593 results about "Agglutination" patented technology

Microfluidic methods and devices for heterogeneous binding and agglutination assays are disclosed, with improvements relating to mixing and to reagent and sample manipulation in systems designed for safe handling of clinical test samples.

An immunodiagnostic testing apparatus includes a centrifuge and an imager disposed in relation to the centrifuge wherein at least one image is captured of at least one test element in advance of a complete centrifugation period in order to provide predictive data concerning the presence of an agglutination reaction or failure mode of the apparatus or test element.

Microfluidic cartridges for agglutination reactions are provided. The cartridges include a microfluidic reaction channel with at least two intake channels, one for an antigen-containing fluid and the other for an antibody-containing fluid, conjoined to a reaction channel modified by incorporation of a downstream flow control channel. At low Reynolds Number, the two input streams layer one on top of the other in the reaction channel and form a flowing, unmixed horizontally-stratified laminar fluid diffusion (HLFD) interface for an extended duration of reaction. Surprisingly, the design, surface properties, and flow regime of microfluidic circuits of the present invention potentiate detection of antibody mediated agglutination at the stratified interface. Antigen:antibody reactions involving agglutination potentiated by these devices are useful in blood typing, in crossmatching for blood transfusion, and in immunodiagnostic agglutination assays, for example.

The invention discloses a multichannel multiobjective hyper-spectral imaging method based on numerical microscopes, characterized in that, an object is imaged on a slit plane, an emergent light is collimate into a parallel light, split into ultraviolet light, infrared light and visible light, which are diffracted into the dispersion through the respective split grating diffraction, then is focused on the corresponding numerical microscope; the turning state of the numerical microscope is controlled by the computer, and the emergent light of the on-state is projected on the detector, used for image and post-treatment through the data acquisition treatment. The device is arranged with a multi-agglutination prism of the dichroism filter, and the dispersion of the ultraviolet light, the infrared light and the visible light is realized. The method realizes the image information acquisition of the triband of infrared light, visible light and ultraviolet light, solves the problems that the spectrum imaging data are too large without affecting the spectrographic detection quality of the target area, and is in favor of realizing the multiobjective identification and the real-time track.

An immunoassay method in which blood can be measured even without pretreatment by a centrifuge etc. In the present invention, antibodies or antigens in a sample are subjected to agglutination reaction with insoluble carriers onto which antigens or antibodies specifically reacting with the antibodies or antigens in the sample have been imrobilized and the resulting agglutination mixture is determined for the change in its absorbance or in its scattered light by irradiation with light, wherein said sample is whole blood and the whole blood is forcibly lysed.

The invention relates to a yolk-shell structure tin dioxide-nitrogen-doped carbon material and a preparation method thereof, belonging to the technical field of lithium ion battery electrode material. The yolk-shell structure SnO2@void@N-C material takes porous submicron tin dioxide SnO2 as a core and has the diameter of 200-400 nanometers; nitrogen-doped carbon (N-C) is taken as a shell, and the thickness of the shell is 15-20 nanometers; a cavity has the inner diameter of 300-500 nanometers; in the N-C shell, the mass percent of N element is 8-12%. The porous SnO2 core shortens the lithium ion diffusion path; the volume change of SnO2 can be effectively buffered by a gap between the SnO2 core and a carbon layer in the charge-discharge process, and the N-C can effectively improve the electrical conductivity of the material, so that the yolk-shell structure tin dioxide-nitrogen-doped carbon material has excellent electrochemical cycle stability. The thickness of the carbon layer can be regulated and controlled by controlling the concentration of dopamine or the auto-agglutination time, and the size of the gap can be regulated and controlled by controlling the quantity of tetraethoxysilane; the preparation method can well control the structure of the material, and is simple in technology and convenient to operate.

The invention provides methods for preparing pharmaceutical formulations for injection such that upon injection the formulation does not cause erythrocyte agglutination, hemolysis, and / or cell shrinkage. To prevent agglutination, a pharmaceutical formulation ready for injection needs to have a sufficient ionic strength. To prevent hemolysis or cell shrinkage, a pharmaceutical formulation ready for injection needs to be about isotonic with respect to plasma. The invention provides methods that prepare pharmaceutical formulations for injection that have both the sufficient ionic strength to prevent agglutination and the requisite tonicity to prevent significant hemolysis or cell dehydration or shrinkage. The present methods involve the use of sodium chloride solutions that are about 25 mM to about 150 mM for reconstituting lyophilized cakes (or other non-liquid pharmaceutical formulations) into solution or for diluting pharmaceutical formulation solutions.

The invention provides a lab device for evaluating the hi-pressure and hi-temperature cementing quality of mud cakes, which essentially comprises a hi-temp and hi-pressure vessel 19, a simulated wellhole 13, an intermediate container 22, a heating and a cooling system, a pressure source and a pressure system, a data acquisition controller 23, a computer 30 and an output equipment 31. The function of the invention is to simulate the in-well pressure and temperature condition, realize such continuous experimental operations as well-hole type filtering of drilling fluid, rinsing and replacing of drilling fluid, injecting of well-solidifying fluid and curing of well-solidifying fluid, etc., simulate and evaluate the impact of in-well drilling fluid and the existing of mud cake on the cementing quality of the interface of the well-solidifying fluid, evaluate the flushing effects of different flushing fluids on circular space, measure the shearing and cementing strength of the interface between the flushing fluid and the circular space, measure the hydraulic cementing strength of the interface between the flushing fluid and the circular space, and provide experimental data for the optimal selection and engineering design for the drilling fluid and well-solidifying fluid in well drilling in oil field.

The invention provides a micro-fluidic chip for glycosylated hemoglobin immunodetection in serum, comprising a sample introduction pool, a micro-fluid channel, a reaction tank, a detection tank, a waste liquor tank and a pump valve interface, wherein the sample introduction pool, the micro-fluid channel, the water liquor tank and the pump valve interface are serially connected by the micro-liquid channel; antigens or antibodies necessary for protein detection are fixed in the reaction and detection tanks in advance, a serum sample solution to be detected successively flows in the reaction tank by virtue of the sample introduction pool and the micro-fluid channel by an externally connected pump valve system, completes antigen or antibody specific reaction in the reaction tank and agglomerates, and the reaction product is subjected to absorbancy analysis to obtain the immune agglutination reaction and detection of the sample to be detected. The chip is convenient for sample introduction, less in sample consumption, high in reaction efficiency and short in detection time.

The invention discloses a surface modification method for a polymer separation membrane. Under the water solution condition, a dopa compound is easily oxidized by dissolved oxygen in water to generate auto-agglutination-composite reaction in order to generate a firmly attached dopa compound composite layer on the surface of the polymer separation membrane; the dopa compound composite layer contains rich catechol radicals which can form the multi-point hydrogen bonding effect with lactam radicals in polyvinylpyrrolidone, and the polyvinylpyrrolidone is firmly complexed on the surface of the separation membrane; and the polymer separation membrane immobilized with povidone-iodine on the surface can be prepared through the complexation between the polyvinylpyrrolidone and iodine. The method has simple technique, and is suitable for the polymer separation membranes with different materials and shapes; and the modified polymer separation membrane has excellent hydrophily, blood compatibility, contamination resistance and bacterial resistance, so that the important significance is given to improve the comprehensive performance of the polymer separation membrane.

A screening device for performing an immunoassay test to detect the presence of a compound in a body fluid. The device includes a holder for removably receiving a membrane to which the fluid has been applied. A light is directed to the membrane. A photodetector measures the concentration of the light reflected back from the membrane. Specifically, the concentrations of reflected light from a control zone and a test zone are measured. Signals representative of the measured light concentrations are applied to a processor. If a specified concentration of predetermined light from a control zone on the membrane is detected, the processor considers the test to be successful. In the test is successful, the processor, based upon the measured concentration of reflected light from the test zone, generates data representative of the presence of the compound.

An immunodiagnostic test card includes a plurality of transparent chambers wherein each chamber includes a quantity of testing material that combines with a patient sample, when mixed, to produce an agglutination reaction. A plurality of indicia are disposed to aid in the manufacture and determining the usability of the cards prior to test and also in objectively grading the agglutination reactions that are formed or lack of agglutination.

The invention relates to a method for fast testing human ABO / Rh / MN blood type and a kit used in the method. The method and the testing kit follow the principle of immunochromatography and realize the blood type test by observing whether residual red substances resulted from the agglutination of erythrocytes exist or not after the antigen antibody reaction of blood type substances. The method has simple operation and can avoid various defects in present ordinary blood type testing methods. In the method, the loading amount of tested blood sample is no more than 10 microliter, and the whole test process lasts no more than 2 minutes.

Ultrasound-assisted particle agglutination assay methods and apparatuses are described based on first providing a standing wave ultrasound field at a resonance frequency of a test liquid in a resonator cell containing microparticles covered with a binding agent with high affinity to an analyte sought to be detected by the assay test. Formation of the specifically-bound and nonspecifically-bound aggregates of these microparticles is then followed by effective stirring of the liquid with swept-frequency sonication causing disintegration of nonspecifically-bound aggregates and leaving specifically-bound aggregates in place for further detection and measurement. The methods and devices of the invention allow significant improvement in the sensitivity and specificity of agglutination tests and are advantageously applicable to detecting various proteins, DNA, RNA and other biologically active substances. Specific examples are provided.

A method is provided for measuring inhibition of platelet aggregation by a thrombin receptor antagonist. First, a blood sample is obtained from a patient treated with a thrombin receptor antagonist. The blood sample is mixed in combination with particles including an immobilized GPIIb / IIIa receptor ligand and a thrombin receptor activator. The combination is then incubated under conditions suitable for agglutinating the particles, and platelet-mediated agglutination is assessed in the mixture. The absence of agglutination indicates that the patient has reduced ability to form platelet thrombi in response to the thrombin receptor antagonist treatment. Also provided is a kit for measuring inhibition of platelet aggregation by a thrombin receptor antagonist that includes a GPIIb / IIIa receptor ligand immobilized on a particle, a thrombin receptor activator, an anticoagulant, and a buffer to maintain the anticoagulated blood in a condition suitable for platelet aggregation.

The invention relates to the field of biomedicine, discloses a CD47 nanobody and an application thereof, and particularly discloses a blocking type nanobody for integrin-associated protein (CD47) andderived protein thereof. Particularly, the invention discloses an integrin-associated protein (CD47) binding molecule and an application thereof, particularly in treatment and / or prevention or diagnosis of CD47 associated diseases such as tumor. The related CD47 nanobody can effectively block interaction between the CD47 and a ligand SIRPa thereof, and the antibody cannot cause human erythrocyte agglutination.

The invention relates to the technical field of the lithium ion battery diaphragm production, and specifically relates to a SiO2-PS nuclear shell structure ceramic diaphragm coating. A preparation method of the SiO2-PS nuclear shell structure ceramic diaphragm coating comprises the following steps: after modifying the monodispersed nanometer SiO2-particle in-situ grafted gamma-methacryloyloxy trimethoxy silane (MPS) prepared through the St-ber method, coating the monodispersed nanometer modified SiO2 particles by adopting the polyvinyl pyrrolidone ethanol solution and styrene-azodiisobutyronitrile mixed solution, thereby forming the nuclear shell structure with stable combination effect, and preparing the SiO2-PS nuclear shell structure ceramic diaphragm coating slurry; the nuclear shell SiO2-PS composite particle with good dispersibility can be uniformly dispersed in the coating slurry system and cannot produce excessive agglutination, and can form the coating not hindering the polyolefin porous diaphragm gap after being coated on the polyolefin porous diaphragm, and has good combining effect with the polyolefin porous diaphragm, and the membrane has good mechanical performance onthe whole; the diaphragm has stable membrane form under high-temperature state, the SiO2 of the nuclear layer is free from ceramic powder falling and blocking diaphragm duct under the coating of theglass-state polymer when the heat shrinkage phenomenon of the diaphragm caused by the high temperature is effectively blocked, and the security of the lithium ion battery is improved.