99results about How to "Improve enzyme stability" patented technology

The present invention relates to a hybrid enzyme comprising carbohydrate-binding module amino acid sequence and a fungal alpha-amylase amino acid sequence and to a variant of a fungal wild type enzyme comprising a carbohydrate-binding module and an alpha-amylase catalytic module. The invention also relates to the use of the hybrid enzyme or the variant in starch liquefaction.

A novel class of pharmaceuticals which comprises a Locked Nucleic Acid (LNA) which can be used in antisense therapy. These novel oligonucleotides have improved antisense properties. The novel oligonucleotides are composed of at least one LNA selected from beta-D-thio / amino-LNA or alpha-L-oxy / thio / amino-LNA. The oligonucleotides comprising LNA may also include DNA and / or RNA nucleotides.

We have found that long standing problems relating to the stability of organic materials in alkaline detergents, including the reversion or hydrolysis of condensed phosphate sequestrants in alkaline solid detergent compositions can be alleviated by the use of an organic composition having vicinal hydroxyls. Such a solid block detergent can be manufactured by a process in which a source of alkalinity, a functional material including a condensed phosphate sequestering agent, an organic compound having two vicinal hydroxyl groups, are combined in a pourable composition or liquid. Such a liquid can be introduced into a plastic capsule and solidified. During manufacture and solidification, we have found that the vicinal hydroxyl compound prevents substantial hydrolysis or reversion of the condensed phosphate maintaining effective hardness ion sequestration during the use of the detergent. The stabilizer can also stabilizes color, chlorine content and dispensing properties.

Liquid laundry detergent compositions are provided that show remarkable performance even though they utilize only eco-friendly ingredients and have a sustainability index of greater than 3. Some embodiments include a liquid laundry detergent composition comprising alkyl polyglycoside (APG) with fatty alcohol sulfate, at least two detersive enzymes, an enzyme stabilization system (e.g. borate and / or citrate and / or calcium salts), d-limonene or other natural essence, water and adjuvant. In another exemplary embodiment, APG is combined with fatty acid soaps, at least two detersive enzymes, an enzyme stabilization system (e.g. borate and / or citrate and / or calcium salts), d-limonene or other natural essence, water and adjuvant. Such compositions show remarkable performance, good viscosity, physical storage stability, enzyme stability, and have a sustainability index of greater than 3.

A biosensor in which at least one reagent constitutes a portion of a working electrode, a conductive track leading from a working electrode to an electrical contact associated with a working electrode, or an electrical contact associated with a working electrode. For example, the biosensor can have a mediator or an enzyme or both incorporated into the working electrode itself. Other reagents can be dispensed on the electrode itself either directly or by impregnating a matrix, such as a mesh or a membrane, with the enzyme, and then placing the impregnated mesh or membrane over the electrode. Alternatively, the biosensor can have a mediator or an enzyme or both incorporated into the conductive track leading from the working electrode to an electrical contact associated with the working electrode. In another alternative, the biosensor can have a mediator or an enzyme or both incorporated into the electrical contact associated with the working electrode itself. Furthermore, the biosensor can have a mediator or an enzyme or both incorporated into at least two of the foregoing components of the biosensor.

Formulations for pitch control, and methods of making and using thereof, are described herein. The formulations contains one or more 1,3-selective lipases. 1,3-selective lipases catalyze the hydrolysis of the terminal ester groups in triglycerides leaving the internal ester group intact. The enzyme formulations can contain one or more additives, such as dispersants, metal ions, absorbents, adsorbents, cationic polymers, and combinations thereof. The enzyme formulation is typically applied as a solution to the pulp stock. The enzyme formulations can be applied at any of one or more various points during the pulping and paper manufacturing processes. The use of selective lipase(s) decreases the total concentration of fatty acids in the system, and catalyzes the formation of monoglycerides, which are more effective at dispersing fatty acids than glycerol, the product of non-selective lipases, thereby improving pulp and paper machine runnability and pulp and paper quality.

The present invention is directed to novel oligonucleotides with improved antisense properties. The novel oligonucleotides comprise at least one Locked Nucleic Acid (LNA) selected from beta-D-thio / amino-LNA or alpha-L-oxy / thio / amino-LNA. The oligonucleotides comprising LNA may also include DNA and / or RNA nucleotides. The present invention also provides a new class of pharmaceuticals which comprise antisense oligonucleotides and are useful in antisense therapy.

A detergent composition comprising; a) a non-phosphorous based builder which is an amino acid based compound or a succinate based compound, b) one or more enzymes which are destabilised by the non-phosphorous containing builder and c) a stabilisation system for the one or more enzymes. The stabilising system comprising one or more divalent metal ion salts and a non-ionic surfactant. Preferably the builder is the tetrasodium salt of glutamic-N,N-diacetic acid and the enzyme comprises protease. The preferred divalent metal ion salts are calcium salts.

An aqueous liquid cleaning composition having a pH of at least 7, preferably from 7 to 11, more preferably from 7 to 10 and comprising from 1% to 90% by weight of surfactant, a proteolytic enzyme and a primary stabiliser therefor, the composition further comprising an organic substance which forms a complex with a transition metal, the complex being capable of catalysing bleaching of a substrate by atmospheric oxygen.

Injectable insulin loaded microgels that are capable of modifying the amount of insulin released based on the patient's tissue glucose levels, methods for making and using these compositions have been developed. The microgels contain insulin, glucose oxidase entrapped in or bound to the microgels, and an agent that reduces hydrogen peroxide, entrapped in or bound to the microgels, wherein the polymeric microgel expands when pH decreases from physiological pH and shrinks when pH increases towards physiological pH, thereby releasing insulin at a rate corresponding to the glucose concentration. In one embodiment, the glucose oxidase and / or the agent reducing hydrogen peroxide are encapsulated in nanogels, then encapsulated within the microgel.

Disclosed is a modified glucose dehydrogenases that has dramatically increased productivity in Escherichia coli and dramatically increased thermal stability, which is obtained by introducing specific amino acid mutations to glucose dehydrogenase derived from Botryotinia fuckeliana. Also disclosed is a modified glucose dehydrogenases that has dramatically increased productivity in E. coli and dramatically increased thermal stability, which is obtained by replacing two amino acid residues in glucose dehydrogenase of fungal origin with cysteine residues. The novel glucose dehydrogenase has a low reactivity to xylose.

Protein-polymer compositions and processes for their production are provided where the compositions have improved resistance to ultraviolet light induced weathering and associated loss of enzyme activity. The incorporation of both a sterically hindered amine and an ultraviolet light absorber at a concentration of at least 5% by weight produces a curable or cured composition with UV stabilized enzyme activity that can be used as a bioactive coating with improved stain removal properties.

Disclosed are detergent compositions for hand dishwashing which, by incorporating certain organic solvents, results in a liquid dishwashing detergent composition that is not only a more effective cleaning agent, but also offers improved physical and enzymatic stability and more convenient rheology and handling characteristics than typical liquid dishwashing compositions.

Methods and compositions are provided for enhanced signal intensity and storage stability of standard nucleic acid amplification buffers, real-time PCR buffers or both. Buffers in accordance with the present invention include anti-freeze protein(s) (AFPs), optionally with a carrier protein, such as BSA.

A phosphate-free automatic dishwashing detergent composition comprising:a) a coated bleach particle comprising a core substantially consisting of bleach and a coating layer enclosing this core and adhering thereto substantially consisting of sodium sulphate; andb) a protease, amylase or a mixture thereof.wherein the composition is preferably free of anionic and / or cationic surfactants.

A biosensor in which at least one reagent constitutes a portion of a working electrode, a conductive track leading from a working electrode to an electrical contact associated with a working electrode, or an electrical contact associated with a working electrode. For example, the biosensor can have a mediator or an enzyme or both incorporated into the working electrode, into the conductive track leading from the working electrode to an electrical contact associated with the working electrode, and / or into the electrical contact associated with the working electrode. Other reagents can be dispensed on the electrode itself either directly or by impregnating a matrix, such as a mesh or a membrane, with the enzyme, and then placing the impregnated mesh or membrane over the electrode.

The present invention relates to stabilization of asparaginase enzymes.

The present invention relates to a sensing electrode of an enzyme-based sensor, and the enzyme-based sensor comprising the same can be stably stored at room temperature. The sensing electrode comprises: an electrode substrate and an enzyme sensing layer formed thereon, wherein the enzyme sensing layer comprises sequentially laminated layers of: a first carbon material-nano metal layer containing a carbon material and nano-metal particles; an ionic liquid layer comprising an ionic liquid consisting of a cation and an anion; a second carbon material-nano metal layer containing a carbon material and nano-metal particles; and an enzyme layer. The present invention also provides a method for manufacturing the sensing electrode of an enzyme-based sensor.

Wood pulp is treated with an esterase formulation in combination with a metal ion or cationic polymer to increase the stability or activity or both of esterase enzymes at high temperature, or at extreme pH ranges of acidic and alkaline conditions. The treatment by esterase together with metals ion or cationic polymer can be used to treat pitch containing pulp at high temperatures prior to, during or after refining of wood chip / pulp, in order to enhance the reduction of pitch problems and facilitate in the manufacture of paper.

The invention discloses a high-protein distiller's grain feed and a preparation method thereof. With distiller's grain and silkworm pupa powder as main materials, the preparation method for the high-protein distiller's grain feed utilizes a variety of strains ( such as phanerochaete chrysosporium, trichoderma reesei and aspergillus niger) to carry out step-by-step fermentation, and thereby lignin,cellulose and hemicellulose in distiller's grain are deeply degraded in succession. The invention effectively controls factors (such as choice and proportioning of carbon source and nitrogen source,oxygen input, intermediate degradation product, enzyme activator and inhibitor) in the process of microbial fermentation degradation, increasing the degradation rate of crude fibers, the utilization rate of distiller's grain and the feeding value of the distiller's grain feed. According to the high-protein distiller's grain feed prepared by the fermentation method disclosed by the invention, the crude fiber content is decreased by 12 to 15 percent, the crude protein content is increased by 25 to 28 percent, and the nutrition of the high-protein distiller's grain feed is closer to the nutritionof complete feed.

The invention discloses lipase made of aspergillus niger strains, and a producing method and utilization thereof. The lipase (Aspergillus niger) AN0512 is preserved in the China general microbiological culture collection center which is called CGMCC for short on November 19th, 2012, and the preserving number is CGMCC No.6835. The producing method includes: fermenting the lipase in a culture medium using olive oil as a main carbon source; removing thalluses to obtain lipase crude enzyme liquid; and then depositing and dialyzing, and separating the lipase crude enzyme liquid through anion exchange chromatography and molecular exclusion chromatography to obtain electrophoresis pure lipase. The lipase has a wide temperature application range, good activity when hydrogen ion concentration ranges from 4.0 to 6.0, and good acid resistance, and can be used in industries of food processing, drug preparing, chemical synthesizing and the like.

A phosphate-free automatic dishwashing detergent composition comprising:a) a coated bleach particle comprising a core substantially consisting of bleach and a coating layer enclosing this core and adhering thereto substantially consisting of sodium sulphate; andb) a protease, amylase or a mixture thereof.wherein the composition is preferably free of anionic and / or cationic surfactants.

The invention discloses a transaminase mutant and an application thereof. An amino acid sequence of the transaminase mutant is the amino acid sequence obtained by mutating the amino acid sequence shown as SEQ ID NO: 1, and the mutation includes at least one of the following mutation sites: 3rd, 5th, 8th, 25th, 32nd, 45th, 56th, 59th, 60th, 84th, 86th, 164th, 176th, 178th, 180th, 187th, 197th, 206th, 207th, 242th, 245th, 319th, 324th sites and the like; alternatively, the amino acid sequence of the transaminase mutant has the mutation site in the amino acid sequence in which the mutation is generated. The application of the mutant can increase the reaction rate, improves the stability of the enzyme, reduces the amount of the enzyme, and reduces the difficulty of the post-treatment, so thatthe transaminase mutant can be suitable for industrial production.

A method of making didanosine (ddI) including the steps of: (a) obtaining an enzyme expressing ddA deaminase activity; (b) immobilizing the enzyme onto an insoluble support; (c) contacting the enzyme with a dideoxyadenosine (ddA) solution of at least about 4% weight volume ddA in water for a time and under conditions to produce a ddI solution; and (d) isolating the ddI from the ddI solution. Optionally, the ddI mother liquor is reused in subsequent runs to improve yield.

Stabilized use solutions of low phosphorus, alkali metal carbonate detergents employing enzymes for cleaning compositions are disclosed. In particular, the present invention is a composition for, and method of, removing soils, preventing redeposition of protein soils and reducing foam, using stabilized enzyme cleaning compositions, namely use solutions of the same.

The invention discloses a preparation method of a novel bromelain preparation. The preparation method comprises the following steps: (1) preparing pineapple juice; (2) preparing a natural polyanion polysaccharide solution; (3) respectively adjusting the pineapple juice and the natural polyanion polysaccharide solution until the pH values are the same, adequately mixing the pineapple juice with the natural polyanion polysaccharide solution, and stirring to generate a bromelain-polyanion polysaccharide compound precipitate through an electrostatic attraction interaction between the bromelain and polyanion polysaccharide in the pineapple juice; and (4) centrifugally collecting the bromelain-polyanion polysaccharide compound precipitateand drying, so as to obtain the bromelain preparation. By utilizing a principle of generating the precipitate by virtue of the electrostatic attraction interaction between the bromelain and polyanion polysaccharide, the bromelain is separated, and the bromelain preparation is obtained. The bromelain preparation has the stability of bromelain in storage and processing processes and has good slow-release and control-release properties, and the disadvantages that bromelain is sensitive to head and easy to oxidize can be overcome.

The invention provides an isonucleoside compound or an ortho-phosphite derivative thereof. The compound can be used for effectively increasing the gene silencing efficiency of oligonucleotide modified by the compound and keeping the enzyme stability of the oligonucleotide. The invention further provides a preparation method of the isonucleoside compound or the ortho-phosphite derivative thereof, a solid-phase synthesis method of oligonucleotide 5' end phosphorylation and the application of the compound to the modification of the oligonucleotide.