.1M, the concentration of bovine serum albumin BSA is 1%, the concentration of casein is 0.2%, the concentration of surfactant is 1%, and it is dried at 37°C for later use. After the treatment, the sample pad can improve the reaction sensitivity;

[0080] All the other are with embodiment 1.

[0081] Further illustrate the effect...

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Abstract

The invention relates to an influenza virus and 2019 novel coronavirus antibody combined detection device and a preparation method thereof and belongs to the field of medical detection equipment. Thekit is prepared by adhering a nitrocellulose film, a glass fiber film, a sample pad, absorbent paper and other auxiliary materials, wherein the nitrocellulose film contains an influenza virus A antigen, an influenza virus B antigen, a novel coronavirus S antigen and a goat-anti-mouse IgG polyclonal antibody in a solid phase, and the glass fiber film absorbs a high-specificity anti-human IgM antibody. On the basis of ensuring complete release of the immune colloidal gold, the reaction sensitivity is effectively improved, under the same threshold value, the dosage of the immune colloidal gold can be reduced, cost is saved, the influenza virus A, the influenza virus B and novel coronavirus IgM antibodies in a specimen can be detected at the same time, and complexity of production operation isnot increased. The test paper is high in sensitivity, strong in specificity, simple and convenient to operate, time-saving and strong in practicability.

Description

technical field [0001] The invention relates to the field of medical testing equipment, in particular to a joint detection device for 2019-nCoV, influenza virus type A and type B IgM antibodies and a preparation method thereof, which uses colloidal gold immunochromatography technology and the principle of capture method to quantitatively detect whole blood and serum , A detection device for human influenza virus type A, influenza virus type B, and 2019 novel coronavirus IgM antibody in plasma samples and a preparation method thereof, which can realize sensitive, specific, and rapid detection of respiratory infectious diseases. Background technique [0002] Influenza is an acute respiratory infectious disease caused by influenza virus infection. It spreads rapidly through the respiratory tract with a short incubation period and obvious seasonality. It is very easy to cause large-scale outbreaks and is difficult to control. Influenza viruses are divided into three types: A (A)...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/58G01N33/569G01N33/558G01N33/532G01N33/531
CPCG01N33/531G01N33/532G01N33/558G01N33/56983G01N33/587G01N33/6854G01N2333/11G01N2333/165G01N2469/20
Inventor 杨小军李欣
Owner 杨小军
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