586 results about "Glycosidic bond" patented technology

The present invention provides an alginate oligosaccharide and its derivatives with the degree of polymerization ranging from 2 to 22. The alginate oligosaccharide is composed of β-D-mannuronic acid linked by 1,4 glycosidic bonds. The derivatives with the reduced terminal in position 1 of carboxyl radical can be prepared by oxidative degradation. The present invention also provides a process for preparing the alginate oligosaccharide and its derivatives, which includes the procedures that an alginate solution is reacted for 2 to 6 h in an autoclave at pH 2-6 and the temperature of 100-120° C., and pH is adjusted to 7 after the reaction is stopped, after which the resultant oligosaccharide is oxidized in the presence of an oxidant to obtain an oxidative degradation product. The alginate oligosaccharide and its derivatives of the invention can be used in the manufacture of a medicament for the prophylaxis and treatment of AD and diabetes.

The invention relates to polypeptides having glucanase, e.g., endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (e.g., endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (e.g., carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4 bonds in mixed beta-1,3 glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases e.g., endoglucanases, mannanases, xylanases have increased activity and stability, including thermotolerance or thermostability, at increased or decreased pHs and temperatures.

A method for enhancing a binding activity of an antibody composition to Fcγ receptor IIIa, which comprises modifying a complex N-glycoside-linked sugar chain which is bound to the Fc region of an antibody molecule; a method for enhancing an antibody-dependent cell-mediated cytotoxic activity of an antibody composition; a process for producing an antibody composition having an enhanced binding activity to Fcγ receptor IIIa; a method for detecting the ratio of a sugar chain in which fucose is not bound to N-acetylglucosamine in the reducing end in the sugar chain among total complex N-glycoside-linked sugar chains bound to the Fc region in an antibody composition; an Fc fusion protein composition produced by using a cell resistant to a lectin which recognizes a sugar chain in which 1-position of fucose is bound to 6-position of N-acetylglucosamine in the reducing end through α-bond in a complex N-glycoside-linked sugar chain; and a process for producing the same.

A method for the anticoagulant and / or antithrombotic treatment of a human or other warm-blooded animal patient in need of such treatment, comprises administration to the patient of an effective amount of at least one sulfated oligosaccharide, wherein the oligosaccharide has the general formula I:wherein R1 and R2 and each Rx represents a monosaccharide unit, all of which may be the same or different, adjacent monosaccharide units being linked by 1->2, 1->3, 1->4 and / or 1->6 glycosidic bonds andn is an integer of from 1 to 6.

The invention relates to a Microorganisms or / and biological enzyme extracting method for plant anthocyanin, belonging to the technical field of biological engineering. The method comprises the following steps of: classifying plants containing anthocyanin for smashing, adsorbing water, freezing the plants, heating the plants for thawing, pressing the plants, filtering for making juice, cracking and degrading the anthocyanin by the microorganisms or / and the biological enzyme cracking anthocyanin glycosidic bond, utilizing an anion active agent for precipitation reaction, degrading the precipitate by the microorganisms, and carrying out precipitation separation and evaporation concentration. Cation anthocyanin with the real content of more than 95%, namely color primitive but not the anthocyanin can be obtained by the technology of the invention. The cation anthocyanin made by the invention does not need human intestinal microorganisms for degrading, and is directly adsorbed and utilizedby a human body or directly goes into human blood. The medical care value of the plant anthocyanin is much higher than that of the anthocyanin.

The object of the present invention is to provide an α-isomaltosyl-transferring enzyme which forms a cyclotetrasaccharide having the structure of cyclo{→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→} from a saccharide having a glucose polymerization degree of at least three and having both the α-1,6 glucosidic linkage as a linkage at the non-reducing end and the α-1,4 glucosidic linkage other than the linkage at the non-reducing end; microorganisms which produce the enzyme; process for producing the enzyme; cyclotetrasaccharide or saccharide compositions comprising the same; and uses thereof.

A carbon-based solid acid which has high activity and high thermal stability and is useful as an acid catalyst for various reactions such as hydration of olefins.The carbon-based solid acid for use as a catalyst is obtained by carbonization and sulfonation of an organic substance, which has a reduction rate of 10 mol % or less of acid content as measured by immersing the solid acid in hot water at 120° C. for 2 hours, is used as the acid catalyst.The organic substance to be used as the raw material for preparing the solid acid is preferably a saccharide having β1-4 glycosidic bond (e.g. cellulose) or lignin. Amylose is also suitable as the raw material. Examples of the reaction for which the solid catalyst can be used include hydration of olefins, etherification of olefins, and acid / alcohol esterification.

The invention relates to the field of preparation of marine active substance from byproducts processed by aquatic animals and aims at providing a process for extracting collagen and dermatan sulfate by comprehensively utilizing marine fish skins. The process comprises the steps of fish skin treatment, removal of impure protein of the fish skin, removal of fat of the fish skin, fish skin enzymolysis, collagen salting, collagen collection, collagen dialysis and lyophilization, dermatan sulfate glucosidic bond protection, dermatan sulfate precipitation, dermatan sulfate purification and the like. According to the process disclosed by the invention, the active substances can be extracted from the marine fish skins simply, quickly and efficiently, the aim of cogenerating the collagen with the dermatan sulfate is achieved, the utilization benefits of the fish skins are increased and economic benefits are increased. The process is simple and feasible, high in extraction efficiency and suitable for industrial production.

The invention provides a lactobacillus plantarum exopolysaccharide. According to the exopolysaccharide, sugar units in the molecular structure are only xylose and galactose, the galactose accounts for 98%, and the exopolysaccharide belongs to a lactobacillus plantarum exopolysaccharide of a brand new structure. Meanwhile, the invention provides a method for preparing the exopolysaccharide. The method comprises the following steps: precipitating polysaccharides in lactobacillus plantarum fermentation solution by ethanol, dialyzing to remove small molecules, removing nucleic acid and protein impurities by the enzymolysis methods; precipitating impurity proteins by trichloroacetic acid, performing solid-liquid separation, taking the supernatant, and performing dialysis, thereby obtaining the purified target polysaccharide. According to the method, due to the property of the target polysaccharide and impurity component design process parameters of lactobacillus plantarum fermentation solution, the temperature and pH conditions are mild, purification methods capable of possibly breaking polysaccharide cross-linked bonds such as ultrafiltration are avoided, and integrity of polysaccharide glycosidic bonds and secondary structures is effectively guaranteed. The polysaccharide prepared by the method is high in purity and yield and lays a foundation for subsequent performance analysis and industrial application.