259 results about "Glycated hemoglobin" patented technology

Compositions and assay methods for and relating to the detection of glycated hemoglobin are provided. In particular, the presence and level of glycated hemoglobin can be detected in a blood sample. The compositions comprise microparticles which have zinc coating for use in the improved binding of proteins exhibiting a zinc binding domain, in particular glycated hemoglobin.

A system for treating a blood sample (700) having an analyte of interest comprises a strip (200) having a membrane (218), respective portions (216, 220 and 222, or 300) which are provided for receiving the sample, for lysing cells of the sample to liberate hemoglobin, and for capturing glycated hemoglobin. The latter two portions (220 and 222, or 300) of the membrane are treated with lysing and capture agents, respectively. A portion of the strip (214 or 230 or 240) is provided for holding an eluting agent and for releasing the agent upon a release condition. A system for detecting analyte comprises an optical subsystem (550) that is aligned with the strip to provide a signal corresponding to an amount of analyte, and an electronic subsystem (650) for processing the signal (560) to provide a result, such as an amount or percentage of glycated hemoglobin. To use these systems, the user simply applies a small sample (700) to the membrane (218) and closes a door (10) of the detection system over the strip (200) such that the door triggers the release of the eluting agent. No sample pre-treatment is required. The preferably handheld system (100) is a simple and convenient monitoring tool for the user, such as a diabetic patient who must monitor blood glucose on an on-going basis. While the systems are useful in the monitoring of blood glucose, they may be used for treating a sample other than blood and detecting an analyte other than an analyte in blood.

A method of determining glycated hemoglobin is provided, by which a ratio of the glycated hemoglobin in a sample can be determined accurately and easily. The ratio of glycated hemoglobin can be determined by degrading a glycated hemoglobin in a whole blood sample selectively with a protease to give a glycated hemoglobin degradation product`; causing a redox reaction between a glycation site of the glycated hemoglobin degradation product and a fructosyl amino acid oxidoreductase; and determining this redox reaction. Further, as shown in FIG. 1, in a whole blood sample, there is a correlation between the ratio of the glycated hemoglobin determined by this method and an HbA1c concentration. Thus, without determining the glycated alpha-amino group as a characteristic structure of HbA1c, an amount of HbA1c can be determined accurately and easily from the determined ratio of the glycated hemoglobin.

Compositions for accurately assaying a glycated protein by: 1) avoiding effects of globulin and ascorbic acid components, 2) siabilizing proteases and at least enzymes acting on glycated amino acids; 3) accurately assaying albumin; and 4) assaying glycated albumin while avoiding the effects of glycated hemoglobin, and an assay method are provided. Thus, the contents of a glycated protein and glycated albumin can be more accurately determined.

The invention relates to a four-gradient elution analysis method of a glycated hemoglobin analyzer, which is used for detecting concentration of glycated hemoglobin via ionic exchange chromatography. The method comprises the following steps of: setting four kinds of eluent with fixed concentration; respectively using the four kinds of eluent to elute cation exchange resin having an absorbed sample to be detected; and performing chromatographic analysis on the eluted liquid to determine the content of the eluted ingredients, thereby determining a blood glucose concentration, wherein the four kinds of eluent are respectively as follows: an eluent A for washing and balancing the cation exchange resin, an eluent B for eluting other subfractions including HbA1a and HbA1b of the hemoglobin, an eluent C for eluting HbA1c subfraction of the glycated hemoglobin, and an eluent D for eluting an non-glycated hemoglobin and regenerated cation exchange resin. The method provided by the invention can accurately detect the concentration of the glycated hemoglobin, simplify structures of detection devices and detection programs, improve operational reliability of devices, decrease cost on devices and reduce cross contamination of the eluent.

Standard reference materials and related methods are described for quality testing and calibration of instruments used for the qualitative and quantitative determination of hemoglobin and glycated hemoglobin as well as other analytes of interest in animal tissue samples. The reference solutions of the disclosure utilize a synthetic cruor in combination with an Hb peptide chain.

The invention relates to a method for measuring glycosylated hemoglobin content, which comprises the following steps of: a, uniformly mixing a whole blood sample and hemolytic liquid; b, adding an appropriate amount of hemolyzed sample into latex suspending in glycine buffer solution; c, respectively adding an antibody B reagent and an antibody A reagent after preset time; d, uniformly mixing andreading a scattering value through a specific protein analyzer under the condition of single-wavelength light irradiation; and e, reading the glycosylated hemoglobin content from a standard curve diagram according to the scattering value. The glycosylated hemoglobin content of the blood sample can be directly determined without separately measuring the total hemoglobin content and the glycosylated hemoglobin content of the blood sample by taking fixed-time nephelometry as a detection principle; and antibody working solution does not need preparing in advance, so that the effective service time of the reagents is prolonged, the operating steps are simplified and the measuring time of the sample is shortened.

A method of treating of glycosylated hemoglobin and in particular the process of hemoglobin isolation and preparation of a stable glycosylated hemoglobin in which the glycosylated hemoglobin remains liquid. The method comprises steps including separating human red blood cells from anti-coagulated blood, washing the human red blood cells with physiological saline and centrifuging the red blood cells and aspirating and discarding a resulting supernatant and white blood cell layer, lysing the packed red blood cells, mixing and freezing the cell/water mixture, defrosting, centrifuging, filtering and saving the supernatant, heating the supernatant, diafiltering the adjusting the hemolysate so that a final hemoglobin concentration is within specified limits. Additives may include potassium cyanide, carbon monoxide, and appropriate preservatives.

The invention relates to an enzymatic test kit for glycosylated hemoglobin, which is composed of hemolysis buffer solution, reagent R1a, reagent R1b and reagent 2; wherein, the hemolysis buffer solution is 10 to 1000mmol/ L N- cycloethyl-2-aminoethanesulfonic acid, 10 to 1000mmol/L MOPS and 1 to 100g/L polyethylene glycol oxide dodecyl ether; the reagent R1a is 100 to 10000 kU/L metalloproteinase, 0.1 to 10mmol/L 2-(iodophenyl)-3-(2, 4- dinitrobenzene)-5-(2, 4-disulfophenyl)-2H tetrazole sodium salt; the reagent R1b is 0.1 to 10mmol/L MES and 0.5 to 50mmol/L CaCl2; the reagent 2 is 1 to 100kU/L fructose valine oxidase with high activity, 30 to 600kU/L POD, 0.01 to 0.5mmol/L DA-64 and 10 to 1000mmol/L Tris-HCl. The test kit coincides with the characteristics of rapidness, large batch size and economy which are required by the glycosylated hemoglobin clinical tests.

The invention provides a one-step type integrated blood glucose meter comprising a blood glucose meter body, a detecting box in an accommodating groove of the blood glucose meter body and a glycosylated hemoglobin detecting unit. The blood glucose meter body is provided with a control circuit. The detecting box is provided with a blood glucose detecting unit corresponding to a blood collecting port and a guide structure allowing the blood glucose detecting unit provided with a blood collecting needle and a blood glucose test strip to collect blood movably. The blood glucose meter body is provided with a drive mechanism connected to the control circuit, and the drive mechanism is used for driving the blood glucose detecting unit to move by sensitive triggering to allow the blood colleting needle to penetrate the blood collecting port to collect blood and return. A glycosylated hemoglobin detecting position is formed on the surface of the blood glucose meter body through the glycosylated hemoglobin detecting unit. The blood glucose meter body further integrally corresponds to a wireless receiving unit, connected to a wireless emitting unit of a blood glucose sensor planted subcutaneously, and a glucose interpreting unit which is connected to the wireless receiving unit and which is used for dynamically converting and displaying blood glucose values. The blood glucose meter integrates functions of blood glucose detection, dynamic blood glucose monitoring and glycosylated hemoglobin detection, and is small in size and convenient to operate.

The present invention provides an electrochemical detection method for content of glycosylated hemoglobin in the blood, and is characterized in that: whole blood specimen is diluted by combined liquid ranging from 30 times to 70 times and passes through a glycosylated protein recognizer, electric activated small molecules are filled into the effluent liquid from the glycosylated protein recognizer, and electrochemical response of the liquid is detected by chemically modified electrodes after the filling, thereby obtaining the concentration of the blood glucose. The glycosylated protein recognizer is eluted by elute solvent, and the electrochemical response of the elute solvent is detected and the concentration of the glycosylated hemoglobin is obtained, thereby obtaining the content of the glycosylated hemoglobin of the sample after calculation. As the electrochemical detection is simple in operation, lower in cost and rapid in the analysis, the present invention is expected to realize the rapid determination of the clinical glycosylated hemoglobin and the rapid determination of the glycosylated hemoglobin after separation and elution.

The present invention is drawn to a method of measuring glycated hemoglobin, which can comprise steps of establishing multiple age-specific groups of red blood cells, and measuring HbA_1c levels of at least one of said groups. In another embodiment, a system for measuring glycated hemoglobin can comprise a separating device configured to separate red blood cells into multiple age-specific groups, and a measuring device configured to measure HbA_1c levels of at least one of said groups.