The invention relates to a preparation method of a stable
glycated albumin calibrating material and a
quality control material. The preparation method comprises the following steps of selecting serum, and removing
settling and disturbance matters; adding glucose and
preservative into the treated serum, arranging in a constant-temperature water bath with temperature of 35-38 DEG C, saccharifying, and respectively obtaining the
glycated albumin calibrating material and the
quality control material according to the saccharifying time; respectively packaging the prepared
glycated albumin calibrating material and the
quality control material, arranging in dialysates with temperature of 2-6 DEG C, dialyzing, and removing excessive glucose, until the content of the remained glucose is less than 0.1g / L; finally, adding the
preservative and 10-50g / L of freeze-
drying protecting agent into the dialyzed glycated
albumin calibrating material and quality
control material, freezing and
drying to obtain a freeze-
drying powder, dissolving again, and detecting the fixed value by a glycated
albumin detection kit. The preparation method has the advantages that the sources of raw materials are sufficient, easy to obtain, the preparation technology is simple, and the stable glycated
albumin calibrating material and the quality
control material can be prepared by the serum of a healthy person.