57 results about "Pepsinogen II" patented technology

The invention discloses a test paper strip using colloidal gold immunochromatographic technology for quantitative determination of serum pepsinogen and a preparation method and application thereof. A kit comprises the test paper strip respectively used for detection of pepsinogen I and pepsinogen II, the test paper strip orderly comprises a sample pad, a gold labeled pad, a cellulose nitrate film and a water absorbent pad according to the order of connection, and also includes a plastic bottom lining located below, the role of the plastic bottom lining is to provide a platform for assembling, the cellulose nitrate film is provided with a pepsinogen I or pepsinogen II spraying quality control line, and a pepsinogen I or pepsinogen II second antibody spraying test line, the gold labeled pad is coated with a colloidal gold labeled pepsinogen I or pepsinogen II monoclonal antibody, and the sample pad provides a to-be-tested sample adding position. The kit can be used for direct quantitative detection of the pepsinogen without need of professional training, is convenient and fast in operation, and suitable for promotion and application in primary hospitals and medical centers.

The invention relates to a kit for measurement of the contents of pepsinogen I (PGI) and pepsinogen II (PGII) in serum. To overcome a technical problem, the invention provides the PGI and PGII content measurement kit applicable to a nephelometric instrument or fully automatic biochemical analyzer. The kit comprises the following components: a, R1, which is composed of a buffer solution, an accelerator and a surfactant, with the balance being purified water; b, R2-I, which is composed of a buffer solution and a latex microsphere binding with an antibody against human PGI; c, R2-II, which is composed of a buffer solution and a latex microsphere binding with an antibody against human PGII; d, a calibrator 1, which is composed of a buffer solution, a stabilizing agent, an antiseptic and a certain amount of pure recombinant PGI protein, with the balance being purified water; and e, a calibrator 2, which is composed of a buffer solution, a stabilizing agent, an antiseptic and a certain amount of pure recombinant PGII protein, with the balance being purified water Through combination of the above-mentioned reagents, the kit can rapidly measure the contents of PGI and PGII in serum.

The invention discloses a pepsinogen II detection kit, which comprises a reagent R1 and a reagent R2, wherein the reagent R1 is prepared from 50-150 mmol / L of sodium phosphate buffer solution, 2-3 g / Lof bovine serum albumin, methylisothiazolinone with the concentration of 2-3%, 1.0-5.0 g / L of fatty alcohol and ethylene oxide condensate, 3-5 mmol / L of magnesium chloride and 20-30 mmol / L of ammonium formate, and the balance of deionized water.; and the reagent R2 is prepared from 1.3-2 mg / ml of pepsinogen II antibody emulsion solution, 2-3 g / L of bovine serum albumin, methylisothiazolinone withthe concentration of 2-3%, and the balance of deionized water. The prepared pepsinogen II detection kit and the preparation method of the pepsinogen II detection kit have the advantages that the chyle problem can be effectively solved, the anti-interference capability is high, and meanwhile, the stability and the sensitivity are high.

The invention relates to a recombinant antibody against human pepsinogen II (PG II), and studies preparation and application of the recombinant antibody. The binding protein has strong activity, has high affinity with human PG II protein, and can be widely used in the field of detection of the PG II protein.

The invention relates to a time resolution fluorescence immunochromatographic test strip and test card for simultaneously and quantitatively detecting contents of pepsinogen I and pepsinogen II as well as an application of the test strip. According to the test strip disclosed by the invention, a nitrocellulose membrane is provided with a first detection zone, a second detection zone and a quality control zone which are sprayed at intervals, the first detection zone is coated with a pepsinogen I monoclonal antibody I, the second detection zone is coated with a pepsinogen II monoclonal antibody II, the quality control zone is coated with a rabbit anti-mouse polyclonal antibody, and a bonding pad is coated with a macromolecular nano microspherical coating with the surface decorated by the pepsinogen I monoclonal antibody I and the pepsinogen I monoclonal antibody and containing rare-earth ions; the test strip can simultaneously detect the contents of the pepsinogen I and the pepsinogen II, the problems that a pepsinogen test box cannot simultaneously detect the pepsinogen I and the pepsinogen II in the prior art can be solved, the high accuracy and the small error in the detection result of the pepsinogen I and the pepsinogen II in same sample liquid can be guaranteed, and the great convenience can be provided for the clinical use.

The invention relates to a fluorescent microsphere detection device for gastric function and gastric cancer risk and a preparation method thereof, and belongs to the field of medical detection equipment. The device is prepared by adhering a nitrocellulose membrane with solid phase containing high-specificity gastrin 17, pepsinogen I, pepsinogen II antibody, anti-human IgG antibody, anti-human IgMantibody and goat-anti-mouse IgG polyclonal antibody, glass fibers adsorbed with fluorescent microsphere labeled gastrin 17, pepsinogen I, pepsinogen II antibodies and helicobacter pylori urease antigens, a sample pad, absorbent paper and other auxiliary materials. The reaction sensitivity is effectively improved; under the same threshold value, the dosage of the immunofluorescence microspheres can be reduced, the cost is saved; five gastric cancer risk markers including gastrin 17, pepsinogen I, pepsinogen II, helicobacter pylori urease IgG antibody and helicobacter pylori urease IgM antibodyin a specimen can be detected at the same time, and the complexity of production operation is not increased. The detection test paper is high in sensitivity, strong in specificity and strong in practicability.

The invention discloses a kit for determining pepsinogen II and a preparation method thereof. The kit comprises double liquid components including a reagent R1 and a reagent R2 which are independent from each other, wherein the reagent R1 is prepared from a buffering solution, inorganic salt, an accelerant and a preservative; and the reagent R2 is prepared from a buffering solution, a stabilizer, a preservative and an emulsion-coated anti-human pepsinogen II antibody. The preparation method comprises the following steps: preparing the reagents according to the content of the components; mixing a sample to be detected with the reagent R1 and the reagent R2 to achieve sufficient reaction; determining a reacted absorbance difference value by utilizing a full-automatic biochemical analyzer; and calculating the concentration of the pepsinogen A in a sample according to an absorbance change value. The kit gas has the advantages of high detection accuracy and the like.

The invention relates to a stomach disease detection kit, and belongs to the technical field of in-vitro reagent diagnosis. The stomach disease detection kit is characterized in that a nucleic acid aptamer which can be specifically combined with pepsinogen II is screened by a SELEX (systematic evolution of ligands by exponential enrichment) technique, the pepsinogen II in blood is specifically screened or detected by the nucleic acid aptamer, and the nucleic acid aptamer is prepared into the detection kit, so that the direct reaction for detection without thinning of blood is realized, and the workload is decreased; the measuring range is wide, and the stomach disease detection kit is suitable for clinical application. The stomach disease detection kit has the beneficial effects that the sensitivity is high, the specificity is good, the measuring range is wide, the operation is simple, the stomach disease detection kit is suitable for large-scale popularization and application, and the market prospect is broad.