103 results about "Daidzin" patented technology

The invention relates to an action of ferulic acid on enhancing the drug effect of some medicaments and a purpose thereof, wherein the medicaments mainly comprise alkaloid medicaments including matrine, kushenin, sophocarpine, hyoscyamine, narceine, hanfangchin A, jamaicin, morphine, codeine, evodiamine, strychnine, catharanthine, vincristine, taxol, verticine, peimine, peiminine, ephedrine, pseudoephedrine, wilfordine, triptolide, tripdiolide and the like, and flavonoid medicaments including puerarin, ginsenoside, ginsengenin, mangiferin, scutelloside, alkannin, meletin, rutin, hesperidin, daidzin, soybean isoflavone, daidzein, carthamin, catechin and the like. The ferulic acid and the medicaments can form a compound or a medicament compound, or the ferulic acid and the medicaments can generate a chemical reaction (including salification, esterification, amidation, ketonization, etherification and the like), and / or the ferulic acid and the medicaments can generate a synergistic effect and an additive effect.

A UGT2B inhibitor capable of increasing the bio-availability of a drug, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: capillarisin, isorhamnetin, β-naphthoflavone, α-naphthoflavone, hesperetin, terpineol, (+)-limonene, β-myrcene, swertiamarin, eriodictyol, cineole, apigenin, baicalin, ursolic acid, isovitexin, lauryl alcohol, puerarin, trans-cinnamaldehyde, 3-phenylpropyl acetate, isoliquritigenin, paeoniflorin, gallic acid, genistein, glycyrrhizin, protocatechuic acid, ethyl myristate, umbelliferone, PEG (Polyethylene glycol) 400, PEG 2000, PEG 4000, Tween 20, Tween 60, Tween 80, BRIJ® 58, BRIJ® 76, Pluronic® F68, Pluronic® F127, and a combination thereof. A UGT2B enhancer capable of enhancing a clearance rate of morphine-like analgesic agents, is a compound in a free base or a pharmaceutically acceptable salt form that is selected from the group consisting of: nordihydroguaiaretic acid, wogonin, trans-cinnamic acid, baicalein, quercetin, daidzein, oleanolic acid, homoorientin, hesperetin, narigin, neohesperidin, (+)-epicatechin, hesperidin, liquiritin, eriodictyol, formononetin, quercitrin, genkwanin, kaempferol, isoquercitrin, (+)-catechin, naringenin, daidzin, (−)-epicatechin, luteolin-7-glucoside, ergosterol, rutin, luteolin, ethyl myristate, apigenin, 3-phenylpropyl acetate, umbelliferone, glycyrrhizin, protocatechuic acid, poncirin, isovitexin, 6-gingerol, cineole, genistein, trans-cinnamaldehyde, and a combination thereof.

The invention discloses a process for extracting and separating isoflavones from kudzu vine, which comprises the steps of disintegrating kudzu vine to 60-150 meshes, loading into a liquid dynamic continuous flow upstream extraction device for alcohol extraction, combining the extract liquid and filtering, concentrating the filtrate to obtain total isoflavones extract, and separating the total isoflavones extract to obtain puerarin and soybean aglycone.

The invention relates to method for preparing superfine arrowroot powder. The preparation method comprises the steps of removing the outer peel of the arrowroot, slicing the arrowroot; soaking the slices in 100mg / kg sterilizing liquid made from chlorine dioxide for 15-30min; subjecting the slices to protective coloration in preservative liquid with 0.3% of citric acid and 0.9% of sodium chloride by mass percentage; removing water; killing enzyme in water of 95 DEG C for 2-3min; removing water again; drying the slices through hot air of 115 plus or minus 5 DEG C to get the slices with water content accounting for 8% plus or minus 1%; pulverizing the dried arrowroot slices into 800-1000meshes through a micronizer; and weighing and packing, or adding 1% of aspartame which is 200 times of sucrose saccharinity into the pulverized arrowroot slices, and then weighing and packing. Compared with traditional methods, the method can avoid the loss of three functional ingredients, such as puerarin, daidzein and daidzin, amd increase yield. The produced arrowroot powder is suitable for use in cooking food.

The invention belongs to the technical field of microorganism and discloses a clostridium bifermentans for generating equol by degrading daidzein and bacteria agent and application thereof. The clostridium bifermentans for generating equol by degrading daidzein zx-7 is collected in the China General Microbiological Culture Collection Center, and the collection number is CGMCC NO.1995. The clostridium bifermentans CGMCC NO.1995 can degrade daidzein to generate equol, the low density lipoprotein content of rat serum is remarkably reduced, the ratio of the high-density lipoprotein to the low-density lipoprotein is obviously increased, the elimination of cholesterol in rat is facilitated, and the host health is favored. The clostridium bifermentans can be used for preparing animal feed, a preparation for eliminating cholesterol and a fermented product of soy or bean pulp.

The present invention is the method of extracting effective components in kudzu vine root. The present invention features that during the separation of effective components in kudzu vine root, S-8 resin is used to adsorb the effective components of the concentrated extractive and mounted in chromatographic column for elution with ethanol or methanol solution of 30-50 %, 50-80 % and 80-100 % concentration separately, and the three kinds of eluted liquid are collected separately and concentrated, crystallized and dried separately. The said process can extract and separate the three kinds of effective components, including puerarin, daidzin and daidzein simultaneously and this can raise the utilization value and make full use of the Chinese medicine resource.

The invention is a method to separate main monomer components in soy isoflavone, using the mixture with not less than 40% soy isoflavone content as raw material, silica gel as sorbent, and chloroform-methanol solution as eluting system, its technical steps: preparing sample solution; processing sorbent and placing in column; applying sample, where the added quantity of sample solution is 5-15% of effective volume of separating column; eluting, where the eluent flow velocity is controlled at 0.5-1.5% of effective volume of separating column each minute, and collecting the eluent in sections; qualitatively detecting the collected eluents, respectively and compared with the contrast product; obtaining monomer components, combining the eluents with the same component, and respectively vacuum condensing and drying to obtain four main monomer components: genistein, daidzein, genistin and daidzin. It is detected by high efficiency liquid phase chromatography that each monomer component's content can reach above 90%.

The invention discloses a series health-care food containing pearl and kudzu root and a preparation method thereof. The food is prepared by matching natural pearl and kudzu root which can be taken both as food and medicine with vitamin, medlar, a sweetening agent and appropriate accessories according to a certain proportion. The health-care food contains organic calcium, inorganic calcium, vitamin and a plurality of trace elements which are needed by human body, and the food further contains components such as flavonoid daidzin, daidzein, xylopuerarin, kudzu powder flavin, and great amount of starch, therefore, the food has the functions of beautifying the face and enlarging the breast, nourishing the tendons and channels and preventing cerebral-cardiovascular diseases, and is taken as health products which are fit for women, the middle aged people and the old, thus delaying senility, strengthening the body and maintaining the beauty and youth of skin.

A process for preparing high-purity kakonein and daidzin from pueraria root includes mixing puerania root with the aqueous solution of ethanol, heating while reflux extracting, centrifugal separation to emove dregs, concentrating, acidolyzing, extracting active component by ethyl acetate, concentrating for crystallizing, filtering to separate acidic liquid, recrystallizing in purified water or alcohol, drying to obtain high-purity daidzin, concentrating separated acidic liquid, crystallizing, filtering, recrystallizing in purified water or alcohol, and drying to obtain high-purity kakonein.

The invention provides a hair-growing agent composition, which is capable of treating exiguous human hair, calvities occurring in females due to lowering of the estrogen concentration along with the aging by using soybean isoflavones aglycone and 6-benzyl amino purine at the same time, and has great calvities prevention effect while having hair-growing effect. The invention provides a hair-growing agent composition containing soybean isoflavones aglycone and 6-benzyl amino purine. An ideal condition is that the soybean isoflavones aglycone is any one from the daidzin, genistin, and the mol ratio between soybean isoflavones aglycone (A) and 6-benzyl amino purine is 1:1000000 - 1000:1 or the like.

The invention discloses Enterococcus faecium BY1, a method for producing equol by using the Enterococcus faecium and application of the Enterococcus faecium, and belongs to the field of biochemical industry. The bacteria were collected in China General Microbiological Culture Collection Center on March 28, 2011, and the collection number is CGMCC NO.4707. The Enterococcus faecium has the capacity of transforming a daidzin part in isoflavone into the equol, and can be used for preparing food containing the equol.

The invention provides a method to detect effective ingredients of radices puerarire, radices scutellariae and coptis chinensis medicine (Gegen Qin Lian Medicine) by high efficiency liquid chromatogrophy. The method consists of the following steps: firstly, a sampling liquid to be detected and a mixing control solution are prepared, which are then respectively detected by the following methods: a chromatographic column stuffed by octadecyl silane bonded silica gel is injected by the sample size of 1 to 20ul, the column temperature is controlled between 10 to 40 DEG C, then gradient elution is carried out by a mobile phase which is composed of phase A and phase B at a flow velocity of 0.3 to 1.5ml / min according to the stipulated procedure; moreover, multi-wavelength ultraviolet is employed to detect the eluent so as to obtain the high efficiency liquid chromatograms of the detected medicines and the control solution; finally, the high efficiency liquid chromatograms of the detected medicines and the control solution are compared so as to determine the effective components of puerarin, daidzin, wogonoside, baicalin, palmatine and berberine and the contents thereof. The phase A in the method of the invention is ammonium acetate solution with the concentration of 0.01 to 0.1mol / l, and the phase B is acetonitrile.

The invention provides a salmon and trout feed based on a peroxisome proliferator activated receptor (PPAR) and a method for preparing the salmon and trout feed. The salmon and trout feed comprises proteins, fat, trace elements and other basic substances which are required by the salmon and trout growth, and further contains natural PPAR alpha and / or PPAR beta binding active substances, such as daidzin, curcumin, isoflavone, arachidonic acid, berberine, naringenin, polydatin, kaempferol, isoflavoues aglycone, conjugated linoleic acid and the like. The salmon and trout feed can regulate the fat and fatty acid anabolism of the cultured salmon and trout, the fat content of the salmon and trout is lowered, the beneficial omega-3 fatty acid content is improved, and the salmon and trout quality is improved.

The invention discloses a method for quickly determining six soy isoflavones in a bean product by utilizing UPLC (Ultra Performance Liquid Chromatography), and belongs to the technical field of analytical chemistry. The types of the tested bean product comprise soybean milk, bean curd, soy chicken, dried tofu, bean curds, dried beancurd sticks and the like, and the six soy isoflavones such as daidzin, isoflavoues Aglycone, Glycitin, glycitein, genistin and genistein are determined. The method comprises the following steps: firstly extracting the soy isoflavones in the bean product by using a methanol solution, after collecting a supernate fluid, repeatedly extracting residual twice by using methanol, merging the supernate fluids, and carrying out UPLC detection. Quick determination for the composition and content of the six soy isoflavones mainly in the bean product are achieved by adopting UPLC. The method is simple in sample pretreatment, high in analysis speed, high in repeatability and accuracy, and provides an efficient and accurate analytical method means for evaluating and controlling the quality of the bean product.

The present invention provides a UPLC method for qualitatively and quantitatively detecting the contents of six components such as 3'-hydroxy puerarin, puerarin, puerarin-6"-O-xyloside, 3'-methoxy puerarin, mirificin and daidzin in radix puerariae, radix puerariae extracts and radix puerariae-containing preparations by adopting puerarin as a reference substance. The UPLC method comprises: preparing a reference substance solution and a testing sample solution, and carrying out gradient elution by adopting 0.1% acetic acid as a mobile phase A and adopting acetonitrile as a mobile phase B, wherein the detection wavelength is 250 nm, the chromatographic column is SB-C18, RRHD 1.8 [mu]m, 2.1*100 mm, the column temperature is 31 DEG C, the flow rate is 0.4 mL / min, and the injection volume is 1 [mu]L; comparing the obtained chromatogram with a radix puerariae herb finger print, wherein the main peak is puerarin, and other components are corresponding to various peaks in a one-to-one manner so as to identify the herb; and calculating the contents of the six components in the radix puerariae by combining relative calibration factors (the relative calibration factor of 3'-hydroxy puerarin is 1.253, the relative calibration factor of puerarin is 1.000, the relative calibration factor of puerarin-6"-O-xyloside is 1.422, the relative calibration factor of 3'-methoxy puerarin is 1.297, the relative calibration factor of mirificin is 1.332, and the relative calibration factor of daidzin is 1.030) and the peak area of puerarin in the contrast solution.

The invention relates to a rapidly-available beverage capable of dispelling the effects of alcohol and nourishing the stomach and belongs to the technical field of processing of health-care beverages. The rapidly-available beverage capable of dispelling the effects of alcohol and nourishing the stomach is prepared from tomato juice, kudzu root extracting solution, daidzin, vitamin B, citric acid, xylitol, dihydromyricetin, aspartame and clear spring water in a certain proportion. The rapidly-available beverage capable of dispelling the effects of alcohol and nourishing the stomach can be used for reducing the damages of acetaldehyde to liver, brain and central nervous systems, eliminating the headache, skin allergy and multi-day continuous uncomfortableness after intoxication and effectively eliminating the fatigue, is dulcitude in taste, and is free from any side effect on a human body since purely natural tomato juice is used as a main raw material.

The invention provides a preparation method of a natural pueraria radix juice and a method for preparing a pueraria radix drink with the natural pueraria radix juice. In the preparation method of the natural pueraria radix juice, a device with temperature chromatographic separation technology function is used for preparing the natural pueraria radix juice, wherein, the natural pueraria radix juice takes the materials with high starch content such as pueraria radix as raw materials. The device with temperature chromatographic separation technology function comprises a heating zone and a precipitating zone. In the prepared natural pueraria radix juice, the starch content is equal to or less than 0.4%, the content of puerarin in isoflavones is 0.05%-0.07% and the total content of daidzeol and daidzin is 0.005%-0.01%. The pueraria radix drink can contain one or two of koumiss and agaricus blazei water, or contain no additives.

The invention discloses a process for extracting and separating isoflavones from kudzu slag, which comprises the steps of disintegrating kudzu slag to 60-150 meshes, loading into a liquid dynamic continuous flow upstream extraction device for alcohol extraction, combining the extract liquid and filtering, concentrating the filtrate to obtain total isoflavones extract, and separating the total isoflavones extract to obtain puerarin and soybean aglycone.

The invention discloses novel beta-glucosidase. The beta-glucosidase is characterized in that a nucleotide sequence is shown as SEQ ID NO.1, and an amino acid sequence is shown as SEQ ID NO.3. The invention also discloses an expression vector containing the novel beta-glucosidase, recombinant beta-glucosidase, and an application of recombinant beta-glucosidase in efficient hydrolyzing of soybean isoflavone glycoside. The novel recombinant beta-glucosidase shows efficient soluble expression in an escherichia coli expression system, can react preferably at a temperature of 45 DEG C under pH (Power of Hydrogen) of 6.0, and is high in heat stability and wide in pH enzymolysis range; and in addition, the recombinant beta-glucosidase can efficiently hydrolyze daidzin and genistin, and the hydrolyzing efficiency is 100%.

The invention discloses a Chinese medicament for treating acute enteritis. The Chinese medicament for treating acute enteritis is prepared from 20 Chinese medicaments in a certain weight part ratio, namely humifuse euphorbia herb, malt, baicalin, rice sprout, puerarin, plantain seed, berberine, bitter orange, rhizome of Chinese atractylode, jateorhizine, phoenix-tail fern, coix seed, palmatine, amomum fruit, honeysuckle, weeping forsythia, dandelion, glycyrrhetic acid, gordon euryale seed and daidzin. The Chinese medicament for treating acute enteritis has the characteristics of low cost, relatively high curative ratio and the like, and is convenient to prepare, and materials are easy to obtain.

The invention belongs to the field of medicine and natural product chemistry, and particularly relates to a novel isoflavone glycoside compound and a preparation method and application thereof. The isoflavone glycoside compound has the following structure shown in the description, normal phase (silica gel) column chromatography separation is adopted, reverse (open ODS) column chromatography separation is adopted, and a compound 2'-hydroxyl daidzin is obtained through methanol recrystallization finally. The novel isoflavone glycoside compound can be used for preventing and treating various diseases caused by the fact that too many free radicals in the human body are produced, or the capacity of eliminating the free radicals is decreased. Please see the formula in the description.

The invention provides a Chinese herbal medicine preparation pesticide and a production method thereof. The pesticide comprises a solvent and a solute, and the solute comprises brassinolide, sophocarpidine, berberine, potassium fulvate, quercitrin, chlorogenic acid, ophiopogonin, arginine, echinacoside, liquiritin and daidzin. Solute components are extracted from plants containing the solute components through multiple times of water decoction by a traditional Chinese medicine decoction method, an aqueous solution is formed after concentration, and then a surfactant and an auxiliary agent areadded or filtration is performed to obtain the Chinese herbal medicine preparation pesticide. The pesticide is used for plantation of Chinese herbs, fruits and vegetables, diseases can be prevented, and planting quality can be improved.

The invention discloses a high-temperature resistant flavonoid aglycone invertase and applications thereof. Amino acid sequence of the high-temperature resistant flavonoid aglycone invertase is represented by SEQ ID No.1. Thermal stability of the high-temperature resistant flavonoid aglycone invertase is excellent; the high-temperature resistant flavonoid aglycone invertase is capable of enduring high temperature, and possesses relatively excellent catalytic conversion capability on flavone glycosides of different mother nucleus types and different glucosidic bond types. After a certain time of incubation of the high-temperature resistant flavonoid aglycone invertase with typical flavone glycosides (isoquercetin, astragalin, daidzin, and saprosma ternatum glucoside), it is observed via detection that almost all of the flavone glycosides are converted into corresponding flavonoid aglycones (quercetin, kaempferol, isoflavoues aglycone, and genistein).

The invention provides polypeptide tea for blood glucose reduction and a manufacturing method of the polypeptide tea. The tea comprises a bitter gourd extract, a rhizoma polygonati odorati extract, and a radix puerariae extract, and is abundant in effective components including vitamins, trace elements, amino acids, tea polysaccharide, rhizoma polygonati odorati polysaccharide, steroidal glycoside, daidzein, daidzin, puerarin, and puerarin-7-xyloside, and has the efficacy of clearing heat and engendering fluid, reducing blood glucose and wiping off grease, and resolving stasis and free the collateral vessels. The tea is suitable for patients who has abnormal glucose tolerance and suffers from slight diabetes, and has the efficacy of reducing fasting blood glucose. The polypeptide tea for blood glucose reduction has properties as medicines and takes like tea, and the formula of the tea is based on the traditional Chinese medicine curative theory of yin-yang balance and healthy qi reinforcement.

The invention discloses an anti-fatigue health care wine. The optimal anti-fatigue component of the health-care wine is composed of the following components in percentage: 0.8-1.5% of puerarin, 0.2-0.4% of daidzin, 0.008-0.020% of calycosin-7-glucoside, 0.01-0.04% of ononin, 0.02-0.06% of icarisid II, 0.004-0.009% of p-coumaric acid, 0.004-0.009% of baohuoside II, and 0.04-0.08% of daidzein. The eight anti-fatigue major functional components in the health care wine are screened out through a zebra fish model, and the optimal proportion of the eight components is further determined by utilizingthe zebra fish model. Experiments show that the zebra fish is administrated according to the optimal formula, and thereby the anti-fatigue effect of the zebra fish is the best.