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Stomach function and stomach cancer risk detection device and preparation method thereof

A technology of risk detection and function, applied in measurement devices, biological tests, material inspection products, etc., can solve the problems of weak binding force and insufficient amount of protein adsorbed by NC membrane, and achieves reduction of dosage, accurate disease risk judgment, and novel concept. Effect

Active Publication Date: 2020-09-08
JILIN PROVINCE GERUISITE BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The present invention provides a gastric function and gastric cancer risk detection device and its preparation method to solve the problems of insufficient NC membrane adsorption protein quantity and weak binding force existing in the prior art strong question

Method used

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  • Stomach function and stomach cancer risk detection device and preparation method thereof
  • Stomach function and stomach cancer risk detection device and preparation method thereof
  • Stomach function and stomach cancer risk detection device and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Including sample pad 1, immune colloidal gold glass fiber membrane 2, immune nitrocellulose membrane 3, absorbent pad 4, plastic plate 5, wherein, the sample pad 1, immune colloidal gold glass fiber membrane 2, immune nitrocellulose membrane 3 , absorption pad 4 is pasted on plastic plate 5 respectively, and the two ends of described nitrocellulose membrane 3 overlap with absorption pad 4, immune colloidal gold glass fiber membrane 2 respectively, and the other end of described immune colloidal gold glass fiber membrane 2 is connected with The sample pad 1 is overlapped; the first detection line (T1), the second detection line (T2), the third detection line (T3), the third detection line (T4), and the first detection line are set on the nitrocellulose membrane (3). Three detection lines (T5), quality control lines (C1) and quality control lines (C2); the solid phase of the first detection line (T1) has a highly specific gastrin 17 antibody; the second detection The soli...

Embodiment 2

[0069] The gold-spraying buffer includes: a concentration of 20mM Tris-HCL solution, a sucrose concentration of 12%, a trehalose concentration of 3%, a BSA concentration of 0.7%, and a pH of 8.5;

[0070] Preparation of polyethylene glycol glycerin treatment solution: mixed with polyethylene glycol glycerin and polylysine (SIGMA, 150KD), wherein the concentration of polyethylene glycol glycerin is 0.5%, and polylysine The concentration is 0.5%, filtered through a 0.22μm filter membrane, and set aside;

[0071] The concentration of Tris-HCL solution is 0.1mol / L, the concentration of bovine serum albumin BSA is 0.7%, the concentration of casein is 0.15%, and the concentration of surfactant is 0.7%;

[0072] All the other are with embodiment 1.

Embodiment 3

[0074] The gold-spraying buffer solution includes: a concentration of 20mM Tris-HCL solution, a sucrose concentration of 20%, a trehalose concentration of 5%, a BSA concentration of 1%, and a pH of 8.5;

[0075] Preparation of polyethylene glycol glycerin treatment solution: mixed with polyethylene glycol glycerin, polylysine (SIGMA, 150KD) and PEG2000, wherein the concentration of polyethylene glycol glycerin is 0.5%, poly The concentration of lysine is 0.5%, the concentration of PEG20000 is 0.1%, and it is filtered through a 0.22 μm filter membrane, and it is set aside;

[0076] The concentration of Tris-HCL solution is 0.1mol / L, the concentration of bovine serum albumin BSA is 1%, the concentration of casein is 0.2%, and the concentration of surfactant is 1%;

[0077] All the other are with embodiment 1.

[0078] Further illustrate the effect of the present invention by experiment below.

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Abstract

The invention relates to a stomach function and stomach cancer risk detection device and a preparation method thereof, and belongs to the field of medical detection equipment. The device is prepared by adhering a nitrocellulose membrane with a solid phase containing high-specificity gastrin 17, pepsinogen I, pepsinogen II antibody, anti-human IgG antibody, anti-human IgM antibody and goat-anti-mouse IgG polyclonal antibody, a glass fiber membrane adsorbed with colloidal gold labeled G-17, PGI and PGII antibodies and HP urease antigens, a sample pad, absorbent paper and other auxiliary materials. According to the invention, on the basis of ensuring complete release of immune colloidal gold, the reaction sensitivity is effectively improved; under the same threshold value, the dosage of immune colloidal gold can be reduced, the cost is saved, five gastric function evaluation and gastric cancer risk markers of G-17, PGI, PGII, HP urease IgG antibodies and IgM antibodies in a specimen can be detected at the same time, and the complexity of production operation is not increased. The test paper is high in sensitivity, strong in specificity, simple and convenient to operate, time-saving and strong in practicability.

Description

technical field [0001] The invention relates to the field of medical detection equipment, in particular to a combined detection device for G-17, PGI, PGII, HP urease IgG and IgM antibodies and a preparation method thereof, which utilizes colloidal gold immunochromatography technology and the principle of double-antibody sandwich method for quantitative detection Human gastrin 17 (G-17), pepsinogen I (PGI), pepsinogen II (PGII), Helicobacter pylori (HP) urease IgG and Helicobacter pylori urease in whole blood, serum, plasma specimens The detection device for IgM and the preparation method thereof can realize gastric function assessment, gastric cancer risk assessment, and sensitive, specific and rapid detection of various markers. Background technique [0002] Gastric mucosal lesions are caused by a variety of factors, including drugs, alcohol, abnormal gastric acid secretion, and Helicobacter pylori infection, among which Helicobacter pylori infection is the main factor; aft...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74G01N33/573G01N33/558G01N33/58G01N33/574G01N33/543
CPCG01N33/74G01N33/573G01N33/558G01N33/587G01N33/57484G01N33/57446G01N33/54346G01N2333/595G01N2333/96477G01N2333/98G01N2800/60G01N2800/06
Inventor 杨小军李欣
Owner JILIN PROVINCE GERUISITE BIOTECHNOLOGY CO LTD
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