33 results about "Particle agglutination" patented technology

A method for detecting and / or observing particle agglutination comprising: placing a volume of particle suspension on a filter constructed to allow passage of individual particles; placing a volume of solution or suspension containing an agglutination reagent on the particle The location of the suspension; optionally placing the washing solution at the location of the agglutinating reagent; and observing the surface of the location for the presence of particles, which presence indicates that particle agglutination has occurred. Also provided are methods for detecting agglutination in general and hemagglutination, eg, for blood typing and cross-matching in particular. The method consists of continuous vertical addition of whole blood, blood typing reagents, and detergent to the filter. In the case of hemagglutination, colored, preferably red or reddish spots appear after washing. Devices and kits based on the present invention are also claimed which facilitate blood typing and cross matching in a non-laboratory environment without the need for laboratory instruments.

Ultrasound-assisted particle agglutination assay methods and apparatuses are described based on first providing a standing wave ultrasound field at a resonance frequency of a test liquid in a resonator cell containing microparticles covered with a binding agent with high affinity to an analyte sought to be detected by the assay test. Formation of the specifically-bound and nonspecifically-bound aggregates of these microparticles is then followed by effective stirring of the liquid with swept-frequency sonication causing disintegration of nonspecifically-bound aggregates and leaving specifically-bound aggregates in place for further detection and measurement. The methods and devices of the invention allow significant improvement in the sensitivity and specificity of agglutination tests and are advantageously applicable to detecting various proteins, DNA, RNA and other biologically active substances. Specific examples are provided.

This invention relates to the use of thermodynamically incompatible surfaces in agglutination assays for the express purpose of using the sample as a key component of the detection instrument. Specifically, the invention relates to formation of a lense and a virtual container for rapid mixing via thermal energy by a sample liquid disposed on a superhydrophobic surfaces, and a subsequent specific analyte or overall protein concentration assay using particles agglutination for use in the industrial, environmental, and clinical laboratory test fields.

A method for the detection and / or visualization of particle agglutination in a particle suspension is provided, comprising placing a volume of the particle suspension and a volume of a solution or suspension containing an agglutinating agent at substantially the same selected location on a surface of a filter constructed so as to permit passage of individual unagglutinated particles in a direction perpendicular to the surface. A wash solution at optionally placed at substantially the same location as that of said agglutinating agent, and the surface is observed for the presence of particles. There is also provided a method for detection of agglutination reactions, such as used in blood grouping, reverse grouping and cross-matching. A device and a kit based on the invention is also claimed, which facilitates blood grouping, reverse grouping and matching in non-laboratory environment without the need for laboratory instruments.

A particle agglutination-evaluating container for immunological analysis, based on an agglutinate formed in an agglutination reaction between an antibody- or antigen-containing sample and agglutination particles, includes a transparent container body having a bottom face including a sloping bottom face and a raised bottom face extended in a horizontal direction at the top of the sloping bottom face to form an obtuse angle with the sloping bottom face, and a fluidal separation layer containing insoluble particles which are filled in the container body and where the agglutination particles are separated according to the agglutination degree, wherein agglutination is evaluated by observation from the bottom of the container body, where the agglutination reaction is judged positive when the agglutination particles are observed through the raised bottom face, negative when observed at the bottom end of the sloping bottom face, and weakly positive when observed in the middle of the sloping bottom face.

The invention belongs to the bird immunology technology area, it concretely relates to a kit of acceptable for the one type duck hepatitis virus antibody emulsion agglutination and the application of testing one type duck hepatitis virus antibody emulsion agglutination. The kit of this invention contains box body and core reagent in the box body, positive check sample, negative check sample, sample cell, scale sample sucker and the carrier of observed result. The characteristic is that core reagent is latex antigen from sublimating e duck hepatitis virus AV2111 and inactivating one type duck hepatitis virus sensitization polystyrene latex, the positive check sample is one type duck hepatitis virus antiserum, the negative check sample is the green duck of a day old of no one type duck hepatitis mother source antibody. The carrier is the paper characteristic latex agglutination card of having hollowness. The invention also discloses the preparation method and application of this kit. The kit has the significance merit of high specificity, high sensitivity, handy operation, and quickly diagnose.

The present invention provides: a reagent and a kit for an insoluble carrier particle nephelometric immunoassay which stabilize the agglutination reaction by suppressing the action of blood plasma components that are involved in the agglutination reaction of insoluble carrier particles such as latex and affect the values to be determined, to provide the stable absorbances of the reaction solutions and the accurate determination results; and a method of an insoluble carrier particle nephelometric immunoassay utilizing said reagent or kit. An antigen or an antibody is carried on an insoluble carrier particle in the presence or absence of a buffer solution comprising a compound having within its molecule a group shown below such as bicine, tricine, then an antibody- or antigen-sensitized insoluble carrier particle suspension is allowed to contact a sample in the presence of the above mentioned buffer solution to cause the immune agglutination reaction, and the turbidity generated by the insoluble carrier particle agglutination reaction is measured to determine the antigen or antibody in the sample (wherein R<1>, R<2> and R<3> may be the same or different, and independently represent a hydrogen atom, a hydroxyalkyl group or the like).

Latex particles for a particle agglutination assay, with which it is possible to carry out high-sensitivity assay while highly suppressing a non-specific reaction are disclosed. The latex particles being obtained by emulsion polymerization with a polymerizable monomer having a phenyl group, and a polymerizable monomer having a phenyl group and a sulfonate in an aqueous medium including a nonionic surfactant at a concentration of 0.005 to 0.02 wt % based on the aqueous medium, the latex particles having an average particle size of 0.005 to 1.0 μm.

The invention provides a mixing method and a mixing apparatus for particle agglutination. A mixing method for particle agglutination includes the following steps of: dropping testing materials into anaccommodating recess at one end of a channel structure; pressing a flexible layer on the other end of the channel structure; and releasing the flexible layer to an initial position after pressing theflexible layer such that a negative pressure is generated by an air chamber that is covered by the flexible layer and draws the testing materials that are in the accommodating recess to move toward the air chamber along a diverging channel of the channel structure. The testing materials are mixed with each other in the diverging channel, in which the depth of the diverging channel is gradually increased from the accommodating recess to the air chamber.

The invention discloses an inert carrier indirect agglutination test detection system and its application. The indirect agglutination test detection system comprises an inert carrier bacterium S9 and a complex S9-P expressing and carrying P antigen factors on its surface. The inert carrier indirect agglutination test detection system only carries the P antigen factor, has a single component, and is specifically targeted. It will produce a positive particle agglutination reaction visible to the naked eye under certain concentration conditions with the whole blood or serum of chickens infected with pullorum and Salmonella gallinarum typhi. However, non-specific cross-agglutination reactions did not occur in chicken-derived serum and whole blood with different backgrounds of non-pulmonary pullorum and Salmonella gallinarum infection. The S9‑P is based on the glass plate agglutination reaction operation platform, which is easy to operate, sensitive and fast, the agglutination reaction particles are visible to the naked eye, and the results are clear and easy to judge. The test and result judgment can be completed within two minutes. The S9‑P is suitable for chickens in chicken flocks The target-specific detection of pullorum and Salmonella gallinarum typhi infection has a good application prospect in the field monitoring and diagnosis of chicken flocks.