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Kit for measuring glycolated serum albumin by enzymatic chemiluminescence method

A technology of serum albumin and luminescence method, which is applied in the field of kits for the determination of the concentration ratio of glycated serum albumin to albumin by enzyme chemiluminescence, can solve the problems of complex construction of protease co-expression body, unfavorable promotion, high cost, etc., and achieve improvement Specificity, high precision of test results, and the effect of improving accuracy

Active Publication Date: 2018-04-03
GUANGZHOU JINDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, albumin in blood samples is different from albumin at a purified reagent level, and various substances such as bilirubin and lipoproteins are bound, so it is difficult to obtain fragments that fructoaminoacidase can easily act on by proteases
Therefore, when measuring albumin in samples such as serum or plasma, it is necessary to add a large amount of protease to the sample to improve reactivity, but this causes the following problems: high cost, and the presence of a large amount of protease, resulting in Other enzymes used in the assay system of glycosylated albumin, such as catalase, ascorbate oxidase, bilirubin oxidase, etc., are inactivated
However, the construction of the protease co-expression body in the reagent is relatively complicated, and the cost is high, which is not conducive to popularization

Method used

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  • Kit for measuring glycolated serum albumin by enzymatic chemiluminescence method
  • Kit for measuring glycolated serum albumin by enzymatic chemiluminescence method
  • Kit for measuring glycolated serum albumin by enzymatic chemiluminescence method

Examples

Experimental program
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Effect test

Embodiment 1

[0065] Embodiment 1 Enzyme chemiluminescence assay kit for glycosylated serum albumin

[0066] Example 1 The kit for measuring glycated serum albumin by enzymatic chemiluminescence method includes reagent R1, reagent R2 and reagent R3, wherein the reagent R1 comprises the following components: urea phosphate 10 mmol / L, triethanolamine 1 mmol / L, pancreatic Protease 1U / mL, anti-interference agent 0.02%, proclin 300 0.05%, pH 7.5 MES buffer 10mmol / L, the anti-interference agent is composed of chloroacetyl chloride and N-chloroacetamide in a mass ratio of 1:0.5 .

[0067] The reagent R2 comprises the following components: 8 U / mL of fructosamine oxidase, 10 g / L of trehalose and 10 mmol / L of pH7.5 phosphate buffer.

[0068] The reagent R3 comprises the following components: amino acid oxidase 20U / mL, trehalose 10g / L and pH7.5 phosphate buffer 10mmol / L.

[0069] The reagent R4 is a commercially available luminescence substrate 1,2-dioxetaneboronic acid.

Embodiment 2

[0070] Embodiment 2 Enzyme chemiluminescence assay kit for glycosylated serum albumin

[0071] Example 2 The kit for measuring glycated serum albumin by enzymatic chemiluminescence method includes reagent R1, reagent R2 and reagent R3, wherein the reagent R1 comprises the following components: 30 mmol / L of urea phosphate, 3 mmol / L of triethanolamine, pancreatic Protease 1U / mL, anti-interference agent 0.01%, proclin 300 0.06%, pH 8.0 MES buffer 30mmol / L, the anti-interference agent is composed of chloroacetyl chloride and N-chloroacetamide in a mass ratio of 1:0.6 .

[0072] The reagent R2 comprises the following components: fructosamine oxidase 10 U / mL, trehalose 20 g / L and pH 8.0 phosphate buffer 20 mmol / L.

[0073] The reagent R3 comprises the following components: amino acid oxidase 30 U / mL, trehalose 20 g / L and pH 8.0 phosphate buffer 20 mmol / L.

[0074] The reagent R4 is a commercially available luminescence substrate 1,2-dioxetaneboronic acid.

Embodiment 3

[0075] Example 3 Kit for Determination of Glycated Serum Albumin by Enzyme Chemiluminescence

[0076] Example 3 The kit for measuring glycated serum albumin by enzymatic chemiluminescence method includes reagent R1, reagent R2 and reagent R3, wherein the reagent R1 comprises the following components: 40 mmol / L of urea phosphate, 4 mmol / L of triethanolamine, pancreatic Protease 1U / mL, anti-interference agent 0.03%, proclin 300 0.04%, pH 8.5 MES buffer 40mmol / L, the anti-interference agent is composed of chloroacetyl chloride and N-chloroacetamide in a mass ratio of 1:0.6 .

[0077] The reagent R2 comprises the following components: fructosamine oxidase 20U / mL, trehalose 20g / L and pH8.5 phosphate buffer 40mmol / L.

[0078] The reagent R3 comprises the following components: amino acid oxidase 50 U / mL, trehalose 20 g / L and pH 8.5 phosphate buffer 20 mmol / L.

[0079] The reagent R4 is a commercially available luminescence substrate 1,2-dioxetaneboronic acid.

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Abstract

The invention belongs to the technical field of medical examination and particularly relates to a kit for measuring a concentration ratio of glycolated serum albumin and albumin. The kit comprises a reagent R1, a reagent R2, a reagent R3 and a reagent R4, wherein the reagent I contains a protein denaturant, hydroxyl-alkyl amine, trypsin, an anti-interference agent, a preservative and a buffer solution; the reagent II contains fructose amino acid oxidase, trehalose and a buffer solution; the reagent III contains amino acid oxidase, trehalose and a buffer solution; and the reagent IIII is a luminescent substrate 1,2-dioxetane boronic acid. The kit provided by the invention is similar with an existing enzymatic method detection kit in detection principle, but is lower in cost, high in interference resistance, accurate in detection, stable in property and convenient for extensive use.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a kit for measuring the concentration ratio of glycated serum albumin to albumin by an enzyme chemiluminescence method. Background technique [0002] In the human body, glucose and protein undergo a non-enzymatic glycation reaction to form glycated protein. Fructosamine (GSP) is the product of non-enzymatic reaction between serum protein (mainly albumin) and glucose. Since the half-life of albumin is 17-19 days, its value can reflect the average level of blood glucose in 2-3 weeks before the measurement , is currently clinically used to judge short-term blood sugar control indicators. However, since this reaction can also occur with non-specific reducing substances in serum, and the non-enzymatic glycation reaction rates of different protein components are different, the specificity of GSP detection method is poor. The detection of glycosylated serum albumin (GA) is a q...

Claims

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Application Information

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IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 李民友蓝媛蔺涛张玲齐宗献
Owner GUANGZHOU JINDE BIOTECH
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