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Stable kit for detecting glycation albumin

A glycated albumin and detection kit technology, which is applied in the field of glycated albumin detection kits, can solve the problems of large human error, low cost, inaccurate measurement results, etc., and achieve the effect of accurate detection methods and accurate results.

Active Publication Date: 2014-12-10
上海睿康生物科技有限公司
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Problems solved by technology

By using a specific monoclonal antibody to highly specifically recognize a specific non-glycosylated glycosylation region, measure the content of non-glycosylated serum albumin at this specific position, and indirectly obtain the content of glycated albumin, although this method is relatively low in cost, but The measurement results are not accurate enough, and there are large human errors in the operation process

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  • Stable kit for detecting glycation albumin
  • Stable kit for detecting glycation albumin
  • Stable kit for detecting glycation albumin

Examples

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Embodiment 1～3

[0039] The present examples 1-3 relate to a stable glycosylated albumin detection kit, which consists of reagent R1, reagent R2 and reagent R3. The components and concentrations of the reagents R1, R2 and R3 are shown in Table 1:

[0040] Table 1

[0041]

[0042] The glycated albumin detection kit described in Examples 1-3 of the present invention is applicable to various types of automatic biochemical analyzers, taking Hitachi 7170 automatic biochemical analyzer as an example, its operations are shown in Table 2 and Table 3.

[0043] Glycated albumin analysis method: two-point endpoint method, that is, reagent R1; the dosage of R2 is 240ul and 60ul respectively, and the sample size is 3ul; add 3ul sample to 240ul reagent R1, read point A1 after 5min at 37°C, add 60ulR2, after 5min, Read point A2; the detection wavelengths are respectively main wavelength 546nm and subwavelength 700nm.

[0044] Albumin analysis method: one-point endpoint method, that is, the amount of rea...

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Abstract

The invention provides a stable kit for detecting glycation albumin. The stable kit consists of a reagent R1, a reagent R2 and a reagent R3, wherein the reagent R1 comprises the following components: a buffering solution, glycation amino acid oxidase, albumin protease, peroxidase, catalase, 4-aminoantipyrene, a stabilizing agent and a preservative; the reagent R2 comprises the following components: a buffering solution, albumin protease, peroxidase, chromogen, a stabilizing agent and a preservative; the reagent R3 comprises the following components: a buffering solution, bromocresol green, a surface active agent and a preservative. The stable kit provided by the invention has the advantages that the influence on the detection result caused by endogenous glycation amino acid is removed by adding glycation amino acid oxidase, so that the result is accurate; the stable kit adopts an enzyme method to detect the content of the glycation albumin, and is a simple, fast, sensitive and accurate detection method.

Description

technical field [0001] The invention relates to the field of medical immune in vitro diagnosis, in particular to a stable glycated albumin detection kit. Background technique [0002] Glucose in serum undergoes a non-enzymatic glycosylation reaction with the N-terminus of serum protein to generate glycosylated serum protein, and glycosylated albumin accounts for more than 90% of glycosylated serum protein. In addition, since the content of albumin exists stably in serum, while globulin will fluctuate due to infection and other disease factors; therefore, accurate determination of glycosylated albumin is very important to accurately reflect the blood sugar control of diabetes. [0003] Glycated albumin is an indicator that reflects the average blood sugar level over the past 2-3 weeks. The reaction period of glycated hemoglobin, the "gold standard" of blood sugar testing, is shorter. Therefore, glycosylated albumin has advantages over glycosylated hemoglobin in the confirma...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 李伟奇李杰房君江张秀文林清玉
Owner 上海睿康生物科技有限公司
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