33 results about "Particle agglutination" patented technology

A particle agglutination-evaluating container for immunological analysis, based on an agglutinate formed in an agglutination reaction between an antibody- or antigen-containing sample and agglutination particles, includes a transparent container body having a bottom face including a sloping bottom face and a raised bottom face extended in a horizontal direction at the top of the sloping bottom face to form an obtuse angle with the sloping bottom face, and a fluidal separation layer containing insoluble particles which are filled in the container body and where the agglutination particles are separated according to the agglutination degree, wherein agglutination is evaluated by observation from the bottom of the container body, where the agglutination reaction is judged positive when the agglutination particles are observed through the raised bottom face, negative when observed at the bottom end of the sloping bottom face, and weakly positive when observed in the middle of the sloping bottom face.

The present invention provides: a reagent and a kit for an insoluble carrier particle nephelometric immunoassay which stabilize the agglutination reaction by suppressing the action of blood plasma components that are involved in the agglutination reaction of insoluble carrier particles such as latex and affect the values to be determined, to provide the stable absorbances of the reaction solutions and the accurate determination results; and a method of an insoluble carrier particle nephelometric immunoassay utilizing said reagent or kit. An antigen or an antibody is carried on an insoluble carrier particle in the presence or absence of a buffer solution comprising a compound having within its molecule a group shown below such as bicine, tricine, then an antibody- or antigen-sensitized insoluble carrier particle suspension is allowed to contact a sample in the presence of the above mentioned buffer solution to cause the immune agglutination reaction, and the turbidity generated by the insoluble carrier particle agglutination reaction is measured to determine the antigen or antibody in the sample (wherein R<1>, R<2> and R<3> may be the same or different, and independently represent a hydrogen atom, a hydroxyalkyl group or the like).

Latex particles for a particle agglutination assay, with which it is possible to carry out high-sensitivity assay while highly suppressing a non-specific reaction are disclosed. The latex particles being obtained by emulsion polymerization with a polymerizable monomer having a phenyl group, and a polymerizable monomer having a phenyl group and a sulfonate in an aqueous medium including a nonionic surfactant at a concentration of 0.005 to 0.02 wt % based on the aqueous medium, the latex particles having an average particle size of 0.005 to 1.0 μm.

The invention discloses an inert carrier indirect agglutination test detection system and its application. The indirect agglutination test detection system comprises an inert carrier bacterium S9 and a complex S9-P expressing and carrying P antigen factors on its surface. The inert carrier indirect agglutination test detection system only carries the P antigen factor, has a single component, and is specifically targeted. It will produce a positive particle agglutination reaction visible to the naked eye under certain concentration conditions with the whole blood or serum of chickens infected with pullorum and Salmonella gallinarum typhi. However, non-specific cross-agglutination reactions did not occur in chicken-derived serum and whole blood with different backgrounds of non-pulmonary pullorum and Salmonella gallinarum infection. The S9‑P is based on the glass plate agglutination reaction operation platform, which is easy to operate, sensitive and fast, the agglutination reaction particles are visible to the naked eye, and the results are clear and easy to judge. The test and result judgment can be completed within two minutes. The S9‑P is suitable for chickens in chicken flocks The target-specific detection of pullorum and Salmonella gallinarum typhi infection has a good application prospect in the field monitoring and diagnosis of chicken flocks.