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Method for detecting quantum dot mark fast immune chromatographic test paper bar

A technology of rapid immunization and chromatography test paper, applied in the field of detection, can solve the problems of many interference factors, low detection sensitivity, and not simple method, etc., and achieve the effect of good luminescence stability, simple and fast method, and simple method

Inactive Publication Date: 2006-08-02
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The patent needs to use two kinds of particles for labeling, and the method is not simple enough; quantitative detection is carried out by using the degree of quenching of the fluorescent signal, which has many interference factors and low detection sensitivity

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0024] Dissolve cadmium acetate in oleic acid containing trioctylphosphine, then add metal selenium powder (Cd2+ / Se2-=1:2) dissolved in trioctylphosphine, stir and react at 140°C for 5min-60min, and Samples are taken at regular intervals, and centrifuged to obtain CdSe quantum dots with different particle sizes (1.2nm-6.6nm). According to the different particle sizes of the quantum dots, a yellow to dark brown-red solution can be obtained. CdSe quantum dots are dispersed and stored in organic solvents such as n-hexane, chloroform, and toluene. The 1.5nm CdSe quantum dots are formed into aggregates by miniemulsion polymerization, and these aggregates are coated with polystyrene polymer to obtain composite particles with a particle size of about 60-70nm with quantum dots, and the polymer is modified by acrylic acid The surface of quantum dot microspheres has carboxyl functional groups. Under the action of the linker carbodiimide (EDC), the quantum dot complex microspheres with ...

Embodiment 2

[0029] Dissolve cadmium chloride in alkaline (pH 10) aqueous solution containing thioglycolic acid, add ionic tellurium source NaHTe (prepared from tellurium powder and sodium borohydride), react at 100°C for 5-240 minutes, and the reaction time varies to obtain quantum dots The particle size is different (1.8-4.2nm), the color of the solution is from green to orange and finally red, and the CdTe quantum dots with carboxyl groups on the surface are synthesized. Quantum dots with a particle size of 2.5 nanometers were selected and linked to monoclonal antibodies against β-subunit chorionic gonadotropin (β-HCG) under the action of the linker carbodiimide (EDC), stirred and reacted at room temperature for 2 hours, and used Sephadex G-200 chromatographic column separation and purification to obtain quantum dot-labeled antibodies. Spray the HCG monoclonal antibody marked with quantum dots evenly on the glass fiber membrane, spread 4 square centimeters per milliliter of solution, se...

Embodiment 3

[0034] Dissolve cadmium chloride in an acidic (pH4) aqueous solution containing mercaptoethylamine, add ionic tellurium source NaHTe (prepared from tellurium powder and sodium borohydride), and react at 100°C for 5-240 minutes. The reaction time is different to obtain quantum dots. The particle size is different (2.5-5.5nm), the color of the solution is from gray to red and finally purple, and CdTe quantum dots with amino groups on the surface are synthesized. Select quantum dots with a particle size of 4 nanometers, mix water-soluble quantum dots coated with mercaptoethylamine and glutaraldehyde in phosphate buffer (0.05MpH=7.4), and add a certain amount of anti-formazine to it Fetal protein (AFP) monoclonal antibody, stirred and reacted at room temperature for 4 hours, separated and purified with a Sephadex G-200 chromatographic column to obtain the anti-AFP monoclonal antibody labeled with quantum dots; the CdTe quantum dot with carboxyl groups on the surface synthesized by ...

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Abstract

The present invention relates to a detection method of quantum point labeled quick immunochromatographic test paper strip, belonging to the field of detection technology. Said invention is characterized by that the antibody of quantum point labeled objective material can be coated on the glass fibre membrane, another antibody of objective material and second antibody are respectively coated on the nitrocellulose membrane or nitrocellulose / acetyl cellulose mixed membrane to form detection band and quality control band, on the polyester or plastic plate the glass fibre membrane and nitrocellulose membrane can be made into the immunochromatographic test paper strip. Said invention also provides the concrete steps of said detection method by utilizing said test paper strip and the concrete application range of said test paper strip.

Description

technical field [0001] The invention relates to a method in the technical field of detection, in particular to a detection method for a quantum dot-labeled rapid immunochromatographic test strip. Background technique [0002] The existing colloidal gold-labeled immunochromatographic analysis method has been widely used in clinical medical testing. After adding the sample to be tested, the target in the sample is combined with the antigen or antibody coated on the membrane through the percolation of the microporous membrane or the capillary siphon, and the colloidal gold marker reacts with it to form the color of the colloidal gold itself (red), the test results can be observed with the naked eye. However, for some samples with extremely low antigen or antibody content, the color of colloidal gold is very light, it is difficult to judge the result with the naked eye, and the sensitivity is low. Quantum dots are a type of semiconductor nanoparticle with a radius smaller than...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/544G01N33/577G01N21/64
Inventor 高峰贺蓉崔大祥潘碧峰
Owner SHANGHAI JIAO TONG UNIV
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