The invention discloses a
transaminase mutant and an application thereof. An
amino acid sequence of the
transaminase mutant is the
amino acid sequence obtained by mutating the
amino acid sequence shown as SEQ ID NO: 1, and the
mutation includes at least one of the following
mutation sites: 3rd, 5th, 8th, 25th, 32nd, 45th, 56th, 59th, 60th, 84th, 86th, 164th, 176th, 178th, 180th, 187th, 197th, 206th, 207th, 242th, 245th, 319th, 324th sites and the like; alternatively, the amino acid sequence of the
transaminase mutant has the
mutation site in the amino acid sequence in which the mutation is generated. The application of the mutant can increase the
reaction rate, improves the stability of the
enzyme, reduces the amount of the
enzyme, and reduces the difficulty of the post-treatment, so thatthe transaminase mutant can be suitable for industrial production.