The invention provides a kit for measuring adiponectin by a nano latex enhanced immune turbidimetry. The kit comprises reagent R1, reagent R2 and adiponectin antigen calibration material solution, wherein the reagent R1 comprises electrolyte, a stabilizer, a surface active agent, a preservative and buffer solution; the reagent R2 comprises latex particles coated by anti-human adiponectin polyclonal antibody, electrolyte, a stabilizer, a surface active agent, a preservative and buffer solution; the latex particles are copolymer formed by styrene and acrylic acid; the adiponectin antigen calibration material solution comprises adiponectin and a stabilizer; the surfaces of the latex particles in the reagent R2 have carboxyl groups, and the particle size range of the latex particles is 80-100nm. The kit is simple to operate, rapid and accurate in measurement, high in sensitivity and specificity, and low in detection cost.