Electrochemical biosensor for direct determination of percentage of glycated hemoglobin

A technology of glycosylated hemoglobin and biosensors, which is applied in the field of electrochemical biosensors and can solve problems such as target molecular dysfunction

Inactive Publication Date: 2011-05-25
GENERAL ATOMICS CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This reaction leads to the dysfunction of the target molecule through the formation of sugar adducts and cross-linking

Method used

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  • Electrochemical biosensor for direct determination of percentage of glycated hemoglobin
  • Electrochemical biosensor for direct determination of percentage of glycated hemoglobin
  • Electrochemical biosensor for direct determination of percentage of glycated hemoglobin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Example 1: By pipetting the sample onto the biosensor or immersing the biosensor in the sample Electrochemical sensing of HbA1c

[0105] Prepare a biosensor with working, reference, and counter electrodes. Both the working electrode and the counter electrode were Ir-carbon modified; and the reference electrode was an Ag / AgCl electrode. Working and counter electrodes were prepared by screen printing on polyester substrates. The electrical connections of these electrodes were silver and the electrodes were first printed with silver ink. The Ir-carbon ink was further printed on the silver-based electrodes. Ir-carbon inks were prepared by mixing and homogenizing 0.5-2 g of Ir-carbon with 5-30 ml of an ink-based solution (containing polyethyleneimine and 2-hydroxyethylcellulose). The working electrode also contains fructosyl amino acid oxidase. A fructosyl amino acid oxidase (such as FVO having the amino acid sequence shown in SEQ ID NO: 1 ) ink was prepared by mixin...

Embodiment 2

[0109] Example 2: Electrochemical sensing of HbA1c using a biosensor with a capillary filling device

[0110] The biosensors described in Example 1 were hand laminated to form capillary filling devices. See Figure 4 .

[0111] The biosensor was immersed in the incubation solution of Example 1 for a few seconds to fill the microchannels with hemolysate by capillary action. The biosensor is then used for electrochemical measurements. Electrochemical measurements were performed for 120 seconds at +0.25 V vs. Ag / AgCl reference electrode. The measured current for each sample is shown in Figure 7 middle. Figure 7 The data in demonstrate that the current measured from the biosensor fits a linear model with the percentage HbA1c in the blood sample, and that the biosensor and the method can be used to determine the percentage of HbA1c without measuring total hemoglobin alone.

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Abstract

The invention provides electrochemical biosensors for direct determination of percentage of glycated hemoglobin in blood samples without the need of a separated measurement of total hemoglobin content in blood samples. The invention provides methods for using the electrochemical biosensors.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of priority to Provisional Patent Application US Serial No. 61 / 052,888, filed May 13, 2008, which is hereby incorporated by reference in its entirety. field of invention [0003] The present invention relates to an electrochemical biosensor for measuring the percentage of glycated hemoglobin (HbA1c) in a blood sample without measuring total hemoglobin alone. The HbA1c biosensor can be used for diabetes monitoring in a doctor's office or a patient's home. Background of the invention [0004] Glycated protein is a substance produced by non-enzymatically and irreversibly bonding amino groups of amino acids constituting proteins with aldehyde groups of reducing sugars such as aldose. See, eg, US Patent No. 6,127,138. Such a non-enzymatic and irreversible binding reaction is also called "Amadori rearrangement", so the above-mentioned glycosylated protein may also be called "Amadori comp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/00G01N33/543G01N33/72
CPCG01N33/5438G01N33/723C12Q1/005G01N2333/906
Inventor 尼尔·K·布卢袁崇生阿比吉特·达塔刘利民方磊
Owner GENERAL ATOMICS CO
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