760 results about "Ursolic acid" patented technology

The invention discloses a slowly-released compound acidifier for poultry and livestock feed, a preparation method thereof and feed containing the same. The compound acidifier comprises the following compositions in part by weight: 30 to 80 parts of complex organic acid, 15 to 50 parts of supplementary materials and 5 to 20 parts of coating agents. The complex organic acid is citric acid, fumaric acid, malic acid, lactic acid, linoleic acid, crataegolic acid, ursolic acid, chlorogenic acid, glycyrrhizic acid and oleanolic acid, and the supplementary materials are silicon dioxide, hydroxypropyl methylcellulose and ethyl cellulose. The acidifier is scientifically proportioned, has strong efficiency and is long-acting and slow-releasing, nutrients in the feed are free from being damaged, the acidifier has good fluidity and is easy to be uniformly mixed with the feed, the digestive absorption of nutrients can be promoted, the conversion rate of the feed can be improved, the productivity of poultry and livestock can be improved, the time for domestic animals for sale can be effectively shortened, the health of gastrointestinal mucosa of the animals can be protected, the immunologic function of the animals can be strengthened, and diseases can be prevented.

The invention relates to the technical field of nano materials and specifically relates to amino-modified silica with dual drug-loading effects. According to the technical scheme, firstly, mesoporous silica nanoparticles are prepared by a template method; secondly, surface amino modification is carried out onto the mesoporous silica nanoparticles by 3-aminopropyltriethoxy silane; thirdly, ursolic acid is chemically coupled to the spherical mesoporous silica nanoparticles by amido bonds; and fourthly, the coupled nanoparticles are dispersed in DMF (dimethyl formamide) solution; a proper amount of ursolic acid is added, mixed and stirred; and finally vacuum drying is carried out to obtain the amino-modified silica with dual drug-loading effects. The mesoporous nano material with dual drug-loading effects prepared by the mesoporous silica disclosed by the invention is high in loading rate, can achieve controlled release effect for the loading drug ursolic acid, so that drug effect lasts for a long period time, and therefore, bioavailability of the ursolic acid is greatly improved.

Disclosed are skin care compositions exerting a novel complementary dual mode of action in protecting the skin from day-to-day insults. The skin care compositions of the present invention comprise the extract from the liquid endosperm of Cocos nucifera as the principle ingredient along with one or more actives including the fruit rind extracts of Garcinia cambogia, Garcinia indica and Garcinia mangostana, seed coat extract of Tamarindus indicus, Chlorogenic acid extract from the beans of Coffea arabica, seed extracts of Nelumbo species, the leaf extracts of Murraya koenigii and the triterpene pentapeptides of oleanolic acid and ursolic acid. While the extract of Cocos nucifera has been included as a nutritional supplement (a natural cell culture medium) to ensure the maintenance of the viability and constant renewal of the cells of the skin, the other actives have been carefully chosen based on one or more of their effective skin care properties. The compositions of the present invention offer a safe prophylactic / therapeutic management solution to the wide range of pathological states associated with skin insults and the associated psycho-social issues.

The present invention relates to a topical skin care composition containing a safe and effective amount of a skin care active comprising agmatine, and its salt; a safe and effective amount of a first additional skin care active selected from the group consisting of BHT or BHA, hexamidine, cetyl pyridinium chloride, green tea catechins, phytosterols, ursolic acid, compounds derived from plant extracts, their salts and derivatives; and a dermatologically acceptable carrier for the agmatine composition. The present invention also relates to methods of using such agmatine compositions to regulate hair growth and the condition of mammalian skin. Said methods generally comprise the step of topically applying the composition to the skin of a mammal needing such treatment, a safe and effective amount of such compositions.

The invention relates to a Rosmarinus officinalis extract, its preparing process and use, wherein the active constituents of the extract include carnosic acid 15-40%, carnosol 5-12% and ursolic acid 14-35%. The preparing process comprises drying the Rosmarinus officinalisl medicinal material in shade, cutting into chunks, charging solvent for extracting, cooling down the merged filtrate and filtrating, decolorizing with activated charcoal, drying and disintegrating. The extract can be used for preparing medicament for treating ischemic cardiovascular and cerebrovascular diseases.

The present invention provides triterpenoids produced from natural compounds such as oleanolic acid, ursolic acid, betulinic acid, and hederagenin.

The invention relates to a natural active drug-polysaccharide targeted compound with antitumor activity and targeting property. A natural active drug is introduced onto a polysaccharide framework by chemical grafting, and physically mixed with a ligand distearoylphosphatidyl ethanolamine-polyethyleneglycol-anisoyl amine with targeted transmission capacity to form a natural active drug-polysaccharide targeted compound, and the natural active drug-polysaccharide targeted compound can be self-assembled in water to form a nano micelle. The method is characterized in that the natural active drug-polysaccharide targeted compound has targeted transmission capacity, enhances the tumor transfer efficiency of the preparation, reduces the untoward reaction and enhances the tolerance of the patient; the hydrophobic inner core formed by the hydrophobic group can physically wrap the antineoplastic drug, thereby obviously improving the water solubility of the antineoplastic drug; and the antineoplastic drug which is physically wrapped by the chemically coupled ursolic acid can implement combined treatment of tumors and lower the toxic or side effect. The preparation method is simple and easy to operate, has the advantage of higher yield, and can easily implement industrialization.

The invention discloses total triterpene extract and total phenol extract extracted from Chinese herbal medicine glossy privet fruit and a preparation method thereof. The total triterpene extract mainly contains oleanolic acid, ursolic acid, acetyl oleanolic acid, indicant compound and other derivatives with acetyl oleanolic acid as the mother nucleus. The total phenol extract mainly contains hydroxyl mandelic, 3, 4-dihydroxy phenylethyl alcohol, salidroside and indicant compound and other derivatives with salidroside as the mother nucleus. The glossy privet fruit total triterpene extract and total phenol extract can be prepared by any method of solvent distillation, solvent extraction, deposition, macroporous resin absorption, supercritical fluid extraction, column chromatography and liquid-liquid countercurrent distribution chromatography, or any combination of these methods. The total percentage of various triterpenoids of the prepared glossy privet fruit total triterpene extract is 5 to 100 percent (w / w); wherein, the content of oleanolic acid and ursolic acid accounts for 5 to 100 percent (w / w) of the total triterpene. The total percentage of various phenols of the prepared glossy privet fruit total phenol extract is 5 to 100 percent (w / w); wherein, the content of salidroside accounts for 5 to 100 percent (w / w) of the total phenol.

The invention relates to preparation and application of an amphiphilic ursolic acid-polysaccharide coupled substance with antitumor activity. The ursolic acid is chemically modified to substitute C3 site carboxyl group with amino group, the generated aminated ursolic acid is chemically coupled to a polysaccharide framework to form the amphiphilic ursolic acid-polysaccharide coupled substance, and the amphiphilic ursolic acid-polysaccharide coupled substance can be self-assembled into a nano micelle in water. The method is characterized in that the antitumor activity of the chemically modified ursolic acid is obviously enhanced; the hydrophobic inner core formed by the hydrophobic group can physically wrap the antineoplastic drug to obviously improve the solubility of the antineoplastic drug; and the antineoplastic drug which is physically wrapped by the chemically coupled ursolic acid can implement combined chemotherapy, has higher antitumor activity, has the combined synergetic action, and lowers the toxicity. The preparation method is simple and easy to operate, has the advantage of higher yield, and can easily implement industrialization.

The invention discloses a method for preparing the ursolic acid with the paulownia leaves as raw materials, comprising the following steps that: 1. the dry paulowina leaf powders with a particle size ranging from 20 to 60 meshes are taken and 3 to 5 times amount of the ethanol with a concentration over 90 percent is added in the dry paulowina leaf powders with carrying out the reflux extraction; the reflux extraction is conducted for 1 to 2 hours each time and goes for altogether 3 times; the ethanol-extracts are combined and are filtered so as to remove the impurities; the filtrates are driven to rest for over 12 hours after the ethanol is recycled and the precipitates are collected after filtration; the precipitates are dried and then the brown grey or black brown crude products of the ursolic acids are obtained. 2. the crude products of the ursolic acids are dissolved using the ethanol with a concentration ranging from 50 percent to 70 percent and the macroporous adsorption resin is used with carrying out the dynamic absorption; the solution is subject to the absorption till the solution approaches the saturated state before the solution is washed using 2 to 3 times of the deionized water by resin concentration for 3 to 5 times and then the obtained solution is eluted using the ethanol solvent with a concentration ranging from 85 percent to 99 percent; the precipitates are decolored using the activated carbon before the precipitates are crystalized for 2 to 3 times using the double solvents of the ethanol so as to obtain the target products. The advantages of the invention lie in that the goal of the industrialized batch production of the ursolic acids is realized with the paulownia leaves having wide sources as raw materials, and the purity and stability of the products both exceed 95 percent and no discharge of waste gas, waste residue and organic solvents exists in the invention.

The present invention discloses a method for extracting ursolic acid, carnosic acid and rosmarinic acid from rosemary. Reflux extraction with 95% ethanol solution for 1-2 hours, extract 2-3 times, concentrate the extract under reduced pressure to an alcohol concentration of 30-70%, let stand for crystallization for 3-7 hours, filter out the crystals and dry to obtain ursolic acid; 2) The crystallized mother liquor was concentrated under reduced pressure to no alcohol, centrifuged to obtain the solid and dried to obtain carnosic acid, and the liquid was further concentrated under reduced pressure to obtain rosmarinic acid. The method of the invention has the advantages of simple operation, high raw material utilization rate, low production cost and easy industrial production.

A UGT2B inhibitor capable of increasing the bio-availability of a drug, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: capillarisin, isorhamnetin, β-naphthoflavone, α-naphthoflavone, hesperetin, terpineol, (+)-limonene, β-myrcene, swertiamarin, eriodictyol, cineole, apigenin, baicalin, ursolic acid, isovitexin, lauryl alcohol, puerarin, trans-cinnamaldehyde, 3-phenylpropyl acetate, isoliquritigenin, paeoniflorin, gallic acid, genistein, glycyrrhizin, protocatechuic acid, ethyl myristate, umbelliferone, PEG (Polyethylene glycol) 400, PEG 2000, PEG 4000, Tween 20, Tween 60, Tween 80, BRIJ® 58, BRIJ® 76, Pluronic® F68, Pluronic® F127, and a combination thereof. A UGT2B enhancer capable of enhancing a clearance rate of morphine-like analgesic agents, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: nordihydroguaiaretic acid, wogonin, trans-cinnamic acid, baicalein, quercetin, daidzein, oleanolic acid, homoorientin, hesperetin, narigin, neohesperidin, (+)-epicatechin, hesperidin, liquiritin, eriodictyol, formononetin, quercitrin, genkwanin, kaempferol, isoquercitrin, (+)-catechin, naringenin, daidzin, (−)-epicatechin, luteolin-7-glucoside, ergosterol, rutin, luteolin, ethyl myristate, apigenin, 3-phenylpropyl acetate, umbelliferone, glycyrrhizin, protocatechuic acid, poncirin, isovitexin, 6-gingerol, cineole, genistein, trans-cinnamaldehyde, and a combination thereof.

A method for the treatment of obesity, diabetes, high cholesterol and related diseases including hyperglycemia, lipid disorders, hyperglyceridemia, dyslipidemia and atherosclerosis in mammals using anthocyanins, anthocyanidins, ursolic acid and / or betulinic acid is described. Compositions adapted for these treatments are also described.

The present invention relates to bioactivity-guided isolation and identification of bioactive compounds from oregano and mint plants, in particular, rosmarinic acid, oleanolic acid and ursolic acid, and use of these compounds or combinations thereof as anti-inflammatory agents for the treatment of conditions related to pain and inflammation and / or as ingredients of dietary supplements. The invention also relates to optimization of the methods for qualitative and quantitative analysis of the bioactive compounds in oregano and mint plants. In particular, this invention introduces an LC / MS (SIM mode) method to achieve co-quantitation of the three organic acids using a unique tandem column system. In addition, the invention also relates to the methods for recovering various water-soluble polyphenols and triterpenes from aromatic plants.

The present invention is process of extracting and separating ursolic acid from plant. The extracting and separating process of ursolic acid includes methanol reflux extraction of coarse paulownia leaf powder, recovering methanol to small volume, settling, filtering to eliminate filtrate, washing the precipitate with acetone for many times to eliminate impurity, and re-crystallization for three times in anhydrous alcohol and methanol to obtain ursolic acid. Compared with other processes of extracting ursolic acid, the present invention has the advantages of cheap material, simple technological process, low cost and being suitable for industrial production.

A composition and a method for using the composition to delay the onset of the symptoms of Alzheimer's disease in humans, comprising curcumin, piperine, oleic acid, oleanolic acid, ursolic acid, galantamine, and huperzine A, among other compounds. Curcumin is an antioxidant, while galantamine and huperzine A inhibit the activity of acetylcholinesterase in the brain. Piperine and oleic acid increase the bioavailability and gastrointestinal absorption of curcumin, galantamine, huperzine A, and other nutrients.

The invention discloses an extracting method of urson and oleanolic acid from bitter leaves, which comprises the following steps: adopting bitter leaves from Hainan as raw material; extracting through pure water; filtering; refluxing filter slag to extract through alcohol; adopting inorganic film filtering method to remove macromolecular impurity in the extract; hyperfiltering; condensing the penetration liquid through nanofiltering; adsorbing and eluting through macromolecular resin chromatographic column; condensing eluent to crystallize; obtaining rough product of total triterpenic acid; washing the rough product; dissolving in the alcohol; alkalining; acidifying; washing again; recrystallizing; obtaining the total triterpenic acid; dissolving the total triterpenic acid in the carbinol to boil; filtering; condensing filtrate; evolving; obtaining the monomer product of oleanolic acid; washing the sediment; dissolving the crystal through alcohol; obtaining the monomer product of urson; hyperfiltering and clarifying filtrate of bitter leaves; nanofiltering; condensing; adsorbing through polyamide resin selectively; eluting; condensing the eluent; obtaining the monomer of tea polyphenol with certain purity.

The invention relates to pH-responsive, folic acid-targeting and ursolic acid-supporting silica-chitosan-folic acid nano material and application. First, an antitumor drug, ursolic acid, is supported to inner ducts of carboxylic mesoporous silica; second, d partial amino groups in tumor cell-targeting molecules, folic acid and chitosan, are linked through amido bonds to prepare a conjugate; third, the conjugate is conjugated with drug-supporting carboxylic mesoporous silica through amido bonds to obtain the material. The nano support material prepared herein has uniform particle size distribution, good dispersity, large specific surface area and good biocompatibility; the defect that the ursolic acid is poor in water solubility and low in bioavailability can be solved; the release of ursolic acid can be sustained, and the release of the ursolic acid at low pH is significantly higher than that in neutral condition; this material has specific targeting for folate receptor high-expressed tumor cells, antitumor effect of ursolic acid can be further improved, and toxic and side effect upon normal tissue cells can also be reduced.

The invention relates to a ursolic acid-aspirin conjugate shown in a formula (I) and application thereof in preparing drugs for preventing tumor metastasis. In vivo experiments verify that the ursolic acid-aspirin conjugate provided by the invention has a remarkable inhibitory effect on adhesion, migration, invasion and the like of tumor cells. in vivo animal experiments verify that the ursolic acid-aspirin conjugate can be used for reducing experimental pulmonary metastasis of 4T1 breast cancer of a rat and showing a relatively good tumor metastasis resisting effect. The ursolic acid-aspirin conjugate provided by the invention can be applied to preventive drugs for tumor metastasis, thereby providing novel selection for developing new drugs for tumor metastasis and preventing postoperative retransfer of malignant tumors. The formula (I) is shown in the description.

The invention relates to an Ursolic Acid (UA) derivative. The Ursolic Acid (UA) derivative is a NO-donating UA derivative formed by coupling ester bonds or amido bonds of Ursolic Acid (UA) which has anti-tumor activity with NO donators in different types by connecting groups; and external primary screening result shows that the toxic effect of a plurality of compounds on HepG2 is evidently stronger than UA, therefore, the Ursolic Acid (UA) derivative has the prospect of anti-tumor application.

The invention discloses a comprehensive extraction technology of rosmarinic acid, ursolic acid, oleanolic acid and carnosic acid. The comprehensive extraction technology comprises the steps of (1) preprocessing raw materials; (2) performing reflux alcohol-extraction; (3) carrying out primary separation; (4) separating out rosmarinic acid; (5) separating out ursolic acid; (6) separating out oleanolic acid. According to the comprehensive extraction technology, the process is simple, the operation is convenient, industrial production can be easily carried out, and meanwhile, the purpose of synchronously extracting rosmarinic acid of rosemary, ursolic acid, oleanolic acid and carnosic acid is achieved, and the active ingredients of rosemary can be extracted to the maximum; the rosmarinic acid, ursolic acid, oleanolic acid and carnosic acid respectively have the purity exceeding 30%, 25%, 20% and 25%.

The present invention is a method for preparing triterpenoids such as 2-cyano-3,12-dioxoolean-1,9-dien-28-methyl ester and derivatives thereof from oleanic acid, ursolic acid, betulinic acid, sumaresinolic acid or hederagenin.

The invention relates to dual-targeting ursolic acid (UA) / siRNA loaded fluorescent mesoporous silica dioxide-hyaluronic acid and application. The technical scheme of the invention lies in that 1, fluorescently-labeled mesoporous silica dioxide nano particles are synthesized through the template method; 2, the surface of FMSN is subjected to amino modification through 3-aminopropyltriethoxysilane; 3, ursolic acid (UA) and siRNA are jointly loaded into porous channels of the nano particles; 4, hyaluronic acid is loaded into the outer surfaces of the nano material in a wrapping manner through electrostatic absorption, so as to obtain the dual-targeting ursolic acid (UA) / siRNA loaded fluorescent mesoporous silica dioxide-hyaluronic acid nano particles. According to the dual-targeting ursolic acid (UA) / siRNA loaded fluorescent mesoporous silica dioxide-hyaluronic acid and application, the prepared nano material is uniform in particle size distribution, and good in dispersibility, the stability of siRNA can be improved, and besides, siRNA can further specifically target tumor cell surface adhesion molecules like ICAM and CD44, therefore the anti-tumor effect of drugs can be greatly improved, and the toxic and side effect of the drugs can be further reduced.

The invention discloses a carrier-free co-assembled tumor targeting anti-cancer nano medicine as well as a preparation method and application thereof. The carrier-free dual anti-cancer nano medicine is prepared from a hydrophobic medicine, namely ursolic acid, together with board-spectrum anti-tumor medicines such as doxorubicin in water through co-assembling, in addition, a fluorescence labeling nucleic acid aptamer, a molecular target, an antibody or polypeptide and the like with tumor targeting functions are adsorbed to the surface of the medicine through mutual electrostatic functions, then the carrier-free co-assembled tumor targeting anti-cancer nano medicine with tumor targeting and tumor microenvironment response is prepared, a synergic anti-tumor function is achieved, diagnosis and treatment integration is achieved, particularly the medicine has outstanding functions in preventing tumor transfer, and more importantly the problems that a conventional nano carrier is complex in system, indefinite in in-vivo metabolism and the like are solved.

The invention relates to a method for combinedly extracting Morroniside, dogwood polysaccharide and ursolic acid from dogwood. The method comprises the following steps of: crushing the dogwood; adding water in an amount which is 10 to 15 times of the weight of the dogwood and performing heating extraction for 2 to 3 times; mixing the extracting liquid; loading the extracting liquid to a macroporous resin column I for adsorption; collecting liquid which flows down the column; eluting by using water and ethanol solution sequentially; mixing the water eluent with the liquid which flows down the column; adding alcohol for precipitation; filtering precipitates out and drying to obtain the dogwood polysaccharide; collecting the alcohol eluent, concentrating until alcohol does not exist, adding water for dispersion, adding isometric n-butyl alcohol for extraction, concentrating an n-butyl alcohol layer to recover the n-butyl alcohol, and drying to obtain the Morroniside; adding alcohol into the residues obtained after water extraction to perform reflux extraction, concentrating the extracting liquid, loading the extracting liquid to a macroporous resin column II for adsorption, eluting by using 90 percent ethanol; and adding active carbon into the eluent to perform reflux decoloring, filtering the active carbon out, cooling naturally for crystallization, filtering out crystals, and drying to obtain the ursolic acid. In the method, toxic reagents do not exist in the preparation process; the process is simple; the utilization ratio of the raw materials is high; cost is low; and the method is suitable for industrialized production.

The invention provides a method for separating the ursolic acid from the folium eriobotryae, which is characterized in that: having the folium eriobotryae as material, extracting in the water solution by the hydrophiled organic solvent, decompressed recovering the solvent, concentrating the folium eriobotryae total triterpenic acid extract; removing the impurities by solvent, active carbon decoloring, precipitation separation of the triterpenic acid extract to produce the coarse folium eriobotryae total triterpenic acid extract with the ursolic acid concentration >=25%; enrichment and purification, precipitation separation to the beige coarse ursolic acid with the ursolic acid concentration >=80%; extraction by hydrophilic organic solvent, crystalline purification and recrystallization of the coarse ursolic acid, and finally getting the purified ursolic acid crystal. The purity of the white ursolic acid crystal is >=99%. The invention has a simple producing process, a high separation efficiency, a low cost and a good extension.

The invention discloses a method for extracting high-purity ursolic acid from rosemary, which is characterized by comprising the following steps of: A, adding ethanol into the leftovers obtained after grinding rosemary leaves and extracting carnosic acid for extraction; filtering and obtaining the filtrate; adding active carbon into the filtrate for decoloration; filtering and concentrating; adding 2-5 times water by volume while stirring to separate out precipitate; filtering to obtain a crude extract of ursolic acid; B, adding an organic solvent into the crude extract of ursolic acid in the step A for dissolution; adding diethylamine or triethylamine for backflow reaction to generate salt; and cooling to room temperature and filtering to obtain amine salt; C, adding water into the amine salt in the step B to dissolve the amine salt; adding isopropyl ether; adjusting the pH value to 2-5 by dilute acid; keeping the temperature at 20-40 DEG C for 1 hour; filtering; and drying the filter cake; and D, adding methanol into the dried filter cake in the step C for dissolution; adding neutral alumina for decoloration; filtering and concentrating the filtrate; cooling to minus10-20 DEG C for crystallization; and centrifuging, washing and drying to obtain the finished product of ursolic acid.