1937results about How to "High fluorescence intensity" patented technology

High-molecular compounds comprising repeating units represented by the general formula (1) or (2) and having number-average molecular weights of 10³ to 10⁸ in terms of polystyrene: (1) [wherein Ar¹ and Ar² are each independently a trivalent aromatic hydrocarbon group or a trivalent heterocyclic group; and X¹ and X² are each independently O, S, C(═O), S(═O), SO₂, C(R¹)(R²), Si(R³)(R⁴), N(R⁵), B(R⁶), P(R⁷), or P(═O)(R⁸), with the provisos that X¹ and X² must not be the same and that X¹ and Ar² are bonded respectively to the adjacent carbon atoms constituting the aromatic ring of Ar¹, and X² and Ar¹ are bonded respectively to the adjacent carbon atoms constituting the aromatic ring of Ar²] (2) [wherein Ar³ and Ar⁴ are each independently a trivalent aromatic hydrocarbon group or a trivalent heterocyclic group; and X³ and X⁴ are each independently N, B, P, C(R⁹), or Si(R¹⁰), with the provisos that X³ and X⁴ must not be the same and that X³ and Ar⁴ are bonded respectively to the adjacent carbon atoms constituting the aromatic ring of Ar³, and X⁴ and Ar³ are bonded respectively to the adjacent carbon atoms constituting the aromatic ring of Ar⁴].

The present invention relates to a detection method of quantum point labeled quick immunochromatographic test paper strip, belonging to the field of detection technology. Said invention is characterized by that the antibody of quantum point labeled objective material can be coated on the glass fibre membrane, another antibody of objective material and second antibody are respectively coated on the nitrocellulose membrane or nitrocellulose/acetyl cellulose mixed membrane to form detection band and quality control band, on the polyester or plastic plate the glass fibre membrane and nitrocellulose membrane can be made into the immunochromatographic test paper strip. Said invention also provides the concrete steps of said detection method by utilizing said test paper strip and the concrete application range of said test paper strip.

Disclosed are a nanoparticle-doped porous bead with a highly enhanced photoluminescence without wavelength shift and improved durability, and a fabrication method thereof, the nanoparticle-doped porous bead comprising porous beads, and nanoparticles radially bonded onto homocentric spheres of the porous beads by an electrostatic attractive force, the homocentric sphere located inside the porous bead near a surface thereof, wherein the nanoparticles are photoluminescent nanoparticles or mixed nanoparticles of photoluminescent nanoparticles and another nanoparticles, wherein the another nanoparticle is one or more than two mixed, selected from a group consisting of magnetic nanoparticle, metallic nanoparticle and metal oxide nanoparticle.

The invention provides a method for detecting by using quantum dot fluorescence immunochromatographic test strips. The method comprises the following specific steps: (1) preparing a quantum dot labeled protein liquid; (2) preparing a quantum dot labeled protein detecting liquid; (3) preparing a reaction film coated with a detection index; (4) bonding a sample pad, the reaction film prepared in the step (3) and a water absorbing pad on a PVC backing in sequence so as to obtain a test paper board; cutting the test paper board into test strips; and loading the test strips into a test paper clip; and (5) qualitatively or quantitatively detecting. The method for detecting by using the quantum dot fluorescence immunochromatographic test strips, provided by the invention, is a rapid detecting method using the quantum dot fluorescence immunochromatographic test strips, which is featured by high sensitivity, synchronicity in multi-index detection, simplicity, convenience, intuitive nature, low price and wide application.

A polymer compound comprising a repeating unit of the following formula (1) and which is useful as a light emitting material or charge transporting material and excellent in heat resistance, fluorescent intensity and the like:

(wherein, ring A and ring B represent each independently an aromatic hydrocarbon ring optionally having a substituent, at least one of ring A and ring B is an aromatic hydrocarbon ring composed of a plurality of condensed benzene rings, R_w and R_x represent each independently a hydrogen atom, alkyl group, alkoxy group or the like, and R_w and R_x may mutually bond to form a ring).

The present invention refers to nanoparticles having optically fluorescent activity. In more detail, the invention refers to a nanoparticle matrix comprising a co-aggregate of at least one charged polyelectrolyte and at least one oppositely charged active agent, wherein the active agent is a hydrophilic optically fluorescent agent, and the invention further refers to a nanoparticle comprising said nanoparticle matrix. Optionally, the nanoparticle is surface modified. The invention also refers to a method for preparing said nanoparticle, and to a method of surface modification. Furthermore, the invention refers to uses of said nanoparticle in vitro and in vivo, and to methods for in vitro and in vivo diagnosis.

The invention discloses a nucleic acid analyzing method based on cascade HCR (hybridization chain reaction), and belongs to the field of molecule detection. The nucleic acid analyzing method is characterized in that on the basis of single HCR, two stages of HCR reaction are designed, and the signals can be further amplified on the basis of single HCR; the hairpin nucleic acid probes marked with multiple different fluorescent dyes can be alternatively hybridized by a final target product, so as to form a branched DNA (deoxyribonucleic acid) nanometer structure; the signal output is provided bythe fluorescent resonance energy transfer, and the concentration of the target nucleic acid can be judged according to the change value of the fluorescence intensity of fluorophore. The nucleic acid analyzing method has the advantages that the nucleic acid analyzing method can be used for in-vitro detection of DNA and miRNA (miniature ribonucleic acid), the specificity is good, and the sensitivityis high; the nucleic acid analyzing method can be used for the imaging analysis of miRNA in cells, and be favorable for the early diagnosis on cancers.

The present invention relates generally to fluorescent proteins and fluorescent protein variants, and more specifically to monomeric and dimeric forms of Anthozoan fluorescent proteins. In one aspect, the present invention provides variants of fluorescent proteins, where the variants have a reduced propensity to tetramerize, and form dimeric or monomeric structures. In a further aspect, the present invention provides variants of fluorescent proteins, the variants being characterized by more efficient maturation than corresponding fluorescent proteins from which they are derived. The invention also relates to methods of making and using such fluorescent proteins and fluorescent protein variants, including fluorescent protein monomers and dimers.

The invention relates to an alkali halide-doped perovskite light-emitting diode. The alkali halide-doped perovskite light-emitting diode comprises a substrate, a hole transmission layer, an active light-emitting layer, an electron transmission layer, an electrode modification layer and an electrode, wherein the thickness of the active light-emitting layer is 5-100 nanometers, the active light-emitting layer comprises perovskite and an alkali halide doped in the perovskite, the molecular formula of the perovskite is one or more of CsPbCl<x>Br<3-x>, CsPbBr<x>I<3-x>, MAPbCl<x>Br<3-x>, MAPbBr<x<I<3-x>, FAPbCl<x>Br<3-x> and FAPbBr<x>I<3-x>, x is equal to 0, 1, 2 or 3, and the alkali halide is one or more of LiCl, NaCl, KCl, RbCl, LiBr, NaBr, KBr, RbBr, LiI, NaI, KI and RbI. The invention also provides a fabrication method of the alkali halide-doped perovskite light-emitting diode. The fabrication method comprises the steps of forming the hole transmission layer or the electron transmissionlayer o the substrate; modifying an alkali halide-containing perovskite precursor solution used as the active light-emitting layer on the hole transmission layer of the electron transmission layer; sequentially forming the electron transmission layer, a negative electrode modification layer and a negative electrode on the active light-emitting layer or sequentially forming the hole transmission layer, a positive electrode modification layer and a positive electrode on the active light-emitting layer; and performing package.

There is a kind of nano particle and micro-sphere biological fluorescence probem and the manufacturing method. The method mainly includes: 1) hydrosulphenyl compound with amidocyanogen is used as stabilizing agent in particle synthesis, water-soluble fluorescence nano particle whose surface is bedecked with amidocyanogen is directly prepared under mild condition, then it takes advantages of the amidocyanogen on the surface of particle to couple with biological system directly, further nano-particle biological fluorescence probe is acquired; 2) said fluorescence nano particle whose surface is bedecked with radical dumpling with different functions is compounded into micro-sphere is inorganic silicon dioxide or organic polymer by various means, fluorescence micro-sphere which can be dispersed equably in aqueous solution is acquired.

The invention relates to a copper ion/mercury ion fluorescence molecular probe, and a preparation method and application thereof. The invention mainly aims to solve the problem of single detection ion in the fluorescence molecular probe for detecting copper ions or mercury ions in the prior art, and the problems of low sensitivity, poor selectivity, high sensitivity to pH value, and the like in most fluorescence molecular probes. The preparation method of the fluorescence molecular probe comprises the following steps: (1) dissolving 1 part of rhodamine B in ethanol, adding 2-5 parts of hydrazine hydrate at room temperature, stirring under reflux for 1-2 hours, cooling, and filtering to obtain a precipitate; and (2) adding 1 part of precipitate into 50-500 parts of mixed solvent composed of methanol and dichloromethane in a volume ratio of 3:1, adding 2-6 parts of 40-60wt% formalin, stirring at room temperature in a nitrogen atmosphere for 6-8 hours, evaporating under reduced pressure to remove the solvent, and carrying out silica gel chromatographic separation to obtain the fluorescence molecular probe. The invention has the advantages of high fluorescence quantum yield, low sensitivity to solvent polarity, high chemical/light stability and the like.

The invention discloses a method for preparing nano silver and titanium dioxide thin films on the surfaces of hollow glass beads, which comprises the following steps of: I, firstly coating a nano silver thin film on the surface of a hollow glass bead by using a chemical plating method; and II, then directly coating a layer of nano titanium dioxide thin film on the surface of the hollow glass bead coated with the nano silver thin film by using a hydrothermal method. The method disclosed by the invention has the advantages of raw material saving, simplicity and convenience in operation, good binding strength, higher photocatalytic activity and the like, and endows hollow glass beads with a photocatalytic degradation property for dyes and organic pollutants.