Beta-glucosidase gene for efficiently hydrolyzing soybean isoflavone glycoside and application thereof

A glucosidase, purpose technology, applied in application, genetic engineering, plant genetic improvement, etc.

Inactive Publication Date: 2014-05-14
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

So far, there is no research report on the use of metagenomic technology to obtain a new type of β-glucosidase from seabed mud with high-efficiency hydrolysis of soybean isoflavone glycosides

Method used

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  • Beta-glucosidase gene for efficiently hydrolyzing soybean isoflavone glycoside and application thereof
  • Beta-glucosidase gene for efficiently hydrolyzing soybean isoflavone glycoside and application thereof
  • Beta-glucosidase gene for efficiently hydrolyzing soybean isoflavone glycoside and application thereof

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Abstract

The invention discloses novel beta-glucosidase. The beta-glucosidase is characterized in that a nucleotide sequence is shown as SEQ ID NO.1, and an amino acid sequence is shown as SEQ ID NO.3. The invention also discloses an expression vector containing the novel beta-glucosidase, recombinant beta-glucosidase, and an application of recombinant beta-glucosidase in efficient hydrolyzing of soybean isoflavone glycoside. The novel recombinant beta-glucosidase shows efficient soluble expression in an escherichia coli expression system, can react preferably at a temperature of 45 DEG C under pH (Power of Hydrogen) of 6.0, and is high in heat stability and wide in pH enzymolysis range; and in addition, the recombinant beta-glucosidase can efficiently hydrolyze daidzin and genistin, and the hydrolyzing efficiency is 100%.

Description

technical field [0001] The invention relates to a new beta-glucosidase gene, in particular to a beta-glucosidase gene for efficiently hydrolyzing soybean isoflavone glycosides, a recombinase and the application of the recombinase, belonging to the field of genetic engineering. Background technique [0002] β-glucosidase (β-glucosidase) belongs to the glycoside hydrolase family, also known as β-D-glucoside glucohydrolase (β-D-glucosideglucohydrolase; EC3.2.1.21), on the one hand, the enzyme can catalyze the hydrolysis of β - a glycosidic bond, releasing glucose and the corresponding aglycone. On the other hand, the enzyme can also catalyze the transglycoside condensation reaction to synthesize oligosaccharides. The study found that the free type soybean isoflavone glucoside can exert its biological activity, and β-glucosidase plays an important role in catalyzing the hydrolysis of the bound type glycoside to produce the free type glycoside, which can significantly improve th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/56C12N9/42C12N15/70C12N1/21C12N15/10C12P19/02C12P17/06C12R1/19
Inventor 刘玉焕童铃曹立创李良郭耿珊汪思迪
Owner SUN YAT SEN UNIV
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