30 results about "Fructosamine" patented technology

The present invention is directed to a single test system and method for determining the integrated glycemic condition of a subject by measuring the concentration of glucose and the level of protein-bound glucose in a subject's body fluid, such as whole blood. The glucose concentration is indicative of the subject's immediate glycemic condition, whereas the protein-bound glucose concentration is indicative of either intermediate or long-term glycemic condition. Optionally, other analytes indicative of glycemic condition, such as ketone bodies or fatty acid derivatives, can also be measured. The present invention also provides a method of diagnosing diabetes. The invention additionally provides a method for analyzing the concentration of fructosamine in less than or equal to five minutes without the use of a reaction accelerator.

The invention relates to a process flow to produce a sesquiterpene inside a golden fungus fermentation liquid and the virtue of the sesquiterpene produced by the golden fungus to reduce the blood sugar, belonging to the biological fermentation and medicine field. The process flow is characterized in that the process flow applicant finds the fermentation liquid and the sesquiterpene produced by the production process are applied on a high blood sugar mouse through an oral administration route, the mouse significantly reduces the blood sugar and the fructosamine, which approves that the golden fungus fermentation liquid and the sesquiterpene are possessed of preparing a health care drink and medicine to reduce the blood sugar; the fermentation liquid color is orange to yellow-brown; the inhibitive rate to the ex vivo non-enzymic glycosylation is up to 72% to 90% with the fermentation liquid and 5mg / ml sesquiterpene. The process flow to produce a sesquiterpene inside a golden fungus fermentation liquid and the virtue of the sesquiterpene produced by the golden fungus to reduce the blood sugar has the advantages of not only creating a fire-new channel to further develop the golden fungus but also providing an ideal Chinese herbal medicine to reduce blood sugar.

The invention relates to a tumor specific growth factor detection kit. The kit comprises an R1 reagent, an R2 reagent and a standard substance solution, the R1 reagent comprises phosphate, disodium ethylene diamine tetraacetate, Kathon and 4-(2-hydroxyethyl)-1-piperazinepropanesulfonic acid, and the solvent of the R1 reagent is purified water; the R2 reagent comprises a developer, Kathon, sodium citrate and glycerin, and the solvent of the R2 reagent is purified water; and the standard substance solution comprises a solution A and a solution B, the solution A is purified water, and the solution B is a sialic acid, homocysteine and fructosamine mixed solution. The kit has the advantages of substantial simplification of detection steps, cost saving, simple practical operation, no need of a precious device, and extremely easy popularization.

The invention discloses a 6-fructosamine-4-arylamidoquinazoline derivative with the structural formula of formula I or a pharmaceutically acceptable salt thereof, and relates to a preparation method and a purpose of above compounds. According to the invention, fructose is connected to a 4-arylamidoquinazoline derivative, so the water solubility of the compounds and the selectivity of the compounds to tumor cells are improved.

The invention provides a fructosamine determination kit having the following formula of 100mmol / L of R1 trismetyl aminomethane, 50mmol / L of sodium carbonate, 5g / L of Triton100 (TX-100), 1g / L of sodiumazide and PH of 10.8. Through long-term experimental observation, the reagent is good in stability and can be placed for 18 months without precipitation by using an INT substrate.

The invention discloses a fructosamine determination kit. The kit comprises a reagent R1 and a reagent R2, wherein the pH value of the reagent R1 is 10.0-10.8, and the reagent R1 comprises 200-500mmol / L of an alkaline buffer solution, 1-10g / L of a metal salt, 0.5-2g / L of a mixture of Emulgen A90 and Emulgen B66 with the same volume ratio, and 0.5-2g / L of a preservative; and the pH value of the reagent R2 is 4.0-5.0, and the reagent R2 comprises 5-25mmol / L of an acidic buffer solution, 0.5-2g / L of isopropylamine alkylbenzene sulfonate, 1-5g / L of NBT and 0.5-2g / L of a preservative. According tothe invention, the mixture of Emulgen A90 and Emulgen B66 with the same volume ratio is specially selected from the reagent R1 and is matched with a strong alkaline environment, so that interferents in a serum sample are effectively removed; in addition, an acid environment and isopropylamine alkylbenzene sulfonate are matched for use in the reagent R2, the concentration of the buffer solution isproperly reduced to enhance the solubility of NBT and the stability of the reagent, and meanwhile, the precision and linear range of the reagent are improved, so that the fructosamine determination kit with remarkably enhanced anti-interference capability and stability and more excellent reagent linearity and precision is obtained.

The invention relates to a fructosamine deglycase pichia pastoris expression vector, a genetically engineered bacterium, a construction method and a protein expression method, and belongs to the technical field of protein expression. The fructosamine deglycase pichia pastoris expression vector disclosed by the invention comprises a skeleton vector and a gene for coding fructosamine deglycase; and the nucleotide of the gene for coding the fructosamine deglycase is as shown in SEQ ID NO. 1. The vector disclosed by the invention is beneficial to industrial production of the fructosamine deglycase, is green in production method, safe, environment-friendly, pollution-free and controllable, is beneficial to large-scale preparation of the glucosamine, and can generate greater social value and economic benefits.

Synthesis of fructosamine perfume compound and flavoring use of tobacco are disclosed. It is carried out by taking fructose and ammonia water as material and catalytic synthesizing in sodium hydrate-methyl alcohol solution system. It achieves low cost, simple synthetic process and high output. It can be used for additive of tobacco.

The invention discloses a 6-fructosamine-4-arylamidoquinazoline derivative with the structural formula of formula I or a pharmaceutically acceptable salt thereof, and relates to a preparation method and a purpose of above compounds. According to the invention, fructose is connected to a 4-arylamidoquinazoline derivative, so the water solubility of the compounds and the selectivity of the compounds to tumor cells are improved.

The invention provides a fructosamine determination kit, the formula of the kit is: R1 trimethylolaminomethane 100mmol / L, sodium carbonate 50mmol / L, triton 100(TX-100) 5g / L, stack Nitrogen sodium 1g / , PH 10.8 and through long-term experimental observation, using INT substrate, the reagent stability is good, and there is no precipitation phenomenon after 18 months.

The invention discloses a fructosamine determination kit, which includes R1 reagent and R2 reagent, wherein the pH of the R1 reagent is 10.0-10.8, including: 200-500mmol / L alkaline buffer solution, 1-10g / L metal salt, 0.5- 2g / L mixture of Emulgen A90 and Emulgen B66 in equal volume ratio, 0.5-2g / L preservative; R2 reagent pH 4.0-5.0, including: 5mmol / L-25mmol / L acidic buffer, 0.5-2g / L isopropylamine phenyl sulfonate; 1~5g / L NBT and 0.5~2g / L preservative. The present invention specially selects the equal volume ratio mixture of Emulgen A90 and Emulgen B66 in the R1 reagent, cooperates with the strong alkaline environment to effectively remove the interfering substances in the serum sample; and in the R2 reagent, the acidic environment and isopropylamine alkyl The benzenesulfonate is used in conjunction with it, and the concentration of the buffer is appropriately reduced to enhance the solubility of NBT and the stability of the reagent, and at the same time improve the precision and linear range of the reagent, thus obtaining a significantly enhanced anti-interference ability and stability , and the linearity and precision of the reagent are also more excellent fructosamine determination kit.

The present invention relates to a kit for detecting fructosamine. The kit comprises a first reagent containing a carbonate buffer, a second reagent containing nitrotetrazole blue and an anionic surfactant; a calibration product. The anionic surfactant is selected from one or more of a phosphate anionic surfactant and a sulfonate anionic surfactant, and optionally, the kit is used for detecting fructosamine in blood plasma. According to the kit of the invention, the consistency of detection results of serum and plasma samples during fructosamine detection is improved. The invention also relates to a method for detecting fructosamine and application of the anionic surfactant in improving the consistency of fructosamine detection results.

A method for predicting the degree of weight loss attainable by applying one or more dietary interventions to a subject, said method comprising determining the level of one or more biomarkers in one or more samples obtained from the subject, wherein the biomarkers are selected from fructosamine and factor VII.

A method for predicting the degree of weight loss attainable by applying one or more dietary interventions to a subject, said method comprising determining the level of one or more biomarkers in one or more samples obtained from the subject, wherein the biomarkers are selected from fructosamine and factor VII.

The invention discloses stereum hirsutum fermentation liquor and sesquiterpene which are produced by fermenting stereum hirsutum liquor, as well as applications thereof, and particularly relates to a production technology process of sesquiterpene cultured and obtained by the stereum hirsutum liquor, and the hypoglycemic efficiency of sesquiterpene. Repeated tests and study show that a culture solution produced by the production technology contains sesquiterpene, a mouse with hyperglycemia takes the culture solution orally, and then blood sugar and fructosamine of the mouse can be reduced remarkably, therefore, the stereum hirsutum culture solution is confirmed to have the application for preparing healthy drinks and medicines capable of reducing the blood sugar. The culture solution is liquor with the color ranging from aurantium to tawny, and the sesquiterpene content in the culture solution is 0.4 mg / mL-4.0 mg / mL. According to the invention, not only is a new way explored for the deep development and utilization of the stereum hirsutum, but also an ideal traditional Chinese medicine is provided for controlling the blood sugar.